JPS6399087A - Kbs2-p104 substance and production thereof - Google Patents
Kbs2-p104 substance and production thereofInfo
- Publication number
- JPS6399087A JPS6399087A JP61243233A JP24323386A JPS6399087A JP S6399087 A JPS6399087 A JP S6399087A JP 61243233 A JP61243233 A JP 61243233A JP 24323386 A JP24323386 A JP 24323386A JP S6399087 A JPS6399087 A JP S6399087A
- Authority
- JP
- Japan
- Prior art keywords
- substance
- kbs2
- ether
- amino acid
- ash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000126 substance Substances 0.000 title claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 title claims description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 21
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 9
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 9
- 150000001413 amino acids Chemical class 0.000 claims abstract description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000006243 chemical reaction Methods 0.000 claims abstract description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 3
- 238000000921 elemental analysis Methods 0.000 claims abstract description 3
- 239000000194 fatty acid Substances 0.000 claims abstract description 3
- 229930195729 fatty acid Natural products 0.000 claims abstract description 3
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 3
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000003208 petroleum Substances 0.000 claims abstract description 3
- 238000000862 absorption spectrum Methods 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 238000004380 ashing Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 2
- 101100163949 Caenorhabditis elegans asp-3 gene Proteins 0.000 abstract 1
- 101100315624 Caenorhabditis elegans tyr-1 gene Proteins 0.000 abstract 1
- 239000003429 antifungal agent Substances 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 description 9
- 239000012153 distilled water Substances 0.000 description 7
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 6
- 238000004128 high performance liquid chromatography Methods 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000000843 anti-fungal effect Effects 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000385 dialysis solution Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Landscapes
- Compounds Of Unknown Constitution (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は抗真菌活性を有する物質ＫＢＳ２−Ｐ１０４及
びその製造法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a substance KBS2-P104 having antifungal activity and a method for producing the same.
本発明者らは、バチルス属菌の生産する抗菌性低分子物
質を探索中バチルス属の一菌株が抗真菌活性物質を生産
することを知り、更に、該物質が新規物質であることを
確認し、これを物質ＫＢＳ２−Ｐ１０４と命名し、本発
明を完成するに到った。While searching for antibacterial low-molecular-weight substances produced by bacteria of the genus Bacillus, the present inventors discovered that a strain of the genus Bacillus produces an active antifungal substance, and further confirmed that the substance was a new substance. This was named substance KBS2-P104, and the present invention was completed.
本発明で使用する菌は物質ＫＢＳ２−Ｐ１０４を生産す
るものであればいずれでもよいが１例示としてはバチル
ス（Ｂａｃｉｌｌｕｓ） ＫＢ−５２があげられる。バ
チルスＫＢ−５２は微工研にＦＥＲＭ Ｐ−８９８５と
して寄託されており、また菌学的性質の概要は次の通り
である。The bacteria used in the present invention may be any species as long as it produces the substance KBS2-P104, and one example is Bacillus KB-52. Bacillus KB-52 has been deposited with the Microtech Institute as FERM P-8985, and the outline of its mycological properties is as follows.
ダラム染色 十
胞子染色 十
カタラーゼテスト ＋
オキシダーゼテスト ＋
物質ＫＢＳ２−Ｐ１０４生産菌の培養培地は、資化でき
る炭素源、窒素源、ビタミン等を含有する栄養料等適宜
含有したもので、物質ＫＢＳ２−Ｐ１０４を生産蓄積す
る培地であればいずれの培地でもよい。Durham staining Tenspore staining Ten catalase test + oxidase test + The culture medium for the substance KBS2-P104 producing bacteria contains appropriate nutrients such as assimilable carbon sources, nitrogen sources, vitamins, etc. Any medium may be used as long as it produces and accumulates.
バチルスＫＢ−５２の培養に適した培地は例えば次のも
のがあげられる。Examples of suitable media for culturing Bacillus KB-52 include the following.
ポリペプトン ３０ｇ 酵母エキス ５ｇ ＮａＣ１５ｇ 脱イオン水 ＩＱ。Polypeptone 30g Yeast extract 5g NaC15g Deionized water IQ.
