JPS63276460A - Blood meal having high iron content and production thereof - Google Patents
Blood meal having high iron content and production thereofInfo
- Publication number
- JPS63276460A JPS63276460A JP62113217A JP11321787A JPS63276460A JP S63276460 A JPS63276460 A JP S63276460A JP 62113217 A JP62113217 A JP 62113217A JP 11321787 A JP11321787 A JP 11321787A JP S63276460 A JPS63276460 A JP S63276460A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- iron
- hemoglobin
- chitosan
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 104
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 53
- 239000005996 Blood meal Substances 0.000 title claims abstract description 10
- 238000004519 manufacturing process Methods 0.000 title claims description 7
- 239000008280 blood Substances 0.000 claims abstract description 52
- 210000004369 blood Anatomy 0.000 claims abstract description 52
- 229920001661 Chitosan Polymers 0.000 claims abstract description 10
- 244000144977 poultry Species 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 10
- 244000144972 livestock Species 0.000 claims description 5
- 229920006317 cationic polymer Polymers 0.000 claims description 3
- 206010013911 Dysgeusia Diseases 0.000 claims 1
- 108010054147 Hemoglobins Proteins 0.000 abstract description 23
- 102000001554 Hemoglobins Human genes 0.000 abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 10
- 241000283690 Bos taurus Species 0.000 abstract description 8
- 239000010802 sludge Substances 0.000 abstract description 6
- 238000003756 stirring Methods 0.000 abstract description 6
- 108091005804 Peptidases Proteins 0.000 abstract description 4
- 102000035195 Peptidases Human genes 0.000 abstract description 3
- 230000003301 hydrolyzing effect Effects 0.000 abstract description 2
- 239000003513 alkali Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000002198 insoluble material Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
- 238000007796 conventional method Methods 0.000 description 10
- 235000013305 food Nutrition 0.000 description 8
- 239000003814 drug Substances 0.000 description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 208000007502 anemia Diseases 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 230000000704 physical effect Effects 0.000 description 4
- 235000011121 sodium hydroxide Nutrition 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 208000007536 Thrombosis Diseases 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 150000003278 haem Chemical class 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 235000013594 poultry meat Nutrition 0.000 description 3
- 239000013076 target substance Substances 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 2
- 208000015710 Iron-Deficiency Anemia Diseases 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- -1 inorganic iron) Chemical compound 0.000 description 2
- 235000000396 iron Nutrition 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- IMWCPTKSESEZCL-SPSNFJOYSA-H (e)-but-2-enedioate;iron(3+) Chemical compound [Fe+3].[Fe+3].[O-]C(=O)\C=C\C([O-])=O.[O-]C(=O)\C=C\C([O-])=O.[O-]C(=O)\C=C\C([O-])=O IMWCPTKSESEZCL-SPSNFJOYSA-H 0.000 description 1
- UJKPHYRXOLRVJJ-MLSVHJFASA-N CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C Chemical compound CC(O)C1=C(C)/C2=C/C3=N/C(=C\C4=C(CCC(O)=O)C(C)=C(N4)/C=C4\N=C(\C=C\1/N\2)C(C)=C4C(C)O)/C(CCC(O)=O)=C3C UJKPHYRXOLRVJJ-MLSVHJFASA-N 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 206010067482 No adverse event Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 102000005158 Subtilisins Human genes 0.000 description 1
- 108010056079 Subtilisins Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012503 blood component Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 229960003569 hematoporphyrin Drugs 0.000 description 1
- 108010036302 hemoglobin AS Proteins 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 239000012770 industrial material Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 150000004033 porphyrin derivatives Chemical class 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、家畜類、家禽類の血液をもとにして得られる
鉄高含有血粉に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a blood meal with high iron content obtained from the blood of livestock and poultry.
「産業上の利用分計。“Industrial usage total.
家畜、家禽の屠殺に際して出る血液に関しては、種々の
加工がほどこされ、食品、医薬品の原料としてまた、工
業用素材として有効利用されている。The blood produced during the slaughter of livestock and poultry is subjected to various processes and is effectively used as a raw material for foods and medicines, and as an industrial material.
