JPS59205320A - Preventive and remedy for bovine lipomatosis - Google Patents

Abstract

PURPOSE:To provide a drug containing vegetable sterol as an active component and useful as a remedy and preventive for bovine lipomatosis. CONSTITUTION:The objective drug contains a vegetable sterol (e.g. stigmasterol, ergosterol, etc.) as an active component. The bovine lipomatosis is a lethal disease caused by the swelling of the adipose tissue in the abdominal cavity which presses and disorders the digestive tracts, uterus, kidney, etc. resulting in the sympotoms such as anorexia, constipation, etc. The vegetable sterol was found to have remedying and preventive effects to the disease. The drug is usually used by directly mixing a vegetable sterol or crude vegetable sterol to a feed or by dispersing the sterol in a diluent and adding the dispersion to a feed. The bovine lipomatosis can be cured at a dose of 0.5-60g/day per an adult cattle, and prevented at a dose of 0.05-6g daily. The drug has another advantage of low toxicity.

Description

[Detailed description of the invention] The present invention relates to a preventive and therapeutic agent for fat necrosis in cattle.

Cases of fat necrosis in cattle have been reported both domestically and internationally for a long time, and mainly the fatty tissue in the abdominal cavity becomes a mass, putting pressure on the gastrointestinal tract, uterus, kidneys, etc., resulting in disorders, loss of appetite, and constipation. It is a disease that can lead to death.

It is often found in the serous fat of the kidney, colon, and rectum, and the size and shape of the tumor vary. The reason for this is thought to be due to factors such as the excessive feeding of concentrated feed and lack of roughage in recent cattle breeding.

The current treatment method used by the central government is the administration of coix peas, but it is still far from perfect, and a highly effective drug is desperately needed.

Under these circumstances, the present inventors conducted intensive research on fat necrosis in cattle and found that plant sterols have therapeutic and preventive effects on the disease, leading to the present invention.

The plant sterols that are the active ingredients of the present invention include sterols obtained from various plants, such as stigmasterol, ergosterol, 5,6-cyhydrosterol,
Brassicasterol, campesterol, α-spinasterol, β-spinasterol, γ-spinasterol, δ-spinasterol, β-sitosterol, γ-
sitosterol, fucosterol, zimosterol, anasterol, cerebisterol, evisterol, gamma
- sitostanol, fucosterol, anasterol,
Examples include hyposterol, contrilus tyrol, stigmasterol, and especially β-syl-1-sterol,
Stigmasterol, campesterol, γ-sitosterol, brassicasterol, γ-sitostanol and the like are preferred.

One or more of these may be used in appropriate combination.

Further, these plant sterols may be used in the form of purified products or crude products.

Among plant sterols, for example, stigmasterol comes from soybeans, palms, cottonseeds, kidney beans, green beans, etc., and campesterol comes from rapeseed, wheat, soybeans, rye, etc.
β-sitosterol is widely contained in higher plants such as grains, bark, and leaves in the free state, glycosides, fatty acid esters, etc., and γ-sitosterol can be obtained from peas, carapal beans, rice, soybeans, etc. Crude plant sterols obtained by separating oils from these plants may be used, and crude plant sterols obtained by separating natural vitamin E, which is touted as a health food, from these oils are inexpensive. It also has a great economic advantage because it can be provided at low cost.

The therapeutic agent for preventing and treating bovine fat necrosis of the present invention can be prepared by mixing plant sterols or crude plant sterols with a physiologically harmless solid or liquid diluent or by preparing powders, powders, tablets, capsules, and granules. Although it may be formulated into a suspension, oil, etc., it is usually used by directly mixing it with feed, etc., or by adding it to feed etc. after dispersing it in a diluent.

Other appropriate adjuvants or additives, bactericides, preservatives, enzymes, antibiotics, lactic acid bacteria preparations, etc. can also be used in combination.

The amount of active ingredient used in the therapeutic agent for preventing and treating fat necrosis in cattle of the present invention varies depending on the breed, weight, age, sex, dosing period, plant sterol, etc. of the cattle, but ranges from 0.5 to 60 per adult cattle per day.
Fat necrosis in cattle can be treated by administering 1 to 30 g, preferably 1 to 30 g.

0.05 to 6 g per adult cow per day, preferably 0.1
Fat necrosis in cattle can be prevented by administering 3 to 3 g.

The agent for preventing and treating bovine fat necrosis of the present invention is further characterized by low toxicity.

Furthermore, the therapeutic agent for preventing and treating fat necrosis in cattle of the present invention has a surprising feature in that it can reduce and eliminate fatty liver that has developed and hardened to the size of a fist or a human head.

Next, the present invention will be explained in detail with reference to Examples and Test Examples-3= I will clarify.

Example 1 β-sitosterol 3 parts by weight Corncob meal 65 parts by weight Starch 32 parts by weight were mixed uniformly and prepared.