（ＰＨ７，０）
培養は２５〜３０℃で、通気、撹拌、振どう等好気的に
４日間程度行なわれる。(PH 7,0) Cultivation is carried out aerobically for about 4 days at 25 to 30° C. with aeration, stirring, shaking, etc.
得られた培養液は濾過して濾液を得、これに６Ｎ ＨＣ
ｎを添加し、ｐＨ＝３．０に調整し沈澱物を得る。The obtained culture solution was filtered to obtain a filtrate, which was added with 6N HC.
Add n and adjust the pH to 3.0 to obtain a precipitate.
沈澱物は水に溶解し、ＮａＨＣＯ３を加え、ＰＨ＝７．
０とし、次いでエタノールを８０％まで加える。得られ
た上清液を真空乾燥し、乾燥物を蒸留水に溶解し、再び
６Ｎ ＨＣＱを加えてｐＨ＝３．０に調整し、沈澱物を
得る。得られた沈澱物を蒸留水に溶解し、ＮａＨＣＯ。The precipitate was dissolved in water and NaHCO3 was added to pH=7.
0 and then add ethanol to 80%. The obtained supernatant liquid is vacuum-dried, the dried product is dissolved in distilled water, and 6N HCQ is added again to adjust the pH to 3.0 to obtain a precipitate. The resulting precipitate was dissolved in distilled water and diluted with NaHCO.
を加え、ＰＨ＝７．０とし、蒸留水に対して透析する。was added, the pH was adjusted to 7.0, and the mixture was dialyzed against distilled water.
透析内液を凍結乾燥することによって物質ＫＢＳ２−Ｐ
１０４含有粗精製物を黄色粉末として得る。Substance KBS2-P was obtained by freeze-drying the dialysis fluid.
A crude product containing 104 is obtained as a yellow powder.
得られた黄色粉末を水に溶解し、アセトニトリル系で高
速液体クロマトにかけ、これを単離し、凍結乾燥するこ
とによってほぼ純品の物質ＫＢＳ２−Ｐ１０４を白色無
定形粉末として得る。The obtained yellow powder is dissolved in water, subjected to high performance liquid chromatography using an acetonitrile system, isolated and freeze-dried to obtain an almost pure substance KBS2-P104 as a white amorphous powder.
得られた物質ＫＢＳ２−Ｐ１０４の理化学的性質は次の
通りである。The physical and chemical properties of the obtained substance KBS2-P104 are as follows.
１、元素分析値 Ｃ：４９．２３％ Ｈ： ６．９８％ Ｎ ： １１．０８％ Ａｓｈ ： １．５６％ ２、灰化点 １８０〜１８２℃ ３、物質の色及び性状：白色粉体 ４、紫外線吸収スペクトル：第１図に示す通り。1. Elemental analysis value C: 49.23% H: 6.98% N: 11.08% Ash: 1.56% 2. Ashing point 180-182℃ 3. Color and properties of substance: white powder 4. Ultraviolet absorption spectrum: As shown in Figure 1.
５、赤外線吸収スペクトル：第２図に示す通り。5. Infrared absorption spectrum: As shown in Figure 2.
６、溶剤に対する溶解性：水、メタノール、エタノール
、アセトニトリルに可溶。6. Solubility in solvents: Soluble in water, methanol, ethanol, acetonitrile.
ｎ−プロピルアルコール、アセトン、酢酸エチル、エー
テル、クロロホルム、ジエチルエーテル、石油エーテル
に不溶。Insoluble in n-propyl alcohol, acetone, ethyl acetate, ether, chloroform, diethyl ether, petroleum ether.
７、呈色反応
ニンヒドリンテスト −
８、 アミノ酸組成ニアミノ酸分析機によるアミノ酸組
成は次の通りである。7. Color reaction ninhydrin test - 8. Amino acid composition The amino acid composition determined by an amino acid analyzer is as follows.
９、物質１分子あたり構造未決定の脂肪酸が１分子存在
する。9. There is one molecule of undetermined fatty acid molecule per molecule of substance.
１０、本物質は真菌に対して強い抗菌性を示す。10. This substance exhibits strong antibacterial properties against fungi.
