JPS6399090A - Kbs3-p1004 substance and production thereof - Google Patents
Kbs3-p1004 substance and production thereofInfo
- Publication number
- JPS6399090A JPS6399090A JP61243236A JP24323686A JPS6399090A JP S6399090 A JPS6399090 A JP S6399090A JP 61243236 A JP61243236 A JP 61243236A JP 24323686 A JP24323686 A JP 24323686A JP S6399090 A JPS6399090 A JP S6399090A
- Authority
- JP
- Japan
- Prior art keywords
- substance
- kbs3
- ether
- amino acid
- ash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000126 substance Substances 0.000 title claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 21
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 9
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 9
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 6
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 3
- 238000000921 elemental analysis Methods 0.000 claims abstract description 3
- 239000000194 fatty acid Substances 0.000 claims abstract description 3
- 229930195729 fatty acid Natural products 0.000 claims abstract description 3
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 3
- 239000003208 petroleum Substances 0.000 claims abstract description 3
- 238000000862 absorption spectrum Methods 0.000 claims description 7
- 150000001413 amino acids Chemical class 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000004380 ashing Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 101100163949 Caenorhabditis elegans asp-3 gene Proteins 0.000 abstract 1
- 101100315624 Caenorhabditis elegans tyr-1 gene Proteins 0.000 abstract 1
- 239000003429 antifungal agent Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 7
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000000843 anti-fungal effect Effects 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は抗真菌活性を有する物質ＫＢＳ３−Ｐ１００４
及びその製造法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention provides a substance having antifungal activity, KBS3-P1004.
and its manufacturing method.
本発明者らは、バチルス属菌の生産する抗菌性低分子物
質を探索中バチルス属の一菌株が抗真菌活性物質を生産
することを知り、更に、該物質が新規物質であることを
確認し、これを物質ＫＢＳ３−Ｐ１００４と命名し２本
発明を完成するに到った。While searching for antibacterial low-molecular-weight substances produced by bacteria of the genus Bacillus, the present inventors discovered that a strain of the genus Bacillus produces an active antifungal substance, and further confirmed that the substance was a new substance. This was named the substance KBS3-P1004, and the present invention was completed.
本発明で使用する菌は物質ＫＢＳ３−Ｐ１００４を生産
するものであればいずれでもよいが、例示としてはバチ
ルス（Ｂａｃｉｌｌｕｓ） ＫＢ−５３があげられる。The bacteria used in the present invention may be any species as long as it produces the substance KBS3-P1004, and an example thereof is Bacillus KB-53.
バチルスＫＢ−５３は微工研にＦＥＲＭ Ｐ−８９８６
として寄託されており、また菌学的性質の概要は次の通
りである。Bacillus KB-53 is FERM P-8986 to FIKEN.
The mycological properties are summarized as follows.
ダラム染色 十
胞子染色 十
カタラーゼテスト ＋
オキシダーゼテスト ＋
物質ＫＢＳ３−Ｐ１００４生産菌の培養培地は、資化で
きる炭素源、窒素源、ビタミン等を含有する栄養料等適
宜含有したもので、物質ＫＢＳ３−Ｐ１００４を生産蓄
積する培地であればいず九の培地でもよい。Durham staining Decaspore staining Decascatalase test + Oxidase test + The culture medium for the substance KBS3-P1004 producing bacteria contains appropriate nutrients such as assimilable carbon sources, nitrogen sources, vitamins, etc. Any culture medium that produces and accumulates may be used.
バチルスＫＢ−３３の培養に適した培地は例えば次のも
のがあげられる。Examples of suitable media for culturing Bacillus KB-33 include the following.
ポリペプトン ３０ｇ
酵母エキス ５ｇ
ＮａＣ１５ｇ
脱イオン水 ＩＱ
（ｐＨ７，０）
培養は２５〜３０℃で１通気、撹拌、振どう等好気的に
４日間程度行なわれる。Polypeptone 30g Yeast extract 5g NaC 15g Deionized water IQ (pH 7,0) Cultivation is carried out aerobically at 25-30°C for about 4 days with ventilation, stirring, shaking, etc.
