JPS635036A - Squalene-containing composition - Google Patents

Abstract

PURPOSE:To obtain a squalene-containing composition useful as a nutrient assistant and pharmaceutical, etc., by adding a phospholipid to squalene, thereby stabilizing squalene. CONSTITUTION:Squalene is a compound having the chain structure of formula, high impregnation property and activation activity and exhibiting effective physiological action to gastric ulcer and hepatic diseases, however, it has poor stability and is liable to be easily decomposed with oxygen or heat owing to its highly unsaturated structure. The objective stabilized squalene-containing composition can be produced by compounding (A) 100pts.wt. of squalene with (B) preferably about >=1pt., especially about >=3pts.wt. of one or more kinds of phospholipids selected from phosphatidylcholine, phosphatidyl ethanolamine, sphingomyelin, etc., as a stabilizer for the component A and preferably further (C) preferably 1-10pts.wt. of an antioxidant, especially a natural antioxidant such as vegetable oil containing tocopherol.

Description

[Detailed description of the invention] [Industrial application field] The present invention relates to stable squalene-containing compositions.

[Prior art/problems to be solved by the invention] Squalene is a compound with a chain structure of 30 carbon atoms consisting of 6 isoprene residues, and is a colorless oily substance found in shark liver oil. It is also called GiWj shark extract because it is extracted from deep-sea sharks.

In recent years, squalene was discovered in 1916 and has been mainly used as a cosmetic raw material, but in 1974,
It has come to be considered a dietary supplement.

Examples of the physiological effects of squalene include the following.

(1) Rich in permeability and activating effect, effective against diseases such as gastric ulcers; (2) Effective against liver diseases (this is thought to be a substance that sends oxygen to diseased cells and gives them activation) (3) Squalene has the function of transporting oxygen to cells and gives them vitality, but as it is a highly unsaturated compound, it has poor stability and is easily degraded by oxygen, heat, etc. is considered to be a problem.

It is therefore an object of the present invention to provide stable compositions of squalene.

[Means for solving problems]

The present inventor has conducted various studies on the stabilization of squalene, and has discovered that phospholipids have a stabilizing effect on squalene, and has completed the present invention.

That is, the present invention relates to a squalene-containing composition characterized in that it contains at least one type selected from phospholipids as a squalene stabilizer, and the present invention also relates to a squalene-containing composition that is characterized by containing at least one type selected from phospholipids as a squalene stabilizer. The present invention relates to a squalene-containing composition containing an oxidizing agent.

Squalene has the following structural formula consisting of 6 isoprene residues. It has a chain structure represented by .

It is desirable to use squalene of high purity, and it is contained in the composition in an amount of 20% by weight or more, preferably 30% by weight or more.

The phospholipid used as a stabilizer in the present invention is a general term for lipids containing phosphorus, and there are those that have a basic skeleton of glyceride and those that do not, and that have one to two fatty acid residues.
I have one. Examples include phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin. The phospholipid used in the present invention may be used not only as the above-mentioned phospholipid alone, but also in the form of a mixture of the above-mentioned phospholipids, or a product containing the above-mentioned phospholipids. In particular, it is preferable to use lecithin (eg, soybean lecithin, egg yolk lecithin), hydrogenated lecithin, and the like.

In the composition of the present invention, the phospholipid (for example, lecithin) is about 1 part by weight or more per 100 parts by weight of squalene.
In particular, it is preferable to add about 3 parts by weight or more.

The present invention provides a composition further containing an antioxidant. The antioxidant is preferably a natural antioxidant, such as vegetable oils such as tocopherols, flavone derivatives, gallic acid derivatives, catechic acid, sterols, terpenes, or plant extracts containing these. Examples of plants containing tocopherols include wheat germ, cottonseed, soybeans, etc.; examples of plants containing flavone derivatives include those belonging to the genus Primrose, digitalis, licorice, etc.; examples of plants containing gallic acid derivatives include pentagrams, galls, etc. Examples of catechic acid-containing plants include trees of the genus Eucalyptus and Ursica, and examples of sterol-containing plants include rapeseed, yeast, and soybean.

It is desirable that the antioxidant be blended in an amount of 1 to 10 parts by weight per 100 parts by weight of squalene.

In addition to phospholipids and antioxidants, the composition of the present invention may further contain a substance that dissolves phospholipids, particularly a substance that dissolves phospholipids and squalene. As the solubilizing agent, vegetable oils such as tocopherols, which are antioxidants mentioned above, and citrus extracts containing terpenes may also be used as solubilizing agents. oil, etc.), organic solvent, protein decomposition product (collagen hydrolyzate, etc., preferably with a molecular weight of 1000 to 3000)
The amount of the substance may be one or more sufficient to dissolve squalene and phospholipid.

Stabilization of squalene is promoted by further blending phosphoric acid, citric acid, ascorbic acid, melanoidin, nucleic acid, feral acid ester, etc. into the composition of the present invention.

The composition of the present invention is used as a food, a nutritional supplement, a pharmaceutical, and the like. Such products include, for example, beeswax,
It can be prepared by conventional means by blending lactose and the like.

[Example/Experiment example]

Examples 1 to 5 The compositions shown in Table 1 below were mixed until homogeneous to produce squalene-containing compositions. Note that the numbers in the table indicate parts by weight.

(Margin below) Table 1 Comparative example 1 A single squalene formulation was prepared.

Comparative Example 2 A composition consisting of 100 parts by weight of squalene and 2 parts by weight of vitamin E was prepared.

Experimental Example 1 Using the formulations of Examples 1 to 5 and Comparative Example 1.2, 60
After leaving it uncovered in a constant temperature water bath at ℃, a portion of it was sampled at regular intervals, the peroxide contained therein was quantified, and the peroxide value was determined. The results are shown in Table 2. Ta. Note that the numbers in parentheses in the table indicate the change in peroxide value at each measurement time, with the value at 0 hours being O.

Method for quantifying peroxide and determining peroxide value: Sample 1
0 g was properly collected in an Erlenmeyer flask with a co-sensor, 60 - of a solvent (chloroform: 2: glacial acetic acid: 3) was added, and the mixture was gently shaken to dissolve it into a transparent solution.

Add saturated potassium iodide solution in/and immediately mix for 1 minute, add 60I117 of water, and titrate with 0.0IN sodium thiosulfate solution using starch solution as an indicator. The end point is when the coloring caused by starch disappears. A blank test will be conducted in parallel.

The peroxide value is determined by the following formula.

Peroxide value (■equivalent/kg) = (A-B)XFX10+
CA-Amount of sodium thiosulfate solution used in this test (a/)
B = Amount of sodium thiosulfate solution used in blank test (-) C =
Amount of sample collected (g) F = Potency of sodium thiosulfate solution (margin) [Effect] Phospholipid has excellent stability against squalene. Therefore, in compositions in which phospholipids are added to squalene, is the squalene contained therein cheap? exists in

Shinihaku

Claims (2)

[Claims] (1) A squalene-containing composition comprising at least one selected from phospholipids as a squalene stabilizer. (2) The composition according to claim (1), further comprising an antioxidant.