JPS62148647A - Blood sampling tube having blood coagulating action - Google Patents
Blood sampling tube having blood coagulating actionInfo
- Publication number
- JPS62148647A JPS62148647A JP60291339A JP29133985A JPS62148647A JP S62148647 A JPS62148647 A JP S62148647A JP 60291339 A JP60291339 A JP 60291339A JP 29133985 A JP29133985 A JP 29133985A JP S62148647 A JPS62148647 A JP S62148647A
- Authority
- JP
- Japan
- Prior art keywords
- blood
- coagulation
- collection tube
- blood collection
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
■１発明の背景
［技術分野］
この発明は、採血管に係り、特には血液凝固作用を有す
る採血管に関する。Detailed Description of the Invention (1) Background of the Invention [Technical Field] The present invention relates to a blood collection tube, and particularly to a blood collection tube having a blood coagulation effect.
［先行技術および問題点コ
現在、各種臨床検査のために、患者から採血管に採取し
た血液から血清を分離してこれを検査に供することかお
こなわれている。血液から血清を分離するためには、血
清以外の血液成分を凝固させて血餅とした後、採血層を
速心分離する。したかって、患者から血液を採取する際
に用いられる採血管には予め血液凝固促進剤を加えてお
くことか望ましい。[Prior Art and Problems] Currently, for various clinical tests, serum is separated from blood collected from a patient into a blood collection tube and the serum is used for testing. In order to separate serum from blood, blood components other than serum are coagulated to form a blood clot, and then the blood sample layer is subjected to centrifugal separation. Therefore, it is desirable to add a blood coagulation promoter in advance to the blood collection tube used to collect blood from a patient.
米国特許第４１５３７３９号明細書に、採血管に血液凝
固促進剤を予め加える手段として、ケイ酸質の血液凝固
促進剤を水溶性高分子を媒体として採血管内面に塗布す
る方法が記載されている。U.S. Pat. No. 4,153,739 describes a method of applying a silicic acid blood coagulation promoter to the inner surface of the blood collection tube using a water-soluble polymer as a medium, as a means of adding the blood coagulation promoter to the blood collection tube in advance. .
しかしながら、この手法によると、凝固促進剤の量にバ
ラツキが生じやすく凝固が不充分であったり溶血などの
現象を生じたりしていた。However, according to this method, the amount of coagulation promoter tends to vary, leading to insufficient coagulation or phenomena such as hemolysis.
■１発明の目的
したがって、この発明の１」的は、採血後比較的速やか
に血液凝固作用を発揮する採血管を提供することにある
。(1) Aim of the Invention Accordingly, an object of the present invention is to provide a blood collection tube that exerts a blood coagulation effect relatively quickly after blood collection.
この発明によれば、採血管内に、水溶性高分子中に血液
凝固促進剤が分散されてなる凝固促進性材料か多孔質塊
状形態で設置されていることを特徴とする採血管か提供
される。According to the present invention, there is provided a blood collection tube characterized in that a procoagulant material comprising a blood coagulation promoter dispersed in a water-soluble polymer is installed in the blood collection tube in the form of a porous block. .
」二記凝固促進性＋４料は、凍結乾燥されていることか
好ましい。It is preferable that the coagulation-promoting agent described in item 2 above (4) is freeze-dried.
■２発明の具体的構成
以下、図面を参照してこの発明の詳細な説明する。全図
中同一部分は同一７１号で示す。(2) Specific structure of the invention The present invention will be described in detail below with reference to the drawings. Identical parts in all figures are designated by the same number 71.
第１図には、この発明の第１の態様に従う採血盾か示さ
れている。この採血管１０は、採取した血液を収容する
ための管１１を有し、その底部に凝固促進性材料１２か
塊状形態で載置されている。FIG. 1 shows a blood collection shield according to a first aspect of the invention. This blood collection tube 10 has a tube 11 for containing collected blood, and a procoagulant material 12 is placed in the form of a lump at the bottom of the tube 11 .
