JPS62240837A - Automatic cell sorter - Google Patents

Abstract

PURPOSE:To obtain a sorter which enables the alternation of the type of cells to be reviewed without redoing automatic sorting of the cells, by memorizing the positions of all the cells on spacemen to be covered by one automatic sorting during the execution thereof. CONSTITUTION:This sorter is provided with an image processor which automatically sort cells by photoelectrically converting a microscope image of the cells smeared on slide specimens, a slide storage mechanism for storing specimens, a slide carrier which automatically supplies a slide specimen to a cell inspecting position from the storage mechanism and storing it into the storage mechanism after the execution of one automatic sorting and a controller for controlling the operation of the image processor and the storage mechanism. The sorter memorizes serial inspection number of slide specimens, the results of automatic sorting of all cells sorted and the positions of the cells of the slide specimen during the sorting operation. Then, after the end of automatic sorting of specimens, the slide specimens stored are supplied again to a cell inspecting position of a microscope sequentially with the slide carrier whenever the type of any cell is specified thereby enabling the observation of the specified cell.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention provides automatic cell classification-f! Regarding #, the present invention relates to an automatic cell sorting device suitable for a technician or the like to re-examine cells that have been blindly observed in *.

[Conventional technology]

Using an automatic cell sorter, a doctor or laboratory technician can determine the percentage of blood cells in the foot? The number of Haya R that can be re-examined is 1, for example, the Tokuko Sho 60-
8460. Does this feature only position certain cells on the specimen during automatic classification? This is a power theft that is stored in memory device #, and upon reexamination, the tlJP podocytes are pulled out under a microscope and can be visually observed.

[Problem that the invention seeks to solve]

The above-mentioned conventional technology memorizes the level of specific cells on the sample during automatic classification, so when the technician performs re-examination after automatic classification, he or she must change the cell strain to be re-examined. There is a problem in that if the need arises, it is necessary to re-correct the automatic classification P or V.

An object of the present invention is to provide an automatic cell classification device that can change the aiI class 3 of cells to be retested without having to re-classify cells or repeat the 9th shift.

[Means for solving the problem] The above purpose is achieved by storing the positions on the specimen of all cells for which white classification is to be performed, instead of storing only specific cells during automatic classification. .

[Effect]

The automatic cell classification system according to the present invention stores in a storage device the examination order number of the slide specimen and the positions on the slide specimen of all automatically classified cells during automatic cell classification. Therefore, it is possible to visually observe any type of cell using a microscope when performing a re-examination after the automatic classification is completed, and even if the cell type is changed during the re-examination, it can be observed without re-executing the automatic classification. .

〔Example〕

Hereinafter, an example 1f of the present invention will be explained with reference to the drawings.

The second FA is the main part configuration of the cell white sand sorting device according to one embodiment of the present invention, and Figure I@3 is a block diagram of the antibacterial system of this device.

A plurality of sample force sets 2vC#'i are stored on the turntable l (not shown). The motor 7 table l is the upper and lower motor (
(not shown) and the rotation motor 3.

Sequentially move the slide specimens to the slide transport mechanism 4 at position #
It is controlled by the control device 24 to move up to 12. The slide specimen at movement position #12 VC is pushed onto the slide transport mechanism 4 by the extrusion mechanism 5, and the slide transport mechanism 4 moves the slide specimen to the supply position #11 to the microscope stage arm 6 by the motor of the extrusion mechanism 5. . The microscope stage arm 6 is moved by the motors 7 and 8 from the slide sample supply position @11 to the field of view of the microscope steel 9 by 1 kJJζ using a slide holding mechanism (not shown).

When the movement is completed T, the cell image detected by the cell detection device 11F20 and magnified by the microscope 91C is input to the image processing device 22 by the photoelectric conversion element 21.

The image processing device 22 performs automatic classification of each cell, and stores the classification result of each observed cell, the position 11 of the cell on the specimen, and the corresponding slide examination order number sequentially, and performs automatic classification. The above operation is repeated until the number reaches a predetermined number, for example 100. During this time, cell images are sequentially displayed on the l[ii1 image display device f127vc.

