JPS63123370A - Health drink - Google Patents

Abstract

PURPOSE:To obtain the titled drink having drug effect, e.g. hypoglycemic property, etc., without coloring even in the presence of water, by adding an aloe extract prepared by treating a crude aloe extract with a fat-soluble organic solvent to an alcoholic drink or an aqueous drink containing vinegar. CONSTITUTION:A drink obtained by initially removing epidermis of leaf parts of aloe to collect an inner jelly part, drying, pulverizing and treating the inner jelly part with water, filtering the treated inner jelly part to give a filtrate, concentrating (drying) the resultant filtrate to provide a crude aloe extract, then treating the crude aloe extract with a fat-soluble organic solvent in an amount of 3-8 times based on the extract to form an aloe extract and adding the aloe extract to an alcoholic drink, e.g. 'SHOCHU' (low-class distilled spirit), refined 'SAKE' (Japanese rice wine), etc., or aqueous drink containing vinegar, e.g. brewed vinegar, etc. The raw material plant for the above-mentioned aloe extract is preferably aloe species having large leaves.

Description

[Detailed Description of the Invention] (a) Field of Industrial Application This invention relates to health drinks, and in particular, to alcoholic drinks or aqueous drinks containing vinegar, the crude aloe extract treated with a fat-soluble organic solvent is added. About health drinks.

(b) Conventional techniques and problems Aloe, which belongs to the Liliaceae family, contains a substance composed of polysaccharides that has a hypoglycemic effect.
It is known that As a result of various researches, the inventor of this invention found that, for example, Aloe vera (Aloe barbadensis M.
For example, by drying and pulverizing the jelly-like part of the leaves of ), treating the powder obtained with water and drying it,
A crude extract of aloe having the above-mentioned effects was obtained. However, this crude extract not only turns brown in the presence of water, but also is hygroscopic, so the ingredients serve as nutrients and microorganisms proliferate in large quantities, with the number of viable bacteria per y being on the order of 106. Also, the coliform bacteria detection test was positive for gas.

Therefore, it is inconvenient to use this crude extract as it is by adding it to a plant such as Ichikina.

The above fungi can be sterilized using the usual heat sterilization method, for example, by treating at 170 to 180°C under a pressure of 2 kg/al; however, the crude extract itself not only turns brown, but also contains polysaccharides. It also changes in quality. Also, the so-called low temperature sterilization method at about 70 to 80 degrees Celsius is insufficient for sterilization.

(c) Means for Solving the Problems In order to solve the above-mentioned problems, the inventor of this invention has conducted extensive research and found that when treated with a fat-soluble organic solvent, the crude extract of aloe can be used without impairing its medicinal properties. This method was developed based on the discovery that it does not discolor even in the presence of aloe vera and achieves a practically acceptable number of viable bacteria per unit weight. The present invention provides a health drink in which an aloe extract obtained by treatment with a soluble organic solvent is added in the following amount to be dissolved in the drink.

The plant material used in this invention is Liliaceae (Liliaceae).
It is an aloe belonging to the family (Eae), and its leaves are used. Those with large leaves and a large amount of jelly inside are preferable, and examples include the following.

Aloe barbadensis Miller, also called Aloe Vera
): Aloe arborecens mirror (Alas arbore!+cens) called Kidachi Aloe
gill, ) Near 0 Engineering Ferox Mill (Aloe ferox Mill, ) 27020 Africana Mirror (Aloe african)
a Mill,) HI3 Engineering Peri” Baker (Al
oe perr)'i Baker): Aloe Puricateilis
) Near 0 engineering Φ saponari 7 (Aloe 5apona
ria Haw).

The aloe extract used in this invention can be produced as follows.

The internal jelly-like part of the aloe leaf was collected by removing the epidermis as described above. 1) The powder obtained by drying and pulverizing was treated with water, and the obtained liquid was concentrated or concentrated. A crude aloe extract can be obtained by drying or 11) concentrating or condensing and drying the resulting liquid through compression filtration.