管１１は、ガラスまたはプラスチックで形成されていダ
る。The tube 11 is made of glass or plastic.
凝固促進性材料１２は、水溶性高分子中に血液凝固促進
剤を分散させるものからなり、多孔質の形態を釘してい
る。水溶性高分子としては、＋ｆｕ ｉ＆と接触して直
ちに溶解するものであれは特に制限はないか、後の検査
に対して影響が少ないポリビニルピロリドンが好ましい
。The procoagulant material 12 consists of a blood coagulation promoter dispersed in a water-soluble polymer and has a porous form. There are no particular restrictions on the water-soluble polymer as long as it dissolves immediately upon contact with +fu i&, or polyvinylpyrrolidone, which has little effect on later tests, is preferred.
＋ｆｕ ’ｔ＆凝固促進剤としては、シリカ、滑石、ガ
ラス、ゼオライト、チタンホワイト、マイカ等の無機粉
末、オレイン酸などが使用できる。しかしながら、凝固
促進性に優れていることから、ケイ素系無機粉末（シリ
カ、滑石、ガラス、ゼオライト）、およびチタンホワイ
トか好ましい。As the +fu 't & coagulation accelerator, inorganic powders such as silica, talc, glass, zeolite, titanium white, mica, etc., oleic acid, etc. can be used. However, silicon-based inorganic powders (silica, talc, glass, zeolite) and titanium white are preferred because they have excellent coagulation promoting properties.
凝固促進性材料１２中に占める血液凝固促進剤の割合は
２０ないし８０ｉＲ瓜％であることか好ましい。その割
合か２０重量％未満であると、血液凝固促進性が充分て
なく、また血液中に溶解する水溶性高分子の量が多くな
らで、後の血液検査に悪影響を及ぼすおそれがある。他
方、その割合か８０重量％を越えると、血液中の分散性
が低下し、血液に対する凝固促進性が充分に発揮されな
い。Preferably, the proportion of the blood coagulation promoter in the procoagulant material 12 is 20 to 80 iR%. If the proportion is less than 20% by weight, blood coagulation promoting properties will not be sufficient and the amount of water-soluble polymer dissolved in the blood will be large, which may adversely affect subsequent blood tests. On the other hand, if the proportion exceeds 80% by weight, the dispersibility in blood decreases and the coagulation-promoting property for blood is not sufficiently exhibited.
Ｕ固促進性材料１２の車量は採取した血液１０ミリリッ
トル当り、５〜１００６であることか好ましい。５ｆｌ
ｔｇ未満であると、血液凝固効果か少なく、他方１００
ｎを越えると、溶血現象か生じるおそれかある。It is preferable that the amount of U solidification promoting material 12 is 5 to 1006 per 10 ml of collected blood. 5fl
If it is less than tg, the blood coagulation effect will be less, and on the other hand, 100
If it exceeds n, hemolysis may occur.
既述のように、凝固促進性材料１２は多孔質の塊状形態
にある。このような多孔質塊を得るためには、」−記高
分子を水に溶解し、この水溶１ｆＪ２に血液凝固促進剤
を、例えば超音波分散機を用いて充分に分散させて分散
液を作る。この分散液をアセトン基の凝固浴に浸漬して
多孔質膜、繊維とすることができる。しかしながら、最
も適切な方法は、上記分散液を凍結乾燥する方法である
。この方法によって得られた凝固促進材料１２は綿状多
孔質塊状形態であり、血液中に速やかに溶解して血液Ｕ
固促進剤を分散させることができる。As previously mentioned, the pro-coagulant material 12 is in porous bulk form. In order to obtain such a porous mass, the above-mentioned polymer is dissolved in water, and a blood coagulation promoter is sufficiently dispersed in this water-soluble 1fJ2 using, for example, an ultrasonic dispersion machine to prepare a dispersion. . This dispersion can be immersed in an acetone-based coagulation bath to form a porous membrane or fiber. However, the most suitable method is to lyophilize the dispersion. The coagulation-promoting material 12 obtained by this method is in the form of a flocculent porous mass, and quickly dissolves in the blood.