ことに、血液中の血餅を構成するヘモグロビンは加工食
品であるハムの着色剤や結着剤として多く利用され、今
後もその方面の利用は拡大するといわれている。一方、
最近のヘモグロビンの新しい応用分野に、鉄の供給源゛
として貧血者向けの栄養補助食品、あるいは医薬品への
利用が着目されるようになってきている。In particular, hemoglobin, which makes up blood clots, is often used as a coloring agent and binding agent for processed ham, and it is said that its use in this field will continue to expand in the future. on the other hand,
Recently, attention has been focused on new fields of application of hemoglobin, such as its use as a source of iron in nutritional supplements for anemic patients and in pharmaceuticals.
従来の鉄欠乏性貧血患者、又は貧血体質の人にはその治
療や予防を目的として、硫酸第１鉄、酸化鉄の様な無機
鉄、クエン酸鉄、フマール酸鉄の様な有機鉄（以下無機
鉄を含めて非ヘム鉄という）などの鉄剤を含む医薬品や
食品が用いられるのが一般的であったが、これら非ヘム
鉄は、概して体内における吸収効率が低く、それなりの
効果を求めようとする場合、摂取量を多くする必要があ
り、消化器官、特に胃を荒らす場合が多いことから敬遠
されがちであった。For patients with conventional iron deficiency anemia or people with anemia predisposition, inorganic iron such as ferrous sulfate and iron oxide, and organic iron (hereinafter referred to as iron) such as iron citrate and iron fumarate are recommended for the treatment and prevention of iron deficiency anemia. It was common to use pharmaceuticals and foods containing iron supplements such as non-heme iron (including inorganic iron), but these non-heme irons generally have low absorption efficiency in the body, so it is difficult to obtain a certain effect. In this case, it is necessary to consume a large amount, and it tends to be avoided because it often irritates the digestive system, especially the stomach.
他方、ヘモグロビン中に含まれる鉄（以下へ五鉄という
）は、前述の非ヘム鉄に比して、生物学的利用率も高く
、胃を始めとする消化気管への弊害もほとんど無く、新
しい鉄供給源として見直されてきている。On the other hand, iron contained in hemoglobin (hereinafter referred to as five irons) has a higher bioavailability than the non-heme iron mentioned above, has almost no adverse effects on the gastrointestinal tract including the stomach, and is a new It is being reconsidered as a source of iron supply.
ウシ、ブタ、馬、ニワトリなどの家畜、家禽類の血液中
成分の１０〜１５％を占めるヘモグロビンは、分子量６
０．０００〜７０．０００程の蛋白質で、その１分子に
４分子のプロトヘムが結合している。ヘモグロビン１分
子中に含まれる鉄は、０１８〜０．２４％程で、食物と
して人が摂取した場合、ヘモグロビンは消化吸収諮れ、
含まれる鉄は、人のヘモグロビンの合成に利用される。Hemoglobin, which accounts for 10-15% of the blood components of livestock such as cows, pigs, horses, and chickens, has a molecular weight of 6.
It is a protein of about 0.000 to 70,000, and each molecule has four molecules of protoheme bound to it. Iron contained in one molecule of hemoglobin is about 0.18 to 0.24%, and when ingested by humans as food, hemoglobin is difficult to digest and absorb.
The iron it contains is used in the synthesis of human hemoglobin.
我々人間は、ヘモグロビンをそのまま食べる習慣は無く
、普通は動物のレバー料理、又は肉料理などで、知らず
知らずのうちに摂っている訳であるが、前述した様に、
貧血の予防、治療を目的とした場合は、ヘモグロビンを
直接摂った方が、食物量、効率の面からしても、好まし
いことは言うまでもない。We humans don't have the habit of eating hemoglobin as it is, and we usually get it from animal liver dishes or meat dishes without realizing it, but as mentioned above,
When the purpose is to prevent or treat anemia, it goes without saying that it is preferable to directly ingest hemoglobin in terms of food quantity and efficiency.
１従来の技術。1 Conventional technology.
動物の血液からヘモグロビンを得るには、血液を摂取し
たのち溶血させ、遠心分離機によって血餅部分を分取し
、水、有機溶媒等による洗浄によっ−〔、繊維質、塩な
どの不純物を取り除いて精製される。To obtain hemoglobin from animal blood, the blood is ingested, hemolysed, the clot is separated using a centrifuge, and the clot is washed with water, organic solvents, etc. to remove impurities such as fibers and salts. removed and purified.
