JPS6230209A - Dark visual field lighting equipment for microscope - Google Patents
Dark visual field lighting equipment for microscopeInfo
- Publication number
- JPS6230209A JPS6230209A JP16947885A JP16947885A JPS6230209A JP S6230209 A JPS6230209 A JP S6230209A JP 16947885 A JP16947885 A JP 16947885A JP 16947885 A JP16947885 A JP 16947885A JP S6230209 A JPS6230209 A JP S6230209A
- Authority
- JP
- Japan
- Prior art keywords
- objective lens
- ring
- reflected
- light
- ring mirror
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Microscoopes, Condenser (AREA)
Abstract
Description
【発明の詳細な説明】
〈産業上の利用分野〉
本発明は、顕微鏡用の暗視野照明装置の改良に関するも
のである。DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to an improvement in a dark field illumination device for a microscope.
〈従来の技術〉
顕微鏡の観察において、照明光が直接対物レンズに入ら
ないようにし、物体に当たって散乱した光のみによって
物体を観察する方法がある。これが、所謂、暗視野照明
方法と言われるものである。<Prior Art> In microscopic observation, there is a method in which illumination light does not directly enter the objective lens, and the object is observed using only the light that hits the object and is scattered. This is what is called a dark field illumination method.
このような観点に立って、従来から、例えば第３図に示
すように、通常の光源１を対物レンズ２と観察物体を載
せるステージ板３の側方に設置し、照明光を斜めから照
射する方法、或いは第４図に示すように、対物レンズ２
の外周に下向きに照明光を照射する光ファイバを環状に
配列してなるリングライト４を設け、照明光を上方から
照射する方法等が、採用されている。From this point of view, conventionally, as shown in FIG. 3, for example, a normal light source 1 is installed on the side of an objective lens 2 and a stage plate 3 on which an observation object is placed, and illumination light is irradiated obliquely. method, or as shown in FIG.
A method is adopted in which a ring light 4 is provided on the outer periphery of the ring light 4 in which optical fibers that emit illumination light downward are arranged in a ring shape, and the illumination light is emitted from above.
〈発明が解決しようとする問題点〉
しかしながら、上記第３図の照明方法の場合だと、光源
１が１個、精々増やしても３〜４個程度が限度であるた
め、連続した全周方向の照射光が得られない。従って、
観察物体の均一な照明は無理である。<Problems to be Solved by the Invention> However, in the case of the lighting method shown in FIG. irradiation light cannot be obtained. Therefore,
Uniform illumination of the observed object is impossible.
この点で、上記第４図の照明方法では、リングライト４
により全周方向からの照明が可能であるが、ステージ板
３から反射した反射光のうち、対物レンズ２の開口角以
内の光は、当該対物レンズ２に一部直接入射されてしま
う欠点があった。In this regard, in the lighting method shown in FIG. 4 above, the ring light 4
Although it is possible to illuminate from all around the circumference, there is a drawback that some of the light reflected from the stage plate 3 within the aperture angle of the objective lens 2 is directly incident on the objective lens 2. Ta.
本発明は、このような従来の問題点に鑑みてなされたも
ので、照明光を均一に照射すると共に、対物レンズ側へ
の照明光の入射を完全に防止した暗視野照明装置を提供
することを目的とする。The present invention has been made in view of such conventional problems, and an object of the present invention is to provide a dark field illumination device that uniformly irradiates illumination light and completely prevents illumination light from entering the objective lens side. With the goal.
く問題点を解決するための手段〉
この目的を達成する本発明の特徴は、内側に逆円錐形状
の反射面を有する特殊構造のリングミラーと、下向きに
照明光を照射する光ファイバを環状に配列してなるリン
グライトとを用い、このリングミラーを対物レンズと観
察物体の載置されるステージ板の外周近傍に配置し、上
記リングライトをリングミラーの反射面上で上記対物レ
ンズの外周に設けた点にある。Means for Solving the Problems> The present invention is characterized by a ring mirror having a special structure having an inverted cone-shaped reflecting surface on the inside, and an optical fiber that emits illumination light downward in a ring shape. The ring mirror is arranged near the outer periphery of the stage plate on which the objective lens and the observation object are placed, and the ring light is provided on the outer periphery of the objective lens on the reflective surface of the ring mirror. At the point.
〈作用〉
この構成により、リングライトから照射された照明光は
リングミラーで反射され、ステージ板上に照射される。<Operation> With this configuration, the illumination light emitted from the ring light is reflected by the ring mirror and irradiated onto the stage board.
