JPS62246591A - Production of extraction of active ingredient in gymnema-sylvestre r-br - Google Patents

Abstract

PURPOSE:To extract only an active ingredient and obtain a stable sterilized extract, by extracting dry leaves of Gymnema Sylvestre R.Br with a water- containing ethanol at a specific temperature, followed by solid-liquid separation and an extraction liquid is concentrated under reduced pressure to make a solute concentration higher than a specific level. CONSTITUTION:Dry leaves of Gymnema Sylvester R.Br are brought into contact with a water-containing ethanol of >=60%, preferably 60-80% concentration at 30-70 deg.C to extract an eluting substance. Then, after solid-liquid separation, the obtained extraction liquid is concentrated under reduced pressure at 40-70 deg.C to make the solute concentration higher than 10% and obtain the aimed extract.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing an extract of active ingredients from dried leaves of Gymnema sylvestre.

Conventional technology Gymnesa, 5ylve.
stre R.

Br, - hereafter referred to as GS) is a plant belonging to the family Asclepiadaceae that grows naturally in tropical and subtropical countries such as India, China, Southeast Asia, and Africa, and its hot water extract is known to have a sweet taste suppressing effect. It has also been found to have the effect of inhibiting sugar absorption in the intestinal tract, and can be used for medicinal purposes and low-calorie foods and drinks.

The active ingredient in GS that exhibits the sweet taste suppressing effect and the sugar absorption suppressing effect in the intestinal tract is called gymnemic acid.

Gymnemic acid has homologues A1-A4, and its basic structure is that of gymnemagenin (3β, 16β,
It is a glycoside consisting of a derivative of (21β, 22α, 23.28-hexahydroxyocyan-12ene) and D-glucuronic acid. Furthermore, the hydroxyl group of triterpenes is esterified with various organic acids, and the types of organic acids differ for each homolog.

In India, where GS is traditionally used, the dried leaves themselves are eaten and used as a herbal medicine to treat diabetes and other diseases. However, it has been argued that the medicinal component lies in the GSS water-oil extract, and it is thought that this is probably due to the interaction of the complex components including the above-mentioned gymnemic acid.

GS dried leaves are collected from their natural habitat in India and other countries, and after drying in the sun, they are packaged in jute bags and distributed. Quality is inconsistent due to defects.

Furthermore, because they are not normally kept under sanitary control, they are often contaminated with microorganisms, especially soil bacteria and spore bacteria. Therefore, there are many problems not only in using the dried leaves themselves but also in processing them.

Furthermore, due to the dry nest of plants, when extracting the active ingredients, there is a risk that the extraction conditions such as temperature may affect the active ingredients.
Since the S aqueous extract has extremely strong foaming properties and is difficult to concentrate, a method for extracting the active ingredients of GS and formulating it into a formulation has not yet been established.

8. The present invention efficiently extracts the active ingredient from dried GS leaves without affecting the active ingredient, and also enables easy formulation by pulverizing it or blending it with other ingredients. The object of the present invention is to provide a method for producing a highly concentrated sterile extract.

Structure of the Invention The method for producing the active ingredient extract of Gymnema sylvestre according to the present invention involves contacting dried leaves of Gymnema sylvestre with aqueous ethanol with a concentration of 60% or more at a temperature of 30°C to 70°C to extract the eluted substances, and extracting the eluted substances. Separate the liquid and add the resulting extract to 40~
It is characterized in that it is concentrated under reduced pressure at 70°C to a solute concentration of 10% or more.

GS is a climbing plant that grows entwined with Takagi Takagi, and GS! i! ! When collecting ffi and some of the branches, GS dried leaves refer to dried GS nests mixed with a small amount of petioles and branches.

By using aqueous ethanol with a concentration of 60% or more when extracting GS, it is possible to simultaneously improve the extraction rate of GS active ingredients, sterilize the extract, and suppress foaming when concentrating the extract.