A solidification promoter can be dispersed.
第２図は、この発明の第２の態様に従う血清分離剤入り
減圧採血管である。この減圧採血管２０は、管１１内が
減圧されていて、口部が閉塞部材２１で閉塞され、減圧
状態が保持されている。管１１内部には、血清分離剤２
２が収容され、その」二に凝固促進性材料１２が載置さ
れている。血清分離剤２２は、血液凝固促進剤で凝固形
成された血餅と血清との中間の比重を有し、後の遠心分
離により、血餅と血清との間に位置するものである。FIG. 2 is a vacuum blood collection tube containing a serum separating agent according to a second embodiment of the present invention. This reduced pressure blood collection tube 20 has a reduced pressure inside the tube 11, and its mouth is closed with a closing member 21 to maintain the reduced pressure state. Inside the tube 11, there is a serum separating agent 2.
2 is housed therein, and a coagulation accelerating material 12 is placed on the second. The serum separating agent 22 has a specific gravity between that of a blood clot formed by a blood coagulation promoter and serum, and is located between the blood clot and the serum by subsequent centrifugation.
管１１内の減圧度は、採取すべき所定の血液計を吸引で
きる程度である。The degree of vacuum in the tube 11 is such that a predetermined blood sample to be collected can be aspirated.
■９発明の具体的作用および効果
この発明の採血管に血液を採取すると、多孔質塊状であ
る凝固促進性材料は、そのマトリフクスを（１η成する
水溶性高分子か速やかに血ｉｎｋ中に溶解する結果、血
液凝固促進剤の全量が直ちに血液中に分散しその血液凝
固作用が発揮される。したかって、血液の凝固が迅速に
進行し、また血液に対する促進剤の量も充分となるので
、凝固不良（溶血）やフィブリンの析出などの問題か解
消される。■9 Specific Actions and Effects of the Invention When blood is collected into the blood collection tube of this invention, the procoagulant material, which is a porous mass, quickly dissolves into the blood ink as a water-soluble polymer forming a matrix of (1η). As a result, the entire amount of the blood coagulation promoter is immediately dispersed into the blood and its blood coagulation effect is exerted.Therefore, blood coagulation progresses quickly and the amount of the promoter in the blood is sufficient. Problems such as poor coagulation (hemolysis) and fibrin precipitation are resolved.
［実施例］
水溶性高分子としてポリビニルピロリドン（東京化成社
製ＰＶＰ Ｋ ９０） 、血液凝固促進剤としてケイ素
系無機粉末（ペンシルバニア・グラス・サンド社製旧ｎ
−Ｕ−８ｊ１ ）またはチタンホワイト（富士チタン工
業社製１’Ｒ８４０）とを表１に示すはで／ｋ １００
ｇに溶解・分散させた。その際、１ｆｌｔ １（Ｊｉ
凝固促進剤を充分に分散させるために、超音波分散機を
用いた。こうして得た分散ｉｔｋを凍結乾燥し、多孔質
塊状形態の凝固促進性材料を得た。表１に示す各種試験
官（内容積１３ｅｅ）に同表に記載されている瓜の凝固
促進性材料を入れ、直ちに、シリンジにより採取したヒ
ト血液を１０ｅｅ注入した。[Example] Polyvinylpyrrolidone (PVP K 90 manufactured by Tokyo Kasei Co., Ltd.) was used as a water-soluble polymer, and silicon-based inorganic powder (formerly N manufactured by Pennsylvania Glass Sands Co., Ltd.) was used as a blood coagulation promoter.
-U-8j1) or titanium white (1'R840 manufactured by Fuji Titanium Industries Co., Ltd.) as shown in Table 1/k 100
It was dissolved and dispersed in g. At that time, 1flt 1 (Ji
An ultrasonic disperser was used to sufficiently disperse the coagulation accelerator. The thus obtained dispersed itk was freeze-dried to obtain a coagulation-promoting material in the form of a porous mass. The melon coagulation-promoting material listed in Table 1 was placed in each tester (inner volume 13ee) shown in Table 1, and immediately 10ee of human blood collected with a syringe was injected.