このとき、対物レンズの開口角に対して、リングミラー
の中心径、リングミラーの反射面の傾斜角、リングライ
トの光ファイバの開口角等の各値を適宜選択、設定する
ことにより、照明光が直接対物レンズに入射されること
はない。At this time, the illumination light can be adjusted by appropriately selecting and setting the center diameter of the ring mirror, the inclination angle of the reflective surface of the ring mirror, the aperture angle of the optical fiber of the ring light, etc. with respect to the aperture angle of the objective lens. It is not directly incident on the objective lens.
〈実施例〉
第１図及び第２図は本発明に係ぞ暗視野照明装置の一実
施例を示したものである。<Embodiment> FIGS. 1 and 2 show an embodiment of a dark field illumination device according to the present invention.
図において、１１は顕微鏡の対物レンズ、１２は対物レ
ンズ１１の下方に対向して位置され観察しようとする物
体が載せられるステージ板、１３はその内側に逆円錐形
状の反射面１３ａを有するリングミラー、１４は下向き
に照明光を照射する光ファイバ１５・・・を環状に配列
してなるリングライトである。In the figure, 11 is an objective lens of a microscope, 12 is a stage plate located opposite to the objective lens 11 and on which an object to be observed is placed, and 13 is a ring mirror having an inverted cone-shaped reflective surface 13a inside thereof. , 14 is a ring light formed by annularly arranging optical fibers 15 that emit illumination light downward.
上記リングミラー１３は、対物レンズ１１と同軸として
、当該対物レンズ１１とステージ板１２の外周近傍に配
置すると共に、リングライト１４はリングミラー１３の
反射面１３ａ上で上記対物レンズ１１の外周に当該対物
レンズ１１と同軸的に設けである。The ring mirror 13 is disposed coaxially with the objective lens 11 near the outer periphery of the objective lens 11 and the stage plate 12, and the ring light 14 is placed on the reflective surface 13a of the ring mirror 13 on the outer periphery of the objective lens 11. It is provided coaxially with the lens 11.
しかして、この暗視野照明装置によれば、リングライト
１４の光ファイバ１５・・・下端から下向きに照射され
た照明光は、リングミラー１３の反射面１３ａで反射さ
れ、ステージ板１２上に照射される。このとき、照射光
は、ステージ板１２上の観察物体に当たって散乱し、こ
の散乱光が対物レンズ１１に入射され、観察される。According to this dark field illumination device, the illumination light irradiated downward from the lower end of the optical fiber 15 of the ring light 14 is reflected by the reflective surface 13a of the ring mirror 13 and is irradiated onto the stage plate 12. Ru. At this time, the irradiated light hits the observation object on the stage plate 12 and is scattered, and this scattered light enters the objective lens 11 and is observed.
この際、本発明では、上述したように、対物レンズ１１
の開口角βに対して、リングミラー１３の中心径φＤ、
リングミラー１３の反射面１３ａの傾斜角α、リングラ
イト１４の光ファイバ１５・・・の開口角ψの各値を適
宜選択して最適値に設定しである。従って、リングミラ
ー１３の反射面１３ａで反射された反射光（照明光）は
、ステージ板１２上で再度、反射されても、その再反射
光が対物レンズ１１側に直接入射されることはない。At this time, in the present invention, as described above, the objective lens 11
With respect to the aperture angle β, the center diameter φD of the ring mirror 13,
The inclination angle α of the reflective surface 13a of the ring mirror 13 and the aperture angle ψ of the optical fiber 15 of the ring light 14 are appropriately selected and set to optimal values. Therefore, even if the reflected light (illumination light) reflected by the reflective surface 13a of the ring mirror 13 is reflected again on the stage plate 12, the re-reflected light will not directly enter the objective lens 11 side. .
つまり、完全な暗視野照明が行われる。In other words, complete darkfield illumination is provided.
尚、通常の顕微鏡では、対物レンズの倍率が×４〜×４
０、開口数がＮＡｏ、１〜０．６５、開口角βが５″〜
４０”、対物レンズから観察物体までの距離が１〜ｌＱ
ｍｍ程度と多岐に渡っているが、本発明では、上記のよ
うにリングミラー１３の中心径φＤ、リングミラー１３
の反射面１３ａの１頃斜角α、リングライド１４の光フ
ァイバ１５・・・の開口角ψ等の各値を適宜選択するこ
とにより、あらゆる対物レンズに対応することができる
。In addition, in a normal microscope, the magnification of the objective lens is between ×4 and ×4.
