JPS63150218A - Stench-preventive and deodorizing agent - Google Patents

Abstract

PURPOSE:To provide the titled stench-preventive and deodorizing agent containing an extract of a specific plant, etc., having antibacterial activity against dermatic indigenous anaerobe, exhibiting excellent effect to suppress body odor such as underarm odor, foot odor, foul breath, etc., and having high safety. CONSTITUTION:A plant of Cupressaceae family belonging to Thujopsis genus (e.g. Thujopsis dolabrata, etc.), Thuja genus (e.g. Thuja standishii, etc.) or Juniperus genus (Juniperus rigida, Juniperus conferta, Juniperus chinensis, Juniperus chinensis var. procumbens, Juniperus chinensis var. sargenti, etc.) is extracted with a solvent, preferably ethanol, propylene glycol, water, hexane, etc., and the extract is used as a component of the objective agent. As an alter native, the leaves or twigs of the above plant is picked and crushed as is or after drying and the crushed product is used as a component. The crushed product is preferably sieved with a sieve passing particles of 2mm in diameter and the fraction passing through the sieve is used as the component. The amount of the extract or crushed product in the stench-preventive and deodorizing agent is preferably 0.001-50wt.% in the whole agent in terms of dried residue.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention contains an extract of a plant belonging to the Cupressaceae family, the genus Asunaro, the genus Arborvitae, or the genus Juniper, or a crushed product of leaves or twigs of the plant. This article relates to a deodorizing deodorant that is highly safe and has the ability to suppress body odors such as armpit odor, foot odor, and bad breath.

[Prior art] Deodorizing technology uses physical effects such as neutralization through chemical reactions, adsorption, and inclusion, or when microorganisms are involved in the odor substances, disinfectants are used, or strong odors are used to eliminate bad odors. There are known methods for masking. For example, using potassium permanganate or chlorine dioxide, hydrogen sulfide, phenol,
There are methods of removing malodorous substances by reacting with ammonia, mercaptan, etc. to turn them into odorless compounds, coexisting with substances with excellent adsorption and inclusion abilities such as activated carbon, zeolite, and cyclodextrin, and methods of removing malodorous substances such as hexachlorophene and trichlorocarbanilide. Generally known methods include adding disinfectants to prevent decomposition by microorganisms, and masking bad odors with strong scents such as peppermint, cinnamon, and orange flavors. These deodorizing techniques are often used alone or in combination depending on various factors such as the malodorous substance, its source, the place of application, safety, and the persistence of the effect.

On the other hand, as deodorant ingredients derived from plants, tannic acid (Japanese Patent Laid-Open No. 54-8725), hydroxycinnamic acid (Japanese Patent Laid-Open No. 54-8725)
l-27882), abietic acid (Japanese Patent Application Laid-open No. 54-117
031), Shikonin (Japanese Unexamined Patent Publication No. 59-80761), etc. have been disclosed.

[Problems to be Solved by the Invention] However, these conventionally used deodorizing techniques include methods that use disinfectants such as benzalkonium chloride and trichlorocarbanilide for the purpose of suppressing microorganisms, and methods that use disinfectants such as benzalkonium chloride and trichlorocarbanilide to suppress bad odors themselves. In methods that use activated carbon, zeolite (Japanese Patent Application Laid-Open No. 6O-178810), etc. for the purpose of adsorbing sterilants, the disinfectant may be inactivated by the components of sebum and sweat, or the adsorbent may become inactive due to the large amount of sebum and sweat. Because the absorption capacity is reduced, the practical deodorization and deodorization effects are often unsatisfactory. In addition, in order to improve the deodorizing effect, it is possible to use a disinfectant in combination with an adsorbent, but the adsorbent, such as the combination of zeolite and benzalkonium chloride, adsorbs the disinfectant and inactivates its effect, reducing its effectiveness. It had drawbacks that offset it.

Moreover, among the plant-derived deodorizing ingredients described above, there are few that can actually be applied to the skin, and most of them cannot be expected to have an aromatic masking effect.

[Problems to be solved by the invention] In view of the above-mentioned circumstances, the present inventors have investigated the antibacterial activity of plants or their extracts in the process of conducting research with the aim of developing ingredients effective in suppressing body odor. As a result of extensive research into the effect of suppressing body odor, we have found that extracts of plants belonging to the Cupressaceae family, Arborvitae genus, or Juniper genus, or crushed leaves or twigs of these plants, have been found to be effective against skin odor, which is said to be the cause of body odor. It has been found that it has antibacterial activity against anaerobic bacteria and has an excellent body odor suppressing effect in practical use. These things are not suggested at all in previous reports,
This was discovered for the first time by the present inventors, and the present inventors completed the present invention based on these findings.

