JPS62179385A - 豚パルボウイルスの弱毒株の作出法 - Google Patents
豚パルボウイルスの弱毒株の作出法Info
- Publication number
- JPS62179385A JPS62179385A JP1922586A JP1922586A JPS62179385A JP S62179385 A JPS62179385 A JP S62179385A JP 1922586 A JP1922586 A JP 1922586A JP 1922586 A JP1922586 A JP 1922586A JP S62179385 A JPS62179385 A JP S62179385A
- Authority
- JP
- Japan
- Prior art keywords
- virus
- strain
- cells
- cell
- skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000282898 Sus scrofa Species 0.000 title claims abstract description 27
- 241000125945 Protoparvovirus Species 0.000 title claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 title claims description 13
- 231100000331 toxic Toxicity 0.000 title abstract 3
- 230000002588 toxic effect Effects 0.000 title abstract 3
- 241000283690 Bos taurus Species 0.000 claims abstract description 25
- 230000002238 attenuated effect Effects 0.000 claims description 19
- 210000004927 skin cell Anatomy 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 76
- 241000700605 Viruses Species 0.000 abstract description 71
- 102000004142 Trypsin Human genes 0.000 abstract description 5
- 108090000631 Trypsin Proteins 0.000 abstract description 5
- 239000012588 trypsin Substances 0.000 abstract description 5
- 230000001605 fetal effect Effects 0.000 abstract description 4
- 244000309466 calf Species 0.000 abstract description 3
- 210000003754 fetus Anatomy 0.000 abstract description 3
- 230000007918 pathogenicity Effects 0.000 abstract description 3
- 210000002966 serum Anatomy 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000036039 immunity Effects 0.000 abstract 1
- 210000005229 liver cell Anatomy 0.000 abstract 1
- 241000702619 Porcine parvovirus Species 0.000 description 42
- 241000282887 Suidae Species 0.000 description 27
- 210000003491 skin Anatomy 0.000 description 21
- 210000004748 cultured cell Anatomy 0.000 description 19
- 238000012360 testing method Methods 0.000 description 12
- 229960005486 vaccine Drugs 0.000 description 12
- 230000012010 growth Effects 0.000 description 10
- 239000006285 cell suspension Substances 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 8
- 208000017520 skin disease Diseases 0.000 description 8
- 230000005727 virus proliferation Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 230000010261 cell growth Effects 0.000 description 6
- 239000013256 coordination polymer Substances 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 208000015181 infectious disease Diseases 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000002255 vaccination Methods 0.000 description 5
- 206010058874 Viraemia Diseases 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 230000007850 degeneration Effects 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 208000008071 Parvoviridae Infections Diseases 0.000 description 3
- 230000005856 abnormality Effects 0.000 description 3
- 230000029142 excretion Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 241000710777 Classical swine fever virus Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 206010057343 Parvovirus infection Diseases 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000000120 cytopathologic effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 239000003760 tallow Substances 0.000 description 2
- FXKZPKBFTQUJBA-UHFFFAOYSA-N 2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;sodium;dihydrate Chemical compound O.O.[Na].[Na].OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O FXKZPKBFTQUJBA-UHFFFAOYSA-N 0.000 description 1
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- 101100488074 Bacillus subtilis (strain 168) yclD gene Proteins 0.000 description 1
- 206010011732 Cyst Diseases 0.000 description 1
- 241000257465 Echinoidea Species 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000002079 cooperative effect Effects 0.000 description 1
- 208000031513 cyst Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000008235 industrial water Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- OOYGSFOGFJDDHP-KMCOLRRFSA-N kanamycin A sulfate Chemical group OS(O)(=O)=O.O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N OOYGSFOGFJDDHP-KMCOLRRFSA-N 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 208000015994 miscarriage Diseases 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000009366 sericulture Methods 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 208000002254 stillbirth Diseases 0.000 description 1
- 231100000537 stillbirth Toxicity 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1922586A JPS62179385A (ja) | 1986-01-31 | 1986-01-31 | 豚パルボウイルスの弱毒株の作出法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1922586A JPS62179385A (ja) | 1986-01-31 | 1986-01-31 | 豚パルボウイルスの弱毒株の作出法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62179385A true JPS62179385A (ja) | 1987-08-06 |
| JPH0582192B2 JPH0582192B2 (enrdf_load_stackoverflow) | 1993-11-17 |
Family
ID=11993429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1922586A Granted JPS62179385A (ja) | 1986-01-31 | 1986-01-31 | 豚パルボウイルスの弱毒株の作出法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62179385A (enrdf_load_stackoverflow) |
-
1986
- 1986-01-31 JP JP1922586A patent/JPS62179385A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0582192B2 (enrdf_load_stackoverflow) | 1993-11-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |