JPS63160580A - Culture of callus of crocus genus plant - Google Patents
Culture of callus of crocus genus plantInfo
- Publication number
- JPS63160580A JPS63160580A JP61306620A JP30662086A JPS63160580A JP S63160580 A JPS63160580 A JP S63160580A JP 61306620 A JP61306620 A JP 61306620A JP 30662086 A JP30662086 A JP 30662086A JP S63160580 A JPS63160580 A JP S63160580A
- Authority
- JP
- Japan
- Prior art keywords
- callus
- medium
- crocus
- plant
- genus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010020649 Hyperkeratosis Diseases 0.000 title claims abstract description 53
- 241000196324 Embryophyta Species 0.000 title claims abstract description 25
- 241000596148 Crocus Species 0.000 title claims abstract description 19
- 235000004237 Crocus Nutrition 0.000 title claims abstract description 18
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 239000006870 ms-medium Substances 0.000 claims abstract description 7
- 239000003375 plant hormone Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 239000002609 medium Substances 0.000 abstract description 32
- 244000124209 Crocus sativus Species 0.000 abstract description 16
- 235000015655 Crocus sativus Nutrition 0.000 abstract description 16
- 239000004248 saffron Substances 0.000 abstract description 16
- 235000013974 saffron Nutrition 0.000 abstract description 16
- SGAWOGXMMPSZPB-UHFFFAOYSA-N safranal Chemical compound CC1=C(C=O)C(C)(C)CC=C1 SGAWOGXMMPSZPB-UHFFFAOYSA-N 0.000 abstract description 14
- 230000006698 induction Effects 0.000 abstract description 7
- 235000017509 safranal Nutrition 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 7
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 abstract description 6
- 229920001817 Agar Polymers 0.000 abstract description 4
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 abstract description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 abstract description 4
- 239000008272 agar Substances 0.000 abstract description 4
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 abstract description 4
- 229960001669 kinetin Drugs 0.000 abstract description 4
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract description 3
- 239000004062 cytokinin Substances 0.000 abstract description 3
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 abstract description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 abstract description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 abstract description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 abstract description 2
- 229960001285 quercetin Drugs 0.000 abstract description 2
- 235000005875 quercetin Nutrition 0.000 abstract description 2
- 235000013599 spices Nutrition 0.000 abstract description 2
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 abstract description 2
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 abstract description 2
- 229940023877 zeatin Drugs 0.000 abstract description 2
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 abstract 2
- 229930195732 phytohormone Natural products 0.000 abstract 2
- LGGQQYXFFSOIJY-UHFFFAOYSA-N 2-(3-methylbut-2-enyl)-7h-purin-6-amine Chemical compound CC(C)=CCC1=NC(N)=C2NC=NC2=N1 LGGQQYXFFSOIJY-UHFFFAOYSA-N 0.000 abstract 1
- 241000189665 Colchicum autumnale Species 0.000 abstract 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 abstract 1
- -1 ethylenegibberellin Natural products 0.000 abstract 1
- 239000005556 hormone Substances 0.000 abstract 1
- 229940088597 hormone Drugs 0.000 abstract 1
- 239000002266 menstruation inducing agent Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 6
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 5
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 3
- 229930024421 Adenine Natural products 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- PANKHBYNKQNAHN-JTBLXSOISA-N Crocetin Natural products OC(=O)C(\C)=C/C=C/C(/C)=C\C=C\C=C(\C)/C=C/C=C(/C)C(O)=O PANKHBYNKQNAHN-JTBLXSOISA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- PANKHBYNKQNAHN-JUMCEFIXSA-N carotenoid dicarboxylic acid Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C(=O)O)C=CC=C(/C)C(=O)O PANKHBYNKQNAHN-JUMCEFIXSA-N 0.000 description 2
- PANKHBYNKQNAHN-MQQNZMFNSA-N crocetin Chemical compound OC(=O)C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)C(O)=O PANKHBYNKQNAHN-MQQNZMFNSA-N 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 240000008620 Fagopyrum esculentum Species 0.000 description 1
- 101100289255 Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) lnt gene Proteins 0.000 description 1
- 101100208473 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) lcm-2 gene Proteins 0.000 description 1
- 101150087495 PPM2 gene Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、クロッカス属植物のカルスの培養方法に関す
る。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a method for culturing callus of a plant of the genus Crocus.
クロッカス属植物の細胞、例えばサフランのめしべには
、サフラナールやクロセチンが含有されておシ、これら
の物質は通経剤、香辛料などに利用されその需要は大き
い。Cells of plants of the genus Crocus, such as the pistils of saffron, contain safranal and crocetin, and these substances are used as emmenagogues, spices, etc., and are in high demand.
しかしながら、サフランのめしべは少量にしか採れず、
したがってこれを原料として得られるサフラナール等の
物質は非常に高価なものである。However, saffron pistils can only be harvested in small quantities;
Therefore, substances such as safranal obtained using this as a raw material are very expensive.
ところで、クロッカス属植物のカルスには、サフランの
めしべと同様にサフラナールやクロセチンが含有されて
おり、そのカルスからサフラナール等の物質を採取する
方法もある。しかし、クロッカス植物のカルスば、従来
の寒天培地で培養すると、培養中に枯死しやすく、した
がって、従来法においては、そのカルスの大量培養がで
きないので、サフラナール等の物質は安価に得られな込
という問題があった。By the way, the callus of plants of the genus Crocus contains safranal and crocetin, similar to the pistil of saffron, and there is a method for collecting substances such as safranal from the callus. However, when the callus of crocus plants is cultured on a conventional agar medium, it tends to wither and die during cultivation. Therefore, it is not possible to cultivate large quantities of callus using conventional methods, and substances such as safranal cannot be obtained at low cost. There was a problem.
本発明は、クロッカス属植物のカルスを大量に培養する
のに適した方法を提供することを目的とするものである
。An object of the present invention is to provide a method suitable for culturing a large amount of callus of plants belonging to the genus Crocus.
本発明者等は、従来の寒天培地中にはクロッカス属植物
のカルスが増殖するのを阻害する物質が含まれているの
ではないかとの仮説をもとに、種種の培地について培養
試験を繰返した結果、クロッカス属植物のカルスの増殖
だ適した培地を検出するに至り、本発明を完成したもの
である。Based on the hypothesis that conventional agar media may contain substances that inhibit the growth of callus of plants of the genus Crocus, the inventors repeatedly conducted culture tests on various types of media. As a result, a medium suitable for the growth of callus of plants of the genus Crocus was detected, and the present invention was completed.
本発明はクロッカス属植物のカルスの培養方法に関し、
クロッカス属植物のカルスを植物ホルモン添加のＭＳ培
地で培養することを特徴とするものである。The present invention relates to a method for cultivating callus of plants of the genus Crocus,
This method is characterized by culturing callus of plants of the genus Crocus in an MS medium supplemented with plant hormones.
本発明でクロノカス属植物とは、サフラン、イヌサフラ
ン及びクロッカス等のクロッカス属に属する植物をいう
。In the present invention, plants of the genus Cronocus refer to plants belonging to the genus Crocus, such as saffron, dog saffron, and crocus.
また、カルスとは植物組織より生ずる脱分化細胞のこと
をいう。In addition, callus refers to dedifferentiated cells generated from plant tissue.