The above concentration or condensation drying is preferably carried out under reduced pressure at a temperature not exceeding about 60°C, but freeze drying may also be performed. Further, the step of treating with water in 1) above is carried out, for example, by mixing and stirring the produced powder and water, but the temperature in this case may be room temperature.

However, the temperature may be slightly increased, and in this case, the process time can be shortened.

Next, the crude aloe extract obtained by straining as described above is treated with about 8 to 8 times the strength of the following fat-soluble organic solvent.

The treatment is carried out, for example, by mixing and stirring the two, preferably separating them in a furnace, and removing the organic solvent from the furnace solids by a conventional method (
For example, by vacuum evaporation or decanting), the aloe extract used in this invention is obtained as a white powder.

The fat-soluble organic solvents used in this invention include lower alkyl alcohols such as benzene, ethyl acetate, chloroform, n-hexane, methanol or ethanol, lower alkyl ethers such as dimethyl or diethyl ether, and dimethyl or diethyl ketone. Examples include lower alkyl ketones.

When performing the above-mentioned 1111-soluble organic solvent treatment, the crude aloe extract may be in the form of either mucus or powder, but the powder has a smaller volume and contains a smaller amount of
It not only can be processed with solvents, but also has advantages in storage and transportation.

The aloe extract obtained as described above is hygroscopic and is stored in a sterilized airtight container immediately after production.

In addition, this aloe extract has hypoglycemic properties, is soluble in water, exhibits a pale yellow-red color in a mixture of 2% phenol aqueous solution and slight sulfuric acid, and is positive in the silver mirror reaction and Fehling's test solution in the presence of water. It is not colored, has a low number of viable bacteria, and is virtually free of coliform bacteria.

By adding this aloe extract to alcoholic beverages (shochu, sake, whiskey, etc.) or vinegar-containing aqueous drinks (brewed vinegar, black vinegar, etc.) in an amount below its solubility, the health benefits of this invention have a hypoglycemic effect. A drink is obtained. Further, there is no particular limitation on the method of addition, and it may be added at an appropriate step during the manufacturing process of the above-mentioned beverage.

B) Examples This invention will be explained by examples, but the invention is not limited thereto.

Remove the epidermis of mature Aloe barbadensis miller leaves and remove the inner gelatinous part of about 50°F, spread it out and dry it under reduced pressure at a temperature not exceeding 60°C to make about 5g. A dry piece of was obtained.

The dried pieces were ground into a powder of approximately 50 to 100 meshes using a pulverizer. The entire amount of the obtained powder was poured into about 500 ml of distilled water, stirred for about 30 minutes, filtered, and the filtrate was heated to about 60°C.
The crude extract was concentrated and dried at a temperature not exceeding 4.5 g to obtain about 4.5 g of a pale brown powder crude extract. The entire amount of this powder was added to about 6 times the amount of benzene, stirred at room temperature for about 2 hours, and filtered. The resulting residue was treated under reduced pressure at a temperature not exceeding 60°C to remove the solvent, yielding an aloe extract (4, Of, white powder).

Production example of aloe extract 2 Approximately 1 leaf part of X-ripe Alonii vulpadensis miller
After removing the epidermis of the kti and extracting the internal jelly part of about 50°F, this was put into a cotton bag and compressed with a compressor. The resulting mucus was concentrated under reduced pressure at a temperature not exceeding 60'C. After drying, a pale brown powder crude extract of 4.81 was obtained. The entire amount of this powder was added to about 5 times the amount of ethyl acetate, stirred at room temperature for about 2 hours, and then separated. The obtained residue was heated at 60°C under reduced pressure.
The solvent is removed by treatment at a temperature not exceeding 1. A white powder is obtained.3.
71 aloe extracts were obtained.

