JPS62238449A - Apparatus for detecting co2 concentration in culture box - Google Patents
Apparatus for detecting co2 concentration in culture boxInfo
- Publication number
- JPS62238449A JPS62238449A JP8269586A JP8269586A JPS62238449A JP S62238449 A JPS62238449 A JP S62238449A JP 8269586 A JP8269586 A JP 8269586A JP 8269586 A JP8269586 A JP 8269586A JP S62238449 A JPS62238449 A JP S62238449A
- Authority
- JP
- Japan
- Prior art keywords
- gas
- culture chamber
- temperature
- measured
- temp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 claims description 13
- 238000000034 method Methods 0.000 abstract description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 229920006395 saturated elastomer Polymers 0.000 description 5
- 238000001816 cooling Methods 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000223014 Syzygium aromaticum Species 0.000 description 1
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 210000001217 buttock Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（イ）産業上の利用分野
本発明は癌細胞等の組繊細胞を培養するための培養庫に
於いて培養室内のＣＯ，ガス濃度の表示或いは制御等に
用いられるＣＯ，ガス濃度検出装置に関するものである
。Detailed Description of the Invention (a) Industrial Application Field The present invention is used for displaying or controlling CO and gas concentrations in the culture chamber in a culture chamber for culturing tissue cells such as cancer cells. This relates to a CO and gas concentration detection device.
（ロ）従来の技術
従来此種培養庫では培養室内を人体内の環境に近づける
ために温度は周囲温度よりも高い例えば＋３７°Ｃ（こ
れは人間の体温に等しい。）、湿度は例えば９５乃至９
８％（相対湿度）等の高湿度に維持される。又、Ｃｏ、
（二酸化炭素）ガスを所定量供給する事により所定のＣ
Ｏ，ガス濃度（例えば１０％程）に制御される。(B) Conventional technology In conventional seed culture chambers, in order to make the inside of the culture chamber close to the environment inside the human body, the temperature is higher than the ambient temperature, for example, +37°C (this is equivalent to the human body temperature), and the humidity is, for example, 95 to 95°C. 9
The humidity is maintained at a high level, such as 8% (relative humidity). Also, Co,
By supplying a predetermined amount of (carbon dioxide) gas, a predetermined C
O, gas concentration (for example, about 10%) is controlled.
ここでｃｏ、ガス濃度を制御する為、更には表示する為
にそれを測定する手段として、気体濃度が変化すること
による気体の熱伝導度の変化を検出する事によりＣＯ，
ガス濃度を検出する所謂熱伝導式のＣＯ，ガス検出器を
用いた場合には、第３図及び第４図に示す如く被測定ガ
スの温度と絶対湿度によってその測定値が大きく変化し
てしまう。Here, as a means of measuring the gas concentration in order to control and display it, CO,
When using a so-called thermal conduction type CO gas detector that detects gas concentration, the measured value changes greatly depending on the temperature and absolute humidity of the gas to be measured, as shown in Figures 3 and 4. .
即ち第３図は被測定ガス中のｃｏ、ガス濃度（Ｄ）と検
出器から得られる出力電圧（■。）の関係を示している
。図中直線（ＬＬ）、（Ｌ、）、（Ｌ、）、（Ｌ４）、
（Ｌ６）、（Ｌ、）はそれぞれ被測定ガスの温度（Ｔ）
が０℃、１０℃、２０℃、３０℃、４０℃、５０℃の場
合を表わす。即ち濃度（Ｄ）と出力電圧（■。）とは各
温度に於いて略比例関係を示すが、温度が異なればその
傾きが変化してしまう事を表わしている。That is, FIG. 3 shows the relationship between the co gas concentration (D) in the gas to be measured and the output voltage (■.) obtained from the detector. In the figure, straight lines (LL), (L,), (L,), (L4),
(L6) and (L,) are the temperature (T) of the gas to be measured, respectively.
represents the case where is 0°C, 10°C, 20°C, 30°C, 40°C, and 50°C. That is, the concentration (D) and the output voltage (■.) show a substantially proportional relationship at each temperature, but the slope changes if the temperature differs.