蛋白を主体とするヘモグロビンは、安定化のため、濃縮
乾燥操作によって得られる粉体（血粉−ヘモグロビン）
の型で、市場に提供されるのが一般的である。この血粉
を可食グレードに仕上げ、一部貧血者向けの、食品に利
用するわけであるが、血粉中の鉄含量は前にも述べた如
く、高々０．２４％程のものであり、例えば、成人女性
の１日の鉄必要量１０■を、血粉のみから摂取しようと
仮定すると、利用効率を考慮に入れると、１０ｇ以上を
食べる必要があり、血液特有の生臭さも手伝って、とて
も摂取不可能な量となってしまう、こうしたことから、
ヘモグロビンをある種の蛋白分解酵素を用いて処理し、
蛋白含量の少ない、つまり鉄含有率の高い血粉を製造し
、前述の用途に供しようとする動きが見られる。Hemoglobin, which is mainly composed of proteins, is made into a powder (blood meal - hemoglobin) obtained by concentration and drying in order to stabilize it.
It is generally offered on the market in this type. This blood powder is made into an edible grade and used as food for some anemic people, but as mentioned earlier, the iron content in blood powder is at most 0.24%, for example. If we assume that an adult woman's daily iron requirement is to be ingested only from blood meal, then if utilization efficiency is taken into account, it would be necessary to eat more than 10 g, and with the fishy smell characteristic of blood, it would be extremely difficult to ingest. Due to these reasons, the amount of
Hemoglobin is processed using certain proteolytic enzymes,
There is a movement to produce blood meal with a low protein content, that is, a high iron content, and to use it for the above-mentioned purposes.
そうした鉄属含有血粉製造の従来法の一例を明記すると
以下の様である。An example of the conventional method for producing iron-containing blood powder is as follows.
不純物を取除いた新鮮な牛血液１０（ｌを遠心分離し、
血餅的４０ｋｇを得る。これに２．５倍量の水を加えて
溶血させたのち、苛性ソーダの適量を用いてｐＨを８．
５に調整する。攪拌下に蛋白分解酵素（アルカラーゼ０
．６ Ｌ ノボインダストリー社製）１．２ｋｇを加
ｔ−ｃ、温度５０”Ｃ”Ｃ’３時間加水分解を行なう０
反応終了後、系の温度を上昇させて酵素を失活させた後
、９Ｈを塩酸を用いて４．０以下に調整する。この際、
析出する析出物（鉄含有率の高いヘモグロビン分解物）
を遠心分離機を用いて、ペースト状に回収し、さらにこ
のペーストを、水を加えて洗浄することにより、塩等の
夾雑物を取除いた後、再度遠心分離機を用いてペースト
状に目的物を回収する０次いで、スプレードライヤーを
用いて乾燥し、鉄属含有血粉２ 、 Ｏｋｇを得る。こ
こで得られた血粉は鉄を１．１％含有している。Centrifuge 10 liters of fresh bovine blood from which impurities have been removed,
Obtained 40 kg of blood clot. After adding 2.5 times the volume of water to hemolyse, adjust the pH to 8.0 using an appropriate amount of caustic soda.
Adjust to 5. Add proteolytic enzyme (alcalase 0) while stirring.
．． 6 L (manufactured by Novo Industries) 1.2 kg was added to t-c, and the temperature was 50"C"C' for 3 hours.
After the reaction is completed, the temperature of the system is raised to inactivate the enzyme, and then 9H is adjusted to 4.0 or less using hydrochloric acid. On this occasion,
Precipitates (decomposed products of hemoglobin with high iron content)
is collected into a paste using a centrifuge, and this paste is washed with water to remove impurities such as salt, and then processed into a paste using a centrifuge again. The material is then dried using a spray dryer to obtain 2.0 kg of iron-containing blood powder. The blood powder obtained here contains 1.1% iron.
しかし、鉄属含有血粉の市場への供給能力、商品価値を
考え合わせた場合、こうした従来の方法では、次の様な
問題があることが否めない、その１つは、製造工程中、
系のｐＨを酸性側へ移行させることによって析出してく
る目的物（鉄属含有ヘモグロビン）は、非常に微細な結
晶であることや、共存する分解液がアミノ酸等を多量に
含むため、気泡性に富むことなどから、遠心分離機やフ
ィルタープレス、その他の回収機による回収効率が非常
に悪く、回収コストが高くつくことになり、必然的に製
品単価にはね返り市場性も低くなる。実際、前述の製造
法によれば、鉄含量の理論値からしても３ 、５ ｋｇ
程の血粉が得られても良い筈であるが、２．０ｋｇ（５
７％の収率）と、回収率が悪い、他方、得られた鉄属含
有血粉は、血液由来物特有の生臭きを持っており、未加
工の血粉に比して、使用量が少なくて済むと言っても、
やはり食品として口にし難い欠点は、解消されていない
。However, when considering the ability to supply iron-containing blood powder to the market and its commercial value, it is undeniable that these conventional methods have the following problems.One of them is that during the manufacturing process,
The target substance (iron-containing hemoglobin) that is precipitated by shifting the pH of the system to the acidic side is a very fine crystal, and the coexisting decomposition liquid contains a large amount of amino acids, etc., so it is foamy. Because of the large amount of carbon dioxide, the collection efficiency of centrifuges, filter presses, and other collection equipment is extremely low, leading to high collection costs, which inevitably increases the product unit price and lowers marketability. In fact, according to the above-mentioned manufacturing method, even from the theoretical value of iron content, 3.5 kg
It should be fine to obtain about 2.0 kg (5 kg) of blood powder.
On the other hand, the iron-containing blood powder obtained has a fishy odor characteristic of blood-derived substances, and the amount used is small compared to unprocessed blood powder. Even if you say it's over,
As expected, the drawbacks that make it difficult to eat as food have not been resolved.
′発明が解決しようとする問題点」
本発明者らは、既に動物の血液の有効利用を目的として
、一連のポルフィリン化合物に看目し、ヘモグロビンか
ら得られるヘマトポルフィリン等、ポルフィリン誘導体
を用いた毛髪剤への応用について、特許公報昭５９−１
４００３、昭５６−４５３２に示される様な開発を行な
っているが、本発明は、血液の有効利用の一端である、
鉄属含有血粉の食品、医薬品への応用を考えるとき、前
述した従来法に見られる種々の欠点を改良し、市場性を
向上させることを目的として、鋭意研究した。``Problems to be Solved by the Invention'' The present inventors have already focused on a series of porphyrin compounds for the purpose of effectively utilizing animal blood, and have developed hair using porphyrin derivatives such as hematoporphyrin obtained from hemoglobin. Regarding application to agents, Patent Publication 1987-1
4003, 1983-4532, the present invention is one part of the effective utilization of blood.
When considering the application of iron-containing blood powder to foods and medicines, we conducted extensive research with the aim of improving the various drawbacks seen in the conventional methods mentioned above and improving marketability.
本発明は、家畜類、家禽類の血液からヘモグロビンを分
離、これを加水分解することによって鉄として０３％以
上を含む、生臭い味を持たない、鉄属含有血粉と、及び
その製造法にある。製造法としては、工程中に天然カチ
オン高分子であるキトサンを使用することを特徴とする
。詳しくは以下に開示する。The present invention provides an iron-containing blood meal that does not have a fishy taste and contains 0.3% or more iron by separating hemoglobin from the blood of livestock and poultry and hydrolyzing the same, and a method for producing the same. The manufacturing method is characterized by the use of chitosan, a natural cationic polymer, during the process. Details are disclosed below.
（実施例１）
不純物を取除いた新鮮な牛血液１ＱＱＯｋｇを遠心分離
し、血餅的４００ｋｇを得る。これに２゜５倍量の水を
加えて溶血許せる。（別に出発原料として牛血液を同様
に溶血きせ、ヘモグロビンのみを遠心分１ｌｌ１機等で
回収し、噴霧乾燥法などで乾燥させ血粉となしたのち、
殺菌処理を行なって可食グレードとして揚重されている
、一般名ヘモグロビンパウダー１５０ｋｇを１．５００
１の水に分散させたものでも可能）
次いで苛性ソーダの適量を用いて、ｐＨを８゜５に調整
した後、攪拌下に蛋白分解酵素８〜１２ｋｇを加えて、
温度５０℃で４〜５時間加水分解を行なう、酵素反応終
了後、系の温度を上昇させて、酵素を失活させた後、系
の温度を４０℃以下に降温づせ、塩酸を用いてｐＨを４
０〜６０、望ましくは５゜５に調整する。(Example 1) 1QQOkg of fresh bovine blood from which impurities have been removed is centrifuged to obtain 400kg of blood clot. Add 2.5 times the amount of water to this to allow hemolysis. (Separately, as a starting material, bovine blood is hemolyzed in the same way, only the hemoglobin is collected using a 1 liter centrifuge, etc., and it is dried using a spray drying method etc. to make blood powder.