本発明の実施に当って、まず、植物ホルモンを添加した
ＭＳ培地を用意する。ここで植物ホルモンとは、オーキ
シン、２．４−Ｄ（２，４ジクロロフエノキシ酢酸）
、 ＮＡＡ （ナフタレン酢酸）、サイトカイニン、ア
プシジン酸、エチレンゾペレリン、カイネチン（６・フ
ルフリルアミノプリン）、ゼアチン（６・ヒドロキフイ
ン被ンテニルアミノプリン）、ベンジルアデニン及び２
・インペンテニルアデニン等の、培地に微量添加するこ
とにより植物組織の生長や分化を促す物質をいう。ＭＳ
培地に対する最適の添加量は植物ホルモンの種類によっ
て異なり、例えば、２．４−Ｄの場合０．３〜３ ｐｐ
ｍ 、 ＮＡＡの場合０．３〜１０ｐｐｍである。また
、これらの植物ホルモンのうち、オーキシン、サイトカ
イニン、カイネチン、ゼカチン、ペンノルアデニン及び
２・インインテニルアデニンの二種以上の組み合わせを
用いると、カルス培養に特に効果的である。尚、ＭＳ培
地とは、ＭｕｒａｓｈｉｇｅとＳｋｏｏｇの基本培地の
ことをいう。In carrying out the present invention, first, an MS medium to which a plant hormone is added is prepared. Here, plant hormones include auxin, 2.4-D (2,4 dichlorophenoxyacetic acid)
, NAA (naphthalene acetic acid), cytokinin, apsidic acid, ethylenezopererine, kinetin (6-furfurylaminopurine), zeatin (6-hydrokifuin-tenylaminopurine), benzyladenine and 2
- Substances such as impentenyl adenine that promote the growth and differentiation of plant tissues by adding small amounts to the culture medium. M.S.
The optimal amount added to the medium varies depending on the type of plant hormone; for example, in the case of 2.4-D, it is 0.3 to 3 pp.
m, in the case of NAA, it is 0.3 to 10 ppm. Furthermore, among these plant hormones, the use of a combination of two or more of auxin, cytokinin, kinetin, zecatin, pennoladenine, and 2-intenyladenine is particularly effective for callus culture. Note that the MS medium refers to Murashige and Skoog's basic medium.
次に、上記培地にクロノカス属植物のカルスを植え付け
て培養する。尚、クロッカス属植物のカルスを得るには
、クロッカス属植物の組織（球根、茎、めしべ、おしべ
、根等）の切片を滅菌した後、培地に植え付けてカルス
誘導を行なえばよい。ここで用いる培地は、（本発明に
おいてクロノカス属植物のカルスを培養するのに用いる
のと同じ）植物ホルモン添加のＭＳ培地のほか、従来の
寒天培地であっても差し支えない。尚、カルス誘導に植
物ホルモン添加培地を用いると、得られたカルスを培養
培地に植えかえしなくてもよいので便利であるＯ
カルスの培養は、小規模に行なうときは試験管やフラス
コを用いればよいが、大規模に行なうときはツヤ−ファ
ーメンタ−やタンクを用いるとよい。Next, callus of a plant of the genus Chronocus is planted in the above medium and cultured. In order to obtain a callus from a Crocus genus plant, callus induction may be performed by sterilizing a section of tissue (bulb, stem, pistil, stamen, root, etc.) of a Crocus genus plant, and then planting it in a medium. The medium used here may be a conventional agar medium as well as an MS medium supplemented with plant hormones (the same as used for culturing callus of plants belonging to the genus Cronochus in the present invention). In addition, it is convenient to use a plant hormone-supplemented medium for callus induction since there is no need to replant the obtained callus in the culture medium. However, when doing it on a large scale, it is better to use a gloss fermentor or tank.
このようにして植え付けた切片は約１ケ月後にカルス化
し、さらにとのカルスを約１ケ月培養すると初期カルス
の約１０倍容量に生成したカルスを得ることができる。The thus-planted sections turn into callus after about one month, and if the callus is further cultured for about one month, callus with a volume about 10 times that of the initial callus can be obtained.
実施例１
（イ）培地の調整
ＭＳ基本組成からなる培地に、２．４−Ｄを１．０ｐｐ
ｍ、−４＝”７チンを０．５ｐｐｍ添加した後ＰＨ５，
８Ｋ調整して液体培地を得た。Example 1 (a) Adjustment of medium 1.0 pp of 2.4-D was added to a medium consisting of the basic composition of MS.
m, -4=”PH5 after adding 0.5 ppm of 7tin,
A liquid medium was obtained by adjusting 8K.
（ロ）組織の調整
サフランの球根を滅菌（７０％エタノール水溶液に１分
間浸漬、２％次亜塩素酸水溶液に１５分間浸ｆＮ）後、
切断して厚さ２ｍＷ！、タテ・ヨコ５ ｍｍの切片を得
た。(b) Tissue preparation After sterilizing the saffron bulbs (immersed in 70% ethanol aqueous solution for 1 minute, immersed in 2% hypochlorous acid aqueous solution for 15 minutes),
Cut to 2mW thickness! , 5 mm vertical and horizontal sections were obtained.
（ハ）カルス誘導
第１図（ａ）に示すように、滅菌した試験管１内に、液
体培地３を入れ、この液体培地３に下部が漬かり、上部
が液面から出るようにペノパーウィンク（Ｐ紙）４を入
れ、このベソノクーウィンク４の頂部にサフラン切片Ｘ
を植え付け、アルミホイルで蓋をした後、２５℃の光照
射恒温器に入れた。植え付後３０日で切片がカルス化し
た。(c) Callus induction As shown in Figure 1 (a), put a liquid medium 3 into a sterilized test tube 1, and use a penoper wink so that the lower part is immersed in the liquid medium 3 and the upper part is above the liquid surface. (P paper) 4 and put a piece of saffron
After planting and covering with aluminum foil, the plants were placed in a light-irradiated incubator at 25°C. The sections formed calluses 30 days after planting.
に）培養
カルス化した組織を培地更新（（イ）で調整した培地使
用）してさらに１ケ月間培養を続けたところ、第１図（
ｂ）に示すように、増殖したカルスＹを得ることができ
た。When the cultured callus tissue was cultured (using the medium prepared in (a)) and cultured for another month, the results were as shown in Figure 1 (
As shown in b), proliferated callus Y could be obtained.
実施例２
培地として、ＭＳ基本組成からなる培地にナフタレン酢
酸３．０ ｐｐｍ 、！： ヘンシルアデニン２． Ｏ
ｐｐｍ 全添加した後ＰＨ５，８に調整した培地を用い
たほかは実施例１と同じ方法で、組織の調整、カルス誘
導及び培養を行なったところ、実施例１と同様に増殖し
たカルスを得ることができた。Example 2 As a medium, 3.0 ppm of naphthalene acetic acid was added to a medium consisting of the basic composition of MS. : Hensyl Adenine 2. O
Tissue preparation, callus induction, and culture were performed in the same manner as in Example 1, except that a medium adjusted to pH 5.8 after adding all ppm was used, and callus that proliferated in the same manner as in Example 1 was obtained. was completed.
実施例３
（イ）培地の調整
ＭＳ基本組成からなる培地に２．４−Ｄを３ ｐｐｍ２
・イン梗ンテニルアデニンを１ ｐｐｍ及びアガロース
を１％添加した後ＰＨ５，８に調整した固型培地を得た
。Example 3 (a) Adjustment of medium 3 ppm2 of 2.4-D was added to a medium consisting of MS basic composition.
- After adding 1 ppm of intravenous tenyladenine and 1% of agarose, a solid medium was obtained which was adjusted to pH 5.8.
（ロ）組織の調整
サフランの球根を実施例１と同じよって処理して滅菌し
たサフランの切片を得た。(b) Tissue preparation Saffron bulbs were treated in the same manner as in Example 1 to obtain sterilized saffron sections.
（ｆＪカルス誘導
第２図（ａ）に示すように、滅菌した三角フラスコ１′
内の固型培地上にサフランの切片Ｘを植え付け、三角フ
ラスコ１′の開口部をアルミホイル２で蓋をした後２５
°Ｃの光照射恒温器に入れた。植え術後３０日で切片が
カルス化した。(fJ callus induction As shown in Fig. 2 (a), a sterilized Erlenmeyer flask 1'
After planting the saffron slices
It was placed in a light irradiation incubator at °C. The sections formed calluses 30 days after implantation.