又、第４図には被測定ガスの絶対湿度（ｘ）と出力型Ｅ
Ｅ（ｖｏ）の関係を示している。尚、濃度（Ｄ＞及び温
度（Ｔ）は一定である。図に示された曲線より明らかな
如く、ガスの濃度（Ｄ）及び温度（Ｉ’）が一定であっ
ても絶対湿度（ｘ）が変化すると出力電圧（Ｖ。）が大
きく変化してしまう。In addition, Fig. 4 shows the absolute humidity (x) of the gas to be measured and the output type E.
It shows the relationship of E(vo). Note that the concentration (D> and temperature (T) are constant.As is clear from the curve shown in the figure, even if the concentration (D) and temperature (I') of the gas are constant, the absolute humidity (x) If the voltage changes, the output voltage (V) will change significantly.
更にＣＯ，ガス検出器として、赤外光がガスを通過した
ときの減衰量によって検出する事により濃度を検出する
所謂赤外線式の検出器を用いた場合には、精度は向上す
るものの、装置が大型化して構成も複雑である為に此種
培養庫では実用に供せない。Furthermore, when using a so-called infrared detector that detects the concentration by detecting the amount of attenuation when infrared light passes through the gas as a CO/gas detector, the accuracy is improved, but the equipment is Since it is large and has a complicated structure, it cannot be put to practical use in this type of culture chamber.
これを解決するために従来では例えば特開昭６０−１４
１２７９号公報では恒温槽内から導出した被測定ガスを
冷却装置によって一定温度に冷却し、ガスの絶対湿度を
強制的に飽和絶対湿度にして一定としこれによってＣＯ
□ガス検出器からの出力電圧の温度及び湿度による変動
を防止して正確な測定ができる様にしている。In order to solve this problem, for example, Japanese Patent Application Laid-Open No. 60-14
In Publication No. 1279, a gas to be measured drawn out from a constant temperature chamber is cooled to a constant temperature by a cooling device, and the absolute humidity of the gas is forced to be a saturated absolute humidity and constant, thereby CO
□Prevents fluctuations in the output voltage from the gas detector due to temperature and humidity to ensure accurate measurements.
（ハ）発明が解決しようとする問題点
前述の公報の如き構成によれば被測定ガスの温度及び湿
度を強制的に一定とする事により正確なＣＯ２ガス濃度
の検出が可能となるが、電動圧縮機等の機械部品を具備
した冷却装置を培養庫に取付けなければならず、培養庫
自体の大型化とコストの高騰を引き起こしていた。(c) Problems to be solved by the invention According to the configuration as described in the above-mentioned publication, accurate detection of CO2 gas concentration is possible by forcibly keeping the temperature and humidity of the gas to be measured constant; A cooling device equipped with mechanical parts such as a compressor must be attached to the culture chamber, which increases the size of the culture chamber itself and increases costs.
本発明は斯かる問題点を解決するために成されたもので
ある。The present invention has been made to solve these problems.
（ニ）問題点を解決するための手段
実施例に沿って本発明の詳細な説明すると、培養庫（１
）の室（５）内はヒータ（６）により加熱きれ、又、バ
ットク７）により加湿されて例えば＋３７℃で湿度９５
乃至９８％とされる。培養室（５）内のガスは被測定ガ
スとしてエアーポンプ（２１）により導出されて熱交換
器（１３）を通過せられ、その際周囲温度に冷却される
事によって相対湿度１００％とされる。この被測定ガス
の温度（Ｔ）とＣＯｔガス濃度（Ｄ）を検出する為の温
度検出器（１９）及び熱伝導式のｃｏ、ガス検出器（１
８）を設け、マイクロコンピュータ（２６）によって温
度（Ｔ）より被測定ガスの絶対湿度（Ｘ）を決定させ、
更にＣＯｔガス検出器（１８）の出力電圧（■、）と温
度（Ｔ）及び絶対湿度（ｘ）からＣＯ，ガス濃度（Ｄ）
の正確な値を算出させるものである。(d) Means for Solving the Problems The present invention will be described in detail with reference to embodiments.