150 kg of generic name hemoglobin powder, which has been sterilized and lifted into edible grade, is sold at 1.500 kg.
(It is also possible to disperse it in water of 1) Then, after adjusting the pH to 8.5 using an appropriate amount of caustic soda, add 8 to 12 kg of protease while stirring.
Hydrolysis is carried out at a temperature of 50°C for 4 to 5 hours. After the enzymatic reaction is completed, the temperature of the system is raised to inactivate the enzyme, and then the temperature of the system is lowered to 40°C or less and hydrochloric acid is used. pH to 4
Adjust to 0 to 60, preferably 5°5.
この操作によって、次第に不溶物（鉄含量が高いヘモグ
ロビン分解物）が析出する０次いで、これとは別にキト
サン５００ｇを、水１００１に攪拌下に分散させたのち
、希塩酸を用いてキトサンを溶解させてｙＡ整した粘稠
な液を、緩やかに攪拌しながら加える０次いで苛性ソー
ダを用いて系のｐＨを７０付近に上げる。この際、不溶
物はキトサン溶液の添加によって相互に凝集し、大きな
粒子となって系に懸濁される。Through this operation, insoluble matter (decomposed hemoglobin product with high iron content) is gradually precipitated.Next, separately, 500 g of chitosan is dispersed in 1,000 ml of water with stirring, and then the chitosan is dissolved using dilute hydrochloric acid. Add the adjusted viscous liquid while stirring gently. Next, raise the pH of the system to around 70 using caustic soda. At this time, the insoluble matter is mutually aggregated by the addition of the chitosan solution, and suspended in the system as large particles.
次いでフィルタープレス機を用いて、不溶物をスラッジ
として回収する。スラッジは湿体重量として、凡そ２０
０ｋｇ程の物である。スラッジは、再度反応タンクに返
し、２．０００１程の水を加えて攪拌しながら水洗を行
なう。The insoluble matter is then recovered as sludge using a filter press. The wet weight of sludge is approximately 20
It weighs about 0 kg. The sludge is returned to the reaction tank again, and about 2.000ml of water is added and washed with water while stirring.
０５〜１時間程の水洗後、再びフィルタープレス機を用
いて、鉄含量が高いヘモグロビン分解物のスラッジを回
収する０次に減圧乾燥機を用いて、７０〜８０℃の温度
で乾燥した後、粉砕し、目的とする鉄属含有血粉を得る
。これによって得られる鉄属含有血粉の鉄含有量は、１
．０〜１．２％程であり、出発原料牛血液１．ＯＯＯｋ
ｇ（牛ヘモグロビンパウダー１５０ｋｇ）から、３５〜
４゜ｈＢ程の鉄属含有血粉が得られ、収率は９０〜９５
％の高い値となる。After washing with water for about 5 to 1 hour, the filter press machine is used again to recover the sludge of the decomposed hemoglobin product, which has a high iron content. After drying at a temperature of 70 to 80 °C using a vacuum dryer, Pulverize to obtain the desired iron-containing blood powder. The iron content of the iron-containing blood powder obtained by this method is 1
．． It is about 0 to 1.2%, and the starting material bovine blood 1. OOOk
g (150 kg of bovine hemoglobin powder), 35~
Iron-containing blood powder of about 4゜hB was obtained, and the yield was 90-95.
This results in a high value of %.
また得られた血粉は、従来法によって得られた血粉に比
して生臭い臭い、味が極端に少なく、食品としての価値
も、非常に高いものであった。Furthermore, the obtained blood powder had an extremely low fishy odor and taste compared to blood powder obtained by conventional methods, and its value as a food product was also very high.
（物性及び作用又は効果の確認）
前記実施例で示す鉄属含有血粉に係る物性等の特徴につ
いての試験の結果を、第１〜２表及び第１〜３図、第４
図に示す。(Confirmation of physical properties and actions or effects) The results of tests on the physical properties and other characteristics of the iron-containing blood powder shown in the above examples are shown in Tables 1 to 2, Figures 1 to 3, and 4.
As shown in the figure.
尚、第４図において用いた検液は、あらかじめ、試料０
．５ｇを１％ＮａＯＨ１愛中に溶解したものを用いて行
なった。又、第４図の成績結果（０，Ｄ値）から、末法
によって得られた鉄属含有血粉を求めてみると、その成
績結果は、第２表に示すごとくとなる。In addition, the test solution used in Fig. 4 was prepared in advance from sample 0.
