JPS6247845B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a preventive and therapeutic agent for fat necrosis in cattle.

Cases of fat necrosis in cattle have been reported both domestically and internationally for a long time, and mainly the fatty tissue in the abdominal cavity becomes a mass, putting pressure on the gastrointestinal tract, uterus, kidneys, etc., resulting in disorders, loss of appetite, and constipation. Symptoms such as
It is a deadly disease. It is often found in the serous fat of the kidney, colon, and rectum, and the size and shape of the tumor vary. The reason for this is thought to be due to factors such as the excessive feeding of concentrated feed and lack of roughage in recent cattle breeding.

Current treatments for this disease include the administration of pearl barley, but it is still far from perfect.
A highly effective drug was desperately needed.

Under these circumstances, the present inventors conducted intensive research on fat necrosis in cattle and found that β-sitosterol, γ-sitosterol, brassicasterol,
Stigmasterol, campesterol and γ-
The present inventors have discovered that a crude or purified product containing one or more sterols selected from the group consisting of sitostanol has therapeutic and preventive effects on fat necrosis in cattle, leading to the present invention.

Among the above-mentioned sterols, for example, stigmasterol comes from soybean, palm, cottonseed, kidney bean, omodaka, etc., and campesterol comes from rapeseed, wheat, etc.
Beta-sitosterol is widely found in grains, bark, leaves, etc., as well as in higher plants, such as in the free state, glycosides, fatty acid esters, etc. from soybean, rye, etc., and gamma-sitosterol is found in peas, Calabar beans, rice, etc. Crude plant sterols can be obtained from soybeans, etc., but may also be crude plant sterols obtained by separating oil from these plants, or natural vitamin E, which is touted as a health food, can be obtained from these oils. Crude plant sterols and the like can be provided at low prices, which is a great economic advantage.

The therapeutic agent for preventing and treating bovine fat necrosis of the present invention can be prepared by mixing a crude product or a purified product containing sterol with a physiologically harmless solid or liquid diluent, or by mixing it with a physiologically harmless solid or liquid diluent. Although it may be formulated into granules, suspensions, oils, etc., it is usually mixed directly with conventional cattle feed ingredients, or dispersed in a diluent and added to the above cattle feed ingredients. It is used by

Other appropriate adjuvants or additives, bactericides, preservatives, enzymes, antibiotics, lactic acid bacteria preparations, etc. can also be used in combination.

The amount of the active ingredient used in the therapeutic agent for preventing and treating fat necrosis in cattle of the present invention varies depending on the breed, weight, age, sex, period of administration, type of sterol, etc. of the cattle;
0.5 to 60 g per head per day, preferably 1 to 30 g
Fat necrosis in cattle can be treated by administering 0.05 to 6 g per adult cow per day, preferably
Fat necrosis in cattle can be prevented by administering 0.1 to 3 g.

The agent for preventing and treating bovine fat necrosis of the present invention is further characterized by low toxicity.

Furthermore, the therapeutic agent for preventing and treating fat necrosis in cattle of the present invention has a surprising feature in that it can shrink and eliminate lipomas that have swelled and hardened to the size of a fist or a human head, which occur in the body of a cattle.

Next, the present invention will be explained in detail with reference to Examples and Test Examples.

Example 1 β-sitosterol 3 parts by weight Corncob meal 65 parts by weight Starch 32 parts by weight were mixed uniformly and prepared.

Example 2 Campesterol 2 parts by weight Stigmasterol 1 part by weight Corncob meal 65 parts by weight Defatted soy flour 32 parts by weight were mixed uniformly and prepared.

Example 3 Crude plant sterols (obtained during cottonseed oil production) 20 parts by weight Defatted soybean flour 60 parts by weight Yeast 20 parts by weight were uniformly mixed and prepared into pills.

Example 4 Crude plant sterols (obtained during soybean oil production).
(Contains approximately 15% as plant sterols) 20 parts by weight Corn flour 60 parts by weight Talc 20 parts by weight were uniformly mixed and prepared into granules.

Example 5 Crude plant sterol (obtained when vitamin E was separated from the crude plant sterol used in Example 4. Contains about 60% as plant sterol)
5 parts by weight corncob meal 80 parts by weight starch 15 parts by weight were uniformly mixed to prepare a mixture.

