JPS62277309A - Controlling agent against miscellaneous algae - Google Patents

Abstract

PURPOSE:A controlling agent against miscellaneous algae, containing (3-amino-3- carboxylpropyl)-methyl phosphinic acid and salt thereof as an active ingredient and capable of controlling noxious miscellaneous algae contaiminating in artificial cultivation without damaging the conchocelis phase of laver. CONSTITUTION:A controlling agent containing (3-amino-3-carboxypropyl)- methylphosphionic acid and a salt thereof, e.g. ammonium, Na or K salt, as an active ingredient. The agent in an amount of 10-500ppm, preferably 50-200ppm expressed in terms of the active ingredient based on seawater is added to a cultivation tank to effectively control noxious green algae, diatom, blue-green algae, etc., growing in cultivation seawater and sticking onto shells, promote growth of conchocelis phase and prevent the contamination of seawater.

Description

Detailed Description of the Invention 3. Detailed Description of the Invention [Field of Industrial Application] The present invention is directed to the cultivation of nori filaments, particularly harmful algae that are present during the artificial cultivation of nori shell filaments. This invention relates to 9Ii algae control rules and methods for controlling algae without causing harm to the body.

[Prior art]

First and foremost, nori is an important food that the Japanese people have eaten since ancient times.
It is a natural food that is indispensable to the Japanese diet, and what we are talking about here is Amanori (hereinafter abbreviated as Nori), which is commonly eaten, and is classified under the nickname of a type of seaweed called primitive red algae. There are various types such as Susabinori and Susabinori. Nori farming technology has made great strides in recent years thanks to the elucidation of the life history of nori, and most of its production is through artificial cultivation. Cultivation work involves filament culture - seedling collection and seedling raising - in accordance with the life history of nori.
This is done during the harvesting process. Filament culture in the seaweed cultivation process is used to create seeds in seaweed cultivation, and the establishment of this technology has made it possible to efficiently harvest seedlings artificially, making mass cultivation of seaweed a reality.

There are two types of filament culture necessary for artificially collecting seaweed seedlings: free filament culture and shell filament culture, and currently shell filament culture is often used. In culturing seashell filaments, nori filaments are grown inside the shells in a seawater layer using shells such as oyster shells, and sporicides released from the mature sporophylls of the shell filaments are used for seedling collection. Since this artificial culture of shell filaments is carried out in seawater, other miscellaneous species may occur on the shells and in the seawater.
Since contaminating algae inhibit the growth of filaments, there is a strong need to establish an appropriate method for controlling contaminating algae. Normally, filamentous culture is carried out in early spring with fruit spores attached and carried out from spring to autumn, but especially during the filamentous growth period from spring to summer when the water temperature rises and the intensity of sunlight increases, green algae, blue-green algae, diatoms, etc. Contamination occurs, let it go! As a result, filament growth is significantly inhibited. Methods for controlling these algae include washing the shells, killing seawater, and changing the water, but these methods are not effective and are costly, so there is a strong desire to establish effective control methods. .

[Point J while the invention is trying to solve]

In the process of culturing seaweed shell filaments, the present invention involves adding a small amount to the culture seawater during the period from spring to summer when the filaments have grown considerably and when the growth of algae is significant due to rising temperatures and increased illuminance. It is an object of the present invention to provide a 9I algae control agent and a method for controlling algae that effectively control algae and have no effect on the growth, sporangium formation, and maturation of shell filaments.

[Means and effects for solving the problem] The present inventors searched for a drug that would control contaminating miscellaneous algae without harming the filaments of nori (3-amino-3-
It has been found that (carboxypropyl)-methylphosphinic acid and its salts can extremely effectively control algae without harming filaments. (3-Amino-3-carboxypropyl)-methylphosphinic acid and its salts (e.g. ammonium salt, sodium salt, potassium salt, etc.) are derivatives of the natural substance glutamic acid and can be produced by methods known in the literature (German Patent No. 116236 specification,
J, Org, Chem, 29, 832 (1964),
Japanese Patent Publication No. 48-91019 and Japanese Patent Publication No. 57-26564
Publication No.). Also, in Japanese Patent Publication No. 57-26564 (3
-Amino-3-carboxypropyl)-methylphosphinic acid derivatives have been described as being used as herbicides, but the characteristic of this family of compounds is that they are so-called non-selective weed killers that kill all the plants in the stratum. It belongs to the drug,
There has been no study at all about its action on lower plants such as ii, much less on algae that occur in seawater, and its selectivity towards nori filaments is completely unknown. As a result of various studies on methods for controlling various algae contaminating the culture of nori filaments, the present inventors found that the composition according to the present invention is effective against the filaments in culture of nori filaments using shells in seawater. The present inventors have discovered that the present invention can be selectively and safely used to effectively control harmful green algae, diatoms, blue-green algae, etc. that adhere to cultured seawater and shells.

