JPS6211541A - Oil-in-water type emulsion - Google Patents

Abstract

PURPOSE:To increase the emulsion stability by emulsifying an oil phase component with the purified saponin obtained by on a synthetic high molecular adsorbent and deadsorbing this saponin with a hydrated hydrophilic organic solvent. CONSTITUTION:An extract contg. saponin is obtained by extracting a bark of Quillaja.saponalia or a pericarp of Sapindus mukurossi with water or a hydrated hydrophilic organic solvent. After treating this extract with a synthetic high molecular adsorbent, purified saponin is obtained by deadsorbing this saponin adsorbed on the high molecular adsorbent with the hydrated hydrophilic organic solvent. While stirring strongly the aq. soln. of the purified saponin, fat and oil are slowly added therein to emulsify the mixture and this is treated with a homogenizer or the like to prepare an oil-in-water type emulsion. As the above-mentioned hydrated hydrophilic organic solvent, ethanol and acetone, etc. are used and polyacrylic acid ester series are used as the synthetic high molecular adsorbent.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a highly stable oil-in-water emulsion.

Quillaia saponaria (to the strays)
ria MoL (Rosaceae) and the bark of Sapin (Sapin).
saponin contained in the pericarp of dus mukurossi GAERTNER + Sapindaceae) has been used as detergents, hair washes, and soap substitutes since early on by taking advantage of its foaming power.
It has been used to make emulsions for photographic materials and emulsions for sheet paper. In recent years, saponins have also come to be widely used as emulsifiers for foods, cosmetics, pharmaceuticals, etc., as it has been recognized that natural surfactants are more advantageous than synthetic substances in terms of safety.

However, the extract obtained by extracting the saponin-containing substances mentioned above with water or alcohol contains, in addition to saponins, proteins, sugars, amino acids, tannins, Kuroiso salts, etc.
If the extract containing many biological components is used as an emulsifier without sufficient purification, highly reactive tannins and Kuroiso salts will deteriorate the stability of the emulsion. For this reason, saponins used in emulsifiers are usually purified by some method. Conventional methods for purifying saponin-containing extracts obtained from Quillaja bark and Sapindica peel include repeated solvent precipitation using ether, precipitation from an alcohol solution, and treatment with activated carbon. These purification methods have not been able to sufficiently remove the impurities that impair emulsion stability. Therefore, even when purified saponin is used, the stability of emulsions containing saponin as an emulsifier is insufficient for many applications.

Problems to be Solved by the Invention The object of the present invention is to provide an oil-in-water emulsion of purified saponin 51 that does not have the above-mentioned drawbacks.

Means for Solving the Problems The oil-in-water emulsion of the present invention, which has succeeded in achieving the above objects, is obtained by extracting the bark of Quillaja saponaria or the pericarp of Sapindica with water or a hydrophilic organic solvent containing water. The purified saponin obtained by a new purification method involves treating a saponin-containing extract with a synthetic polymer adsorbent and desorbing the saponin adsorbed to the polymer adsorbent with a water-containing hydrophilic organic solvent. It is made by emulsification.

Details of the saponin purification method for the emulsion of the present invention are as follows. First, crushed saponin-containing bark or pericarp is extracted with water or a water-containing hydrophilic organic solvent (eg, ethanol, acetone, propyl alcohol, etc.) by a conventional method to obtain a saponin-containing extract. If the obtained extract contains an organic solvent, the solvent is distilled off, and then
Saponin is adsorbed by contacting with a synthetic polymer adsorbent (eg, styrene/divinylbenzene copolymer type, polyacrylic acid ester type, etc.). The method for bringing the extract into contact with the adsorbent may be either a batch method or a column method. The amount of adsorbent required to adsorb all of the saponins in the extract varies depending on the type of adsorbent, but usually 6-saponin is used in an amount of 0.5 to 10 times the weight of Quillaja bark or Sapindica peel as the raw material. After thoroughly washing the adsorbed adsorbent with water, when it is brought into contact with a water-containing hydrophilic organic solvent (for example, 30-80% weight ethanol),
As the saponin is desorbed, by collecting the desorption liquid, removing the solvent, and drying it, a light brown purified saponin can be obtained. In addition, some biological components other than saponin are adsorbed to the adsorbent, which is then worn on the skin through the above-mentioned underwear treatment. If the adsorbent is brought into contact with an alkaline aqueous solution before the desorption treatment, the saponin will not be desorbed and the above biological components other than saponin will be attached to the skin, so if saponin is attached after the alkaline aqueous solution treatment, higher purity can be obtained. You can obtain saponin with less coloring.

