JPS6262291B2 - - Google Patents
Info
- Publication number
- JPS6262291B2 JPS6262291B2 JP54067797A JP6779779A JPS6262291B2 JP S6262291 B2 JPS6262291 B2 JP S6262291B2 JP 54067797 A JP54067797 A JP 54067797A JP 6779779 A JP6779779 A JP 6779779A JP S6262291 B2 JPS6262291 B2 JP S6262291B2
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- blood
- antibodies
- absorbance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000427 antigen Substances 0.000 claims description 65
- 102000036639 antigens Human genes 0.000 claims description 64
- 108091007433 antigens Proteins 0.000 claims description 64
- 210000004369 blood Anatomy 0.000 claims description 40
- 239000008280 blood Substances 0.000 claims description 40
- 238000000034 method Methods 0.000 claims description 38
- 239000000523 sample Substances 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 16
- 239000011541 reaction mixture Substances 0.000 claims description 14
- 239000012488 sample solution Substances 0.000 claims description 9
- 210000000601 blood cell Anatomy 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 5
- 230000001678 irradiating effect Effects 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 3
- 239000000969 carrier Substances 0.000 claims description 2
- 210000002700 urine Anatomy 0.000 claims description 2
- 238000002835 absorbance Methods 0.000 description 34
- 239000002245 particle Substances 0.000 description 21
- 229940027941 immunoglobulin g Drugs 0.000 description 17
- 239000004816 latex Substances 0.000 description 17
- 229920000126 latex Polymers 0.000 description 17
- 239000003153 chemical reaction reagent Substances 0.000 description 12
- 229940099472 immunoglobulin a Drugs 0.000 description 12
- 238000005259 measurement Methods 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- 102100023635 Alpha-fetoprotein Human genes 0.000 description 8
- 108010026331 alpha-Fetoproteins Proteins 0.000 description 8
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 230000000984 immunochemical effect Effects 0.000 description 5
- 238000011002 quantification Methods 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 230000000951 immunodiffusion Effects 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 108060003951 Immunoglobulin Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000012472 biological sample Substances 0.000 description 3
- 238000005534 hematocrit Methods 0.000 description 3
- 102000018358 immunoglobulin Human genes 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 230000003169 placental effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 2
- 108010049003 Fibrinogen Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 102000006771 Gonadotropins Human genes 0.000 description 2
- 108010086677 Gonadotropins Proteins 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 229940012952 fibrinogen Drugs 0.000 description 2
- 239000002622 gonadotropin Substances 0.000 description 2
- 230000035931 haemagglutination Effects 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229920000620 organic polymer Polymers 0.000 description 2
- -1 pollen Substances 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- PROQIPRRNZUXQM-UHFFFAOYSA-N (16alpha,17betaOH)-Estra-1,3,5(10)-triene-3,16,17-triol Natural products OC1=CC=C2C3CCC(C)(C(C(O)C4)O)C4C3CCC2=C1 PROQIPRRNZUXQM-UHFFFAOYSA-N 0.000 description 1
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 241001505895 Minuartia marcescens Species 0.000 description 1
- 241000606701 Rickettsia Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 102000011923 Thyrotropin Human genes 0.000 description 1
- 108010061174 Thyrotropin Proteins 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000007822 coupling agent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 210000000624 ear auricle Anatomy 0.000 description 1
