JPS62231163A - Column for liquid chromatography and its production - Google Patents
Column for liquid chromatography and its productionInfo
- Publication number
- JPS62231163A JPS62231163A JP61073946A JP7394686A JPS62231163A JP S62231163 A JPS62231163 A JP S62231163A JP 61073946 A JP61073946 A JP 61073946A JP 7394686 A JP7394686 A JP 7394686A JP S62231163 A JPS62231163 A JP S62231163A
- Authority
- JP
- Japan
- Prior art keywords
- column
- packer
- liquid chromatography
- filter
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004811 liquid chromatography Methods 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title claims description 6
- 238000012856 packing Methods 0.000 claims abstract description 31
- 239000002245 particle Substances 0.000 claims abstract description 21
- 239000000463 material Substances 0.000 claims abstract description 16
- 239000006185 dispersion Substances 0.000 claims abstract description 15
- 239000000945 filler Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 10
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 6
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical group [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 abstract description 2
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000000926 separation method Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
この発明は、液体クロマトグラフィー用カラムに関する
。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] This invention relates to a column for liquid chromatography.
［従来の技術］
液体クロマトグラフィーは、試料中の種々の物質を分離
するために広く用いられている。液体クロマトグラフィ
ー用カラムは、中空のカラムと、このカラム内に収容さ
れる充は剤とを有する。従来より、液体クロマトグラフ
ィーの目的に応じて種々の充填剤か用いられているか、
これらは通常、重版のカラム充填機を用いてカラム中に
充填されている。[Prior Art] Liquid chromatography is widely used to separate various substances in a sample. A liquid chromatography column has a hollow column and a filler housed within the column. Conventionally, various packing materials have been used depending on the purpose of liquid chromatography.
These are usually packed into columns using a reprint column packing machine.
［従来技術の欠点］ 従来の液体クロマトグラフィｍｍカラムは。[Disadvantages of conventional technology] Conventional liquid chromatography mm column.
その充填剤のカラム充填−（（か低い、すなわち、カラ
ム中に充填剤があまり密に詰まっていない。このため、
１−分な分離源を得るためにはカラムを長くしなければ
ならず、装置が大型化するという欠点を有している。Column packing of the packing material - (lower, i.e., the packing material is not packed very closely in the column.
In order to obtain a 1-minute separation source, the column must be made long, which has the disadvantage of increasing the size of the apparatus.
［発ＩＩか解決しようとするｌ！’１ｍ点］この発明の
目的は、カラム充填率の高い液体クロマトグラフィー用
カラムを提供し、それによっで、単位容ｖｉ（長さ）当
たりの分８１敞か大きな液体クロマトグラフィー用カラ
ムを提供すること、及びこのようなカラムの製造方法を
提供することである。[I'm trying to solve the problem! '1m point] An object of the present invention is to provide a liquid chromatography column with a high column packing rate, thereby providing a liquid chromatography column with a capacity of 81 minutes per unit volume vi (length). The object of the present invention is to provide a method for manufacturing such a column.
［問題を解決するための手段］
本願発明者らは、カラム充ｆｔ率の大きな液体クロマト
グラフィｍｍカラムを求めて鋭意研究した結果、特殊な
充填方法を採用することにより一定容積の中空カラム中
に充填剤を多量に充填することがてき、カラム充填率を
高めることかてきることを見出して本願発明を完成した
。すなわち、この発明は、中空のカラムと、該カラム内
に充填される液体クロマトグラフィー充填剤とを有する
液体クロマトグラフィー用カラムにおいで、前記カラム
中の前記充填剤のカラム充＠率か５０％以上であること
を特徴とする液体クロマトグラフィー用カラムを提供す
る。また、この発明は、下端部にフィルターか取付けら
れたカラムの上端部にパッカーを接続する工程と、該パ
ッカー及び前記カラムを実質的に垂直に保持した状態で
、媒体中に均一に分散させた充填剤粒子の分散液を前記
パッカーを介して前記カラム中に注入する工程と、バ・
ンカー北部にフィルターを取付け、該フィルターを介し
て媒体をポンプを用いてパッカー及び前記カラム中に圧
入する工程とを含む、カラム充填率が５０％以上の液体
クロマトグラフィー用カラムの製造方法を提供する。[Means for Solving the Problem] As a result of intensive research in search of a liquid chromatography mm column with a large column filling ratio, the inventors of the present application have found that by adopting a special packing method, it is possible to fill a hollow column with a constant volume. The present invention was completed by discovering that it is possible to fill a large amount of the agent and increase the column packing rate. That is, the present invention provides a liquid chromatography column having a hollow column and a liquid chromatography packing material filled in the column, wherein the column filling rate of the packing material in the column is 50% or more. A column for liquid chromatography is provided. The present invention also includes a step of connecting a packer to the upper end of a column with a filter attached to the lower end, and a step of uniformly dispersing the packer and the column in a medium while holding the packer and the column substantially vertically. injecting a dispersion of filler particles into the column through the packer;
Provided is a method for producing a column for liquid chromatography having a column filling rate of 50% or more, the method comprising: installing a filter in the northern part of the tank; and pressurizing a medium through the filter into the packer and the column using a pump. .
［発明の効果］
この発明によると、充填剤のカラム充填率か高い液体ク
ロマトグラフィｍｍカラム及びその製造方法か提供され
る。この発明のカラムはカラム充填率が高いので、従来
のカラムと比較しで、同じ大きさてあればその分離源か
従来のカラムよりも大きい。従っで、一定の分離ｆ＠を
有する液体クロマトクラフィー装置をつくった場合に、
この発明のカラムを用いると従来のカラムを用いた場合
よりも装置を小型化できる。[Effects of the Invention] According to the present invention, a liquid chromatography mm column with a high column packing rate and a method for manufacturing the same are provided. Since the column of the present invention has a high column packing ratio, when compared with a conventional column, the separation source is larger than the conventional column if the size is the same. Therefore, if you create a liquid chromatography device with a constant separation f@,
By using the column of this invention, the device can be made smaller than when using a conventional column.
［発ＩＩの具体的説明］
に述したように、この発Ｉｌｌのカラムは、そのカラム
充填率が５０％以上と高いことを特徴とする。ここで、
カラム充填率とは、カラムの実働内容積（カラム中の充
填剤か充填される容積）から充填剤中の空隙部分の体積
を差し引いたものとカラムの実働内容積との比を百分率
で表わしたものである。この発明のカラムの好ましいｆ
Ｅ様ては。[Specific Description of Series II] As described above, the column of Series II is characterized by a high column filling rate of 50% or more. here,
Column packing ratio is the ratio of the actual internal volume of the column (volume filled with the packing material in the column) minus the volume of voids in the packing material to the actual internal volume of the column, expressed as a percentage. It is something. Preferred f of the column of this invention
Mr. E.
カラム充填率か５５％以上、さらに好ましいｆＥ様では
カラム充填率か６５％以上てあり、さらに好ましい１ム
様ではカラム充填率か７５％以上である。The column filling rate is 55% or more, more preferably the column filling rate is 65% or more in the fE type, and the column filling rate is 75% or more in the more preferable 1M type.
この発明のカラムに用いられる充填剤としては、従来か
ら液体クロマトグラフィーに用いられているいずれの充
填剤をも採用することかてき、例えば水酸アパタイト粒
子を挙げることがてきる。As the packing material used in the column of the present invention, any packing material conventionally used in liquid chromatography may be used, and examples thereof include hydroxyapatite particles.
この発明のカラムは、以下のような方法により製造する
ことかてきる。まず、カラムを準備し、このカラムの下
端部にフィルターを取り付ける。フィルターは通常、フ
ィルター付止金によってカラムに取付けられる。フィル
ターは充填剤粒子を通過させずに媒体だけな通過させる
大きさの孔を多数有するものであり、従来の液体クロマ
トグラフィーカラムに用いられているいずれのものをも
用いることかできる。The column of this invention can be manufactured by the following method. First, prepare a column and attach a filter to the bottom end of this column. Filters are typically attached to the column by filter clasps. The filter has a large number of pores that are large enough to allow only the medium to pass through without allowing the filler particles to pass through, and any filter used in conventional liquid chromatography columns can be used.
次に、このカラムの上端部に例えばユニオンのような接
続部材によっで、パッカーを接続する。パッカーは実質
的にカラム状をしており、その径は例えば充填剤を充填
すべきクロマトグラフィー用カラムと同じである。カラ
ム充填率を１げるためには、パッカーの長さは長い方が
好ましく、例えばクロマトグラフィー用カラムの５倍の
長さを右する。Next, a packer is connected to the upper end of this column by a connecting member such as a union. The packer is substantially column-shaped and has the same diameter as, for example, a chromatography column to be packed with packing material. In order to increase the column packing factor by 1, the length of the packer is preferably long, for example, five times the length of the chromatography column.
一方、充填すべき充填剤粒子を媒体中に均一に分散させ
た充填剤粒子の分散液を準備する。媒体としては、液体
クロマトグラフィーを行なう際に用いられる溶は液が好
ましく、例えばリン酸緩衝液を挙げることかてきる。媒
体中の充填剤粒子の濃度は適宜選択することかてきるが
、水酸アパタイト粒子を充填する場合には例えば４ない
し１０ ｗ／ｖ罵である。充填剤粒子を媒体中に均一に
分散させる操作は、例えば充填剤を媒体中に入れて静か
にかきまぜた後、趙汗波水浴上で行なうことかてきる。On the other hand, a dispersion of filler particles is prepared in which filler particles to be filled are uniformly dispersed in a medium. As the medium, the solution used in liquid chromatography is preferably a liquid, such as a phosphate buffer. The concentration of filler particles in the medium can be selected as appropriate, and is, for example, 4 to 10 w/v when filled with hydroxyapatite particles. The filler particles can be uniformly dispersed in the medium by, for example, placing the filler in the medium, stirring gently, and then performing the operation on a Zhaoban water bath.
次に、前記カラムとパッカーの連結体を実質的に前直に
保持し、パッカーの上端部から前記充填剤粒子−分散液
をカラム中に注入する。注入は速やかに、すなわち、カ
ラム中に分散液を落下させるような感じて行なうのが好
ましい。注入をいつまで続けるかは、分散液中の充填剤
粒子の濃度及びカラムとパッカーの容積に依存するが、
水酸アパタイト粒子の４ないしＬｏｗ／ｖ％分散液を用
い。Next, the column and packer connection is held substantially directly in front of each other, and the filler particle-dispersion liquid is injected into the column from the upper end of the packer. Preferably, the injection is carried out quickly, that is, by dropping the dispersion into the column. How long to continue injection depends on the concentration of filler particles in the dispersion and the volumes of the column and packer.
A 4 to Low/v% dispersion of hydroxyapatite particles was used.
パッカーの長さがカラムの長さの５倍である場合には、
パッカー上端部から分散液が溢れ出るまて注入を続ける
ことか好ましい。If the packer length is 5 times the column length, then
It is preferable to continue pouring until the dispersion overflows from the upper end of the packer.
次にパッカーの上端部にフィルターを取付け、このフィ
ルターを介してパッカー内ひいてはカラム内に媒体なポ
ンプで圧入する。この時の媒体も液体クロマトグラフィ
ーを実施する際に用いられる溶離液が好適であり、通常
上記分散液の媒体と回しものか用いられる。圧入時の圧
力は、その最大時の圧力が５０ｋｇ／ｃ謳２ないし５０
０ｋｇ／ｃ膳２であることか好ましい。この最大圧力の
設定は上下限圧力コントローラーによって行なうことが
できる。圧入時の流速は、カラムの内径や容積、充填剤
の種類に応じて適宜選択することかてき、例えば１．Ｏ
ｍｌ／分ないし２．０ｍｌ／分である。また、充填剤を
均一に充填するために、ポンプとしては無脈動ポンプを
用いることか好ましい。また、圧入は。Next, a filter is attached to the upper end of the packer, and a medium is pumped through the filter into the packer and, ultimately, into the column. The medium at this time is preferably an eluent used in liquid chromatography, and is usually used in combination with the medium for the dispersion. The maximum pressure during press-fitting is 50kg/c to 50kg/c.
It is preferable that it is 0 kg/c meal 2. This maximum pressure can be set using an upper and lower limit pressure controller. The flow rate during pressurization can be selected appropriately depending on the inner diameter and volume of the column, and the type of packing material.For example, 1. O
ml/min to 2.0 ml/min. Further, in order to uniformly fill the filler, it is preferable to use a non-pulsating pump as the pump. Also, press fit.
パッカー上部から圧入された媒体と実質的に同じ体桔の
媒体かカラムの下端から流出するまで続けることか好ま
しい。Preferably, the process continues until substantially the same volume of medium as the medium injected from the top of the packer flows out from the bottom of the column.
次に、カラムをパッカーからとりはずし、カラムに端部
にフィルターを取り付けることによってこの発明の液体
クロマトグラフィｍｍカラムか得られる。Next, the liquid chromatography mm column of the present invention is obtained by removing the column from the packer and attaching a filter to the end of the column.
［発明の実施例］
内径８．５■、長さｌｏｃｍのステンレス製カラムのド
端に、厚さ５終糧のフィルターを有するフィルター付止
金を取り付けた。カラム北端にユニオンを介して内径８
．５■ｍ、 Ｑさ５０ｃ■のカラム状のステンレス製パ
ッカーを取り付けた。カラム及びパッカーをクランプで
垂直に保持した。一方、粒径のメジアンが約２終−の水
酸アパタイト粒子を’１５ｍ１の媒体にＩＤｗ／ｖ％の
濃度になるように加え静かにかきまぜ、ａ音波水浴上に
５秒間載せて粒子・を均＝−に分散させた。用いた媒体
は、５１．７ｇのＮａ２甘し一１２１１□０と、２：１
．４ｇのＮａ１ｌ□ＰＯ，’２１１２０と。[Embodiment of the Invention] A filter stopper having a filter with a thickness of 5 cm was attached to the end of a stainless steel column having an inner diameter of 8.5 cm and a length of 1 cm. Inner diameter 8 through the union at the north end of the column
．． A column-shaped stainless steel packer with a length of 5 mm and a Q of 50 cm was attached. The column and packer were held vertically with clamps. On the other hand, add hydroxyapatite particles with a median particle size of about 2 to 15 ml of medium to a concentration of IDw/v%, stir gently, and place on a sonic water bath for 5 seconds to even out the particles. =-dispersed. The medium used was 51.7 g of Na2 sweetened 1211□0 and 2:1
．． 4g of Na1l□PO,'21120.
Ｏ８旧ＩＩ５．：のＣａＣｌ□・２１１ａＯと、０．５
ｇのＮａＮ、とをへ留水に溶かして全４１を１００ｍ１
としたもののｐｌ＋を０．５ＮＮ ａ ＯＩＩ又は０．
５Ｎ ＩＩｃＩで６．８に調整したものてあつた０分散
液をＭｉ音波水浴から取り出し、パッカーの上端からす
ばやくパッカー及びカラム内に注入した。バ・ンカーの
上端から分散液か溢れ出した後注入をやめ、パッカーの
上端に厚さ５終−のフィルターを有するフィルター付止
金をすばやく取り付けた。次に無脈動ポンプを用い、パ
ッカーの上部に取付けられたフィルターを介してパッカ
ー及びカラム内にＬ記奴体を１．Ｓｍｌ／分の流速で圧
入した。この際、上下限圧カコントロ°−ラーを用いで
、最大時の圧力か５（１ｋｇ／ｃｍ２になるように調整
した。カラムの下端から約４０１１の奴体か流出したと
ころて圧入を止め、カラムをバラカーから取り外してそ
の上端部にト記フィルター付止金を取付け、この発明の
カラムを得た。O8 old II5. : CaCl□・211aO and 0.5
Dissolve g of NaN and in distilled water and make 100ml of total 41
pl+ of 0.5NN a OII or 0.
The prepared 0 dispersion, adjusted to 6.8 with 5N IIcI, was removed from the Mi sonic water bath and quickly injected into the packer and column from the top of the packer. After the dispersion overflowed from the top of the bunker, the injection was stopped and a filter clamp with a 5-mm filter was quickly attached to the top of the packer. Next, using a non-pulsating pump, 1.0% of the L-recording body was introduced into the packer and column through the filter attached to the top of the packer. It was injected under pressure at a flow rate of Sml/min. At this time, the upper and lower limit pressure controllers were used to adjust the maximum pressure to 5 (1 kg/cm2). When about 4011 of the body flowed out from the bottom of the column, the pressure injection was stopped and the column was removed from the barracker and the above-mentioned filter clasp was attached to its upper end to obtain a column of the present invention.
次にこのようにして得たカラムのカラム充填部の体積を
測定したところ２．５２ｓｌてあった。従っで、このカ
ラムのカラム充填率は以下のようにして求めることかて
きる。Next, the volume of the column packed portion of the column thus obtained was measured and found to be 2.52 sl. Therefore, the column packing rate of this column can be determined as follows.
Claims (11)
ロマトグラフィー充填剤とを有する液体クロマトグラフ
ィー用カラムにおいて、前記カラム中の前記充填剤のカ
ラム充填率が５０％以上であることを特徴とする液体ク
ロマトグラフィー用カラム。(1) A liquid chromatography column having a hollow column and a liquid chromatography packing material packed into the column, characterized in that the column filling rate of the packing material in the column is 50% or more. Column for liquid chromatography.
１項記載のカラム。(2) The column according to claim 1, wherein the packing rate is 55% or more.
２項記載のカラム。(3) The column according to claim 2, wherein the packing rate is 65% or more.
３項記載のカラム。(4) The column according to claim 3, wherein the packing rate is 75% or more.
の範囲第１項ないし第４項のいずれか１項に記載のカラ
ム。(5) The column according to any one of claims 1 to 4, wherein the filler is hydroxyapatite particles.
