JPS6193958A - エンドトキシンの定量法 - Google Patents
エンドトキシンの定量法Info
- Publication number
- JPS6193958A JPS6193958A JP21492884A JP21492884A JPS6193958A JP S6193958 A JPS6193958 A JP S6193958A JP 21492884 A JP21492884 A JP 21492884A JP 21492884 A JP21492884 A JP 21492884A JP S6193958 A JPS6193958 A JP S6193958A
- Authority
- JP
- Japan
- Prior art keywords
- endotoxin
- time
- gel
- gelation
- point
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002158 endotoxin Substances 0.000 title claims abstract description 43
- 238000001879 gelation Methods 0.000 claims abstract description 17
- 230000000977 initiatory effect Effects 0.000 claims abstract description 13
- 241000239218 Limulus Species 0.000 claims abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims description 24
- 239000006166 lysate Substances 0.000 claims description 6
- 238000000149 argon plasma sintering Methods 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 10
- 238000011088 calibration curve Methods 0.000 abstract description 6
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 6
- 238000004364 calculation method Methods 0.000 abstract description 4
- 108010072542 endotoxin binding proteins Proteins 0.000 abstract description 3
- 238000002347 injection Methods 0.000 abstract description 3
- 239000007924 injection Substances 0.000 abstract description 3
- 238000013213 extrapolation Methods 0.000 abstract description 2
- 206010037660 Pyrexia Diseases 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 230000027950 fever generation Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 8
- 239000000758 substrate Substances 0.000 description 8
- 238000005259 measurement Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000012085 test solution Substances 0.000 description 4
- 241001529572 Chaceon affinis Species 0.000 description 3
- 210000000601 blood cell Anatomy 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000012480 LAL reagent Substances 0.000 description 2
- 239000000701 coagulant Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 1
- 208000037487 Endotoxemia Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000003677 hemocyte Anatomy 0.000 description 1
- 229940000351 hemocyte Drugs 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 239000003805 procoagulant Substances 0.000 description 1
- 239000002510 pyrogen Substances 0.000 description 1
- 230000001698 pyrogenic effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21492884A JPS6193958A (ja) | 1984-10-13 | 1984-10-13 | エンドトキシンの定量法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21492884A JPS6193958A (ja) | 1984-10-13 | 1984-10-13 | エンドトキシンの定量法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6193958A true JPS6193958A (ja) | 1986-05-12 |
| JPH0414310B2 JPH0414310B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1992-03-12 |
Family
ID=16663893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21492884A Granted JPS6193958A (ja) | 1984-10-13 | 1984-10-13 | エンドトキシンの定量法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6193958A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006076617A3 (en) * | 2005-01-13 | 2006-12-28 | Charles River Lab Inc | Method for classifying a microorganism in a biological sample |
| US7329538B2 (en) | 2003-03-17 | 2008-02-12 | Charles River Laboratories, Inc. | Methods and compositions for the detection of microbial contaminants |
| WO2008139544A1 (ja) * | 2007-05-01 | 2008-11-20 | Kowa Company, Ltd. | ゲル化測定装置および試料セル |
| WO2009116633A1 (ja) * | 2008-03-19 | 2009-09-24 | Obata Toru | ゲル粒子測定装置 |
| JP2010185849A (ja) * | 2009-02-13 | 2010-08-26 | Kowa Co | 生物由来の生理活性物質の測定方法及び測定装置 |
| US7968280B2 (en) | 2004-12-02 | 2011-06-28 | Charles River Laboratories, Inc. | Methods for the detection and/or quantification of gram positive bacterial contaminants |
| CN102112866A (zh) * | 2008-07-30 | 2011-06-29 | 兴和株式会社 | 用于测定来自生物体的生理活性物质的方法和测定装置 |