- 238000007720 emulsion polymerization reaction Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- PROQIPRRNZUXQM-ZXXIGWHRSA-N estriol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H]([C@H](O)C4)O)[C@@H]4[C@@H]3CCC2=C1 PROQIPRRNZUXQM-ZXXIGWHRSA-N 0.000 description 1
- 229960001348 estriol Drugs 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000001235 sensitizing effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 229920003048 styrene butadiene rubber Polymers 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229960003604 testosterone Drugs 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6779779A JPS55159157A (en) | 1979-05-31 | 1979-05-31 | Quantitative determining method of antigen or antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6779779A JPS55159157A (en) | 1979-05-31 | 1979-05-31 | Quantitative determining method of antigen or antibody |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS55159157A JPS55159157A (en) | 1980-12-11 |
| JPS6262291B2 true JPS6262291B2 (ru) | 1987-12-25 |
Family
ID=13355295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6779779A Granted JPS55159157A (en) | 1979-05-31 | 1979-05-31 | Quantitative determining method of antigen or antibody |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS55159157A (ru) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013042524A1 (ja) | 2011-09-20 | 2013-03-28 | 株式会社日立ハイテクノロジーズ | 自動分析装置及び分析方法 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5964277U (ja) * | 1982-10-21 | 1984-04-27 | 株式会社大洋発條製作所 | 事務用クリツプの自動挾み付け器 |
| US5027994A (en) * | 1989-08-17 | 1991-07-02 | Yong Woo Lee | Stapler combined with a clip driver |
| US5198369A (en) * | 1990-04-25 | 1993-03-30 | Canon Kabushiki Kaisha | Sample measuring method using agglomeration reaction of microcarriers |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5362826A (en) * | 1976-11-10 | 1978-06-05 | Hoffmann La Roche | Quantitative determination of immunologically active substance in analysing sample |
-
1979
- 1979-05-31 JP JP6779779A patent/JPS55159157A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5362826A (en) * | 1976-11-10 | 1978-06-05 | Hoffmann La Roche | Quantitative determination of immunologically active substance in analysing sample |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013042524A1 (ja) | 2011-09-20 | 2013-03-28 | 株式会社日立ハイテクノロジーズ | 自動分析装置及び分析方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS55159157A (en) | 1980-12-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4118192A (en) | Method and apparatus for the measurement of antigens and antibodies | |
| US4208185A (en) | Method and apparatus for the measurement of antigens and antibodies | |
| JP3249919B2 (ja) | 免疫測定方法 | |
| US4521521A (en) | Particle reagent size distribution measurements for immunoassay | |
| US4313929A (en) | Method of measurement of antigens and antibodies | |
| US4203724A (en) | Method and apparatus for the measurement of antigens and antibodies | |
| JP5738328B2 (ja) | 凝集試薬の製造方法、および分析対象物測定方法、ならびに試験キットおよび分析デバイス | |
| KR100894947B1 (ko) | 측정 시약용 담체 입자 라텍스 및 측정 시약 | |
| NL8003294A (nl) | Werkwijze voor het bepalen van een antigeen, antilichaam of antigeenantilichaamcomplex alsmede daarvoor bestemde meetkit. | |
| WO2006025401A1 (ja) | 抗原の測定方法及びそのための試薬 | |
| JPS6365369A (ja) | 抗原−抗体反応の測定法 | |
| JP2005283250A (ja) | 金コロイド凝集反応の測定方法 | |
| JPS6262291B2 (ru) | ||
| JP3779885B2 (ja) | 免疫測定方法 | |
| JP5831448B2 (ja) | 試料中の分析対象物質を測定するための検査器具及びそれを用いた分析対象物質の測定方法 | |
| US20140011190A1 (en) | Method for performing a rapid test | |
| JPS6281567A (ja) | 粒子凝集反応を用いる定量方法 | |
| JPH026023B2 (ru) | ||
| GB1600139A (en) | Method and apparatus for the measurement of antigens and antibodies | |
| GB1600069A (en) | Method for the measurement of antigens and antibodies | |
| JP3618797B2 (ja) | 免疫測定法 | |
| JPH0448265A (ja) | 免疫測定法 | |
| JPS6281566A (ja) | 微粒子の螢光強度測定による定量方法 | |
| JPH01158354A (ja) | 免疫学的多成分測定用試薬と測定方法 | |
| JP2006105910A (ja) | 標的物質の測定方法および測定試薬 |