JPS62253372A - Production of algae and device therefor - Google Patents
Production of algae and device thereforInfo
- Publication number
- JPS62253372A JPS62253372A JP9668786A JP9668786A JPS62253372A JP S62253372 A JPS62253372 A JP S62253372A JP 9668786 A JP9668786 A JP 9668786A JP 9668786 A JP9668786 A JP 9668786A JP S62253372 A JPS62253372 A JP S62253372A
- Authority
- JP
- Japan
- Prior art keywords
- light
- tank
- algae
- zone under
- conditions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title claims description 5
- 230000014759 maintenance of location Effects 0.000 claims abstract description 27
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 8
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 8
- 229940082787 spirulina Drugs 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 241000195649 Chlorella <Chlorellales> Species 0.000 abstract description 3
- 230000002093 peripheral effect Effects 0.000 abstract 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000005273 aeration Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004925 Acrylic resin Substances 0.000 description 1
- 229920000178 Acrylic resin Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
[産業上の利用分野]
本発明は藻類の製造方法および装置に関し、詳しくは蛋
白源として有用なりロレラ、スピルリナ等の藻類を効率
よく製造する方法ならびにそのために使用する小型化さ
れた装置に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method and apparatus for producing algae, and more specifically, a method for efficiently producing algae such as Lorella and Spirulina, which are useful as a protein source, and a compact device used for the purpose. related to standardized equipment.
[従来の技術とその問題点]
藻類の工業的培養方法として、従来より受光面積を大き
くとるために広い面積を有する水深の浅い池等を利用し
屋外で行なう方法や特開昭55−1132980号に示
されている如く、屋内で人工的に設定した条件下で行な
う方法等が知られている。[Prior art and its problems] As an industrial method for cultivating algae, there is a conventional method of cultivating algae outdoors using a shallow pond or the like with a wide area in order to increase the light-receiving area, and Japanese Patent Laid-Open No. 55-1132980. As shown in Figure 2, there are known methods for performing this indoors under artificially set conditions.
しかし、前者は広大な面積を必要とすることから実施可
能な場所が制限され、後者は人工的条件の設定に多大の
設備と費用を要する上に管理が困難である等の欠点があ
る。However, the former method requires a vast area, which limits the places where it can be implemented, and the latter method requires a large amount of equipment and expense to set artificial conditions, and it is difficult to manage.
[問題点を解決するための手段]
上記のような問題点を解決すべく検討を重ねた結果、藻
類の培養を明条件の帯域と暗条件の帯域に分けて行ない
、両帯域を連通して培養液を循環させ、かつ両帯域の容
量比を特定することによって目的が達成されることを見
出し、かかる知見に基いて本発明を完成した。本発明者
は、藻体濃度が0.5g/i’ (00で1.0)以上
になると、水深に関係なく大半の光は液中で減衰し、内
部にまで到達せず、光合成反応は大部分が受光面で行な
われることを確認し、上記の如く2つの帯域に分けて培
養した方が効率的であることを知見したのである。[Means for solving the problem] As a result of repeated studies to solve the above-mentioned problems, we decided to culture algae separately in a light condition zone and a dark condition zone, and to connect both zones. It was discovered that the objective could be achieved by circulating the culture solution and specifying the volume ratio of both zones, and based on this knowledge, the present invention was completed. The present inventor found that when the algae concentration exceeds 0.5 g/i' (1.0 in 00), most of the light attenuates in the liquid regardless of the water depth, does not reach the interior, and the photosynthetic reaction does not occur. They confirmed that most of the activity was carried out on the light-receiving surface, and found that it was more efficient to culture in two zones as described above.
すなわち本発明は第1に、藻類を製造するにあたり、明
条件の培養帯域と暗条件の培養帯域とに分け、両帯域を
連通して循環させると共に、明条件の培養帯域と暗条件
の培養帯域の容量を前者:後者= 1 : 0.1〜1
:5として培養することを特徴とする藻類の製造方法で
ある。That is, the present invention firstly aims to produce algae by dividing the algae into a cultivation zone under light conditions and a cultivation zone under dark conditions, and circulating the two zones in communication with each other, as well as creating a cultivation zone under light conditions and a cultivation zone under dark conditions. The capacity of the former: latter = 1: 0.1~1
This is a method for producing algae, characterized by culturing it as: 5.
本発明の第2は、明条件の培養帯域を形成する受光槽と
暗条件の培養帯域を形成する滞留槽よりなり、光透過性
の素材を主材とする受光槽の容量をlとしたとき滞留槽
の容量を0.1〜5とし、かつ滞留槽下部と受光槽なら
びに滞留槽上部と受光槽をそれぞれ接続する移送管を設
けたことを特徴とする藻類の培養装置である。The second aspect of the present invention consists of a light-receiving tank forming a culture zone under light conditions and a retention tank forming a culture zone under dark conditions, where the capacity of the light-receiving tank mainly made of a light-transmitting material is 1. This algae culturing apparatus is characterized in that the capacity of the retention tank is 0.1 to 5, and that transfer pipes are provided to connect the lower part of the retention tank and the light receiving tank, and the upper part of the retention tank and the light receiving tank, respectively.
