JPS6349069A - Cell fusion chamber - Google Patents

Abstract

PURPOSE:To reduce the necessary number of cell fusion chambers to be stocked and to improve the economy of cell fusion thereby, by using a cell fusion chamber capable of giving a different cell fusion chamber having different gap between electrodes by the exchange of an inner electrode. CONSTITUTION:A center pole 4 is fixed with an adhesive to the center of the bottom face of a dish-shaped main body of a chamber 2. A ring-shaped outer electrode 12 is placed on the bottom face of the vessel 2 concentrically to the center pole 4 and fixed with an adhesive. An inner electrode 14 is pressed and fixed to the bottom face of the vessel 2 with a pressing member 18. The inner electrode 14 has outer circumference opposite to the inner circumference of the outer electrode 12 and is positioned by inserting the inner circumference into a part of the outer circumference of the center pole 4. Since the cell fusion chamber can be changed to a different chamber having different gap between electrodes by exchanging the inner electrode, the cost of cell fusion chambers to be stocked can be remarkably lowered compared with the conventional process wherein a variety of cell fusion chambers having different electrode gaps should be stocked.

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention is used in devices for cell fusion and gene transfer, and is used to suspend cells between a pair of electrodes or to mix cells with genes, etc. Contains suspension.

The present invention relates to a cell fusion chamber that applies electrical stimulation to a cell suspension by applying a voltage between a pair of electrodes.

(Prior Art) In a conventional cell fusion chamber, a pair of metal electrodes are bonded and fixed in a container made of resin or glass with an adhesive.

(Problems to be Solved by the Invention) In cell fusion operations, it is necessary to change the voltage applied to the cell suspension or to make the distance between the pair of electrodes different due to limitations of the lightning source device. .

In conventional cell fusion chambers in which a pair of electrodes are bonded and fixed together, it is necessary to prepare many types of electrodes with different spacings between the electrodes. Since cell fusion chambers are expensive because the spacing between their electrodes must be precisely adjusted, providing a large number of cell fusion chambers is problematic in terms of cost.

The present invention is a patent characterized in that by making the interval between electrodes variable, the number of cell fusion chambers that must be kept is reduced, thereby reducing the trouble of storage and achieving economic efficiency by reducing the number of cell fusion chambers to be kept. It is.

(Means for Solving the Problems) In the cell fusion chamber of the present invention, a central metal fitting is fixed to the center of the bottom of a dish-shaped chamber body container with an adhesive, and a ring-shaped outer electrode is fixed to the center of the bottom surface of a dish-shaped chamber body container. It is arranged concentrically with the center metal fitting on the bottom surface and fixed with adhesive, the outer peripheral part faces the inner peripheral part of the outer electrode, and the inner peripheral part is fitted into a part of the outer peripheral part of the center metal fitting to determine the position. The inner electrode is pressed and fixed onto the bottom surface of the chamber main body using a presser metal fitting.

(Embodiment) FIG. 1 is a sectional view showing one embodiment of the present invention, and FIG. 2 is a partially cutaway plan view of the lid of the same embodiment. Note that FIG. 1 shows a state cut along line A-B in FIG. 2.

Reference numeral 2 denotes a chamber body container made of transparent glass, which is formed into a dish shape with an opening at the top. A center fitting 4 is bonded to the center of the bottom surface of the container 2 with an adhesive. A screw 6 is provided at the tip of the center of the center fitting 4, and the screw 6 is formed at a height that protrudes outward from M22. A portion 8 of the outer peripheral surface of the center fitting 4 has an outer diameter precisely machined. Also on the center metal fitting 4. A liquid reservoir 1o is formed.

2 is a ring-shaped outer electrode, which is positioned on the bottom surface of the container 2 concentrically with the center fitting 4 and bonded with an adhesive.

Reference numeral 14 denotes an inner electrode, and the inner circumferential surface 16 of the inner electrode 14 is precisely machined so as to be fitted into the outer circumferential surface 8 of the central metal fitting 4. The inner electrode 14 fitted into the center metal fitting 4 is held down by a presser metal fitting I8 screwed to the center of the center metal fitting 4, and the outer peripheral bottom surface of the inner electrode 14 is attached to the bottom surface of the container 2 in close contact.

An inner terminal 26 is fastened to the screw 6 at the tip of the center metal fitting 4 via a gasket 2o, a cover 22 made of transparent glass, and a gasket 24 at the upper part of the center of the center metal fitting 4. This maintains airtightness in the center of 1M22.

When the outer electrode 12 and the inner electrode 14 are shown in detail in FIG. The inner electrode 14 is pressed from above via a rubber sheet 15 and is in close contact with the bottom surface of the container 2.

Referring back to FIG. 1 again, a hole is formed in the periphery of the lid 22, and the outer terminal 32 is attached to the hole through gaskets 28 and 30 in an airtight manner. A spring current collector 34 is attached to the inside of the container of the outer terminal 32, and the tip of the current collector 34 contacts the outer electrode 12 to maintain electrical connection.

To perform the cell fusion operation in this example.

The cell suspension 22 is opened and the cell suspension is poured into the groove between the outer electrode 12 and the inner electrode 14. The inner electrode 14 is strongly held down by a holding fitting 18. Distilled water is poured into the liquid reservoir 10 of the central fitting 4 in order to prevent the cell suspension from evaporating.

An outer terminal 32 and a current collector 34 are attached to the lid 22 through gaskets 28 and 30, and the lid 22 is passed through the tip of the center metal fitting 4 and tightened with an inner terminal 26 through gaskets 20 and 24. As a result, the chamber main body container 2, lid 22, and gaskets 20, 24, 28, 30 maintain airtightness within the chamber main body and isolate it from the outside air, preventing invasion of various germs and also preventing evaporation of the cell S suspension. Ru.

Lead wires from a predetermined power source are connected to the inner terminal 26 and the outer terminal 32.

In this example, if a cell fusion chamber with different spacing between electrodes is required, remove the holding fitting 18.
The inner electrode 14 may be replaced with one having a different outer diameter.

The present invention is not limited to the illustrated cell fusion chamber equipped with ring-shaped electrodes, but can also be applied to cell fusion chambers equipped with parallel plate electrodes.

(Effects of the Invention) According to the present invention, cell fusion chambers with different inter-electrode spacings can be created by replacing the inner electrodes. Compared to preparing a cell fusion chamber, the cost of the cell fusion chamber that must be kept is significantly reduced.

[Brief explanation of drawings]

FIG. 1 is a sectional view showing an embodiment of the present invention, FIG. 2 is a partially cutaway plan view of the lid of the same embodiment, and FIG. 1 is a position taken along line A-B in FIG. This shows the state where it is cut. FIG. 3 is a partial sectional view showing details of the outer electrode and the inner electrode. 2... Chamber body container, 4... Center metal fitting, 12... Outer electrode. 14... Inner electrode, 18... Presser fitting.

Claims (1)

[Claims] (1) A center metal fitting is fixed to the center of the bottom of the dish-shaped chamber main body container with adhesive, and a ring-shaped outer electrode is arranged concentrically with the center metal fitting on the bottom surface of the chamber main body container. The inner electrode, whose outer periphery faces the inner periphery of the outer electrode and whose inner periphery is fitted into a part of the outer periphery of the central metal fitting to be positioned, is held down by the metal fitting on the bottom surface of the chamber body container. Cell fusion chamber that presses against and sticks to the cell fusion chamber.