１１、精製法：ｐＨ＝３．０程度で沈澱させ、沈澱物を
ＰＨ＝７．０で溶解する工程をくりかえし、最後に高速
液体クロマトにかけることによってほぼ純品を得ること
ができる。高速液体クロマトにおける人２２０による吸
光度曲線は第３図に示す通りである。矢印が物質ＫＢＳ
２−Ｐ１０４を示している。11. Purification method: An almost pure product can be obtained by repeating the steps of precipitating at about pH 3.0, dissolving the precipitate at pH 7.0, and finally applying high performance liquid chromatography. The absorbance curve of human 220 in high performance liquid chromatography is as shown in FIG. The arrow is the material KBS
2-P104 is shown.
なお、高速液体クロマトはカラムサイズφ１，０Ｘ２０
ａｎ、ゲルは日立ゲル３０５３ （シリカ系）、溶出条
件は初発アセトニトリル４０％、トリフルオロ酢酸０．
１％の水性溶媒で、最終（４０分後）アセトニトリル６
０％、トリフルオロ酢酸０．１％の水性溶媒になる直線
濃度勾配によって行った。In addition, for high performance liquid chromatography, the column size is φ1,0×20
An, the gel is Hitachi Gel 3053 (silica-based), the elution conditions are initial acetonitrile 40%, trifluoroacetic acid 0.
1% aqueous solvent, final (after 40 min) acetonitrile 6
A linear concentration gradient resulting in 0% trifluoroacetic acid and 0.1% trifluoroacetic acid in aqueous solvent was performed.
１２、抗菌スペクトラム：物質ＫＢＳ２−Ｐ１０４の抗
菌活性は次の表１に示される。12. Antibacterial spectrum: The antibacterial activity of substance KBS2-P104 is shown in Table 1 below.
次に本発明の実施例を示す。Next, examples of the present invention will be shown.
実施例１゜
バチルスＫＢ−３２，ＦＥＲＮ Ｐ−８９８５を次の培
地に接種して、２７℃で４日間振どう培養した。Example 1 Bacillus KB-32, FERN P-8985 was inoculated into the following medium and cultured with shaking at 27°C for 4 days.
ポリペプトン ３０ｇ
酵母エキス ５ｇ
ＮａＣｆ１ ５ ｇ
脱イオン水 ＩＱ
（ｐＨ７，０）
得られた培養液を濾過して、培養濾液とし、これを６Ｎ
ＩＩｃＱでｐＨ＝３．０とし、しばらく放置すると沈
澱物が得られるので、得られた沈澱物を蒸留水に溶解し
、これにＮａＨＣＯ，を加え、Ｐ）ｌ ＝ ７．０とし
、更にエタノールを８０％まで加え、濾過する。得られ
た上清を真空乾燥し、乾固物を得る。この乾固物を蒸留
水に溶解し、６Ｎ ＨＣＱでｐｌ（＝ ３．０とし、し
ばらく放置し、沈澱物を得る。この沈澱物を蒸留水に溶
解し、ＮａＨＣＯｓでＰＨ＝７．０とし、この溶液を蒸
留水に対して透析する。Polypeptone 30g Yeast extract 5g NaCf1 5g Deionized water IQ (pH 7,0) The obtained culture solution was filtered to obtain a culture filtrate, which was diluted with 6N
Adjust pH to 3.0 with IIcQ and leave for a while to obtain a precipitate. Dissolve the obtained precipitate in distilled water, add NaHCO to this to make P)l = 7.0, and add ethanol. Add to 80% and filter. The obtained supernatant is vacuum dried to obtain a dried product. Dissolve this dry solid in distilled water, adjust the pH to 3.0 with 6N HCQ, leave it for a while to obtain a precipitate. Dissolve this precipitate in distilled water, adjust the pH to 7.0 with NaHCOs, This solution is dialyzed against distilled water.
得られた透析液を凍結乾燥し、物質ＫＢＳ２−Ｐ１０４
含有の粗精製物を黄色粉末で得る。The resulting dialysate was lyophilized to form the substance KBS2-P104.
A crude product containing the compound was obtained as a yellow powder.
得られた黄色粉末を高速液体クロマトにかけ、第３図に
矢印で示す物質ＫＢＳ２−Ｐ１０４該当部分をとり、こ
れを凍結乾燥することにより、はぼ純品の物質ＫＢＳ２
−Ｐ１０４を白色無定形粉末として得た。The obtained yellow powder was subjected to high-performance liquid chromatography, and the corresponding part of the substance KBS2-P104 indicated by the arrow in Fig. 3 was taken, and this was freeze-dried to obtain the pure substance KBS2.