得られた培養液は濾過して濾液を得、これに６Ｎ ＨＣ
Ｑを添加し、ｐＨ＝３．０に調整し沈澱物を得る。The obtained culture solution was filtered to obtain a filtrate, which was added with 6N HC.
Add Q and adjust the pH to 3.0 to obtain a precipitate.
沈澱物は水に溶解し、ＮａＨＣＯ，を加え、ｐＨ＝７．
０とし、次いでエタノールを８０％まで加える。得られ
た上清液を真空乾燥し、乾燥物を蒸留水に溶解し。The precipitate was dissolved in water and NaHCO was added to pH=7.
0 and then add ethanol to 80%. The obtained supernatant liquid was vacuum dried, and the dried product was dissolved in distilled water.
再び６Ｎ ＨＣＱを加えてｐＨ＝３．０に調整し、沈澱
物を得る。得られた沈澱物を蒸留水に溶解し、ＮａＨＣ
Ｏ。Add 6N HCQ again to adjust the pH to 3.0 to obtain a precipitate. The obtained precipitate was dissolved in distilled water, and NaHC
O.
を加え、 ＰＨ＝７．０とし、蒸留水に対して透析する
。was added, the pH was adjusted to 7.0, and the mixture was dialyzed against distilled water.
透析内液を凍結乾燥することによって物質ＫＢＳ３−Ｐ
１００４含有粗精製物を黄色粉末として得る。The substance KBS3-P was obtained by freeze-drying the dialysate fluid.
A crude product containing 1004 is obtained as a yellow powder.
得られた黄色粉末を水に溶解し、アセトニトリル系で高
速液体クロマトにかけ、これを単離し、凍結乾燥するこ
とによってほぼ純品の物質ＫＢＳ３−Ｐ１００４を白色
無定形粉末として得る。The obtained yellow powder is dissolved in water, subjected to high performance liquid chromatography using an acetonitrile system, isolated and freeze-dried to obtain an almost pure substance KBS3-P1004 as a white amorphous powder.
得られた物質ＫＢＳ３−Ｐ１００４の理化学的性質は次
の通りである。The physical and chemical properties of the obtained substance KBS3-P1004 are as follows.
１、元素分析値 Ｃ： ４８．６１％ Ｈ： ６．６２％ Ｎ ：１０．５４％ Ａｓｈ ： １．４６％ ２、灰化点 １８０〜１８２℃ ３、物質の色及び性状：白色粉体 ４、紫外線吸収スペクトル：第１図に示す通り。1. Elemental analysis value C: 48.61% H: 6.62% N: 10.54% Ash: 1.46% 2. Ashing point 180-182℃ 3. Color and properties of substance: white powder 4. Ultraviolet absorption spectrum: As shown in Figure 1.
５、赤外線吸収スペクトル：第２図に示す通り。5. Infrared absorption spectrum: As shown in Figure 2.
６、溶剤に対する溶解性：水、メタノール、エタノール
、アセトニトリルに可溶。6. Solubility in solvents: Soluble in water, methanol, ethanol, acetonitrile.
ｎ−プロピルアルコール、アセトン、酢酸エチル、エー
テル、クロロホルム、ジエチルエーテル、石油エーテル
に不溶。Insoluble in n-propyl alcohol, acetone, ethyl acetate, ether, chloroform, diethyl ether, petroleum ether.
７、呈色反応
ニンヒドリンテスト −
８、 アミノ酸組成ニアミノ酸分析機によるアミノ酸組
成は次の通りである。7. Color reaction ninhydrin test - 8. Amino acid composition The amino acid composition determined by an amino acid analyzer is as follows.
９、物質１分子あたり構造未決定の脂肪酸が１分子存在
する。9. There is one molecule of undetermined fatty acid molecule per molecule of substance.
１０、本物質は真菌に対して強い抗菌性を示す。10. This substance exhibits strong antibacterial properties against fungi.