Example 2 Campesterol 2 parts by weight Stigmasterol 1 part by weight Corncob meal 65 parts by weight Defatted soybean flour 32 parts by weight were mixed uniformly and prepared.

Example 3 20 parts by weight of crude plant sterol (obtained during the production of cottonseed oil), 60 parts by weight of defatted soybean flour, and 20 parts by weight of yeast were uniformly mixed and prepared into pills.

Example 4 Crude plant sterols (obtained during soybean oil production).

4- Approximately 1 as vegetable styrene 5% content) 20 stacks Corn flour 60 parts by weight Talc 20 parts by weight were mixed uniformly and prepared into granules.

Example 5 Crude plant sterol (obtained when vitamin E was separated from the crude plant sterol used in Example 4. Contains approximately 60% as plant sterol) 5 parts by weight Corn cob meal 80 parts by weight Starch 15 parts by weight were uniformly mixed. and prepared.

Example 6 β-sitosterol 2.0 parts by weight Brassicasterol 0.4 parts by weight Campesterol 1.2 parts by weight Stigmasterol 0.4 parts by weight Corncob meal 66 parts by weight Starch 30 parts by weight were mixed uniformly and prepared into granules.

Test Example 1 A test was conducted on 12 Japanese Black female cows that were diagnosed with fat necrosis by palpation during breeding or fattening.

A predetermined amount of the bovine fat necrosis prevention and treatment agent of the present invention was mixed with feed and administered daily, and the progress was observed.

Test example 1-1 A 5 year old female. The symptom was a fist-sized mass around the rectum.

Treatment: 50 grams of the formulation of Example 1 administered daily.

Progress: Shrinkage was observed after 4 months.

Verdict: Somewhat effective.

Test example 1-2 6 male female. Symptoms include a large pediatric tumor mass in the disc region of the colon.

Treatment: 150 grams of the formulation of Example 1 was administered daily.

Progress: Almost disappeared after 6 months.

Verdict: Valid.

Test Example 1-3 3 year old female. Symptoms include a layer of fist-sized masses around the rectum and in the disk of the colon.

Treatment: 200 grams of the formulation of Example 1 administered daily.

Progress: Completely disappeared after 4 months.

Verdict: Valid.

Test example 1-4 6 male female. The symptom was a large mass around the rectum.

Treatment: 100 grams of the formulation of Example 2 administered daily.

Progress: After 4 months, it has shrunk to the size of a walnut.

Verdict: Valid.

Test Example 1-5 3 year old female. The symptom was a layer of approximately loam-sized mass near the mesenteric lymph nodes and around the rectum.

Treatment: 200 grams of the formulation of Example 2 administered daily.

Progress: Completely disappeared after 5 months.

Verdict: Valid.

=7- Test example 1-6 A 4 year old female. Symptoms include two fist-sized masses in the back of the rectum.

Treatment: 300 grams of the formulation of Example 2 administered daily.

Progress: Completely disappeared after 3 months.

Verdict: Valid.

Test example 1-7 A 4 year old female. Symptoms include several large walnut masses around the colon.

Treatment: 20 grams of the formulation of Example 4 administered daily.

Progress: The tumor remained walnut-sized and unchanged.

Verdict: Deterioration prevented.

Test example 1-8 A 5 year old female. Symptoms include six large masses around the colon.

Treatment: 100 grams of the formulation of Example 4 administered daily.

Progress: It has almost shrunk after 5 months.

Verdict: Valid.

Test Example 1-9 3 year old female. Symptoms include a large mass in the mesentery and around the rectum.

=8- Treatment: 200 grams of the formulation of Example 4 administered daily.

Progress: Completely disappeared after 4 months.

Verdict: Valid.

Test Example 1-10 3 year old female. Symptoms include several fist-sized masses in the disc region of the colon.

Treatment: 150 grams of the formulation of Example 5 administered daily.

Progress: Shrinkage after 4 months.

Judgment: Valid.

Test Example 1-11 5 year old female. The symptom was a flat tumor layer around the rectum, approximately 10 cm in diameter.

Treatment: 300 grams of the formulation of Example 5 administered daily.

Progress: Completely disappeared after 3 months.

Verdict: Valid.

Test example 1-12 6 male female. Symptoms include a large pediatric tumor mass in the disc region of the colon.

Treatment: 100 grams of the formulation of Example 6 administered daily.

Progress: Completely disappeared after 5 months.

Verdict: Valid.

Test Example 2 10 grams of the formulation of Example 5 was continued to be administered daily by mixing it with feed to 300 Japanese Black cows, an average of 3 years old, located in an area where fat necrosis frequently occurs.

When these cows were palpated 18 months later, no lumps characteristic of fat necrosis were found.

That is, a preventive effect was observed in cows aged 3 to 5 years, where fat necrosis occurs frequently.

Applicant Kaken Pharmaceutical Co., Ltd. 11- 210-

Claims (1)

[Claims] 1) Preventive and therapeutic agent for cattle fat necrosis containing plant sterols as an active ingredient