本発明に用いられる藻類には特に制限はなく、クロレラ
、スピルリナなどの各種藻類を任意に使用することがで
きる。The algae used in the present invention is not particularly limited, and various algae such as chlorella and spirulina can be used arbitrarily.
明条件の培養帯域は太陽光2人工光(キセノンランプな
ど)等を受光して藻類の増殖が盛んに行なわれる帯域で
あり、暗条件の培養帯域は植種的な受光をしないで培養
を行なう帯域である。The cultivation zone under bright conditions is a zone where algae actively proliferate by receiving sunlight 2 artificial light (such as a xenon lamp), etc., and the cultivation zone under dark conditions is a zone where cultivation is performed without receiving light for seeding purposes. Bandwidth.
明条件の培養帯域と暗条件の培養帯域のそれぞれの培養
液量を調節して培養することが必要であり、容量比で前
者:後者= 1 : 0.1〜1:5、好ましくはl:
1〜1:2とする。容量比が前者1に対して後者5以下
であると、第1図に示した如く、藻類の増殖が良好であ
り、後者が2程度以下では明条件の培養帯域のみで培養
した場合とほぼ同程度の増殖が認められる。しかし、明
条件の培養帯域のみとすることは、前を乙従来技術につ
いて指摘したような欠点が生じ、経済的等の理由がらも
望ましくない。It is necessary to adjust the volume of culture solution in the culture zone under light conditions and the culture zone under dark conditions, and the volume ratio of the former to the latter = 1:0.1 to 1:5, preferably l:
The ratio is 1 to 1:2. When the capacity ratio of the former is 1 and the latter is 5 or less, the growth of algae is good, as shown in Figure 1, and when the latter is about 2 or less, the growth is almost the same as when culturing only in the culture zone under light conditions. Some degree of proliferation is observed. However, using only the cultivation zone under light conditions causes the drawbacks mentioned above regarding the prior art, and is not desirable for economical and other reasons.
上記両帯域を連通し、培養液を循環させながら藻類を培
養することにより、藻類の増殖を効率的に行なうことが
できる。By communicating the above two zones and culturing algae while circulating the culture solution, algae can be grown efficiently.
藻類の培養は二酸化炭素と光エネルギーを利用して行な
う方法のほか、グルコース等の糖類や酢酸等の有機酸類
などを主炭素源として行なう方法もあり、本発明では使
用する藻類の性質などを考慮して適切な方法を採用して
行なえばよい。その場合に使用する基本培地についても
既知のものの中から適宜選択すればよい。In addition to culturing algae using carbon dioxide and light energy, there are also methods that use sugars such as glucose and organic acids such as acetic acid as the main carbon source.In the present invention, the characteristics of the algae used are taken into consideration. This can be done by adopting an appropriate method. In that case, the basic medium to be used may be appropriately selected from known ones.
その他の培養条件、たとえばpH,温度9時間。Other culture conditions, such as pH and temperature for 9 hours.
攪拌方法、光照射方法等に関しても既知の条件の範囲内
で設定すればよく、本発明を実施するための特別な条件
を設ける必要はない。The stirring method, light irradiation method, etc. may be set within the range of known conditions, and there is no need to set special conditions for carrying out the present invention.
次に、本発明の方法を実施する際に用いられる装置につ
いて説明する。第2図および第3図は該装置の実施例で
ある。Next, the apparatus used when carrying out the method of the present invention will be explained. FIGS. 2 and 3 are embodiments of the device.
受光槽1は明条件の培養帯域を形成するものであり、槽
の形状としては円柱状、角柱状などがあり、その容量は
使用目的、設置場所等を考慮して適宜決定すればよいが
、実用上10〜1,000ρ程度が好適である。受光槽
は複数個設けてもよい。また、受光槽は光透過性の素材
を主材としたものであり、底面を除く全周面が透明で受
光し易いものであることが好ましい、受光槽は太陽光も
しくは人工光を効率よく受光しうるように設置すべきで
あり、設置場所により水平、垂直、傾斜等の適切な方向
に向けて設置すればよい、なお、受光槽の培養液を移送
管を経て後記する滞留槽に循環させる場合、藻類に損傷
を与えないために、エアーリフト法や水車法を採用する
ことが望ましく、そのために必要な器具を設置する。第
2図はエアーリフト用の配管4を設けており、第3図で
は滞留槽2と受光槽1の間に曝気層5を設けている。The light-receiving tank 1 forms a cultivation zone under bright conditions, and the shape of the tank can be cylindrical, prismatic, etc., and its capacity may be determined as appropriate in consideration of the purpose of use, installation location, etc. Practically speaking, about 10 to 1,000 ρ is suitable. A plurality of light receiving tanks may be provided. In addition, the light receiving tank is mainly made of a light-transmitting material, and it is preferable that the entire circumference except the bottom is transparent and easy to receive light.The light receiving tank should be able to efficiently receive sunlight or artificial light. Depending on the installation location, it may be installed in an appropriate direction, such as horizontally, vertically, or tilted. Furthermore, the culture solution in the light receiving tank should be circulated through the transfer pipe to the retention tank described later. In such cases, it is preferable to use the air lift method or water wheel method in order to avoid damaging the algae, and the necessary equipment should be installed for this purpose. In FIG. 2, piping 4 for air lift is provided, and in FIG. 3, an aeration layer 5 is provided between the retention tank 2 and the light receiving tank 1.