Meanwhile, the slide transport mechanism 4 is automatically sorting! /c $#lL to transfer position 12, slide specimen to be classified next to extruder! 5, push it out to the rainbow slide conveyance mechanism 4 and move it to the standby position #
Move the slide sample up to number 10 and wait for the automatic classification of the previous slide sample to finish. When the automatic classification is completed, the specimen that has been classified (V&F stay 7 arm 6Fi) is supplied to position 1.
1, and then the slide transport mechanism 4 returns to the standby position.
It moves from O to the supply position 11, removes the specimens that have been classified one time, and holds the specimens to be classified next. Thereafter, the slide conveyance mechanism 4 moves to the transfer position #12, and the slide storage mechanism 26 stores the classified specimen at the position # of the specimen cassette 20.

Hereafter, repeat step 1 until the classification of all the slide specimens on the turntable is completed.

When all the specimens in the specimen cassette 2 yen have been classified,
What type of cells does the technician need to reexamine? Data console 2
Enter by 8. System #1 [Is 1124 the type of cell that was created by humans? Based on this, the automatic classification results stored in the conventional order are read out from the memory device@23, and it is checked whether or not the corresponding cells exist. If there are, the corresponding specimen is moved under the microscope field of view using the same procedure as above. Then, select the relevant cell from the cell position information in the memory device @23, and move it to the stage arm 6.
The stage moving mechanism 25 is used to position the cell, and the cell in question can be visually observed using the microscope 9vc, and the technician inputs the cell determination result using the data console 28. After inputting, if there are roughly corresponding specimens on the same specimen, repeat the same procedure, and when all the manual labor for the relevant cells is completed, visually observe the slide specimen containing the first relevant cells using the same method as above. repeat.

Cell σ1# treatment (smearing) was performed during the re-examination described above.

When re-examining a specimen that requires precise re-examination in advance, such as a T-9 or hospitalized patient, where a Vc abnormality is discovered (drying, staining, etc.), the conditions for the types of cells to be re-examined are automatically classified. In this case, the automatic classification may be different without running the automatic classification again.

It is possible to re-inspect by changing the specification.

A 7CI-chart of the embodiment of the present invention described above is shown in FIG.

According to the unexploited site, even if the designation conditions for cells that require recalibration are changed after automatic classification is executed, recalibration can be performed without executing automatic classification again.

[Effects of the Invention] According to the present invention, the KT stores all cell classification results and positions on the slide during automatic cell classification.

When performing a reexamination, it becomes possible to specify any type of cell after automatic classification, which has the effect of improving the efficiency of testing and improving the reliability of the examination results.

[Brief explanation of drawings]

＠l□□□ shows the operation of the device shown in Fig. 2, which is an embodiment of the present invention. It is a block diagram of a feeding system. l... Turntable, 2... Specimen cassette, 4
...Slide conveyance mechanism, 5...Extrusion mechanism, 6...
Stage arm, 9...Microscope, 12...Transfer position, 20...Cell detection device, 22...Image processing device t
, 23...Memory #. Kaya 1 figure 2nd factor 1-・ter'-1-γ? 5.-Press the carpet 6--Stage 7-frame 1-wfI mirror

Claims (1)

[Claims] 1. An image processing device that photoelectrically converts a microscopic image of cells smeared on a slide specimen and automatically classifies the cells, a slide storage mechanism that stores a plurality of the slide specimens, and a slide storage mechanism that collects cells from the microscope. a slide transport device that automatically supplies the slide specimen to an examination position and stores the slide specimen in the slide storage mechanism after automatic cell classification; and a control that controls operations of the image processing device and the slide storage mechanism. In an automatic cell sorting device equipped with the device, during automatic cell classification, the test order number of the slide specimen, the automatic classification results of all automatically classified cells, and the positions of the cells on the slide specimen are stored in a storage device. , after the automatic classification of all the slide specimens is completed, in response to the designation of a specific cell type, the slide specimens stored in the slide storage mechanism are sequentially supplied to the microscopic cell examination again by the slide transport device; An automatic cell sorting device characterized in that designated cells can be visually observed.