That is, the present invention provides an algae control agent for culturing nori shell filaments, which is characterized by containing (3-amino-3-carboxypropyl)-methylphosphinic acid and its salt as an active ingredient, and the addition of the same to a culture tank. This is a method for controlling algae that is characterized by:

When operating according to the method of the invention, (3-amino-3-
Carboxypropyl)-methylphosphinic acid and its salts are added to the seawater to be treated at a predetermined concentration, and are used as active ingredients in an amount of 10 to 500 ppm, preferably 50 to 20 ppm.
Use at 0 ppm. When added to a culture tank when replacing seawater, it can suppress the growth of algae for 3 to 5 weeks, promote the growth of filamentous bodies, and prevent seawater contamination. Usually, it is effective to use it once to three times between 3 and 5a during the period when the occurrence of algae is significant.

The (3-amino-3-carboxypropyl)-methylphosphinic acid and its salts used in the present invention are prepared by adding a surfactant and the like to a workpiece, etc., and are thereby used to deliver the active ingredient to algae. It increases the affinity and stabilizes the anti-algae effect, and usually an appropriate amount, that is, 10% of the amount of active ingredient.
~70%, preferably 20 to 50% of additives such as surfactants are added to the algae control agent of the present invention, and one or more other algae control actives and seawater fungicides are added. It is also possible to prepare mixed preparations containing vitamins, minerals, etc., and synergistic effects can be expected in some cases.

〔Example〕

Next, the algae controlling agent and the controlling method according to the present invention will be explained with reference to Examples, but the present invention is not limited thereto.

Formulation Example 1 20g of ammonium (3-amino-
3-carboquinpropyl)-methylphosphinate)
The mixture was dissolved, and 4 parts of 7Nhytol 20BS (manufactured by Kao ■, a formulation containing lauryl betaine as the main ingredient) was added and stirred to homogenize to obtain 100 parts of a liquid preparation containing 20% of the active ingredient.

Formulation Example 2 20 parts of ammonium-(3-amino-3
-carboxypropyl)-methylphosphinate? 8
Then, add 9 parts of Lebenol (manufactured by Kao ■, a preparation with the main ingredient polyoxine ethylene alkyl ether sodium sulfate) and stir to homogenize.
I got the department.

Test example 1. (Efficacy test) (Reinforced seawater SWM-II [How to prepare modification liquid) Table A
SWM-III prescription drug name Stock solution 1 concentration Mixed amount Sodium nitrate (NaNOt) 1.0M (85g/l)
2ml Sodium phosphate (Naz) IPOn) 50m
M (7,8g/i!) 2m1 EDTA (NaJ
DTA) 15mM (5,6g/n) 2
ml ferric chloride (FeC13) 1.0mM (27
0mg/f) 2m1Table B PI-Metal prescription drug name Quantity Mixed amountFilter the seawater with absorbent cotton, add the mixed amounts shown in Tables A and B to 1i of seawater, add 500mg of Trisbach, and add H(l Adjust the pH to 7 and sterilize.

The reinforced seawater SWM-former modified solution prepared above was placed as a culture medium in a 100 ml Erlenmeyer flask, and the test agent was added to a predetermined concentration. Next is Hordemium (a type of green algae)
was added and cultured stationary at 23° C., illuminance 2000 lfx, light period 12 hours/day, and the viability and color of the hordemium was observed with the naked eye and with a microscope over time to evaluate the effect. The results are shown in Table-1.

Test example 2. (Efficacy test) Reinforced water SWM-old modified solution was used as a culture medium.
Place in an Erlenmeyer flask and add the test drug to the specified concentration. Next, add Navicula (a type of diatom) to 23°
The cells were cultured stationary under a C1 illuminance of 2000 lux and a light period of 12 hours/day, and the viability and color of Navicula were observed with the naked eye and with a microscope over time to evaluate the effect. The results are shown in Table-2.

Test example 3. (Efficacy and phytotoxicity test) Low temperature sterilized (70°C 130 minutes) natural nun per water 700m
1 as a bat (width 20.5 cm, 1215 cm, height 5
cm), and after adding nutrients, the test drug was added to a predetermined concentration. Next, the fruit spores of Narawasu Sabinori will develop in two months, and four oyster shells with green algae, diatoms, and blue-green algae attached to their surfaces are arranged at the bottom of a vat and heated at 23°C and with an illuminance of 2.
000 Rufus, statically cultured at a light period of 1258 hours/day,
The effect on the oyster rise of the specimen and in seawater and the influence on the filament were observed with the naked eye over time. The results are shown in Table-3 and Table-4.

・Table-1 Control activity +; Dead ±: Mostly dead, but normal things are mixed in. -2 Normal Table-2 Control activity +: Dead ±: Mostly dead, but normal things are mixed. m:
Normal Table-3 Control effect +: Dead ±: Most withered and some are alive 12 Invalid Table-4 Phytotoxicity test +: Phytotoxicity M: No phytotoxicity [Effect of the invention] From the test results, it is found that the present invention is effective. The algae control agent and method are excellent choices because they can effectively control the pheasant (green algae, diatoms, blue-green algae, etc.) that contaminates during the artificial cultivation of the nori shell filaments without causing any harm to the nori shell filaments. It is clear that it has a certain gender.

Claims (2)

[Claims] (1) An algae control agent for culturing seaweed shell filaments, which contains (3-amino-3-carboxypropyl)-methylphosphinic acid and its salt as an active ingredient. (2) A method for controlling algae for cultivating nori shell filaments, which comprises adding (3-amino-3-carboxypropyl)-methylphosphinic acid and its salt to a culture tank during culture of nori shell filaments.