に）培養
カルス化した組織を培地更新（（イ）で調整した培地使
用）して１ケ月間培養を続けたところ、第２図（ｂ）に
示すように、増殖したカルスＹを得ることができた。(b) When cultured callus tissue was cultured for one month after renewing the culture medium (using the medium prepared in (a)), it was possible to obtain callus Y that proliferated as shown in Figure 2 (b). did it.
実施例４
（イ）培地の調整
ＭＳ基本組成からなる培地にＮＡＡ ５． Ｏｐｐｍと
ベンノルアデニン３． Ｏｐｐｍを添加した後ＰＨ５，
８に調整した液体培地
（ロ）組織の調整
サフランの球根を実施例１と同じように処理して滅菌し
たサフランの切片を得た。Example 4 (a) Adjustment of medium NAA was added to the medium consisting of MS basic composition 5. Oppm and Bennoadenine3. PH5 after adding Oppm,
Liquid medium adjusted to 8. (b) Tissue adjustment Saffron bulbs were treated in the same manner as in Example 1 to obtain sterilized saffron sections.
（ハ）カルス誘導・培養
上記培地とサフランの切片を用いて、実施例】と同じ方
法でカルス誘導とその培養を行ったところ、初期カルス
の約１０倍容量のカルス組織を得ることができた。(c) Callus induction and culture When callus was induced and cultured in the same manner as in Example using the above medium and saffron slices, callus tissue with a volume approximately 10 times that of the initial callus could be obtained. .
上述のように本発明によれば、クロノカス属植物のカル
スを枯死させることなく、大量に培養することができ、
したがって、クロノカス属植物の有効成分であるサフラ
ナールやケルセチンを安価に得ることが可能である。As described above, according to the present invention, callus of plants of the genus Chronocus can be cultured in large quantities without dying,
Therefore, it is possible to obtain safranal and quercetin, which are the active ingredients of plants of the genus Chronocus, at low cost.
第１図は試験管内の液体培地に挿入したにノ・や−ウィ
ック上でサフランの組織切片をカルス誘導しかつそのカ
ルスを培養する場合を示したもので第１図（ａ）は組織
切片を４ツノ９−ウィック上に植え付けた直後の状態を
示し、第１図（ｂ）は誘導したカサフランの組織切片を
カルス誘導し、かつそのカルスを培養する場合を示した
もので、第２図（ａ）はカルス組織を培地に植え付けた
直後の状態を示し、第２図（ｂ）は誘導したカルスを培
養はじめてから１ケ月後の状態を示す。
１：試験管、１′：三角フラスコ、３：培地、４：被ソ
バ−ウィック、Ｘ：サフラン組織切片、Ｙ：カルス。
特許出願人 キューピー株式会社
第１ｆｆｉ
（０） （ｂ）
第２図
（ａ） （ｂ）Figure 1 shows the case of inducing callus from a tissue section of saffron on a wick inserted into a liquid medium in a test tube and culturing the callus. Figure 1 (b) shows the state immediately after planting on a 4-horned 9-wick, and Figure 1 (b) shows the case where a callus is induced from a tissue section of induced Casafran and the callus is cultured, and Figure 2 ( Figure 2 (a) shows the state immediately after the callus tissue was planted in the medium, and Figure 2 (b) shows the state one month after the culture of the induced callus began. 1: Test tube, 1': Erlenmeyer flask, 3: Medium, 4: Soba wick, X: Saffron tissue section, Y: Callus. Patent applicant Kewpie Co., Ltd. No. 1ffi (0) (b) Figure 2 (a) (b)
Claims (1)
地で培養することを特徴とするクロッカス属植物のカル
スの培養方法。A method for culturing a callus of a plant of the genus Crocus, which comprises culturing the callus of a plant of the genus Crocus in an MS medium supplemented with a plant hormone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61306620A JPS63160580A (en) | 1986-12-24 | 1986-12-24 | Culture of callus of crocus genus plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61306620A JPS63160580A (en) | 1986-12-24 | 1986-12-24 | Culture of callus of crocus genus plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS63160580A true JPS63160580A (en) | 1988-07-04 |
Family
ID=17959275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61306620A Pending JPS63160580A (en) | 1986-12-24 | 1986-12-24 | Culture of callus of crocus genus plant |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63160580A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63237783A (en) * | 1987-03-26 | 1988-10-04 | Koopu Chem Kk | Tissue culture of saffron stigma |