１１、精製法：ｐ）ｌ＝３．０程度で沈澱させ、沈澱物
をＰＨ＝７．０で溶解する工程をくりかえし、最後に高
速液体クロマトにかけることによってほぼ純品を得るこ
とができる。高速液体クロマトにおける人２２０による
吸光度曲線は第３図に示す通りである。矢印が物１ＩＩ
ｔＫＢＳ３−Ｐ１００４を示している。11. Purification method: p) An almost pure product can be obtained by repeating the steps of precipitating at about l=3.0, dissolving the precipitate at pH=7.0, and finally applying to high performance liquid chromatography. The absorbance curve of human 220 in high performance liquid chromatography is as shown in FIG. The arrow is a thing 1II
tKBS3-P1004 is shown.
なお、高速液体クロマトはカラムサイズφ１．０Ｘ２０
ａｎ、ゲルは日立ゲル３０５３ （シリカ系）、溶出条
件は初発アセトニトリル４０％、トリフルオロ酢酸０．
１％の水性溶媒で、最終（４０分後）アセトニトリル６
０％、トリフルオロ酢酸０．１％の水性溶媒になる直線
濃度勾配によって行った。In addition, the column size for high performance liquid chromatography is φ1.0×20
An, the gel is Hitachi Gel 3053 (silica-based), the elution conditions are initial acetonitrile 40%, trifluoroacetic acid 0.
1% aqueous solvent, final (after 40 min) acetonitrile 6
A linear concentration gradient resulting in 0% trifluoroacetic acid and 0.1% trifluoroacetic acid in aqueous solvent was performed.
１２、抗菌スペクトラム：物質ＫＢＳ３−Ｐ１００４の
抗菌活性は次の表１に示される。12. Antibacterial spectrum: The antibacterial activity of substance KBS3-P1004 is shown in Table 1 below.
次に本発明の実施例を示す。Next, examples of the present invention will be shown.
実施例１゜
バチルスＫＢ−Ｓ３． ＦＥＲＭ Ｐ−８９８６を次の
培地に接種して、２７℃で４日間振どう培養した。Example 1 Bacillus KB-S3. FERM P-8986 was inoculated into the following medium and cultured with shaking at 27°C for 4 days.
ポリペプトン ３０ｇ
酵母エキス ５ｇ
ＮａＣｆ１ ５ ｇ
脱イオン水 １Ｑ
（ｐＨ７，０）
得られた培養液を濾過して、培養濾液とし、これを６Ｎ
ＨＣｆｌでｐＨ＝３．０とし、しばらく放置すると沈
澱物が得られるので、得られた沈澱物を蒸留水に溶解し
、これにＮａＨＣＯ，を加え、 ＰＨ＝７．０とし。Polypeptone 30g Yeast extract 5g NaCf1 5g Deionized water 1Q (pH 7,0) The obtained culture solution was filtered to obtain a culture filtrate, which was diluted with 6N
Adjust the pH to 3.0 with HCfl and leave it for a while to obtain a precipitate, so dissolve the obtained precipitate in distilled water, add NaHCO, and adjust the pH to 7.0.
更にエタノールを８０％まで加え、濾過する。得られた
上清を真空乾燥し、乾固物を得る。この乾固物を蒸留水
に溶解し、６Ｎ ＨＣＱでＰＨ＝３．０とし、しばらく
放置し、沈澱物を得る。この沈澱物を蒸留水に溶解し、
ＮａＨＣＯｉでＰＨ＝７．０とし、この溶液を蒸留水に
対して透析する。Further add ethanol to 80% and filter. The obtained supernatant is vacuum dried to obtain a dried product. This dried product is dissolved in distilled water, adjusted to pH=3.0 with 6N HCQ, and left to stand for a while to obtain a precipitate. Dissolve this precipitate in distilled water,
The pH is set to 7.0 with NaHCOi and the solution is dialyzed against distilled water.
得られた透析液を凍結乾燥し、物質ＫＢＳ３−Ｐ１００
４含有の粗精製物を黄色粉末で得る。The obtained dialysate was lyophilized and the substance KBS3-P100
A crude product containing 4 was obtained as a yellow powder.
