JPS6312966A - Partial injector - Google Patents

Abstract

PURPOSE:To attach a liquid injector as it is which samples a sample liquid, and to directly spot-attach the sample solution of a prescribed quantity onto a reagent layer from the liquid injector, by constituting the titled partial injector of the liquid injector, a fixed supporting base, an operating means, a contact sensor, an up-and-down driving means, an up-and-down motion control means, a placing base, etc. CONSTITUTION:First of all, a sample liquid is sampled by a liquid injector 10, a cylinder 12 is inserted into the supporting hole 21a of a supporting arm 21 by pointing an injection needle 13 downward, and the liquid injector 10 is attached to a fixed supporting base 20. In such state, when a start switch is turned on, both an up-and- down support 25 and an operating arm 30 move downward through a pinion 51 and a rack 25b, the lower face of a left end 30a abuts on the upper end 11a of a piston 11, and this abutting is detected by a contact sensor 35. When a controller receives this detecting signal, the support 25 and the arm 30 are further moved downward by a prescribed quantity, based on a spot-attaching quantity data of the sample liquid. In such way, the piston 11 is also moved downward by a prescribed quantity, and from the upper end 13a of the injection needle 13, the sample liquid of a prescribed quantity is spot-attached and supplied exactly into the reagent layer 1a of a chemical analysis slide 1 on a placing base 40.

Description

Detailed Description of the Invention (Field of Industrial Application) The present invention relates to a dispenser that dispenses a fixed amount of a sample liquid in a sample container onto a chemical analysis slide having a predetermined reagent layer. More specifically, the present invention relates to a dispenser that automatically performs this spot supply.

(Prior Art) Qualitative or quantitative analysis of specific chemical components in a liquid sample is a commonly performed operation in various industrial fields. In particular, quantitative analysis of chemical components or formed components in biological body fluids such as blood and blood is extremely important in the biochemical and clinical fields.

In recent years, dry-type slides have been developed that are capable of quantitatively analyzing specific chemical or organic components contained in a sample solution by applying small droplets of the sample solution. Publication No. 53-21677, JP-A-55-16435
No. 6, etc.) has been put into practical use. These slides can be used to analyze sample liquids more easily and quickly than conventional wet analysis methods, and their use is especially useful in medical institutions and laboratories that need to analyze a large number of samples. etc., it is preferable.

To analyze the chemical components in a sample solution using such a slide, the sample solution is weighed onto the slide and then kept at a constant temperature for a predetermined period of time in an incubator (incubator). The slide is then irradiated with measurement irradiation light containing a wavelength pre-selected based on the combination of the sample components and the reagents contained in the reagent on the slide, and its reflection is detected. It measures optical density, which allows quantitative analysis of the chemical components, etc. mentioned above.

When performing such an analysis, a predetermined amount of the sample liquid to be spotted onto the reagent layer of the slide must be measured and spotted at the required degree. This is because if the amount of the sample liquid differs from the predetermined amount, the reflected optical density will differ and the analysis accuracy will also decrease. For this reason, when performing spot supply of sample liquid,
Various pipettes and the like have been devised so that a predetermined amount can be applied correctly. For example, such a pipette uses a piston-cylinder mechanism to attach a spotting tip to the tip of the pipette, suck a predetermined amount of sample liquid into the spotting tip by pushing and pulling out the piston, and then apply this predetermined amount of sample liquid to the tip. Supply the sample solution by spotting it onto the reagent layer of the slide:
There is something.

(Problem to be Solved by the Invention) To place a sample solution onto the reagent layer of a slide using the above-mentioned spotting pipette, first put the sample solution into a container, and then apply the sample solution in this container. The sample liquid is suctioned and held in the spotting tip at the tip of the pipette, and then the sample liquid is discharged onto the reagent layer of the slide from the tip of the spotting tip. For this reason, it is necessary to put the sample liquid into a container that can be aspirated using a spotting tip. The sample liquid is often human blood, etc. In this case, blood is collected from the human body using a syringe, then discharged into a designated container, and then aspirated using a spotting pipette. The clothes are put on. In this way, when collecting a sample liquid (blood) using a liquid injector, if the sample liquid can be spotted directly from the liquid injector onto the reagent layer of the slide, it will be possible to This has the advantage of not only eliminating the need for a dropper pipette, but also simplifying the spotting operation.

However, when spotting directly onto the reagent layer from the liquid injector, it is difficult to precisely apply a predetermined amount of sample liquid onto the reagent layer by manually operating the liquid injector. There is a problem.