The method for producing the oil-in-water emulsion of the present invention using the purified saponin obtained as described above as an emulsifier is arbitrary, but usually, fats and oils are added to the aqueous solution of the purified saponin with strong stirring. It is desirable to gradually add the oil to emulsify it, and then further treat it with a homogenizer or the like to make the oil droplets fine and uniform.

The emulsifier in the oil-in-water emulsion of the present invention includes Quillajasaponin and Muclodisaponin alone or in a mixture, as well as other emulsifiers (for example, lecithin, sucrose fatty acid ester, glycerin fatty acid ester, taurocholic acid, tongue juice). (acids, etc.) may be used in combination. The emulsion of the present invention can also contain polysaccharides such as gum arabic, xanthan gum, pullulan, and proteins as stabilizers.

There is no particular restriction on the type of fats and oils contained in the oil-in-water emulsion of the present invention, including animal and vegetable oils such as pork tallow, beef tallow, soybean oil, olive oil, corn oil, beeswax, rice bran oil, and squalane, as well as fat-soluble fats and oils. It can be vitamins, various mineral oils, etc.

The oil-in-water emulsion of the present invention may contain any other substance in addition to the emulsified fat or oil, as long as the stability thereof is not seriously impaired. Therefore, the oil-in-water emulsion of the present invention can take the form of foods, cosmetics, pharmaceuticals, various oil and fat products, and raw materials thereof.

Action/Effect The refined saponin that the oil-in-water emulsion of the present invention contains as an emulsifier has a lower content of tannins and inorganic salts that deteriorate emulsion stability than saponins conventionally used in the production of this type of emulsion. It has the feature of low therefore,
The oil-in-water emulsion according to the present invention does not show separation of the oil layer even when stored for a long period of time, and has excellent heat resistance and acid resistance.

medicine phoenix The present invention will be described below with reference to Examples and Comparative Examples.

Example 1 10 e of water was added to 1 kg of Quillaja bark crushed material, heated to 70° C., and extracted with stirring for 2 hours. The extract was collected by decantation, and the same extraction procedure was repeated using 10e of water to obtain a total of 16e of extract. After filtering to remove solid matter, this extract is treated with a synthetic polymer adsorbent, Diaion H.
It was supplied to a column packed with P-20 (Mitsubishi Kasei) 2e at a space velocity of 1e/Ltlr. After this, change the column to 4Q's 0
．． The sample was washed with 3% ammonia water and then with water. Next, 60% ethanol 10e was added to the space velocity IQ/Q・Hr
The desorbed solution was concentrated to dryness under reduced pressure to obtain 92 g of light brown purified saponin. This purified saponin has a tannin content of 6.4%, an ash content of 2.8%, and a
The valence metal (Mg+Ca) content was 1,960 pp111.

Dissolve 1 g of the above purified saponin in 100 g of 20% liquid sugar, warm to 60°C, and while stirring with a homomixer at 4000 rpm, dropwise add 100 g of cottonseed salad oil at 60°C, and further stir for 2 minutes at 8000 rpm. and emulsified. The obtained emulsion maintained a stable emulsified state for more than one week when left at 30°C.