) The inside of the chamber (5) is heated by the heater (6), and is humidified by the buttock 7) to a temperature of +37°C and a humidity of 95%.
It is said to be between 98% and 98%. The gas in the culture chamber (5) is drawn out as a gas to be measured by an air pump (21) and passed through a heat exchanger (13), where it is cooled to ambient temperature and brought to a relative humidity of 100%. . A temperature detector (19), a thermal conduction type CO, and a gas detector (1) are used to detect the temperature (T) and COt gas concentration (D) of this gas to be measured.
8), and the microcomputer (26) determines the absolute humidity (X) of the gas to be measured from the temperature (T),
Furthermore, CO and gas concentration (D) are determined from the output voltage (■, ) of the COt gas detector (18), temperature (T), and absolute humidity (x).
This allows the calculation of accurate values.
（＊）作用
本発明によれば培養室内の湿度が高く、温度も周囲温度
よりも高い点に着目し、大がかりな冷却装置を用いずに
、被測定ガスの相対湿度を１００％として、それにより
飽和絶対湿度の値を決定できるので、被測定ガスの温度
とＣＯ，ガス濃度検出器の出力電圧からＣＯ，ガス濃度
の正確な値を算出できる。(*) Effect According to the present invention, focusing on the fact that the humidity in the culture chamber is high and the temperature is higher than the ambient temperature, the relative humidity of the gas to be measured is set to 100% without using a large-scale cooling device. Since the value of saturated absolute humidity can be determined, accurate values of CO and gas concentrations can be calculated from the temperature of the gas to be measured and the output voltage of the CO and gas concentration detectors.
（へ）実施例
次に図面により実施例を説明する。第１図に於いて、（
１）は此種組繊細胞の培養実験用の培養庫であり、（２
）は断熱箱体、（３）はこの断熱箱体く２）内に間隔（
４）を存して設けた内箱で内部に培養室（５）を形成す
る。間隔（４）内にはヒータ（６）が配設され、間隔（
４）内の空気をこのヒータ（６）によって加熱する事に
より培養室（５）内は内箱（３〉周壁より間接的に加熱
される事になる。ここでヒータ（６）の発熱量は培養室
（５）内の温度に基づき図示しない温度制御回路が調節
する事により、培養室（５）内は例えば＋３７’Ｃ一定
とされる。又、培養室（５）内には加湿用の水を入れた
バット（７）が収納配設され、この水が蒸発する事によ
り培養室（５）内は相対湿度で９５乃至９８％の高湿度
とされている。（９〉はＣＯ！ （二酸化炭素）を封入
したボンベであり、電磁弁（１０）を介設されたＣＯ，
ガス輸送管（１１）によって培養室（５）内にｃｏ２ガ
スを供給するものである。(f) Example Next, an example will be explained with reference to the drawings. In Figure 1, (
1) is a culture chamber for culturing experiments of this kind of tissue cell, and (2)
) is a heat insulating box, (3) is a heat insulating box.
4), a culture chamber (5) is formed inside the inner box. A heater (6) is arranged within the interval (4), and a heater (6) is provided within the interval (4).
4) By heating the air inside the culture chamber (5) with this heater (6), the inside of the culture chamber (5) will be heated indirectly from the inner box (3) peripheral wall.Here, the amount of heat generated by the heater (6) is A temperature control circuit (not shown) adjusts the temperature in the culture chamber (5) based on the temperature in the culture chamber (5), so that the temperature inside the culture chamber (5) is kept constant at, for example, +37'C.In addition, there is a humidifier in the culture chamber (5). A vat (7) containing water is stored, and as this water evaporates, the inside of the culture chamber (5) is kept at a high relative humidity of 95 to 98%. (9> is CO! It is a cylinder filled with CO2 (carbon dioxide), and a solenoid valve (10) is installed.
