JPS6366950B2 - - Google Patents

Description

[Detailed description of the invention] [Industrial application field] The present invention relates to a method for treating rugs and blankets.

[Conventional technology]

Causative substances (antigens) of allergic diseases such as allergic asthma, allergic rhinitis, and conjunctivitis
Pollen, house dust, etc. are known as mites, but recently the feces, corpses, and living bodies of mites that live indoors have become more prominent, and dust mites are the most prominent among mites. It is said.

Generally, mites are removed from rugs, blankets, etc. by using insecticides, vacuuming, sunbathing, or hitting them, but it has not been possible to eradicate them.

[Problem that the invention seeks to solve]

Although biological allergic reactions caused by dust mites are medically considered to belong to type 1 allergies (immediate type), we have discovered that some dust mite allergies belong to type 4 allergies (delayed type).

There was a problem that dust mites caused allergies.

[Means for solving problems]

The present invention aims to solve the above-mentioned problems by:
It is characterized in that allergic reactions can be prevented by treating carpets, blankets, etc. with a 5% to 5% tannic acid aqueous solution.

Example 1 One milligram of the antigen extract of the house dust mite was placed in a 20 milliliter flask, and one milligram of tannic acid was added.
% aqueous solution and left to stand at room temperature for 2 hours to obtain a tannic acid-treated solution of house dust mite antigen. Similarly, 1 milligram of the dust mite antigen extract is sprinkled with 10 milliliters of pure water and left to stand for 2 hours to obtain an untreated solution of the dust mite antigen.

An amount equivalent to 20 to 400 micrograms of pure dust mite antigen extract per mouse was intraperitoneally administered to experimental mice and guinea pigs that had been immunized in advance with house dust mite antigen extract and an immune enhancer (cyclophosphamide). When the treated and untreated solutions were injected and the allergic effects of both solutions were compared, the untreated solution caused a skin reaction on the soles of the feet of mice after 5 days, and on the flanks of guinea pigs after 6 to 14 days. However, in the case of the treated solution, similar skin reactions occurred, but the symptoms were 1/2 to 1/3 as mild as in the case of the untreated solution.

Injecting the supernatant liquid of the treatment solution had a remarkable effect in that the symptoms did not occur at all.

No matter how many times these tests were carried out, similar results were obtained.

Example 2 Living organisms of mites, of which dust mites account for 90% or more,
Divide 10 square meters of carpet in a living residence where there are many dead bodies and feces into two sections. Spray each section with a 1% tannic acid aqueous solution at a rate of 60 milliliters per square meter, and let it air dry to make it clean. I used a vacuum cleaner to collect dust. 0.1 g of this dust was dispersed in 100 ml of pure water, and a liquid was obtained by removing impurities with a filter. Two milliliters of an emulsion containing cyclophosphamide, which is known to increase delayed allergic symptoms, at a ratio of 100 milligrams per milliliter of dust solution, was injected intraperitoneally into the immunized mice. 2 out of 10 mice died
No skin reactions were observed on the soles of the feet in the remaining eight animals, including this one. Meanwhile, spray 60 milliliters of pure water per square meter on the remaining section of the carpet and let it dry naturally.
In mice, 2 milliliters of the emulsion obtained from the dust was injected intraperitoneally using the same procedure as above.
Strong skin reactions on the soles of the feet were observed in the remaining seven animals, including the three that died.

Example 3 4.5 square meters of carpet containing dust mites, of which 93% were dust mites, was divided in half, and a carpet rinse machine (containing water in tank A, spraying this water from an adapter onto the carpet, and immediately Rinse the carpet with a 5% aqueous solution of tannic acid using a machine that rinses the carpet by absorbing it from the adapter into tank B. Filter the waste water in tank B, add cyclophosphamide, and boil for 10 minutes. The mite experimental solution obtained using the mite test solution produced only a very slight type 4 allergic reaction in guinea pigs with a dust mite allergy. Depending on the fluid, severe type 4 allergy symptoms were observed in the flank.

Example 4 Cut a blanket in which dust mites are breeding in half 3
% tannic acid aqueous solution in an electric washing machine, and the washing liquid was collected. The remaining half of the blanket was washed with tap water that did not contain tannic acid, and this washing solution was also collected. Pour 2 liters of each of these washing solutions into separate large beakers and heat gently to approx.
This was boiled down to 200 milliliters, passed through a suction buchner to remove impurities, and the resulting solution was used as an allergy experiment solution. When 2 milliliters of this was injected into the abdominal cavity of a mouse with a dust mite allergy, tannic acid was detected. In the system, a slight reaction occurred on the soles of the feet, but in the tap water system, the same reaction was 2 to 3 times more severe.

〔Effect of the invention〕

As mentioned above, the present invention can minimize or eliminate allergic reactions caused by mites by a simple method of treating carpets, blankets, etc. with an aqueous tannic acid solution, while causing no effect on the human body. There are some products that have remarkable hygienic effects that do not give any harmful effects.

Claims (1)

[Claims] 1. A method for treating rugs and blankets, which comprises treating with an aqueous solution of tannic acid to remove or attenuate the antigenicity of an antigen brought about by house dust mites.