血液の凝固時間、血清分離状態等を、凋へた。なお、遠
心は採血３０分後におこなった。結果を表１に示す。Blood coagulation time, serum separation status, etc. decreased. Note that centrifugation was performed 30 minutes after blood collection. The results are shown in Table 1.
表１から明らかなように、本発明品である実施例におい
ては、凝固が迅速に進行し、凝固不良（溶血）やフィブ
リン析出などの問題か生じなかったが、比較例において
は、凝固時間が長く、耐固不良（溶血）や、フィブリン
析出が認められた。As is clear from Table 1, coagulation progressed quickly in the Example, which is a product of the present invention, and no problems such as poor coagulation (hemolysis) or fibrin precipitation occurred, but in the Comparative Example, the coagulation time was For a long time, poor solidification resistance (hemolysis) and fibrin precipitation were observed.
第１図および第２図はそれぞれこの発明に従う採血管を
示す断面図。
１１・・・管、１２・・・凝固促進性材料、２１・・・
閉塞部材、２２・・・血ｌ＾分離剤。
出願人代理人 弁理士 鈴江武彦
第１図 第２図FIG. 1 and FIG. 2 are sectional views each showing a blood collection tube according to the present invention. 11... Tube, 12... Coagulation promoting material, 21...
Closure member, 22...Blood l^ separation agent. Applicant's agent Patent attorney Takehiko Suzue Figure 1 Figure 2
Claims (2)
分散されてなる凝固促進性材料が多孔質塊状形態で設置
されていることを特徴とする採血管。(1) A blood collection tube characterized in that a procoagulant material made of a water-soluble polymer and a blood coagulation promoter dispersed in a porous mass is installed in the blood collection tube.
範囲第１項記載の採血管。(2) The blood collection tube according to claim 1, wherein the procoagulant material is in a freeze-dried state.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60291339A JPS62148647A (en) | 1985-12-24 | 1985-12-24 | Blood sampling tube having blood coagulating action |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60291339A JPS62148647A (en) | 1985-12-24 | 1985-12-24 | Blood sampling tube having blood coagulating action |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS62148647A true JPS62148647A (en) | 1987-07-02 |
Family
ID=17767638
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60291339A Pending JPS62148647A (en) | 1985-12-24 | 1985-12-24 | Blood sampling tube having blood coagulating action |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62148647A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08299309A (en) * | 1995-04-28 | 1996-11-19 | Becton Dickinson & Co | Blood-gathering assembly containing glass insertion member for coagulation promotion |
| JP2010222376A (en) * | 2003-05-21 | 2010-10-07 | Jms Co Ltd | Serum preparation apparatus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5827933A (en) * | 1981-08-13 | 1983-02-18 | Kawasaki Steel Corp | Production of t-3 mild blackplate having excellent corrosion resistance by continuous annealing |
| JPS5939129A (en) * | 1982-08-27 | 1984-03-03 | Aisin Seiki Co Ltd | On-vehicle telephone set |
-
1985
- 1985-12-24 JP JP60291339A patent/JPS62148647A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5827933A (en) * | 1981-08-13 | 1983-02-18 | Kawasaki Steel Corp | Production of t-3 mild blackplate having excellent corrosion resistance by continuous annealing |
| JPS5939129A (en) * | 1982-08-27 | 1984-03-03 | Aisin Seiki Co Ltd | On-vehicle telephone set |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08299309A (en) * | 1995-04-28 | 1996-11-19 | Becton Dickinson & Co | Blood-gathering assembly containing glass insertion member for coagulation promotion |
| JP2010222376A (en) * | 2003-05-21 | 2010-10-07 | Jms Co Ltd | Serum preparation apparatus |
| US8993321B2 (en) | 2003-05-21 | 2015-03-31 | Jms Co., Ltd. | Container for preparing serum and regenerative medical process using the same |
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