In view of the above-mentioned problems, the present invention has been developed by collecting a sample liquid using a liquid injector, and then attaching the liquid injector directly onto the reagent layer of a chemical analysis slide. The objective is to provide a dispenser that can directly instill a fixed amount of sample liquid from the injector, and as a means for this purpose, the dispenser is configured as follows.

This dispenser includes a liquid injector, a fixed support base that supports the cylinder of the liquid injector with the injection needle facing downward, and a fixed support base that is movable up and down with respect to the fixed support base. an actuating means that extends above the piston of the liquid injector and is moved downwardly so as to contact the upper end of the piston and press down the piston; a contact sensor that detects contact with the piston, and a vertical drive means that controls the vertical movement of the actuating means, and when the contact sensor detects contact between the actuating means and the upper end of the piston, activates the vertical drive means. A chemical device having a vertical movement control means for lowering the means by a predetermined amount vJ to push down the piston by a predetermined amount, and a predetermined reagent 1 below the syringe needle attached to a liquid injector whose cylinder is fixedly held by a fixed support base. It consists of a mounting table on which the analysis slide is placed.

The actuating means includes a vertical column that is supported vertically movably with respect to the fixed support base and is moved up and down by a vertical drive means, and an actuating device that is fixedly attached to the vertical column and extends above the piston of the liquid injector. The contact sensor may be attached to the operating arm.

(Function) When the dispenser is configured as described above, first a sample liquid such as blood is collected using the injector, and then this injector is attached to a fixed support base, and then the injector is moved up and down controlled by the up and down movement control means. When the actuating means is moved downward by the actuation of the means, and the contact sensor detects that the actuating means has come into contact with the piston of the injector, the actuating means is roughly moved downward by a predetermined amount from this position to move the piston. By pressing down by a predetermined amount, a predetermined amount of sample liquid can be discharged from the injection needle and deposited onto the reagent layer of the chemical analysis slide on the mounting table.

(Example) Hereinafter, an example of the present invention will be described based on the drawings.

FIG. 1 is a front view showing one example of a dispenser according to the present invention, and this dispenser includes a liquid injector 10, a fixed support stand 20, an upper and lower support column 25
, an actuating arm 30, a contact sensor 35, a vi mounting table 40, and a pulse motor 50 for vertical driving.

Note that the upper and lower supports 25 and the actuating arm 30 constitute an actuating means. The liquid injector 10 includes one end 12 of a cylinder 12.
A injection needle 13 is removably attached to the cylinder 12, and the piston 1 is placed in a cylinder chamber 12b that is open at the other end of the cylinder 12.
1 is inserted into the cylinder chamber 12b, and by moving the piston 11 within the cylinder chamber 12b, the sample liquid can be inhaled and discharged from the injection needle 13. The cylinder 12 of this liquid injector 10 is a support arm that protrudes to the side of the fixed support base 20? The injection needle 13 is inserted into and held in the support hole 21a of the injection needle 1, and at this time, the injection needle 13 is directed downward and the piston 11 is held so as to protrude upward.

On the other hand, the upper and lower columns 25 are supported by bearings 21 and 22 attached to the fixed support base 20, and the fixed support base 20
It is mounted so that it can move up and down. This upper and lower support 2
A rack 25b is formed in the center of the rack 2.
5b is a pinion 51 mounted on the rotating shaft of the vertical drive pulse motor 50 fixedly held on the fixed support base 20.
I'm meshing with it. Therefore, when the pulse motor 50 is rotationally driven, the pinion 51 is rotationally driven and the rack 25b is rotated.
Is it possible to move up and down the up and down support? 5 is moved up and down. On the other hand, an operating arm 30 is fixed to the upper end of the upper and lower columns 25, and this operating arm 30 extends to the right in the figure. Located above. A contact sensor 35 is attached to the lower surface of the right end 30a of the actuating arm 30. Therefore, the upper and lower support columns 25 are moved down, and the actuating arm 30 is moved downward, so that the lower surface of the right end 30a of this arm 30 is the upper end of the piston 11. When this contact occurs, the contact sensor 35 can detect this contact.

Further, a mounting table 40 is disposed below the liquid injector 10 supported by the fixed support table 20, and this mounting table 40 has a recess 41 for holding the chemical analysis slide 1.
The chemical analysis slide 1 is held so that the center of the reagent @la on the chemical analysis slide 1 is located directly below the injection needle 13.

The operation of the vertical drive pulse motor 50 is controlled by a controller 60 as vertical movement control means, and the control system by this controller 60 will be explained with reference to FIG. The controller 60 receives a signal from the contact sensor 35, a signal from a keyboard 62 (not shown in FIG. 1) provided on the dispenser, and a start switch 63 (not shown in FIG. 1) provided on the dispenser. Based on these input signals, a predetermined output signal is output to the driver 61, and the driver 61 drives the vertical drive pulse motor 50 in accordance with this output signal. let

Note that the start switch 63 is a switch for starting control by the controller 60, and is a switch for starting control by the controller 60.
is for inputting data such as the amount of sample liquid spotted and the number of times it was spotted.