Comparative Example 1 3.30 ml of Quillaja bark extract obtained in the same manner as in Example 1 was concentrated to 200 ml, and 80 ml of ethanol was added to it.
0 and 1 were added to precipitate saponin. The precipitate was collected by filtration and dried under reduced pressure to obtain 13 g of brown powdered purified saponin. This purified saponin has a tannin content of 13.2%.
, ash content 10.8%, divalent metal content 19vllOppm
It belonged to

Using 1 g of the above purified saponin, an emulsion was prepared in the same manner as in Example 1 and left at 30°C.
The emulsification system was destroyed on the first day.

Example 2 Add 10e of 80% ethanol to 1kg of Sapindica peel,
Reflux extraction was performed for 2 hours. The extract was collected by decantation, and the same extraction operation was repeated using 10e of water to obtain a total of 18.5 g of extract. Ethanol was distilled off by heating under reduced pressure, and 5C of water was added to the residual liquid (260+nl) to prepare a synthetic polymer adsorbent, Amberlite XAD-7.
(Rohm and Haas) 3C was supplied to a column packed at a space velocity of 1e/LHr. After this, the column was washed with 109 water and then 50% ethanol 15Q was added at a space velocity I
The saponin was deposited on the skin by flowing with Q/Q-Hr, and the desorption solution was concentrated to dryness under reduced pressure to obtain 123 g of light brown purified saponin.

Polyglycerin fatty acid ester (Samp 7) Q-
17F; Taiyo Kagaku Co., Ltd. product) Ig was added, heated to 60°C, and stirred with a homomixer. Next, Emix 80 (preparation containing 80% natural tocopherol; manufactured by Eisai Co., Ltd.) Ig was added, and a homomixer (8000 rpm) was added.
m) for 2 minutes to emulsify.

The resulting emulsion was stable even after adjusting the pH to 4 with citric acid and heating in a boiling water bath for 15 minutes.

Comparative Example 2 Extract 3.5e was obtained from 200 g of Sapindica pericarp in the same manner as in Example 2, and ethanol was distilled off. Residual liquid 18
77 tons of 900 IIll was added to 0 ml, and the resulting precipitate was collected by filtration and dried under reduced pressure to obtain 24 g of purified saponin in the form of a yellowish brown powder.

Emix 80 was emulsified in the same manner as in Example 2 using 10 g of the purified saponin. A milky white emulsion was obtained, but when the pH was adjusted to 4 with citric acid and heated in a boiling water bath, the emulsion was destroyed in 5 minutes.

Example 3 Using the purified saponins obtained in Examples 1 and 2, the following! An oil-in-water emulsion was prepared.

75% high-fructose corn syrup 35g Refined saponin 15g Soybean lecithin 20g Olive oil 30g The resulting emulsions were all transparent and boiled in a boiling water bath for 30 g.
No breakage of the emulsion occurred even after heating for minutes. Further, when this was diluted 500 times with water, it was a transparent milky white straw that was stable for 30 minutes even after adjusting the pH to 4 with citric acid and heating to 90°C.

Comparative Example 3 Using the purified saponins obtained in Comparative Examples 1 and 2, an oil-in-water emulsion having the same composition as in Example 3 was prepared.

All the emulsions obtained were milky white and heated in a boiling water bath for 30 minutes.
When heated for a minute, oil-water separation occurred and the emulsification was destroyed.

Claims (2)

[Claims] (1) A saponin-containing extract obtained by extracting the bark of Quillaja saponaria or the pericarp of Sapindica with water or a hydrophilic organic solvent containing water is treated with a synthetic polymer adsorbent, and the saponin-containing extract is adsorbed on the polymer adsorbent. An oil-in-water emulsion in which an oil phase component is emulsified with purified saponin obtained by desorbing saponin with a water-containing hydrophilic organic solvent. (2) The oil-in-water emulsion according to claim 1, wherein the purified saponin is desorbed from a synthetic polymer adsorbent that has been washed with an alkaline aqueous solution before desorption treatment.