| JP2008074752A (en) * | 2006-09-20 | 2008-04-03 | Kagawa Univ | Method for using rare saccharide for promotion or control of growth of vegetable shoot |
-
1986
- 1986-12-24 JP JP61306620A patent/JPS63160580A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63237783A (en) * | 1987-03-26 | 1988-10-04 | Koopu Chem Kk | Tissue culture of saffron stigma |
| JP2008074752A (en) * | 2006-09-20 | 2008-04-03 | Kagawa Univ | Method for using rare saccharide for promotion or control of growth of vegetable shoot |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vyskot et al. | Clonal propagation of cacti through axillary buds in vitro | |
| YAMADA et al. | Callus induction in rice, Oryza sativa L. | |
| CN107094625A (en) | A kind of southerm yew tissue culture of sprout mating system | |
| RU2757318C1 (en) | Method for obtaining microplants of medicinal plant stephania glabra (roxb.) miers | |
| Ramakrishna et al. | High efficient somatic embryogenesis development from leaf cultures of Citrullus colocynthis (L.) Schrad for generating true type clones | |
| JPS6137029A (en) | Regeneration of sunflower by germ and organ formation | |
| IIZUKA et al. | Tubular floret culture of chrysanthemum and cineraria in vitro | |
| CN106577280A (en) | Rapid propagation aseptic seedling by means of tender stem segments and blades of merrillanthus hainanensis | |
| CN105519434A (en) | A tissue cultured seedling micrografting method for acer palmatum 'orange dream' | |
| JPS63160580A (en) | Culture of callus of crocus genus plant | |
| Umali-Garcia | Tissue culture of some rattan species | |
| Hosseini et al. | High-frequency in vitro direct shoot regeneration from nodal explants of hyssop plant (Hyssopus officinalis L.) | |
| JPH02238829A (en) | Raising seedling of dalmatian pyrethrum | |
| SIN et al. | FROM OIL PALM (ELAEZS GUANEENSS JACO.) CALLUS | |
| JPS6015286B2 (en) | Mass propagation method for lily seedlings | |
| JPS61205427A (en) | Shoot apex culture method of palmate leaf | |
| KR20160046999A (en) | Callus induction medium, shoot induction regeneration medium for Chrysanthemum anther culture, and preparing method of Chrysanthemum haploid plantlet by anther culture using the same | |
| Thakur et al. | In vitro study of callus induction in Wedelia trilobata using different growth regulators | |
| Sharma et al. | Effect of additives on enhanced in vitro shoot multiplication and GC-MS analysis of Lawsonia inermis L | |
| CN108849515A (en) | A kind of culture medium and method of the generation of induction macleaya cordata embryo shape callus | |
| JPH08298887A (en) | Tissue culture of santalum and its secondary proliferation | |
| JP2545359B2 (en) | Plant culture cells | |
| KR20050078372A (en) | Method for production of plantlets and adventitious roots from embryogenic callus of mountain ginseng | |
| KR101963644B1 (en) | Method of Cultivating Youngia denticulate using Plant Tissue Culture Techniques and Cosmetic Composition containing an Extract from Callus or Suspension Culture | |
| CN109874678A (en) | A kind of method for inducing and cultivating of bletilla callus |