Mature Aloe alboretscens mirror (Aloe
arborescens B111l, ), remove about 1 liter of epidermis from the leaves, and remove about 150 liters of the inner jelly-like part.
It was taken out, spread out and dried under reduced pressure at a temperature not exceeding 60'C to obtain about 1.6 g of dry pieces. The dried pieces were milled into a powder of about 50 to 100 meshes. The entire amount of the obtained powder was poured into about 200 ml of distilled water, stirred for about 80 minutes, and the filtered liquid was concentrated and dried at a temperature not exceeding about 60°C to obtain about 1.4 g of light brown powder. A crude extract was obtained. The entire amount of this powder was added to about 5 times the amount of benzene, stirred at room temperature for about 2 hours, and filtered. treating the resulting residue under reduced pressure at a temperature not exceeding 60°C to remove the solvent;
Aloe extract (1,2F, white powder) was obtained.

The aloe extracts obtained in Production Examples 1 to 8 have the following properties.

1) Solubility Soluble in water, insoluble in benzene, ether, chloroform, alcohol and acetone.

11) Color reaction A pale yellow-red color is produced with a 2% aqueous phenol solution/concentrated sulfuric acid mixture, and it is positive for the silver mirror reaction and Fehling's reagent.

111) No coloration in the presence of water.

A 0.5% aqueous solution of aloe extract was clear and colorless, but did not become colored when left alone. On the other hand, when the crude extract was left to stand L'''C in the same manner, it turned red.

1v) General viable bacteria count and coliform group Sterile physiological saline solution of aloe extract obtained in Production Example 1 (0
, 5W/V%) was prepared and measured as a sample. A sample without solvent treatment in Production Example 1 was shown as a control.

General viable cell count: Measured by culturing at 81°C for 172 hours using the plate pour method using 80DLP medium according to a conventional method.

Coliform bacteria: Due to gas production and yellowing caused by lactose broth (in Durham tube).

No solvent treatment 2.6X106 Gas positive Benzene treatment 8.5X108 Negative The same test was conducted on the aloe extract obtained in Production Example 2, and the following results were obtained.

No solvent treatment 2.8 x 10 Gas positive Ethyl acetate treatment 4.2 Large intestine 1
It is able to sufficiently cause disease according to the Ministry of Health and Welfare's finger standards (general population number of 8,000 cells/l or less; negative for large P&J bacterial group gas).

In addition, although the aloe extract obtained in the above production example shows hygroscopicity, if it is sealed in a sterilized container in the presence of a dry fish agent and left open for 1 hour, it will remain stable for a long period of time without the growth of general parasites or large intestine group 1. You can do it in a stupor.

(2) Hypoglycemic property The hypoglycemic property of the aloe extract of the above production example was measured by the following method.

Groups of 5 mice (8td, ddY system group 25-801) were created, and blood was collected from the fundus vein of each mouse using a hematocrit tube.
Centrifuge for minutes to obtain plasma. The amount of glucose in the plasma was measured using a glucose analyzer (Yatron M-7000.
(manufactured by Yatron), and the blood sugar level was determined as the non-administration (Ohr) blood glucose level. Immediately after the above blood collection, aloe extract dissolved in physiological saline is intraperitoneally administered.

Blood was collected 7 hours and 24 hours after administration of the sample, and the amount of glucose in the plasma was measured.The relative value when the Ohr blood sugar level was taken as 100 was determined, and the relative blood sugar level was shown in Table 1. The results were expressed as the average soil standard error value, and significant differences were determined by one-dimensional analysis of variance.

Notes to Table 1 Hayashi: P<0.01. Benefit: P<0.001 From the above results, it is clear that aloe vera and aloe have excellent hypoglycemic effects.

Actual Seed Example 1 O and IW/V% of the aloe extracts of Production Example 1 and Production Example 2 were added to the following black vinegar, stirred, and left at room temperature for 6 months, but there was no change in color tone or insolubility. No material was observed.