Example 6 β-sitosterol 2.0 parts by weight Brassicasterol 0.4 parts by weight Campesterol 1.2 parts by weight Stigmasterol 0.4 parts by weight Corncob meal 66 parts by weight Starch 30 parts by weight were mixed uniformly and prepared into granules.

Test Example 1 A test was conducted on 12 Japanese Black female cows that were diagnosed with fat necrosis by palpation during breeding or fattening.

A predetermined amount of the bovine fat necrosis prevention and treatment agent of the present invention was mixed with feed and administered daily, and the progress was observed.

Test example 1-1 5 year old female. The symptom was a fist-sized mass around the rectum.

Treatment: 50 grams of the formulation of Example 1 administered daily.

Progress: Shrinkage was observed after 4 months.

Verdict: Somewhat effective.

Test example 1-2 6 year old female. Symptoms include a large pediatric tumor mass in the colon disc.

Treatment: 150 grams of the formulation of Example 1 administered daily.

Progress: Almost disappeared after 6 months.

Verdict: Valid.

Test example 1-3 3 year old female. Symptoms include a fist-sized mass around the rectum and in the disk of the colon.

Treatment: 200 grams of the formulation of Example 1 administered daily.

Progress: Completely disappeared after 4 months.

Verdict: Valid.

Test example 1-4 6 year old female. The symptom was a cephalic mass around the rectum.

Treatment: 100 grams of the formulation of Example 2 administered daily.

Progress: After 4 months, it has shrunk to the size of a walnut.

Verdict: Valid.

Test example 1-5 3 year old female. Symptoms include a tumor layer approximately 10 cm near the mesenteric lymph nodes and around the rectum.

Treatment: 200 grams of the formulation of Example 2 administered daily.

Progress: Completely disappeared after 5 months.

Verdict: Valid.

Test example 1-6 4 year old female. Symptoms include two fist-sized masses in the back of the rectum.

Treatment: 300 grams of the formulation of Example 2 administered daily.

Progress: Completely disappeared after 3 months.

Verdict: Valid.

Test example 1-7 4 year old female. Symptoms include several large bulging masses around the rectum.

Treatment: 20 grams of the formulation of Example 4 administered daily.

Progress: The tumor remained walnut-sized and unchanged.

Verdict: Deterioration prevented.

Test example 1-8 5 year old female. The symptom was a cephalic mass around the rectum.

Treatment: 100 grams of the formulation of Example 4 administered daily.

Progress: Almost reduced in size after 5 months.

Verdict: Valid.

Test Example 1-9 3-year-old female. Symptoms include a cephalic mass in the mesentery and around the rectum.

Treatment: 200 grams of the formulation of Example 4 administered daily.

Progress: Completely disappeared after 4 months.

Judgment: Valid test example 1-10 3 year old female. Symptoms include several fist-sized masses in the disc region of the colon.

Treatment: 150 grams of the formulation of Example 5 administered daily.

Progress: Reduced to 4 months.

Verdict: Valid.

Test example 1-11 5 year old female. The symptom was a flat mass approximately 10 cm around the rectum.

Treatment: 300 grams of the formulation of Example 5 administered daily.

Progress: Completely disappeared after 3 months.

Verdict: Valid.

Test example 1-12 6 year old female. Symptoms include a large pediatric tumor mass in the colon disc.

Treatment: Administration of 100 grams of the formulation of Example 6 daily.

Progress: Completely disappeared after 5 months.

Verdict: Valid.

Test example 2 Japanese black cows with an average age of 3 years in an area where fat necrosis is common
The preparation of Example 5 was continuously administered to 300 animals at a rate of 10 grams per day by mixing it with feed.

When these cows were palpated 18 months later, no lumps characteristic of fat necrosis were observed.

That is, a preventive effect was observed in cows aged 3 to 5 years, where fat necrosis occurs frequently.

Claims (1)

[Claims] 1 β-sitosterol, γ-sitosterol,
Bovine fat necrosis characterized in that the active ingredient is a crude product or purified product containing one or more sterols selected from the group consisting of brassicasterol, stigmasterol, campesterol, and γ-sitostanol. Disease prevention and treatment agent. 2. The prophylactic or therapeutic agent according to claim 1, further comprising a physiologically harmless solid or liquid diluent. 3. The prophylactic and therapeutic agent according to claim 1 or 2, further comprising one or more of the following: auxiliaries, additives, bactericides, preservatives, enzymes, antibiotics, and lactic acid bacteria preparations.