．． The test was carried out using 5 g dissolved in 1% NaOH. In addition, when the iron-containing blood powder obtained by the powder method was determined from the results (0, D value) in FIG. 4, the results were as shown in Table 2.
「第１表、鉄面含有血粉の物性
１第２表」０．Ｄ値から求めた分解率
〔発明の効果〕
本発明（実施例）により得られた鉄面含有血粉は、鉄と
して０．３％以上を含み、血液由来物質特有の、生臭い
味が殆ど無いことが、大きな特徴である。また、酵素に
よる加水分解終了後、目的物を回収するために、ｐＨを
４．０以下に調整して析出させなければならなかった従
来法に比して、ｐ）（ａ、ｏ〜７．０の中性域で効率よ
く回収できるため、従来法で得た鉄面含有血粉の様に、
食した時の低ｐＨによる刺激味が、全くないことも大き
なメリットである。"Table 1, Physical Properties of Blood Powder Containing Iron Surface 1 Table 2" 0. Decomposition rate determined from the D value [Effects of the invention] The iron-containing blood powder obtained according to the present invention (example) contains 0.3% or more of iron and has almost no fishy taste characteristic of blood-derived substances. is a major feature. In addition, compared to the conventional method, in which the pH had to be adjusted to 4.0 or less to precipitate in order to recover the target product after hydrolysis by the enzyme was completed, p) (a, o to 7. Since it can be efficiently collected in the neutral range of 0, it can be collected efficiently in the neutral range of
Another major advantage is that there is no irritating taste due to the low pH when eaten.
また、天然カチオン性高分子キトサンを用いたことは、
次の点でも製造効果の向上に役立っている。つまり従来
法によっては、加水分解後にｐＨを調整することによっ
て、析出する目的物（鉄面含有血粉となる）があまりに
も微細であるため、固〜液分離が非常に困難で、一般的
なバッチ、連続遠心分離機による回収や、フィルタープ
レス機などによる回収が商業ベースでは、殆んど不可能
であった。この点、キトサンにより凝集した目的物は、
大きな塊状を形成し、最も操作性の良いフィルタープレ
ス機で効率よく回収できることとなった。In addition, the use of natural cationic polymer chitosan
The following points also help improve manufacturing efficiency. In other words, depending on the conventional method, by adjusting the pH after hydrolysis, the target substance that precipitates (which becomes iron-containing blood powder) is so fine that solid-liquid separation is extremely difficult. However, on a commercial basis, recovery using continuous centrifuges, filter presses, etc. is almost impossible. In this respect, the target substance aggregated by chitosan is
It formed large clumps and could be efficiently collected using a filter press machine, which has the highest operability.
本発明による効果は、上記した如くであり、収率的にも
良好で操作性に優れていること、また従来、加工食品、
医薬品への血粉の利用に当って、臭い、味の欠点から用
途が限定されてきたわけであるが、本発明によって加工
食品、医薬品への応用が大きく拡大することと期待でき
る。The effects of the present invention are as described above, and the yield is good and the operability is excellent.
The use of blood powder in medicines has been limited due to the drawbacks of odor and taste, but it is expected that the present invention will greatly expand its application to processed foods and medicines.
第１〜３図は、にＢｒ錠剤法による、赤外線吸収スペク
トルである。
第１図は、末法による鉄面含有血粉、第２図は、従来法
による鉄面含有血粉、第３１３０は、未処理血粉（酸素
分解する前のもの）。
第４図は、本発明法による鉄面含有血粉、従来法による
鉄面含有血粉、未処理血粉（酸素分解する前のもの）の
紫外部、可視部吸収スペクトルを示す。
第４図中、Ａは末法による鉄面含有血粉、Ｂは、従来法
による鉄面含有血粉、Ｃは、加水分解する前の未処理血
粉。Figures 1 to 3 are infrared absorption spectra obtained by the Br tablet method. Fig. 1 shows iron-containing blood powder obtained by the powder method, Fig. 2 shows iron-containing blood powder obtained by the conventional method, and No. 3130 shows untreated blood powder (before decomposition with oxygen). FIG. 4 shows the ultraviolet and visible absorption spectra of iron-containing blood powder obtained by the method of the present invention, iron-containing blood powder obtained by the conventional method, and untreated blood powder (prior to oxygen decomposition). In FIG. 4, A is iron-containing blood powder obtained by the powder method, B is iron-containing blood powder obtained by the conventional method, and C is untreated blood powder before hydrolysis.
