JPS6349986B2 - - Google Patents

Description

[Detailed description of the invention]

[A] Object of the Invention The present invention relates to a novel application of saccharides extracted from the roots or the bulk of the roots of Barley mondo. Mugimondo is a Chinese medicine name, and its botanical name (base) refers to the roots of the Liliaceae family, such as Jyanohige and Yaburan, as shown in the following table (Table 1), or the ampulla of these roots. That's true.

【table】

[Table] (Industrial Application Field) The barley mondo extracted saccharide according to the present invention has a molecular weight of
It is in the range of 500 to 10,000, and even if administered orally, most of it is not broken down in the stomach or intestines, so it is used as a low-calorie food for obese people with excessive fat content (obese people), or as a health food as a weight loss agent. It can be used. Bakumonfuyu extracted saccharide according to the present invention (hereinafter, for convenience,
(simply abbreviated as barley mondo sugar) is characterized by its excellent slimming effect on obese people as described above, as well as its antitussive effect and is effective in stopping all kinds of coughs. In addition, it can also be used as a growth agent for intestinal bifidobacteria, as a poultice, as a thickener for foods or cosmetics, or as a moisturizing agent, excipient (bulking agent), or as a filler for pharmaceuticals, foods, cosmetics, etc. It can also be used as a coating agent for powder, granules, and tablets during processing. The barley hilt sugar according to the present invention has no oral toxicity, is almost tasteless and odorless, and can be obtained as a white powder without bitterness.
Therefore, it can be administered (orally) to a wide range of people from infants to adults. It is also effective for improving people with weak constitutions such as those with childhood asthma, or those with food allergies, and is not only a health food.
It can also be used in the field of pharmaceutical therapy. The barley mondo sugar according to the present invention is as described above.
It consists of oligosaccharides to polysaccharides with a molecular weight of 500 to 10,000, and the total sugar content is 95 to 98% or more, but it contains savonins and flavonoids, which have traditionally been known as the main components of barley. is characterized in that it is not included. (Prior Art) To date, there have been no reports other than the present invention of the use of barley portal sugar for obesity. On the other hand, in Chinese medicine, Bakumondoto is used to suppress coughs and remove phlegm. For example, Bakumondoto is used to treat intense coughs that seem to be rising up, and phlegm that is very sticky and difficult to get out. However, until now, it was unknown what kind of active ingredient it is. It has been reported that the main components contained in Bakumonto include steroid glycosides, homoisoflavonoids, and oligosaccharides (see later publications). However, no literature has been found that states that saccharides obtained from barley mondo according to the present invention are used for symptoms associated with coughs and sputum. (Publication location) (1) Hisakola Kato et al.: Pharmaceutical Journal Vol. 88, p. 710 (1968) (2) Shoji J. et al.: Chemical Pharmaceutical Bulletin (Shoji, J., etal: Chem.
Pharm.Bull.) Vol.20, p.1729 (1972) Vol.21, p.308 (1973) Vol.25, p.3049 (1977) (3) Tada A et al. A., etal: Chem.Pharm.Bull.) Vol. 28, p. 1477 (1980) p. 2039 ( 〃 ) p. 2487 ( 〃 ) (4) Masashi Tomoda et al.: Vol. 16, p. 113 (1968) Vol. 25, p. 659 (1973) (5) Shoji Tomoda et al.: Journal of Herbal Medicine Vol. 20, p. 12 (1966) Recent improvements in dietary habits have resulted in overnutrition. Low calorie foods and low sugar foods (which cannot be converted into energy within the body) are desired. Among them,
Deficiency of vegetables containing high-molecular polysaccharide fiber is caused by
It is estimated that this is causing an increase in obesity and allergic diseases in children, as well as triggering diabetes and arteriosclerosis. However, if large amounts of plant fiber are given, it will put a strain on the stomach and intestines, resulting in indigestion and abdominal pain.
Furthermore, the higher the water-insoluble polymeric polysaccharide fiber, the higher this tendency. Therefore, water-soluble small saccharides and polysaccharides are desired. As a typical example, the use of glucomannan is known. However, when it comes to reducing weight in obese people, it is not very effective, and there has been a desire for a sugar substitute. In other words, the known use of glucomannan takes advantage of its property of swelling and expanding many times in the presence of water such as stomach and intestinal fluid, thereby giving a feeling of fullness and suppressing food intake. to do. In other words, people try to lose weight by restricting their daily diet, and even if weight loss is possible temporarily, if it continues, malnutrition and other side effects may occur. It is known to cause (Problems to be Solved by the Invention) The present inventor has discovered that the present invention does not cause abdominal distension even when taken, does not serve as a nutritional source, and is easily excreted in the defecation (excreted from the body) in an undigested state. In search of polysaccharides, we began our research by attempting to extract polysaccharides that could more easily achieve our goals using various plants as raw materials. Conventional ingredients that have a slimming effect when administered orally include glucomannan, as mentioned above, and recently, saponins.
These drawbacks are as mentioned above for gluconanman, and for saponins, a considerable amount is not required to achieve this, oral toxicity or pharmacological activity is strong, and it is not suitable for health foods. As such, there are many disadvantages. In other words, as a health food, it is desirable that it has no side effects and is highly safe.In other words, it is desirable that it not show any side effects even if you eat the amount equivalent to one meal at a time. It is. When it comes to meeting this condition, for example, known glucomannan is unsuitable due to its expansive nature and the need for a large amount of water. In other words, glucomannan is
The amount of water required at one time is approximately 30-50g, but the amount of water required for this is at least 300-50g.
500ml is required, and unless you replenish this amount of water, your body will not be able to maintain its normal state. In other words, if you ingest more water at once, you will need to drink the equivalent amount of water, and as a result, you will need to exercise (such as sports) to burn off the water you have ingested. If you continue to take the drug without this, you will have the disadvantage of inducing diarrhea and constipation, which will make it impossible to lose weight. Therefore, the amount of glucomannan used for the purpose of weight loss is around 3 to 5 g per day, taking into account side effects, and products that are taken daily are the mainstream. However, its effects were not fully demonstrated. As an alternative to this, the use of mainly vectors found in various fruits is progressing.