CO2 gas is supplied into the culture chamber (5) through a gas transport pipe (11).
次に（１２）はＣＯ□ガス濃度検出装置であり、断熱箱
体（２）外部に取り付けられる。（１３）は例えば熱良
導性の金属パイプから成る熱交換器であり、垂直方向に
配設される。（１４）は培養室（５）と熱交換器（１３
〉下部の分岐点（Ｐ）とを連通ずる導出管であり、熱交
換器（１３）下端は排水皿（１５）内の水に没入されて
液封される。熱交換器（１３）は培養庫（１）の据え付
けられた周囲の空気と良好に熱交換できる様に設けられ
、更に所定の長きを有しており、上部に連通して検出室
（１７）が形成されている。検出室（１７）内には前述
の熱伝導式のｃｏ、ガス検出器（１８）と半導体素子か
ら成り温度によってその抵抗値が変化する温度検出器（
１９）が収納配設され、ここを通過するガス中に晒され
る。検出室（１９）上部と培養室（５）内は帰還管（２
０）によって連通され、帰還管（２０）にはエアーポン
プ（２１）が介設されている。このエアーポンプ（２１
）が駆動する事によって培養室（５）内の少量のガスが
被測定ガスとして導出管（１４）により熱交換器（１３
）下部に導かれ、熱交換器（１３）内を上昇する。被測
定ガスは熱交換器（１３）内を上昇する過程で周囲温度
と熱交換する。ここで熱交換器（１３）の長さはガスが
周囲空気と十分熱交換して略周囲温度となる様に予め設
定しておく。又、培養室（５）内の被測定ガスの温度は
３７°Ｃであり周囲温度より高く、又、湿度は９５％以
上等の高い値であるので、熱交換器（１３）を通過する
際に熱交換器（１３）によって冷却され、相対湿度は１
００％とされた後、検出室（１７）に流入する。この時
凝縮した余剰水分は排水皿（１５）に落下する。検出室
（１７）を通過したガスは帰還管く２０）より室（５）
内に戻る。Next, (12) is a CO□ gas concentration detection device, which is attached to the outside of the heat insulating box (2). (13) is a heat exchanger made of, for example, a metal pipe with good thermal conductivity, and is arranged vertically. (14) is the culture chamber (5) and the heat exchanger (13).
>This is a lead-out pipe that communicates with the lower branch point (P), and the lower end of the heat exchanger (13) is immersed in water in the drain tray (15) and is sealed with liquid. The heat exchanger (13) is provided so as to be able to exchange heat well with the air surrounding the culture chamber (1), and has a predetermined length, and communicates with the upper part of the detection chamber (17). is formed. Inside the detection chamber (17) is the aforementioned thermal conduction type CO, a gas detector (18), and a temperature detector (18) consisting of a semiconductor element whose resistance value changes depending on the temperature.
19) is housed and exposed to the gas passing through it. The upper part of the detection chamber (19) and the inside of the culture chamber (5) are equipped with a return pipe (2).
0), and an air pump (21) is interposed in the return pipe (20). This air pump (21
) is driven, a small amount of gas in the culture chamber (5) is transferred to the heat exchanger (13) as a gas to be measured via the outlet pipe (14).
) is guided to the bottom and rises inside the heat exchanger (13). The gas to be measured exchanges heat with the ambient temperature in the process of rising inside the heat exchanger (13). Here, the length of the heat exchanger (13) is set in advance so that the gas can sufficiently exchange heat with the surrounding air to reach approximately ambient temperature. In addition, the temperature of the gas to be measured in the culture chamber (5) is 37°C, which is higher than the ambient temperature, and the humidity is high, such as 95% or more, so when passing through the heat exchanger (13), is cooled by a heat exchanger (13), and the relative humidity is 1
After being set to 00%, it flows into the detection chamber (17). Excess moisture condensed at this time falls into the drain tray (15). The gas that has passed through the detection chamber (17) is sent to the chamber (5) via the return pipe 20).
Go back inside.
ここで第３図及び第４図に示す如き特性を有するＣＯ，
ガス検出器（１８）の出力電圧（Ｖ、）は例えば 、次
に示す近似式によって表わす事がてきる。Here, CO having the characteristics as shown in FIGS. 3 and 4,
The output voltage (V, ) of the gas detector (18) can be expressed, for example, by the following approximate equation.
Ｖｏキａ （Ｄ−ｃｘ）（ａ−ｂＩ＋ｄＴ”） ＝＝
■ここでα、ａ、ｂ、ｃ、ｄは比例定数。Ｔは温度（
°Ｃ）、ＤはＣＯ，ガス濃度（％）、Ｘは絶対湿度（ｇ
／ｍ”）である。この式■を変形すれば下記の如く被測
定ガス中のＣＯ，ガス濃度（Ｄ）をガスの温度（Ｔ）と
絶対湿度（Ｘ）で表わす事ができる。Voki a (D-cx) (a-bI+dT”) ==
■Here, α, a, b, c, and d are proportionality constants. T is temperature (
°C), D is CO, gas concentration (%), and X is absolute humidity (g
/m"). By transforming this equation (2), the CO and gas concentration (D) in the gas to be measured can be expressed in terms of the gas temperature (T) and absolute humidity (X) as shown below.
■
Ｄ：１Ｔ筐廿−ｗ丁子ｃｘ ・・・・・・・・・・・
・■ここで検出室（１７）内に流入する被測定ガスは前
述の如く相対湿度１００％とされている。相対湿度１０
０％のガスの絶対湿度（ｘ）は飽和絶対湿度であり、各
温度に対する飽和絶対湿度は一般に確められている。従
って被測定ガスの温度（Ｉ’）は温度検出器（１９）に
て測定できるため、絶対湿度（ｘ）も決定される。これ
によってｃｏ、ガス検出器（１８）より得られる出力電
圧（ｖｏ）によって被測定ガス中のＣｏ２ガス濃度を正
確に決定する事ができる。■ D: 1T cabinet-w clove cx ・・・・・・・・・・・・
-■Here, the gas to be measured flowing into the detection chamber (17) has a relative humidity of 100% as described above. relative humidity 10
The absolute humidity (x) of a gas at 0% is the saturated absolute humidity, and the saturated absolute humidity for each temperature is generally determined. Therefore, since the temperature (I') of the gas to be measured can be measured by the temperature detector (19), the absolute humidity (x) can also be determined. Thereby, the Co2 gas concentration in the gas to be measured can be accurately determined based on the output voltage (vo) obtained from the co and gas detector (18).
第２図に培養庫（１）の培養室（５）内ＣＯ！ガス濃度
を算出し、制御するための制御回路（２３）をブロック
図で示す。ＣＯ，ガス検出器（１８）の出力電圧は増幅
器（２４）にて増幅した後Ａ／Ｄ変換器（２５）でディ
ジタル信号に変換きれて半導体から成る凡用のマイクロ
コンピュータ（２６）に入力される。温度検出器（１９
）の出力電圧も増幅器（２７）とＡ／Ｄ変換器（２８）
を介してマイクロコンピュータ（２６）に入力される。Figure 2 shows the CO in the culture room (5) of the culture warehouse (1)! A control circuit (23) for calculating and controlling gas concentration is shown in a block diagram. The output voltage of the CO gas detector (18) is amplified by an amplifier (24), converted into a digital signal by an A/D converter (25), and inputted to a general-purpose microcomputer (26) made of semiconductor. Ru. Temperature detector (19
) output voltage also depends on the amplifier (27) and A/D converter (28).
The signal is inputted to the microcomputer (26) via the microcomputer (26).
（２９）は培養室（５）内のＣＯ，ガス濃度の設定装置
で例えば０％から２０％まで設定でき、その出力はマイ
クロコンピュータ（２６）に入力されている。マイクロ
コンピュータ（２６）はＣＯ，ガス検出器（１８）の出
力電圧（ｖｏ）と温度検出器（１９）からの温度（Ｔ）
に基づく情報により、予め記憶された温度（１）に対す
る飽和絶対湿度の値から絶対湿度（ｘ）を算出し、前述
の式■に基づいて、弐〇の処理を実行し、ＣＯ，ガス濃
度（Ｄ）を算出する。この算出値に基づいて設定装置（
２９〉からの設定値に近づける様電磁弁（１０）に出力
を発生して開閉制御し、培養室（５）内のｃｏ、ガス濃
度（Ｄ）を一定に制御する。(29) is a CO and gas concentration setting device in the culture chamber (5), which can be set, for example, from 0% to 20%, and its output is input to the microcomputer (26). The microcomputer (26) detects CO, the output voltage (vo) of the gas detector (18), and the temperature (T) from the temperature detector (19).
Based on the information based on , absolute humidity (x) is calculated from the value of saturated absolute humidity for temperature (1) stored in advance, and based on the above formula D) is calculated. Based on this calculated value, the setting device (
An output is generated to the electromagnetic valve (10) to control opening and closing so as to approach the set value from 29>, thereby controlling the CO and gas concentration (D) in the culture chamber (5) to be constant.
更に前述の算出値により表示器（３０）に現在のＣＯ、
ガス濃度（Ｄ）を表示する。Furthermore, the display (30) displays the current CO and
Displays gas concentration (D).
（ト）発明の効果
本発明によれば培養室内を周囲温度よりも高く、高湿度
に維持される培養庫内のＣＯ，ガス濃度を簡単な構成で
正確に検出する事ができる。この時被測定ガスの湿度を
一定にするための冷却装置等も不要であり実用的効果の
大なるものである。(G) Effects of the Invention According to the present invention, it is possible to accurately detect CO and gas concentrations in the culture chamber, where the temperature inside the culture chamber is kept higher than the ambient temperature and high humidity, with a simple configuration. At this time, there is no need for a cooling device or the like to keep the humidity of the gas to be measured constant, and this has a great practical effect.
各図は本発明の実施例を示すもので、第１図は培養庫及
びＣＯ，ガス濃度検出装置の構成を示す図、第２図は制
御回路のブロック図、第３図は被測定ガス中のＣＯ，ガ
ス濃度と検出器の出力電圧の関係を示す図、第４図は被
測定ガスの絶対湿度と検出器の出カ寛圧の関係を示す図
である。
（１）・・・培養庫、 （５）・・・培養室、 （９）
・・・ボンベ、 （１２）・・・ＣＯ１ｉ ス濃＆検出
装ｆｉｔ、 （１３）−０゜熱交換器、 （１８）・・
・ｃｏ、ガス検出器、 （１９）・・・温度検出器、（
２１）・・・エアーポンプ、 （２３）・・・制御回路
、（２６）・・・マイクロコンピュータ。
出願人 三洋電機株式会社外１名
代理人 弁理士 西野卓嗣 外１名
＠１図
Ｚ９ ２５
第４図
第３図Each figure shows an embodiment of the present invention. Figure 1 is a diagram showing the structure of the culture chamber and CO and gas concentration detection device, Figure 2 is a block diagram of the control circuit, and Figure 3 is a diagram showing the configuration of the culture chamber and CO and gas concentration detection devices. FIG. 4 is a diagram showing the relationship between the CO and gas concentration and the output voltage of the detector, and FIG. 4 is a diagram showing the relationship between the absolute humidity of the gas to be measured and the output tolerance pressure of the detector. (1)...Cultivation warehouse, (5)...Cultivation room, (9)
...Cylinder, (12)...CO1i concentration & detection device fit, (13)-0° heat exchanger, (18)...