That is, the present invention provides a deodorizing and deodorizing agent containing an extract of a plant belonging to the Cupressaceae family, Arborvitae genus, or Juniper genus, or a crushed product of leaves or twigs of the plant.

The configuration of the present invention will be explained in detail below.

The plants used in the present invention include Cupressaceae, Asunaro genus, Arborvitae genus,
Or a plant belonging to the genus Oriental, specifically, Asunaro, Ate, or Hinokiasunaro of the genus Asunaro, Arborvitae of the genus Arborvitae, Junniper, Juniper, Juniper, Ibuki, Hijakusin, or Juniper of the genus Juniper.

In the present invention, these plants are used in the form of crushed products or extracts. To obtain a pulverized product, leaves or twigs of the above plants are collected and pulverized as is or after drying. There is no particular restriction on the degree of pulverization, but since the finer the pulverization, the better the deodorizing and deodorizing effect, the pulverized product is preferably passed through a comb that can pass particles with a diameter of 2 mm.

In producing the extract, conventional methods for plant extracts are used. That is, leaves or twigs of the above plants are extracted using the solvent described below. Although the ratio of extracted contents of leaves changes somewhat depending on the time of collection, leaves from any time of collection can be used. There are no restrictions on the use of the extraction solvent as long as it is a solvent commonly used for plant extraction. However, depending on the combination of solvents, the ratio of extracted contents may change or the degree of coloring may vary, so ethanol, propylene gelcol, water, hexane, etc. are preferable. Extraction conditions are not particularly limited as long as they are conditions commonly used for plant extraction. However, since the degree of crushing of the leaves affects the extraction time and extraction efficiency, it is preferable to crush the leaves and pass them through a comb that can pass particles with a diameter of 2 mm, and use the crushed material that has passed through the comb. Although the ratio of extracted contents changes depending on the extraction temperature, there is no particular restriction. However, if you also want to obtain aroma components, it is preferable to avoid it as there is a risk of volatilization at high temperatures. The amount of extraction solvent is also not particularly limited, but from the viewpoint of extraction efficiency and ease of post-processing such as filtration, it is most desirable to use 1 part to 10 parts of extraction solvent for 1 part of leaf.

When used as an extract, it may be used as a solution of extracted i:shi, or as a concentrated extract by distilling off the solvent, or as a highly viscous substance or powder with the solvent completely distilled off. May be used.

The deodorizing and deodorizing agent of the present invention preferably contains the extract or pulverized product obtained as described above in an amount of 0.001 to 50% by weight as a dry residue based on the total amount of the deodorant and deodorant. , most preferably from 0.1 to 20% by weight.

In addition to the above-mentioned extract or pulverized product, existing bactericides, deodorants, etc. can be used in the deodorant and deodorizer of the present invention as long as they do not impair the effects of the present invention.

The formulation of the deodorant and deodorizer of the present invention is arbitrary, and may be solid, solution, solubilized, emulsified, powder dispersed, water-oil two-phase, water-oil/powder three-layer, etc. Any dosage form is acceptable.

[Example] Next, the present invention will be explained in more detail with reference to Examples.
It goes without saying that the scope of the present invention is not limited to these examples. In addition, in the following examples, the blending amount is shown in weight %.

Preparation Example 1 Leaves and twigs of Asunaro are crushed in a speed mill, and the diameter is 2
Only the pulverized material that passed through the comb was collected through a comb that could pass through the millimeter particles. Next, 5 parts of ethanol was added to 1 part of the pulverized product, and the mixture was extracted indoors at 20 to 40°C for 4 weeks and filtered through a glass filter to obtain a brown extract.

Preparation Example 2 Ate leaves and twigs were ground in a speed mill to a diameter of 2 mm.
Only the crushed material that passed through the comb was collected through a comb that could pass through the particles. Next, 3 parts of propylene glycol was added to 1 part of the pulverized product, and the mixture was extracted for 3 weeks indoors at 20 to 40°C and filtered through a glass filter to obtain a green extract.

Preparation Example 3 Arborvitae leaves and twigs were air-dried, then pulverized in a speed mill, passed through a comb that could pass particles with a diameter of 2 mm, and only the pulverized material that passed through the comb was collected. Next, 5 parts of n-hexane was added to 1 part of the pulverized product, and the mixture was extracted indoors at 20 to 40°C for 4 weeks and filtered through a glass filter to obtain a green extract. N-hexane was distilled off from the extract to obtain a dark green viscous substance.

Preparation Example 4 Undried hibiscus leaves were pulverized in a speed mill, passed through a comb capable of passing particles with a diameter of Inm, and only the pulverized material that passed through the comb was collected.

Next, the antibacterial activity and deodorizing effect of the plant extract obtained as described above will be described below.

Test Example 1 ABCM medium (Eiken) was used as an antibacterial activity medium, and heat treated in an autoclave at 115°C for 15 minutes.

Soak 0.05ml 1L of 10% acetone solution of each sample into a mouthpiece disc (8mmφ), and prepare Propionibacterium avidum (Propionibacterium avidum).
avidum.

ATCC25577) was allowed to adhere to the agar plate on which it had been inoculated and dispersed, and anaerobically cultured at 37°C for 3 days. J of opening paper at the end of culture
[The diameter of the zona pellucida (Propionibacterium avidum inhibition zone) produced was measured and the antibacterial activity was determined.

The results are shown in Table 1.

(The following is a blank space) Table 1 Extract of Preparation Example 1 18 Extract of Preparation Example 2 20 Extract of Preparation Example 3 28 Test Example 2 Inhibition effect on bacteria isolated from the armpit Bacteria were isolated from the armpit by the stamp method. Aerobic epidermal resident cocci were cultured at 32°C using a broth agar medium (manufactured by Sito Pharmaceutical).
After culturing for a period of time, the colonies that appeared were examined under a microscope to select cocci. 5taphylococcus was identified using the standard identification method.
epidermid, is, Micrococcus
SP, l has finished. At the same time, CAM agar medium (manufactured in Japan) was used to culture at 37°C for 7 days anaerobically.
Nibacterium sp. was isolated.

The inhibitory effect of the extract obtained in Preparation Example 2 by adding the strain stored in our laboratory to these bacteria was determined by the minimum inhibitory concentration (M1
When evaluated using C), the following strong effects were observed.

11 plates of anal poo red 5 taphyl
oeoccus aureus 209P
1000 Staphylococcus epider
midis IFo 3762 1000Staphy
lococcus epidermidis (isolate)
1000Microco100Osp, (isolate) 1100OPropionfbac
terfu avidum (ATCC25577)
300-Propionibacterium sp,
(isolated strain) 300 Test Example 3 Measurement of deodorizing effect on foot odor A person with strong foot odor was asked to wear 100% cotton socks for 10 hours, and only the soles of the feet were cut out and two 2 x 2 cm test pieces were placed.
I prepared 0 pieces. One piece of each flask was placed in a 50 ml Erlenmeyer flask, sealed with a silicone stopper, and divided into 10 sets, each consisting of two flasks. The extract obtained in Preparation Example 2 was added to one Erlenmeyer flask of each set in 0.1d increments from 0.1d to 1tnL, and the solvent used in Preparation Example 2 was added to the other Erlenmeyer flask as a control. That is, 0.1 to 11n of ethanol
It was added in 0.1 m increments up to t and left for 30 minutes. #! After placement, the deodorizing effect was evaluated by five sensory panelists. The results are shown in Table 2.

The evaluation method was as follows.

Rating: 0...I don't feel any foot odor 1...
・I feel a little bit of foot odor 2...I feel some foot odor 3...I feel a lot of foot odor 4...I feel a strong foot odor (blank below) *S... Foot odor sample P...Foot odor control product Test example 4 Measurement of deodorizing effect on armpit odor Five layers of 4 x 4 cm gauze were filled with 0.3 mt of a 10% ethanol solution of the extract obtained in Preparation Example 3. A sample containing 0.3 ml of ethanol was prepared as a test piece. A test piece and a control product were fixed with adhesive tape under both liquids of six panelists suffering from armpit odor, and two hours later, two specialized sensory evaluators evaluated the odor of the test piece and control product. . A self-evaluation was also conducted by six panelists who were concerned about armpit odor. The results are shown in Table 3.

The evaluation method was as follows.

Rating: 0...I don't feel any underarm odor 1...
- I feel a slight odor in my armpits2...I feel an odor in my armpits3...I feel a lot of odor in my armpits4...I feel a strong odor in my armpits (see the margins below)Table 3 Evaluation of deodorizing effect on armpit odor Results *T...Test piece P...Control product Formulation examples of the deodorizing and deodorizing agent according to the present invention are shown below.

Example 1: Deodorant lotion (1) Asunaro extract (Preparation example 1) 2
．． 0(2) Aluminum hydroxychloride 20
．． 0 (3) Polyoxyethylene (20 mol) Oleyl alcohol ether 0.5 (4) Propylene glycol 5.0 (5) Ethyl alcohol 25.51F+ (6) Purified water 47.5
(Manufacturing method) Add (2) and (4) to (6) and dissolve (wood). (5
) and dissolve the remaining (1) and (3) (alcohol part). Add alcohol to the wood to solubilize it and filter.

(Practical use test) A composition was prepared by removing the Asunaro extract from the deodorant lotion of Example 1, and this was used as a control product, and a practical use test was conducted on a panel of 8 people. Each sample was filled in a container with a dispenser and was sprayed directly onto the skin or sprayed onto the skin and then applied to the skin, and the underarm odor 2 hours after application to the skin was evaluated by an expert sensory evaluator.

The results are shown in Table 4.

(The following is a blank space) a [Rating] The deodorant lotion of Example 1 compared to the control product
Very deodorizing effect...3 points Considerably deodorizing effect...2 points Slightly deodorizing effect...
...Has the same deodorizing effect...
0 points Example 2: Deodorant powder (1) Zinc white 3.0 (
2) Ate extract (Preparation example 2) 1.
0(3) Talc 93
．． 0(4) Liquid paraffin 3
．． 0↓ O (Production method) (IL Mix (3) well with a blender, add (4)
) is uniformly sprayed, and then (2) is further uniformly sprayed, then pulverized and compression molded.

Example 3: Deodorant spray plate 5.0 (1) Aluminum hydroxychloride 5.0 (
2) Propylene glycol 5.0 (
3) Isopropyl myristate ester 2.0 (4
) trioleic acid ester 3.0 (5
) Arborvitae extract (Preparation Example 3) 0.1 (
6) Ethanol 84.9 full 1 hour stock solution 35.0 Freon 12 43.0 Freon 11 22.0 (Production method) Add (1) to (6) and dissolve (2), (3), (4),
Add (5), dissolve, and filter. For filling, fill the can with the prescribed amount of stock solution, and after attaching the valve, fill with the prescribed amount of gas.

Example 4: Deodorant stick (1) Zinc white 5.0 (
2) Solid paraffin 5.0 (
3) Sodium palmitate 12.0 (
4) Propylene glycol 20.0 (
5) Ethyl alcohol 50.0 (
6) Ate extract (Preparation example 2) 0.
5(7) Purified water 7.
5 (Production method) Add (3) to (5) and dissolve, then (4), (6), (
After adding and dissolving 7), heat (1) and (2) in advance, mix them with a homomixer to homogeneously disperse them, pour into a mold, and rapidly cool.

Example 5: Deodorant foot spray Rinhogogo (1) Aluminum hydroxychloride 8.0 (
2) Dipropylene glycol 3.0 (
3) Isopropyl myristate ester 2.0 (4
) trioleic acid ester 3.0 (5
) Asunaro extract (Preparation example 1) 5.0
(6) Ethanol 79.0
Mitsukou Onigo stock solution 30.0 Freon 12 45.0 Freon 11 25.0 (Production method) Add (1) to (6) and dissolve, then (2), (3), (4)
, (5) are added, dissolved, and filtered. For filling, fill the can with the prescribed amount of stock solution, and after attaching the valve, fill with the prescribed amount of gas.

Example 6 Deodorant toothpaste (1) Calcium phosphate dihydrate 45.0 (
2) Silicic anhydride 2.0 (3
) Glycerin 10.0 (4)
Sorbitol 10,0(5)
Carboxymethyl cellulose 1.0 (6) Sodium lauryl sulfate 1.81 Di (7) Arborvitae extract (Preparation Example 3) 0.
5(8) Purified water 29.7
(Manufacturing method) Add (5) to (8) and allow to swell and dissolve. Add the other ingredients to this, stir well, and degas.

(Practical use test) A composition obtained by removing the arborvitae extract from the deodorant toothpaste of Example 6 was used as a comparative example.

In the actual use test to measure the deodorizing and deodorizing effect, a panel of 6 people brushed their teeth as usual 30 minutes after lunch for 6 consecutive days. A blank product was used on the first weekday, and the sample of Example 6 was used on the second weekday. The tooth brushing action was left to each panel and no particular restrictions were given.

The deodorizing and deodorizing effect was evaluated daily by a professional sensory evaluator one hour after tooth brushing.

The results are shown in Table 5.

(Leave below) (Rating) I'm concerned about bad breath...
・・・・・・Two points: I’m a little concerned about bad breath.
・・・・・・・・・One point: I don't have to worry about bad breath.
・・・・・・・・・・・・・・・O point Example 7: Deodorant sheet Place the crushed leaves obtained in Preparation Example 4 between two sheets of nonwoven fabric, and apply it evenly per 20 cm + 2 of the nonwoven fabric. A deodorant sheet was obtained in which 1 g of the crushed material was fixed on the surface of a nonwoven fabric using a water-soluble polymer adhesive to prevent leakage, and the periphery of the nonwoven fabric was sealed.

Claims (1)

[Claims] (1) A deodorizing and deodorizing agent containing an extract of a plant belonging to the genus Asunaro, Arborvitae or Juniper of the family Cupressaceae, or crushed leaves or twigs of the plant.