JPS6429756A - Production of gradient gel membrane for electrophoresis - Google Patents

Production of gradient gel membrane for electrophoresis

Info

Publication number
JPS6429756A
JPS6429756A JP62183715A JP18371587A JPS6429756A JP S6429756 A JPS6429756 A JP S6429756A JP 62183715 A JP62183715 A JP 62183715A JP 18371587 A JP18371587 A JP 18371587A JP S6429756 A JPS6429756 A JP S6429756A
Authority
JP
Japan
Prior art keywords
slot
coating
groove
liquid
specified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62183715A
Other languages
Japanese (ja)
Inventor
Fumitaka Terai
Kimio Yugawa
Mineo Suefuji
Isamu Iwamoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Holdings Corp
Original Assignee
Fuji Photo Film Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fuji Photo Film Co Ltd filed Critical Fuji Photo Film Co Ltd
Priority to JP62183715A priority Critical patent/JPS6429756A/en
Publication of JPS6429756A publication Critical patent/JPS6429756A/en
Pending legal-status Critical Current

Links

Landscapes

  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To produce a gel membrane at the medium liquid concn. and coating film thickness uniform over the entire coating width by coupling a static mixer directly to a coating head and using a modifying agent which does not contain urea. CONSTITUTION:The coating head 8 mounts a medium liquid 12 for electrophoretic sepn. which is a coating liquid from the static mixer 7 directly to a supply port 14. The liquid is refracted by passing an expanding slot 16a to constitute a parallel slot 16 and is coated from a slot aperture 17 onto a web 13 on a coating roller 20. The position of the slot aperture 17 is set in a range of the position beta=0-90 deg. on the roller 20. The transverse spreading angle theta of the expanding slot 16a is specified to <=40 deg.. A groove 25 as a transverse distribution chamber to apply transient expansion to the liquid near the terminal of the slot 16a is provided in order to uniformize the thickness in the transverse direction. The depth D of the groove 25 is specified to <=2C (C is a slot clearance), the distance K to the parallel slot 16 of the groove 25 to <=5mm and the height L of the inverted triangular shape of the groove 2 to 5-20mm.
JP62183715A 1987-07-24 1987-07-24 Production of gradient gel membrane for electrophoresis Pending JPS6429756A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62183715A JPS6429756A (en) 1987-07-24 1987-07-24 Production of gradient gel membrane for electrophoresis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62183715A JPS6429756A (en) 1987-07-24 1987-07-24 Production of gradient gel membrane for electrophoresis

Publications (1)

Publication Number Publication Date
JPS6429756A true JPS6429756A (en) 1989-01-31

Family

ID=16140691

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62183715A Pending JPS6429756A (en) 1987-07-24 1987-07-24 Production of gradient gel membrane for electrophoresis

Country Status (1)

Country Link
JP (1) JPS6429756A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6411190B1 (en) 1999-08-03 2002-06-25 Yazaki Corporation Circuit breaker
US6483420B1 (en) 1999-08-03 2002-11-19 Yazaki Corporation Circuit breaker

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6411190B1 (en) 1999-08-03 2002-06-25 Yazaki Corporation Circuit breaker
US6483420B1 (en) 1999-08-03 2002-11-19 Yazaki Corporation Circuit breaker

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