Yoshi no Sato C Brewed Vinegar) Busshoyama-cho, Takamatsu City (manufactured by Co., Ltd.) Raw Materials: Rice, Alcohol, Hydrochloric Acid Bt: 4.2% Solubility of Aloe Extract of Preparation Example 1 in the above Kuro Vinegar is 0.181W measured by the following method.
/V% values were obtained.

Weigh out 1f of the aloe extract obtained in Production Example 1, put it in an 8-sided flask, add 100 g of the above black vinegar, shake it for 1 hour at 150 times/min using a horizontal reciprocating shaker, and then - Leave it on day and night. Transfer 10 ml of the supernatant liquid to an evaporating dish and dry it on a hot water bath. It was further dried in a constant temperature dryer at 105°C for 6 hours, cooled and weighed to obtain the extract amount A (1/10 d
) was measured and the following measured values were obtained.

0.6690 f/ 10ys1 0.6788 s 0.6714 // Average value 0.6712 // Next, take the above [10t7] in an evaporating dish, dry it on a hot water bath, and then dry it in a constant temperature dryer at 105°C. After drying for 6 hours and cooling, the extract amount B (f/10gt) of the vinegar was measured and the following constant value was obtained.

0.6551 f/ 10yt1 0.6612 // Average value 0.6581 tt The solubility of aloe extract in vinegar was calculated from A and B above as follows.

Mu-B=0.6712-0.6581=0.01811
F/10+++,? =0.181W/V% Example 2 3J4 and 5.15.25.80 and 40 below
% of the aloe extract of Production Examples 1 and 2 to each of the ethanol aqueous solutions. IW/V% was added, stirred and dissolved, and left at room temperature for 6 months, but no change in color tone or generation of insoluble matter was observed.

Examples of shochu 1) Sudachi chu Nakamaeyocho, Tokushima City (Su+HvJA
) 5-1-8 Nisshin Shurui Co., Ltd. Alcohol content 20% Extract content 2% or more and less than 5% 2) Brown sugar shochu 1194 Kamezu, Tokunoshima-cho, Oshima-gun, Kagoshima Prefecture Amami Shurui Co., Ltd. Ingredients: Brown sugar, rice koji Alcohol content = Water and 5.15.
25. The solubility in 30 and 40% dry tanol aqueous solutions was measured as follows.

Weigh out the aloe extract 1f obtained in Production Example 1, put it in an 8-sided flask, add 100 - to each of the above liquids, shake it for 1 hour at 160 times/min using a horizontal reciprocating shaker, and then - Leave it on day and night. Transfer 10W, t of supernatant liquid to an evaporating dish and dry on a hot water bath. 6. In a dryer at a constant temperature of 106°C.
The solubility was measured by drying for a period of time, cooling, and weighing (average value of 8 times).

Solubility water 0.642W/V%55X
- Tanol aqueous solution 0.790//15% 〃0
．． 749// 25% ” 0.729〃 80% // 0.72011 40% N O, 722N (e) Effects of the invention According to the invention, no coloring or insoluble matter is produced, and the number of viable bacteria is practical. It is possible to obtain a health drink that has medicinal effects such as hypoglycemic properties and has no above-mentioned problems.

B1.゛1, - Kochin' stone

Claims (1)

[Claims] 1. A health drink made by adding aloe extract obtained by treating a crude extract of aloe with a fat-soluble organic solvent to an alcoholic beverage or an aqueous beverage containing vinegar in an amount equal to or less than that dissolved in the beverage. Beverages. 2. The health drink according to claim 1, wherein the raw material plant for the aloe extract is an aloe plant with large leaves. 3. The raw material plant for aloe extract is Aloe barbadensis Mi.
ll. ) or Aloe Arborescens Miller (
Aloe arborescens Mill. ) The health drink according to claim 2. 4. The health drink according to claim 1, wherein the fat-soluble organic solvent is one or a mixture of benzene, ethyl acetate, chloroform, n-hexane, lower alkyl alcohol, lower alkyl ether, and alkyl ketone.