部にパッカーを接続する工程と、該パッカー及び前記カ
ラムを実質的に垂直に保持した状態で、媒体中に均一に
分散させた充填剤粒子の分散液を前記パッカーを介して
前記カラム中に注入する工程と、パッカー上部にフィル
ターを取付け、該フィルターを介して媒体をポンプを用
いてパッカー及び前記カラム中に圧入する工程とを含む
、カラム充填率が５０％以上の液体クロマトグラフィー
用カラムの製造方法。(6) Connecting a packer to the upper end of the column with a filter attached to the lower end, and dispersing filler particles uniformly in the medium while holding the packer and the column substantially vertically. injecting a dispersion of the liquid into the column through the packer, attaching a filter to the top of the packer, and pressurizing the medium through the filter into the packer and the column using a pump. A method for producing a column for liquid chromatography having a packing rate of 50% or more.
ｇ／ｃｍ＾２ないし５００ｋｇ／ｃｍ＾２である特許請
求の範囲第６項記載の製造方法。(7) The pressure at the time of press-fitting is 50k at its maximum.
The manufacturing method according to claim 6, wherein the weight is from g/cm^2 to 500 kg/cm^2.
第６項又は第７項記載の方法。(8) The method according to claim 6 or 7, wherein the pump is a non-pulsating pump.
体の体積と実質的に同体積の媒体がカラムの下端から流
出するまで続けられる特許請求の範囲第６項ないし第８
項のいずれか１項に記載の方法。(9) The press-in is continued until substantially the same volume of medium as the volume of the medium press-in from the upper part of the packer flows out from the lower end of the column.
The method described in any one of paragraphs.
て前記カラム中に注入する工程は、該分散液が前記パッ
カーの上部から溢れ出すまで続けられる特許請求の範囲
第６項ないし第９項のいずれか１項に記載の方法。(10) The step of injecting the dispersion of filler particles into the column through the packer is continued until the dispersion overflows from the top of the packer. The method according to any one of the above.
許請求の範囲第６項ないし第１０項のいずれか１項に記
載の方法。(11) The method according to any one of claims 6 to 10, wherein the filler particles are hydroxyapatite particles.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61073946A JPS62231163A (en) | 1986-03-31 | 1986-03-31 | Column for liquid chromatography and its production |
| EP87104683A EP0239970A3 (en) | 1986-03-31 | 1987-03-30 | Assemblage of hydroxyl apatite particles and liquid chromatography column using the same |
| AU70922/87A AU599366B2 (en) | 1986-03-31 | 1987-03-31 | Assemblage of hydroxyl apatite particles and liquid chromatography column using the same |
| CN87102573.6A CN1010746B (en) | 1986-03-31 | 1987-03-31 | Assemblage of hydroxyl apatite particles and liquid chromatography column using the same |
| CA000533469A CA1283395C (en) | 1986-03-31 | 1987-03-31 | Assemblage of hydroxyl apatite particles and liquid chromatography column using the same |
| US07/549,558 US5037543A (en) | 1986-03-31 | 1990-07-09 | Assemblage of hydroxyl apatite particles and liquid chromatography column using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61073946A JPS62231163A (en) | 1986-03-31 | 1986-03-31 | Column for liquid chromatography and its production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62231163A true JPS62231163A (en) | 1987-10-09 |
Family
ID=13532773
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61073946A Pending JPS62231163A (en) | 1986-03-31 | 1986-03-31 | Column for liquid chromatography and its production |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62231163A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019187585A1 (en) * | 2018-03-30 | 2019-10-03 | 株式会社カネカ | Production method for packed bed that comprises polysaccharide column carrier packed into large column by pulsation suppression |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56151352A (en) * | 1980-04-26 | 1981-11-24 | Asahi Chem Ind Co Ltd | Column for gel permeation chromatography and manufacture thereof |
| JPS60143762A (en) * | 1983-10-03 | 1985-07-30 | Mitsui Toatsu Chem Inc | Hydroxy apatite for chromatography |
| JPS60198458A (en) * | 1984-03-22 | 1985-10-07 | Koken:Kk | Column for chromatography |
-
1986
- 1986-03-31 JP JP61073946A patent/JPS62231163A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56151352A (en) * | 1980-04-26 | 1981-11-24 | Asahi Chem Ind Co Ltd | Column for gel permeation chromatography and manufacture thereof |
| JPS60143762A (en) * | 1983-10-03 | 1985-07-30 | Mitsui Toatsu Chem Inc | Hydroxy apatite for chromatography |
| JPS60198458A (en) * | 1984-03-22 | 1985-10-07 | Koken:Kk | Column for chromatography |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019187585A1 (en) * | 2018-03-30 | 2019-10-03 | 株式会社カネカ | Production method for packed bed that comprises polysaccharide column carrier packed into large column by pulsation suppression |
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