| WO2014058758A1 (en) * | 2012-10-08 | 2014-04-17 | General Electric Company | Sensitive and rapid method for detection of low levels of lal-reactive substances |
-
1984
- 1984-10-13 JP JP21492884A patent/JPS6193958A/ja active Granted
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10119969B2 (en) | 2003-03-17 | 2018-11-06 | Charles River Laboratories, Inc. | Compositions for the detection of microbial contaminants |
| US7329538B2 (en) | 2003-03-17 | 2008-02-12 | Charles River Laboratories, Inc. | Methods and compositions for the detection of microbial contaminants |
| US7939291B2 (en) | 2003-03-17 | 2011-05-10 | Charles River Laboratories, Inc. | Methods for the detection of microbial contaminants |
| US8440394B2 (en) | 2004-12-02 | 2013-05-14 | Charles River Laboratories, Inc. | Methods for the detection and/or quantification of gram positive bacterial contaminants |
| US7968280B2 (en) | 2004-12-02 | 2011-06-28 | Charles River Laboratories, Inc. | Methods for the detection and/or quantification of gram positive bacterial contaminants |
| US7901899B1 (en) | 2005-01-13 | 2011-03-08 | Charles River Laboratories, Inc. | Method for classifying a microorganism in a biological sample |
| WO2006076617A3 (en) * | 2005-01-13 | 2006-12-28 | Charles River Lab Inc | Method for classifying a microorganism in a biological sample |
| US7479375B2 (en) | 2005-01-13 | 2009-01-20 | Charles River Laboratories, Inc. | Method for classifying a microorganism in a biological sample |
| JP2008526257A (ja) * | 2005-01-13 | 2008-07-24 | チャールズ リバー ラボラトリーズ， インコーポレイテッド | 生体サンプル中の微生物を分類する方法 |
| WO2008139544A1 (ja) * | 2007-05-01 | 2008-11-20 | Kowa Company, Ltd. | ゲル化測定装置および試料セル |
| JP4551980B2 (ja) * | 2008-03-19 | 2010-09-29 | 徹 小幡 | ゲル粒子測定装置 |
| WO2009116633A1 (ja) * | 2008-03-19 | 2009-09-24 | Obata Toru | ゲル粒子測定装置 |
| JPWO2009116633A1 (ja) * | 2008-03-19 | 2011-07-21 | 小幡 徹 | ゲル粒子測定装置 |
| US8462340B2 (en) | 2008-03-19 | 2013-06-11 | Toru Obata | Gel particle measuring apparatus |
| CN102112866A (zh) * | 2008-07-30 | 2011-06-29 | 兴和株式会社 | 用于测定来自生物体的生理活性物质的方法和测定装置 |
| EP2306177A4 (en) * | 2008-07-30 | 2012-03-28 | Kowa Co | METHOD FOR MEASURING A PHYSIOLOGICAL ACTIVE DERIVED FROM AN ORGANISM AND CORRESPONDING MEASURING DEVICE |
| US8697351B2 (en) | 2008-07-30 | 2014-04-15 | Kowa Company, Ltd. | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| JP2010185849A (ja) * | 2009-02-13 | 2010-08-26 | Kowa Co | 生物由来の生理活性物質の測定方法及び測定装置 |
| CN104704369A (zh) * | 2012-10-08 | 2015-06-10 | 通用电气公司 | 用于检测低水平的lal反应物质的灵敏快速方法 |
| JP2015531489A (ja) * | 2012-10-08 | 2015-11-02 | ゼネラル・エレクトリック・カンパニイ | 低レベルのｌａｌ反応性物質を検出するための高感度かつ迅速な方法 |
| US9678079B2 (en) | 2012-10-08 | 2017-06-13 | General Electric Company | Microfluidic LAL-reactive substances testing method and apparatus |
| US9880166B2 (en) | 2012-10-08 | 2018-01-30 | General Electric Company | Sensitive and rapid method for detection of low levels of LAL-reactive substances |
| US10082505B2 (en) | 2012-10-08 | 2018-09-25 | General Electric Company | Centripetal microfluidic platform for LAL-reactive substances testing |
| WO2014058758A1 (en) * | 2012-10-08 | 2014-04-17 | General Electric Company | Sensitive and rapid method for detection of low levels of lal-reactive substances |
| US10302642B2 (en) | 2012-10-08 | 2019-05-28 | General Electric Company | Sensitive and rapid method for detection of low levels of LAL-reactive substances |
| US10352934B2 (en) | 2012-10-08 | 2019-07-16 | General Electric Company | Preloaded test substrates for testing LAL-reactive substances, methods of use, and methods of making |
| US10451622B2 (en) | 2012-10-08 | 2019-10-22 | Bl Technologies, Inc. | Centripetal microfluidic platform for LAL reactive substances testing |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0414310B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1992-03-12 |
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