JPS62238214A - Bathing agent - Google Patents

Abstract

PURPOSE:A bathing agent which is simply soluble in warm water, shows digesting action on stain of protein and can keep long-term activity of protease, obtained by blending glycerin as a main component with protease and an organic acid anhydride, etc., as an enzyme stabilizer. CONSTITUTION:Glycerin as a main component is blended with protease and an organic acid anhydride, inorganic acid anhydride, inorganic salt or a mixture thereof as an enzyme stabilizer. A protease derived from Bacillus subtilis is used as the protease and the amount of it used is 0.1-1.2g in 100ml bathing agent. Citric anhydride as the organic anhydride, silicic anhydride as the inorganic anhydride and anhydrous borax as the anhydrous inorganic salt may be cited as the enzyme stabilizer.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention contains glycerin as a main component, protease,
The present invention also relates to a protease-containing bath agent with improved enzyme stability, which contains anhydrous organic acid, anhydrous inorganic acid, anhydrous inorganic salt, or a mixture thereof as an enzyme stabilizer.

[Problems to be solved by conventional technology and invention]

It has been known for some time that proteases are added to bath preparations in order to digest protein stains.1 Examples of bath preparations containing such proteases include Kansai Koso (Rupert Enzyme). However, since all of these protease-containing bath preparations are in the form of powder or solid, they must be dissolved before use. Furthermore, even after melting, it often appears that the substance has melted, but in reality it is simply dispersed or precipitated in the hot water. Furthermore, the water content of the smelt was relatively low, so the enzyme titer was unstable.

There is also no known protease kinpaku bath agent in liquid form.

``Means to Solve the Problems'' As a result of intensive 4i11 research to solve the problems of Sou-L and create a liquid form of PROTE bath agent 30゛ bath agent, we found that the main ingredient was glycerin. By blending component 12 with anhydrous organic acid, anhydrous inorganic acid, anhydrous inorganic salt, or a mixture thereof as a protease and enzyme stabilizer, the protease-containing bath agent can be changed to a liquid form and the activity of the protease can be preserved for a long period of time. They discovered stabilized scales and completed the present invention.

[Example] The protease-containing bath agent of the present invention has glycerin as its main component, and the protease is
It contains 0.1 to 1.2 g.

In addition to its skin-smoothing effect as a bath agent, glycerin also has an excellent stabilizing effect on enzymes. The reason why glycerin does not have a stabilizing effect on enzymes is because hydrogen bonds are formed between glycerin and enzymes, and glycerin surrounds the enzymes, stabilizing enzyme activity. This is thought to be due to In this way, glycerin alone has an enzyme stabilizing effect, so the b[j-tease in bath preparations is stabilized. However, since glycerin has a purity of about 98%, the water content in glycerin Enzyme activity is not stable over a long period of time, and changes in enzyme activity over time are observed. In order to further stabilize protease in bath preparations for a longer period of time, the present inventors added 9-acid anhydride, 9-acid anhydride, and
When anhydrous inorganic acid, anhydrous inorganic salt, or a mixture thereof was added, the enzyme stability was dramatically improved, and the stability of the enzyme was 75% at 40°C.
We found that the enzyme activity remained almost stable for two months under (humidity) conditions. Such a stabilizing effect is thought to be due to the fact that these enzyme stabilizers convert reactive water contained in glycerin into crystalline non-reactive water.

Specific examples of enzyme stabilizers include anhydrous organic acids and anhydrous citric acid, anhydrous inorganic acids such as silicic anhydride, and anhydrous inorganic salts such as anhydrous borax, anhydrous mirabilite, anhydrous calcium oxide, and anhydrous calcium carbonate. etc. can be given.

The protease-containing bath preparation of the present invention has various effects as a bath preparation, including recovery from fatigue, stiff shoulders, neuralgia, lower back pain, chilblains, sensitivity to cold, skin irritation, heat rash, jock itch, eczema, ringworm, bruises, sprains, and rheumatism. It is effective for hemorrhoids, acne, athlete's foot, cracks, chapped bones, and medical problems.

The bath preparation of the present invention has an amount of 10 to 30 m1/20 Jl per day.
Can be used in two bathtubs.

The protease used in the bath preparation of the present invention is Bacillus subtilis.
A proteolytic enzyme derived from Bacillus subtilis is used.

Commercially available products include Proleather (manufactured by Ohno Pharmaceutical ■), Protease N (manufactured by Amano Corporation $■), and Protease Conc (manufactured by Nagase Sangyo ■).

Next, the present invention will be explained in more detail using Examples and Test Examples, but the present invention is not limited to these.

Example 1 A bath preparation consisting of the following ingredients was manufactured.

Glycerin 95.0H Protease 0.2g (Proleather (manufactured by Ohno Pharmaceutical)) Citric acid anhydride 0.5g Uranine 0.2g Fragrance Appropriate Total amount 10
0ml Example 2 A bath agent was produced in the same manner as in Example 1 except that silicic anhydride was used instead of anhydrous citric acid.

Example 3 A bath additive was produced in the same manner as in Example 1 except that anhydrous borax was used instead of anhydrous citric acid.

Example 4 A °C bath agent was produced in the same manner as in Example 1, except that anhydrous sodium sulfate was used instead of anhydrous citric acid.

Example 5 A bath additive was produced in the same manner as in Example 1 except that anhydrous calcium carbonate was used instead of anhydrous citric acid.

Test Example 1 To examine enzyme stability, the residual enzyme titer was measured after storage at 37°C for 30 days. The results are shown in Table 1.

[Blank below] Test Example 2 The efficacy of the bath preparation of the present invention was investigated on 30 male and 30 adults, and the results shown in Table 2 were obtained. Furthermore, the second
The results shown in the table are the results obtained when the bath preparation of the present invention was used for one week.

[Margins below] [Effects of the Invention] Since the protease-containing bath agent of the present invention is a liquid, unlike a powder, it dissolves in hot water very easily.

Furthermore, when adding it to hot water, it can be added directly like shampoo or conditioner without tearing the wrapping paper, which is the case with tablets and powders.

Furthermore, since enzyme stability is improved, it is possible to maintain protease activity for a long period of time.

Claims (1)

[Scope of Claims] 1. A protease-containing bath agent with improved enzyme stability, comprising glycerin as a main component, protease, and an anhydrous organic acid, an anhydrous inorganic acid, an anhydrous inorganic salt, or a mixture thereof as an enzyme stabilizer. . 2. The protease-containing bath agent according to claim 1, wherein the anhydrous organic acid is anhydrous citric acid. 3. The protease-containing bath agent according to claim 1, wherein the inorganic acid anhydride is silicic anhydride. 4. The protease-containing bath preparation according to claim 1, wherein the anhydrous inorganic salt is selected from the group consisting of anhydrous borax, anhydrous mirabilite, anhydrous calcium oxide, and anhydrous calcium carbonate.