JPS62135769A - 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 - Google Patents
細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬Info
- Publication number
- JPS62135769A JPS62135769A JP27611885A JP27611885A JPS62135769A JP S62135769 A JPS62135769 A JP S62135769A JP 27611885 A JP27611885 A JP 27611885A JP 27611885 A JP27611885 A JP 27611885A JP S62135769 A JPS62135769 A JP S62135769A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- fluorescence
- hematoporphyrin
- protein
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 229960003569 hematoporphyrin Drugs 0.000 claims abstract description 77
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- YBFCBQMICVOSRW-UHFFFAOYSA-N 1-phenylindole Chemical compound C1=CC2=CC=CC=C2N1C1=CC=CC=C1 YBFCBQMICVOSRW-UHFFFAOYSA-N 0.000 abstract 1
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 24
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 230000013777 protein digestion Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
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- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 201000009030 Carcinoma Diseases 0.000 description 4
- 238000010867 Hoechst staining Methods 0.000 description 4
- 206010036790 Productive cough Diseases 0.000 description 4
- 239000006285 cell suspension Substances 0.000 description 4
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- 208000002495 Uterine Neoplasms Diseases 0.000 description 3
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- 206010046766 uterine cancer Diseases 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 208000009956 adenocarcinoma Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
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- 208000020816 lung neoplasm Diseases 0.000 description 2
- 230000003211 malignant effect Effects 0.000 description 2
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- 238000012216 screening Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 206010041823 squamous cell carcinoma Diseases 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000000116 DAPI staining Methods 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
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- 208000037887 cell injury Diseases 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
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- 238000001816 cooling Methods 0.000 description 1
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- 230000000120 cytopathologic effect Effects 0.000 description 1
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- 102000038379 digestive enzymes Human genes 0.000 description 1
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- 210000000440 neutrophil Anatomy 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
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Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27611885A JPS62135769A (ja) | 1985-12-10 | 1985-12-10 | 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP27611885A JPS62135769A (ja) | 1985-12-10 | 1985-12-10 | 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62135769A true JPS62135769A (ja) | 1987-06-18 |
| JPH0577028B2 JPH0577028B2 (enExample) | 1993-10-25 |
Family
ID=17565043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP27611885A Granted JPS62135769A (ja) | 1985-12-10 | 1985-12-10 | 細胞中の蛋白質とｄｎａの比率の測定法及び測定用試薬 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62135769A (enExample) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01242962A (ja) * | 1988-03-24 | 1989-09-27 | Agency Of Ind Science & Technol | 植物細胞核を標識する方法および装置 |
| JPH02168161A (ja) * | 1988-12-22 | 1990-06-28 | Nikon Corp | 細胞の良・悪性判定装置 |
| JPH03119991A (ja) * | 1989-10-03 | 1991-05-22 | Hamamatsu Photonics Kk | 細胞内遺伝情報消去方法およびその装置 |
| EP0812920A1 (en) * | 1996-06-14 | 1997-12-17 | Packard Instrument B.V. | Use of porphyrins in instrumental detection methods |
| US6001573A (en) * | 1996-06-14 | 1999-12-14 | Packard Bioscience B.V. | Use of porphyrins as a universal label |
-
1985
- 1985-12-10 JP JP27611885A patent/JPS62135769A/ja active Granted
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01242962A (ja) * | 1988-03-24 | 1989-09-27 | Agency Of Ind Science & Technol | 植物細胞核を標識する方法および装置 |
| JPH02168161A (ja) * | 1988-12-22 | 1990-06-28 | Nikon Corp | 細胞の良・悪性判定装置 |
| JPH03119991A (ja) * | 1989-10-03 | 1991-05-22 | Hamamatsu Photonics Kk | 細胞内遺伝情報消去方法およびその装置 |
| EP0812920A1 (en) * | 1996-06-14 | 1997-12-17 | Packard Instrument B.V. | Use of porphyrins in instrumental detection methods |
| US5998128A (en) * | 1996-06-14 | 1999-12-07 | Packard Instrument B.V. | Use of porphyrins in instrumental detection methods |
| US6001573A (en) * | 1996-06-14 | 1999-12-14 | Packard Bioscience B.V. | Use of porphyrins as a universal label |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0577028B2 (enExample) | 1993-10-25 |
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