The operation of depositing a sample liquid using the dispenser configured as described above will be explained. First, a sample liquid such as human blood is collected using the liquid injector 10. The amount to be collected at this time only needs to be more than the amount required for spotting, and there is no need to detect the exact amount.

Then, as shown in FIG. , insert the cylinder 12 into the support hole 21a of the support arm 21 with the injection needle 13 facing downward, and attach the liquid injector 10 to the fixed support base 20.

After that, data such as dot @ amount and number of dots are inputted from the keyboard 62, and the start switch 63 is turned on.8: Based on this input, the controller 60 controls the driver 61
The pulse motor 51 is rotationally driven via the pinion 5.
1 and the rack 25b, the vertical support 25 is moved down. At this point, the actuation arm 30 fixed to the upper end of the upper and lower columns 25 is located above the piston 11;
When the vertical column 25 is moved downward, the operating arm 30 is also moved downward, and the lower surface of its right end 30a finally comes into contact with the upper end 11a of the piston 11. This contact is detected by the contact sensor 35, and the detection signal is sent to the controller 6.
Sent to 0. When the controller 60 receives this detection signal @, it rotates the pulse motor 51 a predetermined amount based on the sample liquid spotting amount data inputted from the keyboard 62, and roughly moves the upper and lower columns 25 and the actuating arm 30 downward by a predetermined amount. let

As a result, the actuating arm 30 pushes down and the piston 11 is also moved down by a predetermined amount, but this downward movement is caused by the downward movement of the piston 11.
This amount is such that the amount of sample liquid discharged from a is equal to the dotted amount inputted from the keyboard 62.

As a result, a predetermined amount of sample liquid is transferred from the lower end 13a of the injection needle 13 to the chemical analysis slide placed on the mounting table 40.
is precisely spotted and supplied onto the reagent layer 1a.

In order to perform continuous dotting, after replacing the chemical analysis slide 1 on the mounting table 40, press the start button again to drive the pulse motor 50 again and move the piston 11.
All you have to do is move it down by a predetermined amount. This point@amount can also be changed each time the point is placed by changing the input data on the keyboard 62.

(Effects of the Invention) As explained above, according to the present invention, after collecting a sample liquid such as blood using a liquid injector, a chemical analysis slide can be prepared by simply setting the liquid injector in a dispenser. A predetermined amount of sample solution can be accurately spotted onto the reagent, and
The amount of this sample liquid applied can also be set arbitrarily. Therefore, there is no need to use a separate container for storing the sample liquid or a spotting pipette as in the past, and the spotting operation becomes easier.

[Brief explanation of the drawing]

FIG. 1 is a front view showing an example of a dispenser according to the present invention, and FIG. 2 is a block diagram showing a drive control system for a vertical drive pulse motor constituting the above-mentioned spotting table. 10... Liquid injector 11... Piston 12
...Cylinder 13...Syringe needle 20...
Fixed support base 25... Upper and lower supports 30... Operating arm 35... Contact sensor 40... Mounting base

Claims (1)

[Scope of Claims] 1) A liquid injector consisting of a cylinder having an injection needle or injection tip removably attached to one end, and a piston slidably fitted into a cylinder chamber opened at the other end of the cylinder; a fixed support that removably supports the cylinder of the liquid injector with the one end facing downward; and a fixed support that extends above the piston of the liquid injector that is attached with the cylinder supported by the fixed support. an actuating means that is movable up and down with respect to the base and can be moved downward to abut the upper end of the piston and push the piston down; a contact sensor for detecting contact; a vertical drive means for vertically moving the actuating means; and a vertical drive means for controlling the vertical movement of the actuating means; and a contact between the actuating means and the upper end of the piston by the contact sensor. a vertical movement control means that operates the vertical drive means to move the actuating means downward by a predetermined amount to push down the piston by a predetermined amount via the actuating means; A dispensing device comprising a mounting table on which a chemical analysis slide is placed below the injection needle or injection tip attached to a supported liquid injector. 2) The actuating means includes upper and lower struts that are supported movably up and down with respect to the fixed support base, and is fixed to the upper and lower struts and extends above the piston of the liquid injector, and is movable downwardly together with the upper and lower struts. and an actuating arm that is capable of contacting the upper end of the piston and pushing the piston down, and the vertical support is moved up and down by the vertical drive means,
A dispenser according to claim 1, characterized in that said contact sensor is attached to said actuating arm.