0, numerical aperture is NAo, 1~0.65, aperture angle β is 5″~
40", distance from objective lens to observation object is 1~1Q
The diameter of the ring mirror 13 varies widely, such as approximately mm, but in the present invention, as described above, the center diameter φD of the ring mirror 13,
By appropriately selecting values such as the oblique angle α of the reflecting surface 13a and the aperture angle ψ of the optical fiber 15 of the ring slide 14, it is possible to correspond to any objective lens.
〈発明の効果〉
以上の説明から明らかなように、本発明によれば、特殊
構造のりングミラーと光ファイバを光伝送媒体としたリ
ングライトとの組み合わせにより、比較的安価で、照明
光の均一な照明ができ、且つ対物レンズ側には、観察物
体に当たって散乱した散乱光のみが入射され、照明光が
直接入ることのない完全な暗視野照明が行える優れた顕
微鏡用暗視野照明装置を提供することができる。<Effects of the Invention> As is clear from the above description, according to the present invention, by combining a ring mirror with a special structure and a ring light using an optical fiber as an optical transmission medium, uniform illumination can be achieved at a relatively low cost. It is an object of the present invention to provide an excellent dark-field illumination device for a microscope, which is capable of completely dark-field illumination in which only the scattered light that has hit the observation object is incident on the objective lens side, and no illumination light is directly incident. can.
第１図は本発明に係る顕微鏡用暗視野照明装置の一実施
例を示した部分縦断面図、第２図は上記第１図の装置の
平面図、第３図及び第４図は従来例に係る各顕微鏡用照
明装置の概略を示した説明図である。
図中、１１・・・顕微鏡の対物レンズ、１２・・・ステ
ージ板、
１３・・・リングミラー、
１３ａ・・・リングミラーの反射面、
１４・・・リングライト、
１５・・・光ファイバ、
特許出願人 胚倉電線株式会社第１図
第２図FIG. 1 is a partial vertical sectional view showing an embodiment of a dark field illumination device for a microscope according to the present invention, FIG. 2 is a plan view of the device shown in FIG. 1, and FIGS. 3 and 4 are conventional examples. FIG. 2 is an explanatory diagram schematically showing each microscope illumination device according to the present invention. In the figure, 11... Objective lens of microscope, 12... Stage plate, 13... Ring mirror, 13a... Reflective surface of ring mirror, 14... Ring light, 15... Optical fiber, Patent. Applicant Ukkura Electric Cable Co., Ltd. Figure 1 Figure 2
Claims (1)
周近傍に、当該対物レンズと同軸にして、その内側に逆
円錐形状の反射面を有するリングミラーを配置すると共
に、当該リングミラーの反射面上で前記対物レンズの外
周に、下向きに照明光を照射する光ファイバを環状に配
列してなるリングライトを設け、前記光ファイバからの
照明光をリングミラーで反射させて、前記ステージ板上
に照射することを特徴とする顕微鏡用暗視野照明装置。A ring mirror that is coaxial with the objective lens and has an inverted cone-shaped reflective surface inside the objective lens is disposed near the outer periphery of the objective lens and the stage plate facing the objective lens, and A ring light formed by annularly arranging optical fibers that emit illumination light downward is provided around the outer periphery of the objective lens, and the illumination light from the optical fibers is reflected by a ring mirror and irradiated onto the stage plate. A dark field illumination device for microscopes featuring:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16947885A JPS6230209A (en) | 1985-07-31 | 1985-07-31 | Dark visual field lighting equipment for microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16947885A JPS6230209A (en) | 1985-07-31 | 1985-07-31 | Dark visual field lighting equipment for microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6230209A true JPS6230209A (en) | 1987-02-09 |
Family
ID=15887288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16947885A Pending JPS6230209A (en) | 1985-07-31 | 1985-07-31 | Dark visual field lighting equipment for microscope |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6230209A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5684626A (en) * | 1994-10-25 | 1997-11-04 | Edge Scientific Instrument Company Llc | Center masking illumination system and method |
-
1985
- 1985-07-31 JP JP16947885A patent/JPS6230209A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5684626A (en) * | 1994-10-25 | 1997-11-04 | Edge Scientific Instrument Company Llc | Center masking illumination system and method |
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