JPS62145016A - Antibacterial agent - Google Patents

Abstract

PURPOSE:To provide an antibacterial agent containing a specific flavonoid compound as an active component and exhibiting suppressing effect against indigenous dermatic bacteria. CONSTITUTION:A flavonoid compound selected from 5,7-dihydroxy-4'- methoxyisoflavone, 3'4',5,7-tetrahydroxyflavanone and l-epigallocatechin is used as an active component of the objective antibacterial agent at an amount of 0.01-1wt% of the whole composition. The compound is used together with proper base, diluent, etc. It is effective against Staphylococcus epidermis which is an indigenous dermatic bacterium. The agent is useful for the prevention and remedy of dermatic diseases such as acne vulgaris (pimple). The base and additive are e.g. oil, water, surfactant, alcohol, chelating agent, antioxidant, thickener, pigment, antiseptic, etc., and the agent is used in the form of solution, emulsion, powder, dispersion, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to an antibacterial agent. Specifically, the present invention relates to an antibacterial agent that exhibits an inhibitory effect on resident skin bacteria.

(Structure of the Invention) As a result of studying the pharmacological activities of physiologically active substances contained in various plants, the present inventors found that certain flavonoid compounds are associated with Staphylococcus aevidermides, a resident skin bacteria. ■The present invention was achieved by confirming that it exhibits excellent antibacterial activity against HBJ.

To explain the present invention in detail, the flavonoid compounds that are the active ingredients of the present invention include 5
．． 7-cyhydroxy-4-methoxyisoflavone (Bi
ochanin A, compound l), go, 4', 5
, 7-thitrahydroxyflavanone (Er1di
octyol, compound 2), or l-x bigalocatechin (J'-Epigal Iocatechin, compound 3).

These compounds are, for example, 8iochelIIical
Pharaa-cology, Volume 32, No. 7, 1141
~page 114B (1983) or Tetrached
Ron, Vol. 29, p. 125 (1973), etc., and these compounds exist in plants. It will be done.

The above-mentioned flavonoid compounds do not need to be isolated and pure products, but extracts, partially purified products, etc. containing them are used, and usually these active ingredients are mixed with appropriate bases, diluents, etc. in total amount. Medium 0.01~! It is used in such a way that it contains about % by weight.

(Effect of the invention) These flavone compounds are as shown in the examples below:
Shows effective antibacterial activity against Staphylococcus evidermides, a resident bacteria on the skin. Therefore, these compounds are effective against direct stimulation of these bacteria, such as Propionibacterium acnes, and stimulation of free fatty acids produced by the lipase action of these bacteria. An example of a skin disease in which both effects are thought to be involved is acne vulgaris.
Vulgaris) is useful for prevention, treatment, etc.

When using the antibacterial agent of the present invention, oil, water, surfactant, alcohol, chelating agent, antioxidant, thickener, pigment, preservative, etc. are used as a base and additives, and in the form of a solution, It is used in forms such as emulsion, powder, and dispersion.

(Example,) The present invention will be described in more detail below with reference to Examples.

[Antibacterial test] Agar plate dilution method shown below.
The anti-seedling activity of this substance was measured using the onmethod.

That is, a sample compound α1m1 was suspended in a 5% dimethyl sulfoxide aqueous solution to a predetermined concentration, and a sample reservoir α1m1 was placed in a sterile petri dish (9 cm x 2 cm), and a culture medium for susceptible discs that had been previously sterilized at 120°C for 15 minutes was placed there. (manufactured by Eiken Chemical Co., Ltd.) 9 ml was added, thoroughly mixed, and solidified on a flat plate.

Test bacteria prepared in advance using a platinum loop with an inner diameter of 1 mm were spread and inoculated onto the above-mentioned flat plate to a length of about 2 cm, and cultured at 30°C for 18 to 20 hours. The old C value (mi old mu
m 1nhibitory concentration
n).

The test bacteria were cultured at 30° C. for 18 to 20 hours using heart infusion broth medium (manufactured by Eiken Kagaku Co., Ltd.) and diluted 100 times with physiologically active saline immediately before use.

By the above test method, Staphylococcus epidermides (o e 'd rm'dis, ATCC
The results of measuring the old C value of each sample compound against 12228) are shown in Table 1 on the next page.

Table 1

Claims (1)

[Claims] (1) Skin resident bacteria whose active ingredients are flavonoid compounds selected from 5,7-dihydroxy-4'-methoxyisoflavone, 3',4',5,7-tetrahydroxyflavanone, and l-epigallocatechin. An antibacterial agent that shows an inhibitory effect on