JPS6350748A - 酵素電極システム - Google Patents
酵素電極システムInfo
- Publication number
- JPS6350748A JPS6350748A JP61196193A JP19619386A JPS6350748A JP S6350748 A JPS6350748 A JP S6350748A JP 61196193 A JP61196193 A JP 61196193A JP 19619386 A JP19619386 A JP 19619386A JP S6350748 A JPS6350748 A JP S6350748A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- hydrogen peroxide
- enzyme
- electrolytic
- immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 63
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 63
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 90
- 238000001514 detection method Methods 0.000 claims abstract description 14
- 229920005597 polymer membrane Polymers 0.000 claims abstract description 11
- 229920000642 polymer Polymers 0.000 claims description 13
- 239000010409 thin film Substances 0.000 claims description 12
- 229920006254 polymer film Polymers 0.000 claims description 6
- 239000000758 substrate Substances 0.000 abstract description 18
- 238000006116 polymerization reaction Methods 0.000 abstract description 13
- 230000004044 response Effects 0.000 abstract description 7
- 229940088598 enzyme Drugs 0.000 description 54
- 238000000034 method Methods 0.000 description 19
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 229910052760 oxygen Inorganic materials 0.000 description 12
- 239000001301 oxygen Substances 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 239000012528 membrane Substances 0.000 description 11
- 238000005259 measurement Methods 0.000 description 10
- 239000012085 test solution Substances 0.000 description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 108010015776 Glucose oxidase Proteins 0.000 description 7
- 239000004366 Glucose oxidase Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 229940116332 glucose oxidase Drugs 0.000 description 7
- 235000019420 glucose oxidase Nutrition 0.000 description 7
- 229910052697 platinum Inorganic materials 0.000 description 7
- 239000000178 monomer Substances 0.000 description 5
- 230000004043 responsiveness Effects 0.000 description 5
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 4
- 238000005868 electrolysis reaction Methods 0.000 description 4
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008151 electrolyte solution Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 description 2
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 description 2
- 239000003125 aqueous solvent Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- 108010025188 Alcohol oxidase Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 108010000659 Choline oxidase Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108010015133 Galactose oxidase Proteins 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022624 Glucoamylase Human genes 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 108010073450 Lactate 2-monooxygenase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 102000010909 Monoamine Oxidase Human genes 0.000 description 1
- 108010062431 Monoamine oxidase Proteins 0.000 description 1
- 101100518501 Mus musculus Spp1 gene Proteins 0.000 description 1
- 108010063734 Oxalate oxidase Proteins 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 1
- 239000002262 Schiff base Substances 0.000 description 1
- 150000004753 Schiff bases Chemical class 0.000 description 1
- 108010055297 Sterol Esterase Proteins 0.000 description 1
- 102000000019 Sterol Esterase Human genes 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 108010092464 Urate Oxidase Proteins 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 102000020006 aldose 1-epimerase Human genes 0.000 description 1
- 108091022872 aldose 1-epimerase Proteins 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 102000016679 alpha-Glucosidases Human genes 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- -1 and for example Substances 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229940075397 calomel Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- GTKRFUAGOKINCA-UHFFFAOYSA-M chlorosilver;silver Chemical compound [Ag].[Ag]Cl GTKRFUAGOKINCA-UHFFFAOYSA-M 0.000 description 1
- 150000001840 cholesterol esters Chemical class 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 1
- 230000005518 electrochemistry Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
- 229940107700 pyruvic acid Drugs 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229930192474 thiophene Natural products 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61196193A JPS6350748A (ja) | 1986-08-20 | 1986-08-20 | 酵素電極システム |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61196193A JPS6350748A (ja) | 1986-08-20 | 1986-08-20 | 酵素電極システム |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6350748A true JPS6350748A (ja) | 1988-03-03 |
| JPH0529062B2 JPH0529062B2 (enrdf_load_stackoverflow) | 1993-04-28 |
Family
ID=16353740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61196193A Granted JPS6350748A (ja) | 1986-08-20 | 1986-08-20 | 酵素電極システム |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6350748A (enrdf_load_stackoverflow) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6428555A (en) * | 1987-07-24 | 1989-01-31 | Terumo Corp | Enzyme sensor |
| JPS6432160A (en) * | 1987-07-29 | 1989-02-02 | Terumo Corp | Enzyme sensor and production thereof |
| US5683562A (en) * | 1994-09-14 | 1997-11-04 | Avl Medical Instruments Ag | Planar sensor for determining a chemical parameter of a sample |
| JP2008256725A (ja) * | 2001-05-31 | 2008-10-23 | Instrumentation Lab Co | 分析装置およびバイオセンサ、ならびにこれらの精度および有効期間を増大させるための方法 |
| JP2011242385A (ja) * | 2010-04-22 | 2011-12-01 | Arkray Inc | バイオセンサ |
| JP2012237655A (ja) * | 2011-05-12 | 2012-12-06 | Hioki Ee Corp | 微量成分検出装置および微量成分検出方法 |
| WO2014002999A1 (ja) | 2012-06-25 | 2014-01-03 | 合同会社バイオエンジニアリング研究所 | 酵素電極 |
| CN111398386A (zh) * | 2020-05-12 | 2020-07-10 | 山东省科学院生物研究所 | 一种固定化酶电极、固定化酶传感器及其酶膜抗干扰的检测方法 |
-
1986
- 1986-08-20 JP JP61196193A patent/JPS6350748A/ja active Granted
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6428555A (en) * | 1987-07-24 | 1989-01-31 | Terumo Corp | Enzyme sensor |
| JPS6432160A (en) * | 1987-07-29 | 1989-02-02 | Terumo Corp | Enzyme sensor and production thereof |
| US5683562A (en) * | 1994-09-14 | 1997-11-04 | Avl Medical Instruments Ag | Planar sensor for determining a chemical parameter of a sample |
| JP2008256725A (ja) * | 2001-05-31 | 2008-10-23 | Instrumentation Lab Co | 分析装置およびバイオセンサ、ならびにこれらの精度および有効期間を増大させるための方法 |
| JP2011242385A (ja) * | 2010-04-22 | 2011-12-01 | Arkray Inc | バイオセンサ |
| JP2012237655A (ja) * | 2011-05-12 | 2012-12-06 | Hioki Ee Corp | 微量成分検出装置および微量成分検出方法 |
| WO2014002999A1 (ja) | 2012-06-25 | 2014-01-03 | 合同会社バイオエンジニアリング研究所 | 酵素電極 |
| CN111398386A (zh) * | 2020-05-12 | 2020-07-10 | 山东省科学院生物研究所 | 一种固定化酶电极、固定化酶传感器及其酶膜抗干扰的检测方法 |
| WO2021227542A1 (zh) * | 2020-05-12 | 2021-11-18 | 山东省科学院生物研究所 | 一种固定化酶电极、固定化酶传感器及其酶膜抗干扰的检测方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0529062B2 (enrdf_load_stackoverflow) | 1993-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |