JPS62198731A - Method and device for sampling - Google Patents

Abstract

PURPOSE:To prevent a hydrophilic film from being clogged and to enable the hydrophilic film to be used for a long period by bringing sample liquid into contact with one surface of the hydrophilic film and carrier liquid into contact with the other surface. CONSTITUTION:When the carrier liquid is sent from an intake pipe 3 to an enzyme electrode 16 by pressure, it is discharged from an outlet pipe 4 through the pipe 3, the longitudinal hole 12 of a sampling part 1, a flow passage 8 contacting the hydrophilic film 9, a mixer 13, the longitudinal hole 21 of an electrode cell 2, a measurement chamber 22, and the lateral hole 23 of the cell 2. The carrier liquid is fed and stopped periodically and repeatedly through this path, a specific material in the sample liquid which penetrates the film 9 during stop periods is diffused in the carrier liquid, mixed in the mixing chamber 13 during pressure feed periods, and sent to the measurement chamber 22. At the same time, part of the carrier liquid is formed out of the film 9 to the sample liquid side to back-wash the film 9, which is therefore prevented from being clogged and usable for a long period.

Description

[Detailed Description of the Invention] Background of the Invention The invention is for the purpose of continuously or batchwise measuring a specific component at a high concentration beyond the measurement concentration range of a test liquid containing proteins or solids or a measuring device. pertains to sampling methods and devices.

BACKGROUND ART When measuring the concentration of a specific component in a test liquid such as blood, culture solution, or food, it is often necessary to remove proteins and solids from the test liquid before measurement.

Therefore, methods such as centrifugal separation, f-filtration, and dialysis are used as pretreatment. These pretreatments are generally used for batch measurements, but it is necessary to transport or transport the treated sample to the measuring device by some means before it can be submerged for measurement, which adds to the complexity of the operation. This causes a delay in measurement. If we tried to perform these operations automatically, the equipment would have to become complicated. Furthermore, when performing slow hardness measurements, there are a number of restrictions on continuously performing these pretreatments.

For example, if centrifugation is carried out continuously, a large amount of sample liquid is required, and in e-filtration or dialysis, clogging of the material and the dialysis membrane occurs. A periodic backwashing mechanism is effective in preventing clogging, but it is generally complicated when implemented as a device.

Also, if the measurement concentration range of 4M direct measurement is low, dilution operation may be necessary. For example, when measuring grape nitrate in blood 1 food or culture solution, an enzyme 1 electrode with a 9-structure is used, with the hydrogen peroxide electrode or the armpit being tightly crushed with the #element 1t4ii immobilized on the detection part. The concentration of Grape 81 that can be continuously measured with an electrode is 200 ppm or less (1), and the test liquid generally has a concentration more than 10 times this concentration, so a dilution mechanism for each tank has been devised.

For example, in a batch measuring device for blood glue, a fixed amount of diluted liquid is taken into a measuring container, and 1i-several microliters of serum is injected into it to measure the production rate of hydrogen peroxide and the rate of decrease of oxygen on the immobilized oxygen layer. is commonly used, and it is difficult to perform continuous measurements using this method. There is a method to analyze dialysate as a continuous blood sugar analysis method, but the dialysis membrane is not visible.
The ninth problem is that the dialysis volume is unstable, making it difficult to measure it reliably.

DISCLOSURE OF THE INVENTION The present invention overcomes these drawbacks and proposes a complete method and apparatus for sampling specific components from test fluids containing proteins and solids such as blood, culture fluid, and food. A carrier liquid is sent to a flow path formed on the opposite side of the surface in contact with the test liquid of a hydrophilic membrane that can completely permeate specific components dissolved in the liquid, while periodically changing the speed. shall be.

That is, one aspect of the present invention is a method for selectively kimpling a specific component ##L''C in a test liquid.

One side of the hydrophilic membrane, which is permeable to the specific component, is brought into contact with the test liquid, while the other side of the hydrophilic membrane is brought into contact with the flowing carrier liquid, so that the time-limiting component passes through the test liquid. A sampling method is provided in which a sample is transferred from a liquid through the hydrophilic membrane into the carrier liquid, and the carrier liquid is fed at a rate that is changed periodically, and furthermore, a desired specific component is A hydrophilic membrane that is to be in contact with the test liquid in which a 8! The present invention also provides a sampling device comprising a pump for pumping a carrier liquid under pressure, and a control device for periodically changing the speed at which the carrier liquid is pumped by the pump, either automatically or manually.

An effective embodiment of the present invention is to send the carrier liquid to a long and narrow channel formed in contact with a hydrophilic membrane, and repeat pressure feeding and stopping of the carrier liquid from upstream. This can be achieved by closing the upstream and downstream flow channels during the Gearia liquid stop time to keep the carrier liquid in contact with the hydrophilic membrane stationary and allowing the specific components in the test liquid to diffuse into the carrier liquid. For example, if the feeding of the carrier liquid is stopped periodically, specific substances in the test liquid may pass through the hydrophilic membrane while the carrier liquid is stopped.
When it penetrates into the carrier liquid and sends the liquid, it becomes constant.
The carrier liquid in the t-containing fLwI is forced out. If this is supplied to t-measurement #tl, a specific object i*rRt- can be measured. When the carrier liquid is pumped into the T-channel, a part of the carrier liquid is pushed out from the hydrophilic membrane surface and backwashes M, so the membrane is not clogged and can be used for a long time. Alternatively, it may be formed into a tube shape and the tube may be used as a flow path. Since the area of a specific W4 substance being dialyzed into a carrier liquid is proportional to the area of the hydrophilic membrane facing the flow path, it is best to choose between a flat membrane shape and a tube shape depending on the measurement concentration range of the measuring device. good.