A typical example of this is the use of polysaccharides in prunes; products derived from these fruits are said to have no side effects, do not require large amounts of water, and are excellent in weight loss effects. ing. The present inventors understood the current situation and searched for oligosaccharides and polysaccharides that can be easily used as health foods, are low-calorie foods, and have excellent weight loss effects. One of the key points that satisfies this condition and allows weight loss to be achieved without side effects is that it does not require large amounts of water and is easy to take. Hattori:Do not give a feeling of bloating. Easily soluble in water. Furthermore, as a condition for weight loss without side effects, the most important point is that the molecular weight of various oligosaccharides and polysaccharides is 500%.
I've found that something in the ~10000 range is best. That is, in the present invention, the molecular weight is limited to barley portal sugar and is defined in the range of 500 to 10,000, but this point will be useful in the future application of sugars in low-calorie foods. Not only barley sugar, but also various oligosaccharides and polysaccharides derived from other plants, within this range are recommended to achieve the best weight loss effect without putting a burden on the stomach or intestines. , this could be one means. [B] Structure of the Invention The present invention consists of using saccharides extracted from barley mondo with a molecular weight in the range of 500 to 10,000 for low-calorie foods. Specific means will be shown below using Examples. (Means for solving the problem) Example 1 20 times the amount of water is added to 1 kg of Mugimonfu, heated and extracted, and then filtered to obtain a filtrate. Celite (Radiolite No. 200, Radiolite No. 600, etc.) is added to this filtrate and filtered to obtain a filtrate, which is then sequentially passed through alumina, cation exchange resin, anion exchange resin, and activated carbon. Leave the obtained liquid as it is, or
Or, after diluting up to 4 times (adding water), pass it through a Pellicon membrane with a molecular weight of 10,000 using a Pellicon cassette system (manufactured by Nippon Millipore Limited).
Furthermore, the eluate passing through the Pellicon membrane with a molecular weight of 1000 is removed, and the resulting solution (molecular weight 10,000 to 1000) is dried to obtain a white powder. Its total yield is 700 ~
You can get about 900g. When quantifying this,
Total sugar content is 98% or more. Example 2 40 times the amount of water was added to 1 kg of Mugimonfu, heated and extracted, and filtered through Celite to obtain the filtrate. Activated carbon was added to the filtrate, stirred, and filtered to obtain a clear solution. Dry the liquid to obtain a milky white powder. or,
The yield is 700-900g. When quantified, this product has a total sugar content of 95% or more. Example 3 Add 20 times the amount of water to 1 kg of Mugimonfu, heat extraction and celite filtration to obtain the filtrate, concentrate this filtrate to 1/2 to 1/5 volume, The concentrate is filtered again to obtain a clear viscous liquid. Yield is 800
It is around g. When this product is further made into a dry powder, its total sugar content is 95% or more. Example 4 Add 20 times the amount of water to 1 kg of barley Mondou, heat and extract, filter through Celite, then treat the filtrate with activated carbon, and concentrate to reduce the sugar content to 30-70%. Obtain a clear and viscous liquid. Its yield is 800 ~
It is 2000g. According to the extraction method shown in the above-mentioned Examples, the medicinal components contained in Mumondo, such as saponins and isoflavonoids, are removed, and the resulting pharmacological activity and oral toxicity are not exhibited. In addition, the obtained extract is the target barley portal sugar, but the molecular weight distribution is as shown in Figure 1, ranging from 500 to 10,000.
It is composed of sugars with a molecular weight range of . In addition, in each Example, regarding the starting material, barley mondo, there was no particular limitation on the plant base, but as shown in the previous table (Table 1), extraction can be performed using any of the barley mondo. It is. In other words, the Japanese Bureau stipulates the bulk of the root of the jianohige.
However, most of the barley monfu used in traditional Chinese medicine prescriptions in Japan are easily available.
Most of them are the roots of Chinese herbaceous or jianohige, or the bulk of these roots. Therefore, extraction was attempted using the roots and bulk of the roots of both plants, but there was no difference between the two plants in terms of yield. Therefore, it was thought that there was no need to be particular about the plant origins of Mugimonfuyu. The measurement conditions for the molecular weight distribution in FIG. 1 are as follows. Equipment: High Performance Liquid Chromatography (TWINCLE) Detector: RI (Shimazu RID-2A) Column: Finepak SIL AF-102 Temperature: 40℃ Mobile phase: 0.1M KH ₂ PO, 0.01M NaHPO ₄ 0.2M Na ₂ SO ₄ aqueous solution flow rate: 1 ml/min On the other hand, the barley montage obtained in the examples is characterized by having almost no gel-forming ability, although it dissolves easily in water. In other words, conventional polysaccharides such as glucomannan and pectins all have a high gel-forming ability and have the property of expanding like an agar when they contain water.
After ingestion, there is no space left for other meals, resulting in a feeling of fullness in the stomach and intestines, which is one of the conditions that can be used as a means to achieve weight loss. [C] Effects of the Invention The barley hilt sugar according to the present invention has good solubility in water and does not form a gel, so it has excellent formulation (compounding) properties and can be used in the form of a drink by adding it in large quantities. I can do it. Furthermore, after ingestion (dose), it has no gel-forming ability, rarely gives a feeling of fullness, and is difficult to be digested and broken down in the stomach and intestines. Therefore,
It is ideal as a low calorie food. (Confirmation of action or effect) The following table (Table 1) shows a method of taking 3 to 10 g before meals in the morning, noon, and evening every day, based on obese volunteers who did not immediately reduce their diet. Instructed to take (drink) 2 kg in 60 days.
Weight gain/loss was measured every other week, and the results are shown. Furthermore, after the completion of the 2-month dosing period and 30 days after discontinuation of administration, the weight was measured and investigated. In addition, in Table 1, body weight was measured every 6th day.
It was measured after the first urination in the morning. On the other hand, using the same administration (ingestion) method, we selected volunteers with approximately normal body weight and examined the changes in body weight during the administration period. Although the drug showed a weight loss effect, no particular reduction in body weight was observed in those who maintained a normal body weight. Therefore, barley mondo sugar appears to be selectively effective only in obese individuals.
In addition, there were no complaints of side effects during both tests. In addition, although none of the patients complained of diarrhea or constipation, many of them reported having easy stools and intestines, which led to the suspicion that the drug contained an intestinal regulating agent or digestive agent. There have been reports of an increase in