滞留槽2は暗条件の培養帯域を形成するものであり、槽
の形状は特に制限はなく、受光槽と同様に円柱状、角柱
状などがある。滞留槽2は前記受光槽の容量の0.1〜
5倍の容量を有していることが必要である。この範囲外
、とりわけ上限を超えると、藻類の増殖が著しく低下す
るので好ましく゛ ない、滞留槽の材質については受光
槽の場合のような制約はなく、任意に選定しうる。なお
、槽の・蓋部分を透明にすれば受光できるので、藻類の
増殖に好影響を与える。滞留槽には製品抜出口2通気管
、二酸化炭素供給管、熱交換用配管、 pH計。The retention tank 2 forms a culture zone under dark conditions, and the shape of the tank is not particularly limited, and may be cylindrical, prismatic, etc. like the light-receiving tank. The retention tank 2 has a capacity of 0.1 to 0.1 of the capacity of the light receiving tank.
It is necessary to have five times the capacity. If it is outside this range, especially if it exceeds the upper limit, the growth of algae will be significantly reduced, which is undesirable.The material of the retention tank is not subject to any restrictions as in the case of the light receiving tank, and can be selected arbitrarily. Additionally, if the lid of the tank is made transparent, it will be able to receive light, which will have a positive effect on the growth of algae. The retention tank is equipped with two product outlet ports, a ventilation pipe, a carbon dioxide supply pipe, a heat exchange pipe, and a pH meter.
温度計などの常用の配管9機器類を適宜取り付−けるこ
とができる。Nine commonly used piping devices such as thermometers can be installed as appropriate.
受光槽1と滞留槽2は、滞留槽下部と受光槽ならびに滞
留槽上部と受光槽をそれぞれ移送管3により接続されて
いる。所望により、第3図に示した如く滞留槽下部と受
光槽を結ぶ配管の中間部に曝気槽5を設けることにより
エアーリフト効果も生じ、受光槽の形状、設置方法(水
平、垂直、傾斜)などを任意に選定することが可能とな
る。The light-receiving tank 1 and the retention tank 2 are connected by a transfer pipe 3, which connects the lower part of the retention tank to the light-receiving tank and the upper part of the retention tank to the light-receiving tank, respectively. If desired, an air lift effect can be created by providing an aeration tank 5 in the middle of the pipe connecting the lower part of the retention tank and the light receiving tank as shown in Fig. 3, and the shape and installation method of the light receiving tank (horizontal, vertical, inclined) etc., can be selected arbitrarily.
[発明の効果]
本発明によれば、クロレラ、スピルリナ等の藻類を効率
よく製造することができ、たとえばスピルリナの培養の
場合、年間通じての平均収率は0.3〜0.6 g/i
)・日程度が期待できる。[Effects of the Invention] According to the present invention, algae such as chlorella and spirulina can be efficiently produced. For example, in the case of culturing spirulina, the annual average yield is 0.3 to 0.6 g/ i
)・You can expect about a day.
装置の面からは、受光槽と滞留槽に分割したことにより
小型化を図ることができ、さらに受光槽を複数個設置し
ておきそれぞれの稼動を調節することによって藻類の生
産量を制御することが可能である。In terms of equipment, it can be made smaller by dividing it into a light-receiving tank and a retention tank, and it is also possible to control the amount of algae production by installing multiple light-receiving tanks and adjusting the operation of each one. is possible.
[実施例] 次に、本発明の実施例を示す。[Example] Next, examples of the present invention will be shown.
実施例
下記仕様により第2図に示した系でスピルリナ・プラテ
ンシス(Spirulina platensis、ア
フリカのチャド湖より分gl)を培養した。EXAMPLE Spirulina platensis (1 ml from Lake Chad in Africa) was cultured in the system shown in FIG. 2 according to the following specifications.
受光槽: 0.1+*φX1.O+++H円柱状。Light receiving tank: 0.1+*φX1. O+++H cylindrical.