得られた黄色粉末を高速液体クロマトにがけ、第３図に
矢印で示す物質ＫＢＳ３−Ｐ１００４該当部分をとり、
これを凍結乾燥することにより、はぼ純品の物質ＫＢＳ
３−Ｐ１００４を白色無定形粉末として得た。The obtained yellow powder was applied to high performance liquid chromatography, and the corresponding part of the substance KBS3-P1004 indicated by the arrow in Fig. 3 was taken.
By freeze-drying this, Habo pure substance KBS
3-P1004 was obtained as a white amorphous powder.
第１図は物質ＫＢＳ３−Ｐ４Ｏ１０の紫外線吸収スペク
トルを示す図で、第２図は同じく赤外線吸収スペクトル
を示す図で、第３図は物質ＫＢＳ３−Ｐ１００４含有粗
精製物の高速液体クロマトによる人２２０による吸光度
曲線を示す図である。矢印は物質ＫＢＳ３−Ｐ４Ｏ１０
を示す。Fig. 1 is a diagram showing the ultraviolet absorption spectrum of the substance KBS3-P4O10, Fig. 2 is a diagram showing the infrared absorption spectrum as well, and Fig. 3 is a diagram showing the infrared absorption spectrum of the substance KBS3-P1004, which was obtained by high performance liquid chromatography. FIG. 3 is a diagram showing an absorbance curve. The arrow indicates the substance KBS3-P4O10
shows.
Claims (2)
００４。 １、元素分析値 Ｃ：４８．６１％ Ｈ：６．６２％ Ｎ：１０．５４％ Ａｓｈ：１．４６％ ２、灰化点 １８０〜１８２℃ ３、物質の色及び性状：白色粉体 ４、紫外線吸収スペクトル：第１図に示す通り。 ５、赤外線吸収スペクトル：第２図に示す通り。 ６、溶剤に対する溶解性：水、メタノール、エタノール
、アセトニトリルに可溶。 ｎ−プロピルアルコール、アセトン、酢酸 エチル、エーテル、クロロホルム、ジエチ ルエーテル、石油エーテルに不溶。 ７、呈色反応 ニンヒドリンテスト ８、アミノ酸組成：アミノ酸分析機によるアミノ酸組成
は次の通りである。 ９、物質１分子あたり構造未決定の脂肪酸が１分子存在
する。(1) Substance KBS3-P1 with the following physical and chemical properties
004. 1. Elemental analysis value C: 48.61% H: 6.62% N: 10.54% Ash: 1.46% 2. Ashing point 180-182°C 3. Color and properties of substance: White powder 4 , UV absorption spectrum: as shown in FIG. 5. Infrared absorption spectrum: As shown in Figure 2. 6. Solubility in solvents: Soluble in water, methanol, ethanol, acetonitrile. Insoluble in n-propyl alcohol, acetone, ethyl acetate, ether, chloroform, diethyl ether, petroleum ether. 7. Color reaction ninhydrin test 8. Amino acid composition: The amino acid composition determined by an amino acid analyzer is as follows. 9. There is one molecule of undetermined fatty acid molecule per molecule of substance.
産菌を培養し、物質ＫＢＳ３−Ｐ１００４を採取するこ
とを特徴とする物質ＫＢＳ３−Ｐ１００４の製造法。(2) A method for producing the substance KBS3-P1004, which comprises culturing a substance KBS3-P1004-producing bacteria belonging to the genus Bacillus and collecting the substance KBS3-P1004.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61243236A JPS6399090A (en) | 1986-10-15 | 1986-10-15 | Kbs3-p1004 substance and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61243236A JPS6399090A (en) | 1986-10-15 | 1986-10-15 | Kbs3-p1004 substance and production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6399090A true JPS6399090A (en) | 1988-04-30 |
| JPH0378880B2 JPH0378880B2 (en) | 1991-12-17 |
Family
ID=17100861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61243236A Granted JPS6399090A (en) | 1986-10-15 | 1986-10-15 | Kbs3-p1004 substance and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6399090A (en) |
-
1986
- 1986-10-15 JP JP61243236A patent/JPS6399090A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0378880B2 (en) | 1991-12-17 |
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