That is, by contact-treating GS dried leaves at 30°C to 701°C using 60% or more ethanol, preferably 60 to 80% aqueous ethanol, no microorganisms are detected in the extract, and the extraction efficiency is improved compared to hot water extraction. Also, foaming can be suppressed during concentration. When the extracted components were confirmed by TLC, spots similar to those of the hot water extract were confirmed.

Note that during extraction with water-containing ethanol, the extraction efficiency can be further improved by shredding or crushing the dried GS leaves.

The water-containing ethanol extract is separated into solid and liquid from dried confectionery residue and impurities by precision filtration using a paper filter or the like. Since the obtained extract is derived from plants and may change due to heat, it is concentrated under reduced pressure at 40 to 70°C.

When concentrating the extract, the GS hot water extract has extremely strong foaming properties, making it difficult to concentrate the extract. In the water-containing ethanol extract, there is little foaming during the evaporation process of the ethanol portion, and the foaming only becomes strong after the ethanol has evaporated, at a stage when the water portion becomes the main evaporator. Therefore, the difficulty caused by foaming in the concentration process of a hydrous ethanol extract is significantly reduced compared to that of an aqueous extract.

That is, when extracting the active ingredient, by appropriately selecting the concentration and amount ratio of aqueous ethanol to GS dried confectionery, the solute concentration can be reduced to 1 by only evaporating the ethanol portion in the concentration step.
It can be set to 0% or more.

Solution 1 after concentration! ! The higher the concentration, the more convenient it is to blend with other ingredients, and the easier drying will be. However, if you try to make the concentration too high, the ethanol portion will evaporate and the foaming properties will become stronger as mentioned above, so It is appropriate that the solute concentration is about 10 to 20%.

The extract concentrated to 10% or more can be dried into a powder by spray drying or freeze drying. When spray drying, the drying atmosphere should be maintained at a temperature of 70% to prevent the active ingredients from deteriorating.
Care must be taken to ensure that the temperature does not exceed ℃.

By using aqueous ethanol with a concentration of 60% or more as an extractant, it is possible to simultaneously improve the extraction rate of GS active ingredients, sterilize the extract, and suppress foaming during extract concentration. By setting the concentration temperature to 70° C. or lower, deterioration of the GS active ingredient can be prevented.

Example: 51 kg of dried GS was cut with a homogenizer ~99%
After adding 8 g of ethanol and 4 g of water and keeping them in contact for 16 hours with stirring at 30°C, the extract and the residue were separated using a paper filter, and the remaining liquid was again mixed with 99% ethanol 8 g.
After adding 4 liters of water and keeping in contact for 16 hours with stirring at 30°C, the extract and residue were separated again using a paper filter. Both extracts were combined and concentrated under reduced pressure at 40 to 60°C to obtain a solute concentration of 15. %. When the extracted components were confirmed by TLC, spots similar to those of the water extract were confirmed.

When this concentrate was spray-dried, a powder with a water content of 5% was obtained.
I got g.

Comparative Example 1 kg of GS dried leaves was cut into pieces using a homogenizer, 12 liters of water was added, and the mixture was left in contact with the mixture for 16 hours while stirring at 50°C. The extract and the residue were separated using a paper filter, and 12 Jl of water was added to the residue again. After contacting for 16 hours with stirring at 50°C, the extract and residual liquid were separated again using a paper filter. When the two extracts were combined and concentrated under reduced pressure at 40 to 60°C, foaming gradually became more intense. It was impossible to concentrate. Emi Tatsuno] Only the active ingredients are extracted from Gymnema sylvestre dried confectionery, which is unstable in quality and often contaminated with microorganisms, resulting in a sterile extract with stable quality. can be obtained.

Claims (1)

[Claims] Dried leaves of Gymnema sylvesta are brought into contact with aqueous ethanol with a concentration of 60% or more at a temperature of 30°C to 70°C to extract eluted substances, followed by solid-liquid separation, and the resulting extract is concentrated under reduced pressure at 40 to 70°C. A method for producing an extract of active ingredients of Gymnema sylvestre, characterized in that the solute concentration is 10% or more.