・co, gas detector, (19)...temperature detector, (
21)...Air pump, (23)...Control circuit, (26)...Microcomputer. Applicant Sanyo Electric Co., Ltd. and one other agent Patent attorney Takuji Nishino and one other person @1 Figure Z9 25 Figure 4 Figure 3
Claims (1)
る培養庫に於いて、前記培養室内の被測定ガスを庫外に
導き周囲温度と熱交換せしめる熱交換器と、該熱交換器
を通過した前記被測定ガス中に晒される温度検出器及び
熱伝導式ＣＯ＿２ガス検出器と、両検出器の出力に基づ
き前記培養室のＣＯ＿２ガス濃度を算出する制御装置と
を具備して成る培養庫のＣＯ＿２ガス濃度検出装置。1. In a culture chamber in which the culture chamber is maintained at a temperature higher than the ambient temperature and at high humidity, a heat exchanger that guides the gas to be measured in the culture chamber to the outside and exchanges heat with the ambient temperature; and the heat exchanger. A culture comprising: a temperature detector and a thermal conduction CO_2 gas detector that are exposed to the gas to be measured that has passed through; and a control device that calculates the CO_2 gas concentration in the culture chamber based on the outputs of both detectors. CO_2 gas concentration detection device in the warehouse.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8269586A JPS62238449A (en) | 1986-04-10 | 1986-04-10 | Apparatus for detecting co2 concentration in culture box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8269586A JPS62238449A (en) | 1986-04-10 | 1986-04-10 | Apparatus for detecting co2 concentration in culture box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62238449A true JPS62238449A (en) | 1987-10-19 |
Family
ID=13781545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8269586A Pending JPS62238449A (en) | 1986-04-10 | 1986-04-10 | Apparatus for detecting co2 concentration in culture box |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62238449A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04502690A (en) * | 1988-12-12 | 1992-05-14 | カーティス インスツルメンツ インコーポレイテッド | Inductance measuring device and method |
| US6255103B1 (en) * | 1998-09-29 | 2001-07-03 | Sanyo Electric Co., Ltd. | Cultivating apparatus with sterilizing lamp |
| GB2495934A (en) * | 2011-10-25 | 2013-05-01 | Tap Biosystems Phc Ltd | Bioreactor outlet air conditioning systems and associated methods |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60141279A (en) * | 1983-12-28 | 1985-07-26 | Sanyo Electric Co Ltd | Thermostatic chamber |
-
1986
- 1986-04-10 JP JP8269586A patent/JPS62238449A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60141279A (en) * | 1983-12-28 | 1985-07-26 | Sanyo Electric Co Ltd | Thermostatic chamber |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04502690A (en) * | 1988-12-12 | 1992-05-14 | カーティス インスツルメンツ インコーポレイテッド | Inductance measuring device and method |
| US6255103B1 (en) * | 1998-09-29 | 2001-07-03 | Sanyo Electric Co., Ltd. | Cultivating apparatus with sterilizing lamp |
| US6593131B2 (en) | 1998-09-29 | 2003-07-15 | Sanyo Electric Co., Ltd. | Cultivating apparatus with sterilizing lamp |
| GB2495934A (en) * | 2011-10-25 | 2013-05-01 | Tap Biosystems Phc Ltd | Bioreactor outlet air conditioning systems and associated methods |
| US9382511B2 (en) | 2011-10-25 | 2016-07-05 | Tap Biosystems (Phc) Limited | Bioreactor outlet air conditioning systems and associated methods |
| US10844341B2 (en) | 2011-10-25 | 2020-11-24 | The Automation Partnership (Cambridge) Ltd. | Bioreactor outlet air conditioning systems and associated methods |
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