[Table] Judging from the performance results shown in Table 1, conventionally a high-molecular polysaccharide of glucomannan was used, whereas
It has been found that barley portal sugar, which has a molecular weight of ~10,000, is desirable in that it is easy to take and can be easily incorporated into other formulations. In addition, in Table 1, when determining obesity, one standard is shown based on the relationship between height and weight, for example, "Health Promotion Booklet No. 21" published by the Japan Dietetic Association. Graph inside (Figure 2)
Based on this, volunteers were selected to take the test. On the other hand, using 1 to 2 g of barley mondo sugar at a time will stop coughing. Antitussives, which are usually administered as medicines, have strong pharmacological activity, and when used for a long period of time, many of the side effects are addictive, cause gastrointestinal disorders, or have a bitter taste. It is characteristic that no side effects are observed. It is also effective as a physical condition improvement agent for people with weak constitutions, allergies, rashes caused by milk protein, egg white, mackerel, etc., or people with symptoms such as asthma. be. Table 2 shows the course of administration of barley mondo sugar for a few symptoms. Until now, there have been no reports that saccharides extracted from barley mondo have such actions (effects), but barley mondo sugar according to the present invention can be used as a therapeutic agent for these or as a constitution improving agent. It is also beneficial.
However, its mechanism of action is unknown.

【table】 [Brief explanation of the drawing]

Figure 1 shows GPC (gel permation)
We performed high-performance liquid chromatography (HPLC) based on the theory of chromatography, and show the results of measuring the molecular weight distribution of sugars extracted from Mugimondo. Figure 2 is a chart (graph) for determining obesity based on weight by height, which was used as a standard for determining obesity. When it is within the width of A, it indicates an almost normal weight. When it is within the width of the circle, it indicates a weight that requires attention. When it is above Ha, it indicates obesity and weight loss is necessary.

Claims (1)

[Claims] 1. Oligosaccharides or polysaccharides in which the total amount of saccharides extracted from barley monfu is 95% or more when quantified excluding water, and the molecular weight of the extracted saccharides is within the range of 500 to 10,000. A low-calorie food characterized by containing.