JPS6342231B2 - - Google Patents
Info
- Publication number
- JPS6342231B2 JPS6342231B2 JP53153769A JP15376978A JPS6342231B2 JP S6342231 B2 JPS6342231 B2 JP S6342231B2 JP 53153769 A JP53153769 A JP 53153769A JP 15376978 A JP15376978 A JP 15376978A JP S6342231 B2 JPS6342231 B2 JP S6342231B2
- Authority
- JP
- Japan
- Prior art keywords
- vitamin
- mixture
- fraction
- separated
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 claims description 44
- 210000002966 serum Anatomy 0.000 claims description 39
- 238000004458 analytical method Methods 0.000 claims description 30
- 102000014914 Carrier Proteins Human genes 0.000 claims description 22
- 108091008324 binding proteins Proteins 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 20
- FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 claims description 17
- 239000002245 particle Substances 0.000 claims description 15
- 239000003610 charcoal Substances 0.000 claims description 13
- 239000000872 buffer Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 10
- 241000287828 Gallus gallus Species 0.000 claims description 9
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims description 9
- 239000011715 vitamin B12 Substances 0.000 claims description 9
- 230000027455 binding Effects 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 6
- 230000009137 competitive binding Effects 0.000 claims description 5
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 claims description 4
- HFWIMJHBCIGYFH-UHFFFAOYSA-N cyanoform Chemical compound N#CC(C#N)C#N HFWIMJHBCIGYFH-UHFFFAOYSA-N 0.000 claims description 4
- 239000013618 particulate matter Substances 0.000 claims description 4
- 230000002285 radioactive effect Effects 0.000 claims description 4
- 108010088751 Albumins Proteins 0.000 claims description 3
- 102000009027 Albumins Human genes 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 239000011720 vitamin B Substances 0.000 claims description 3
- 235000019156 vitamin B Nutrition 0.000 claims description 3
- 229940045999 vitamin b 12 Drugs 0.000 claims description 3
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 3
- 229930003270 Vitamin B Natural products 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 230000005389 magnetism Effects 0.000 claims 1
- 238000009835 boiling Methods 0.000 description 7
- 238000010790 dilution Methods 0.000 description 7
- 239000012895 dilution Substances 0.000 description 7
- NNFCIKHAZHQZJG-UHFFFAOYSA-N potassium cyanide Chemical compound [K+].N#[C-] NNFCIKHAZHQZJG-UHFFFAOYSA-N 0.000 description 6
- 239000012086 standard solution Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 229910020674 Co—B Inorganic materials 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 229920002678 cellulose Polymers 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000007790 solid phase Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000013048 microbiological method Methods 0.000 description 3
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- 241000195620 Euglena Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000002196 fr. b Anatomy 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- -1 sodium cyanide Chemical compound 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000195619 Euglena gracilis Species 0.000 description 1
- 101500028441 Gallus gallus Growth hormone-binding protein Proteins 0.000 description 1
- 101800000194 Growth hormone-binding protein Proteins 0.000 description 1
- 102400001066 Growth hormone-binding protein Human genes 0.000 description 1
- 102000011409 Transcobalamins Human genes 0.000 description 1
- 108010023603 Transcobalamins Proteins 0.000 description 1
- 235000012538 ammonium bicarbonate Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- KXZJHVJKXJLBKO-UHFFFAOYSA-N chembl1408157 Chemical compound N=1C2=CC=CC=C2C(C(=O)O)=CC=1C1=CC=C(O)C=C1 KXZJHVJKXJLBKO-UHFFFAOYSA-N 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 208000036654 deficiency anemia Diseases 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229940029329 intrinsic factor Drugs 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012543 microbiological analysis Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB5206977 | 1977-12-14 | ||
| GB7830301 | 1978-07-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS54116293A JPS54116293A (en) | 1979-09-10 |
| JPS6342231B2 true JPS6342231B2 (https=) | 1988-08-22 |
Family
ID=26266985
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15376978A Granted JPS54116293A (en) | 1977-12-14 | 1978-12-14 | Method of analyzing vitamin b12 |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS54116293A (https=) |
| AU (1) | AU519485B2 (https=) |
| CA (1) | CA1104911A (https=) |
| CH (1) | CH638053A5 (https=) |
| DE (1) | DE2853500A1 (https=) |
| FR (1) | FR2412074A1 (https=) |
| NL (1) | NL7812106A (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989012826A1 (en) * | 1988-06-15 | 1989-12-28 | Beckman Instruments, Inc. | Vitamin b12 assay |
-
1978
- 1978-12-11 AU AU42396/78A patent/AU519485B2/en not_active Expired
- 1978-12-12 DE DE19782853500 patent/DE2853500A1/de active Granted
- 1978-12-12 FR FR7834937A patent/FR2412074A1/fr active Granted
- 1978-12-13 NL NL7812106A patent/NL7812106A/xx not_active Application Discontinuation
- 1978-12-13 CA CA317,888A patent/CA1104911A/en not_active Expired
- 1978-12-14 CH CH1274878A patent/CH638053A5/de not_active IP Right Cessation
- 1978-12-14 JP JP15376978A patent/JPS54116293A/ja active Granted
Non-Patent Citations (1)
| Title |
|---|
| BRITISH JOURNAL OF HAEMATOLOGY=1973 * |
Also Published As
| Publication number | Publication date |
|---|---|
| NL7812106A (nl) | 1979-06-18 |
| CH638053A5 (en) | 1983-08-31 |
| FR2412074A1 (fr) | 1979-07-13 |
| AU4239678A (en) | 1979-06-21 |
| FR2412074B1 (https=) | 1984-05-25 |
| JPS54116293A (en) | 1979-09-10 |
| AU519485B2 (en) | 1981-12-03 |
| CA1104911A (en) | 1981-07-14 |
| DE2853500A1 (de) | 1979-06-21 |
| DE2853500C2 (https=) | 1988-04-14 |
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