材質 透明アクリル樹脂 滞留槽: 0.35層φ×0.81H円柱状。Material: Transparent acrylic resin Retention tank: 0.35 layer φ x 0.81H cylindrical shape.
材質 不透明塩化ビニル樹脂 培養条件: pH9,5,温度30℃。Material: Opaque vinyl chloride resin Culture conditions: pH 9.5, temperature 30°C.
太陽光照射lO時間/日。Solar irradiation lO hours/day.
通気量(受光槽151)/分、滞留槽67分)循環液量
250〜30011時
受光槽と滞留槽内の培養液量(容量比)を変化させて7
日間培養を続け、スピルリナの収率を比較した。結果を
第1図に示す。Aeration rate (light receiving tank 151)/min, retention tank 67 minutes) Circulating fluid volume 250 to 300 11 hours By changing the amount of culture solution (capacity ratio) in the light receiving tank and retention tank 7
Cultivation was continued for several days, and the yield of Spirulina was compared. The results are shown in Figure 1.
第1図は受光槽と滞留槽の培養液量(容量比)を変化さ
せたときの藻類の収率を示すグラフである。第2図およ
び第3図は本発明を実施する装置の態様を示す見取図で
ある。
1・・・受光槽 2・・・滞留槽3・・・移送
管 4・・・通気管5・・・曝気槽
6・・・通気管7・・・製品抜出口 8・・・熱交
換用配管特許出願人 沖縄石油精製株式会社
同 沖 縄 県収牢0/lym
−a)
双生α/lB)FIG. 1 is a graph showing the yield of algae when the amount (volume ratio) of the culture solution in the light-receiving tank and the retention tank is changed. FIGS. 2 and 3 are sketches showing aspects of an apparatus for carrying out the present invention. 1... Light receiving tank 2... Retention tank 3... Transfer pipe 4... Ventilation pipe 5... Aeration tank
6...Vent pipe 7...Product outlet 8...Heat exchange piping Patent applicant Okinawa Oil Refining Co., Ltd. Okinawa Prefectural Prison 0/lym
-a) Twin α/lB)
Claims (4)
暗条件の培養帯域とに分け、両帯域を連通して循環させ
ると共に、明条件の培養帯域と暗条件の培養帯域の容量
を前者:後者=1:0.1〜1:5として培養すること
を特徴とする藻類の製造方法。(1) When producing algae, the cultivation zone is divided into a cultivation zone under light conditions and a cultivation zone under dark conditions, and both zones are connected and circulated, and the capacity of the cultivation zone under light conditions and the cultivation zone under dark conditions is reduced to the former. A method for producing algae, characterized in that the latter is cultured at a ratio of 1:0.1 to 1:5.
記載の藻類の培養方法。(2) The method for culturing algae according to claim 1, wherein the algae is Spirulina.
培養帯域を形成する滞留槽よりなり、光透過性の素材を
主材とする受光槽の容量を1としたとき滞留槽の容量を
0.1〜5とし、かつ滞留槽下部と受光槽ならびに滞留
槽上部と受光槽をそれぞれ接続する移送管を設けたこと
を特徴とする藻類の培養装置。(3) It consists of a light-receiving tank that forms a culture zone under light conditions and a retention tank that forms a culture zone under dark conditions, and when the capacity of the light-receiving tank whose main material is a light-transmitting material is 1, the retention tank is 1. An algae culturing device having a capacity of 0.1 to 5, and comprising transfer pipes connecting the lower part of the retention tank and the light receiving tank, and the upper part of the retention tank and the light receiving tank, respectively.
を介在させた特許請求の範囲第3項記載の装置。(4) The device according to claim 3, wherein one gas layer is interposed in the transfer pipe connecting the lower part of the retention tank and the light receiving tank.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9668786A JPS62253372A (en) | 1986-04-28 | 1986-04-28 | Production of algae and device therefor |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9668786A JPS62253372A (en) | 1986-04-28 | 1986-04-28 | Production of algae and device therefor |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS62253372A true JPS62253372A (en) | 1987-11-05 |
Family
ID=14171703
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9668786A Pending JPS62253372A (en) | 1986-04-28 | 1986-04-28 | Production of algae and device therefor |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS62253372A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014515931A (en) * | 2011-05-25 | 2014-07-07 | コリア インスティチュート オブ オーシャン サイエンス アンド テクノロジー | Microalgae biofuel production plant, biofuel production culture tank, and microalgae biofuel production method |
-
1986
- 1986-04-28 JP JP9668786A patent/JPS62253372A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014515931A (en) * | 2011-05-25 | 2014-07-07 | コリア インスティチュート オブ オーシャン サイエンス アンド テクノロジー | Microalgae biofuel production plant, biofuel production culture tank, and microalgae biofuel production method |
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