Examples Next, the present invention is illustrated in the drawings, and according to nine examples, r', Pa
Explain.

First, in the embodiment shown in FIGS. 1-4.

Electric cell 2, carrier liquid filled vibrator 5, outlet vibrator 4,
The electrode guide 5 is integrally formed with the sampling part 1 and the bag pad 6, and a channel 8 through which the carrier liquid flows is formed at the tip of the O sampling part 1, which is fixed via a silicone rubber plate 7t. The hydrophilic 41A9 is sandwiched and fixed between the Q ring 10 and the Wara gloss 11, and a VB layer is formed on the spherical tip. Here are two enzyme electrodes, 16t, and a bag nut L1 through the washer and seven lie.
to fix the electrode 16t. The electrode 16 is constructed by fixing an immobilized #element film 18 on a detection part for hydrogen peroxide polarization and fixing it with a coring ring 20 through a nylon net 19i. When the carrier liquid is pumped to this electrode from the carrier liquid-filled tube 5, the pipe 5. Vertical hole 12 in the sampling section, flow path 8 in contact with hydrophilic membrane 9. Mixer 15. &IE is discharged from the outlet vibrator 4 through the vertical hole 21° of the other cell, the measurement chamber 22, and the horizontal hole 23 of the electric wound cell.

The mixing port 13 in this path is formed of glass wool 14 and glass beads 15. When a part of the buffer solution 7 is pumped as a carrier liquid into this path and stopped periodically, a specific substance in the test liquid, such as glucose, which has passed through the hydrophilic membrane 9 during the stop period, diffuses into the carrier liquid and is pumped. During this period, the carrier liquid is mixed in the mixer 15 and sent to the measurement chamber 22, and at the same time, a part of the carrier liquid is pushed out from the wood-philic membrane 9 to the test liquid side, and the hydrophilic membrane is backwashed.

The period for stopping and pumping the medium carrier liquid may be selected uniformly so that the concentration of the specific substance in the carrier liquid sent to the measuring device falls within the measurement range of the #element electrode.

To steam sterilize the non-electrode, remove the enzyme electrode 16, attach it to the main electrode T-failure test tank, steam sterilize it, insert the enzyme electrode, and hold it in the washer 241 with the bag nut 25.

Figure 5 shows another embodiment of the invention. Entrance vibe 5
The carrier liquid sent from the outer cylinder 26 and the inner cylinder 27 is the barrier 1!
It passes through J and reaches the inside of the thickener provided at the tip of the mixer 13t-
After passing through, the enzyme reaches the other 16 detection parts and 1 between ta and the cylinder! Jt4 is discharged from the exit vibrator 4. This embodiment is characterized in that it is easy to manufacture, since most of the parts other than the exit vibrator and the input vibrator can be formed by lathe processing. In this sensor as well, the same effect as in the previous embodiment can be obtained by periodically changing the liquid feeding rate.

It is convenient to change the liquid feeding speed by periodically stopping the pumping, but it is also possible to change the pumping speed and periodically repeat the pumping and suction by changing the pumping speed. In this case, is it necessary to make the pumping amount larger than the suction amount?

FIG. 5 shows a system diagram of a 5AM example of the present invention.

The carrier liquid 36 is sent from the inlet vibrator 5 to the tube 9 formed of the hydrophilic membrane 9 by the peristaltic pump 57, and is fed into the measuring instrument via the outlet vibrator 4t. When the peristaltic pump 27 is stopped and the pinch valve 28 is closed at the same time, the carrier liquid in the tube 9 becomes still. A specific component dissolved in the test liquid or diffused into the carrier liquid is used here.
The movement of the liquid inside and outside the tube occurs due to the hydrostatic pressure between the outlet of the tube and the liquid surface of the test liquid, making the diffusion rate of the specific substance unstable.As shown in this example, only the sampling device is attached to the test liquid. Measuring device downstream! It may be provided at f1.

[Brief explanation of drawings]

FIG. 1 is a sectional view of one embodiment of the present invention. FIG. 2 is a front end view of the sampling section 1 shown in FIG. 1, and shows a cross section of nine embodiments of the apparatus to which the present invention is applied. Figure 9, 746 shows an embodiment of the method of the present invention in a flow system. In the figure: Carrier liquid flow path 8, wood-philic membrane? , carrier liquid container 6
．． This is the pump 27.

Claims (1)

[Claims] 1. A method for selectively sampling a specific component dissolved in a test liquid, which comprises bringing one side of a hydrophilic membrane that is permeable to the specific component into contact with the test liquid. , while the other side of the hydrophilic membrane is brought into contact with a flowing carrier liquid, so that the specific component passes through the hydrophilic membrane from the test liquid and moves into the carrier liquid, and A sampling method characterized by feeding the liquid at periodically different speeds. 2. The sampling method according to claim 1, wherein the carrier liquid is repeatedly fed under pressure into the flow path from upstream and stopped. 3. During the suspension time of the carrier liquid, the upstream and downstream channels are closed to keep the carrier liquid in contact with the hydrophilic membrane stationary and diffuse the specific component in the test liquid into the carrier liquid. Sampling method described in Section 2. 4. A hydrophilic membrane to be in contact with a test liquid in which a specific component is dissolved, and a channel through which a carrier liquid formed by contacting the hydrophilic membrane surface on the opposite side to the test liquid contact surface flows;
1. A sampling device comprising: a pump that pumps a carrier liquid into a flow path; and a control device that periodically changes automatically or manually the speed at which the carrier liquid is pumped by the pump.