-P104 was obtained as a white amorphous powder.
第１図は物質ＫＢＳ２−Ｐ１０４の紫外線吸収スペクト
ルを示す図で、第２図は同じく赤外線吸収スペクトルを
示す図で、第３図は物質ＫＢＳ２−Ｐ１０４含有粗精製
物の高速液体クロマトによる人２２０による吸光度曲線
を示す図である。矢印は物質ＫＢＳ２−Ｐ１０４を示す
。Figure 1 is a diagram showing the ultraviolet absorption spectrum of the substance KBS2-P104, Figure 2 is a diagram showing the infrared absorption spectrum, and Figure 3 is a diagram showing the infrared absorption spectrum of the substance KBS2-P104, which was obtained by high-performance liquid chromatography. FIG. 3 is a diagram showing an absorbance curve. The arrow indicates substance KBS2-P104.
Claims (2)
０４。 １、元素分析値 Ｃ：４９．２３％ Ｈ：６．９８％ Ｎ：１１．０８％ Ａｓｈ：１．５６％ ２、灰化点 １８０〜１８２℃ ３、物質の色及び性状：白色粉体 ４、紫外線吸収スペクトル：第１図に示す通り。 ５、赤外線吸収スペクトル：第２図に示す通り。 ６、溶剤に対する溶解性：水、メタノール、エタノール
、アセトニトリルに可溶。 ｎ−プロピルアルコール、アセトン、酢酸 エチル、エーテル、クロロホルム、ジエチ ルエーテル、石油エーテルに不溶。 ７、呈色反応 ニンヒドリンテスト ８、アミノ酸組成：アミノ酸分析機によるアミノ酸組成
は次の通りである。 ９、物質１分子あたり構造未決定の脂肪酸が１分子存在
する。(1) Substance KBS2-P1 with the following physical and chemical properties
04. 1. Elemental analysis value C: 49.23% H: 6.98% N: 11.08% Ash: 1.56% 2. Ashing point 180-182°C 3. Color and properties of substance: White powder 4 , UV absorption spectrum: as shown in FIG. 5. Infrared absorption spectrum: As shown in Figure 2. 6. Solubility in solvents: Soluble in water, methanol, ethanol, acetonitrile. Insoluble in n-propyl alcohol, acetone, ethyl acetate, ether, chloroform, diethyl ether, petroleum ether. 7. Color reaction ninhydrin test 8. Amino acid composition: The amino acid composition determined by an amino acid analyzer is as follows. 9. There is one molecule of undetermined fatty acid molecule per molecule of substance.
菌を培養し、物質ＫＢＳ２−Ｐ１０４を採取することを
特徴とする物質ＫＢＳ２−Ｐ１０４の製造法。(2) A method for producing the substance KBS2-P104, which comprises culturing a substance KBS2-P104-producing bacteria belonging to the genus Bacillus and collecting the substance KBS2-P104.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61243233A JPS6399087A (en) | 1986-10-15 | 1986-10-15 | Kbs2-p104 substance and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61243233A JPS6399087A (en) | 1986-10-15 | 1986-10-15 | Kbs2-p104 substance and production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6399087A true JPS6399087A (en) | 1988-04-30 |
| JPH0378877B2 JPH0378877B2 (en) | 1991-12-17 |
Family
ID=17100815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61243233A Granted JPS6399087A (en) | 1986-10-15 | 1986-10-15 | Kbs2-p104 substance and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6399087A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0601187A1 (en) * | 1992-03-31 | 1994-06-15 | Higeta Shoyu Co., Ltd. | Novel antibiotic and production and use thereof |
-
1986
- 1986-10-15 JP JP61243233A patent/JPS6399087A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0601187A1 (en) * | 1992-03-31 | 1994-06-15 | Higeta Shoyu Co., Ltd. | Novel antibiotic and production and use thereof |
| EP0601187A4 (en) * | 1992-03-31 | 1994-09-21 | Higeta Shoyu Kk | Novel antibiotic and production and use thereof. |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0378877B2 (en) | 1991-12-17 |
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