Claims (2)
トサン処理し、血液由来の特有の生臭い味が殆んど無い
ことを特徴とする、鉄高含有血粉。(1) A blood powder with high iron content, which contains 0.3% or more of iron when quantified, is treated with chitosan, and has almost no fishy taste peculiar to blood.
有血粉の製造工程において、天然カチオン高分子である
キトサンを使用することを特徴とする、鉄高含有血粉の
製造法。(2) A method for producing a high-iron blood meal, which is characterized by using chitosan, a natural cationic polymer, in the process of producing a high-iron blood meal obtained by processing the blood of livestock and poultry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62113217A JPS63276460A (en) | 1987-05-08 | 1987-05-08 | Blood meal having high iron content and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62113217A JPS63276460A (en) | 1987-05-08 | 1987-05-08 | Blood meal having high iron content and production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63276460A true JPS63276460A (en) | 1988-11-14 |
| JPH0242467B2 JPH0242467B2 (en) | 1990-09-21 |
Family
ID=14606541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62113217A Granted JPS63276460A (en) | 1987-05-08 | 1987-05-08 | Blood meal having high iron content and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63276460A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0413680A (en) * | 1990-05-02 | 1992-01-17 | Asahi Chem Ind Co Ltd | Production of hemoferrum complex |
-
1987
- 1987-05-08 JP JP62113217A patent/JPS63276460A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0413680A (en) * | 1990-05-02 | 1992-01-17 | Asahi Chem Ind Co Ltd | Production of hemoferrum complex |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0242467B2 (en) | 1990-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH0458946B2 (en) | ||
| JP2509436B2 (en) | Non-phosphorylated peptide | |
| SU1308183A3 (en) | Method of producing extracts,possessing antiulcerous,insulin-like,antilipidic activity promoting nutrition assimilation,from animal carcasses | |
| JPH05505304A (en) | Method for producing enzymatic hydrolyzate | |
| JP2023510941A (en) | Method for producing clam active peptide | |
| JP4955944B2 (en) | Chondroitin calcium sulfate and method for producing the same | |
| CN108077940A (en) | A kind of abalone peptide removes fishy smell technique | |
| NO163859B (en) | PROCEDURE FOR THE PREPARATION OF THERAPEUTIC ACTIVE SUCCINYLATED SUSTAINABLE PROTEINS. | |
| JPS63258559A (en) | Peptide mixture rich in dipeptide and tripeptide especially usable in artificial nutrition intake and dietary method, and its production and use | |
| EA006435B1 (en) | Method for producing a nutrition additive, an additive and its use | |
| CN109293766A (en) | The method of collagen polypeptide is extracted from fish scale | |
| JPH03168064A (en) | Making of powdered flavoring foodstuff using blood of domestic animal | |
| KR100563361B1 (en) | Ferro-succinyl casein complex, preparation method thereof and pharmaceutical composition containing same | |
| JPS63276460A (en) | Blood meal having high iron content and production thereof | |
| JP2001275599A (en) | Method for producing soybean-processed product having low purine content | |
| JPH0354958B2 (en) | ||
| CN108611390A (en) | A method of preparing low bitter taste buffalo's milk casein antioxidant peptide powder | |
| EP2064958B1 (en) | Method for collecting blood and transforming same into a hydrolyzed protein using slaughter animal blood, such as to obtain hydrolyzed blood protein | |
| JPH01273560A (en) | Hem iron solution or beverage containing the same solution | |
| US6217932B1 (en) | Method of obtaining haemin from slaughter blood | |
| KR20060005770A (en) | Preparation of water soluble heme-iron polypeptide complex from animal whole blood or clotted blood | |
| Seto et al. | Toxicity in Oil Meal, Properties of the the Toxic Factor in Trichloroethylene-Extracted Soybean Oil Meal | |
| RU2366263C2 (en) | Broth with preventive properties, containing protein hydrolisate and protein hydrolisate production method | |
| JPS6163247A (en) | Production of globin protein | |
| JPH03160951A (en) | Hemoferrum-containing biscuit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313532 |
|
| R371 | Transfer withdrawn |
Free format text: JAPANESE INTERMEDIATE CODE: R371 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313532 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (event date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20070921 Year of fee payment: 17 |