JPS61501162A - How to determine agglutination reaction results - Google Patents

How to determine agglutination reaction results

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JPS61501162A
JPS61501162A JP50008783A JP50008783A JPS61501162A JP S61501162 A JPS61501162 A JP S61501162A JP 50008783 A JP50008783 A JP 50008783A JP 50008783 A JP50008783 A JP 50008783A JP S61501162 A JPS61501162 A JP S61501162A
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スオバニエミ，オスモ
エクホルム，ペルツチ
カウカネン，エスコ
パルタネン，パウル
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ラブシステムズ　オイ
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

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Abstract

(57)【要約】本公報は電子出願前の出願データであるため要約のデータは記録されません。 (57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

【発明の詳細な説明】 凝集反応結果の決定方法 本発明は、垂直測定分析機による凝集反応結果の測定方法に関するものである。[Detailed description of the invention] How to determine agglutination reaction results The present invention relates to a method for measuring agglutination reaction results using a vertical measurement analyzer.

各種の凝集試験が例えばバクテリア、ウィルス、細菌の抗原成分又はその抗体の 成分並びに異常蛋白質（例えば腫瘍−特殊蛋白質）の成分の検出のために使用さ れる。Various agglutination tests are used to test bacteria, viruses, bacterial antigen components or their antibodies. used for the detection of components as well as components of abnormal proteins (e.g. tumor-specific proteins). It will be done.

これらの試験の中で、血球凝集（ＨＡ）、血球凝集阻害（ＨＩ）、間接血球凝集 並びにラテックス凝集について述べなければならない。Among these tests, hemagglutination (HA), hemagglutination inhibition (HI), indirect hemagglutination Also, latex agglomeration must be mentioned.

前記試験は血清学上の診断、血清型決定、並びに抗原の各種検査において用いら れる。例えば、流行病の研究並びに感染病の特殊診断において、前記試験は非常 に有用である。The test is used in serological diagnosis, serotyping, and various antigen tests. It will be done. For example, in the study of epidemic diseases as well as in the special diagnosis of infectious diseases, the above tests are extremely important. It is useful for

血液型の決定において、赤血球細胞の凝集に基づく反応が使用される。In determining blood type, a reaction based on agglutination of red blood cells is used.

用いられる試験によって、凝集の正の結果は正の結果又は負の結果を意味し得る 。血球凝集は反応容器の底部上に広がった被覆として確認することができる。例 えば、ＨＡ−試験においては、ウィルス及びバクテリアは一定条件下で、反応容 器の底部上に大きな面積にわたって赤血球細胞を凝集させる能力を有する。血球 凝集が全く起らない場合は、赤血球細胞は通常反応容器内の明確に駆足された領 域として、いわゆるボタン状に集まる。Depending on the test used, a positive result for agglutination can mean a positive result or a negative result . Hemagglutination can be seen as a spreading coating on the bottom of the reaction vessel. example For example, in the HA-test, viruses and bacteria are tested in a reaction volume under certain conditions. It has the ability to aggregate red blood cells over a large area on the bottom of the vessel. blood cell If no agglutination occurs, red blood cells usually occupy well-defined areas within the reaction vessel. As a region, they gather in a so-called button shape.

さて、凝集試験の結果は目視によって判定されて来た＠異なる試験においては、 結果の解釈に対する基ＱＡは変わる。結果が目視によって判定された場合は、多 数の異なる誤差因子が含まれる。人によって見方も異なり、目は疲れ易く、そし て結果は変わり得るものであり、それ故、結果の解釈において人的誤差の要因は 大きい。凝集試験は又、色調指示薬と組み合わせることもできるがしかし、その 目視による判定は負又は正の試験においては困難なものである。Now, the results of the agglutination test have been judged visually @In different tests, The basis QA for interpretation of results varies. If the results are determined visually, A different number of error factors are included. People see things differently, and their eyes get tired easily. results are subject to change and, therefore, the source of human error in the interpretation of results is big. Agglutination tests can also be combined with color indicators; Visual judgment is difficult for negative or positive tests.

この種の試験は異なる測定に対して広く使用されている。又、非常に関係のある ものとしては多数行われる試験例えば梅毒及びリエーマチの試験、血液型の決定 、及び妊娠の判定が挙げられる。前記の場合において、試験の実施は簡単で、自 動的で、正確で且つ確実に再現可能なものでなければならない。This type of test is widely used for different measurements. Also, very relevant Many tests are carried out, such as tests for syphilis and riematosis, and determination of blood type. , and determination of pregnancy. In the above cases, conducting the test is simple and automatic. It must be dynamic, accurate and reliably reproducible.

今までは前記試験の実施の簡単且つ確実な自動的方法は全くなかった。Until now, there has been no easy and reliable automatic method of performing said tests.

−」えば、血液型を入手可能な装置（グルーパマティック”；　Ｇｒｏｕｐａｍ ａｔｉｃＲ）を使用して凝集反応を用いて決定する場合、例えば以下の理由： １、凝集反応混合物は測定前に強く振とうされるので該凝集反応混合物は反応混 合物中に分散される（イギリス国特許第１２２９９７１号）。- For example, a blood type device available (Groupamatic); aticR) for the following reasons: 1. The agglutination reaction mixture is shaken strongly before measurement, so the agglutination reaction mixture is (UK Patent No. 1,229,971).

Ｚ　反応混合物は２本の別々の測定用光線を用いて測定され（イギリス国時許４ １２２９９７１号及びアメリカ合衆国特許第５８８５５０８号）、これらによっ て得られる’、ｆ　ｉ″１２つの別ンつミＪＡと比こ（される。Z The reaction mixture was measured using two separate measuring beams (UK time permit 4). No. 1,229,971 and U.S. Pat. No. 5,885,508), It is compared with 12 different numbers JA.

によって、待ンζ弱い４集反応の解釈においてしばしｄ困魅を生ずる。This sometimes causes some confusion in the interpretation of the weak 4-group reaction.

凝集反応結果の形成、測定及び算出は、これまでのところいくつかの発明（イギ リス国特許第ｊ５３２０５７号、アメリカ合衆国特許第５７０７５５４号、及び アメリカ合衆国特許第４１４８６０７号）が水製してはいるけ几ども、まだ確実 且つ迅速に開明されてはいない。The formation, measurement and calculation of agglutination reaction results have so far been developed by several inventions (in Lisu Country Patent No. j532057, United States Patent No. 5707554, and U.S. Patent No. 4,148,607) is made of water, but it is still reliable. Moreover, it has not been discovered quickly.

しかしながら、良好な解決としては、ＦＰ−９分析系（ラブシステムズオイ、フ ィンランド国）を使用する装置が考えられ、そしてこれは特許（アメリカ合衆国 特許第４２９０９９７号、凝集試験結果の自動測定装置）中に開示されている。However, a good solution is the FP-9 analytical system (Lab Systems Oy, A device using a patent (United States of America) is considered, and this It is disclosed in Japanese Patent No. 4290997 (automatic measuring device for agglutination test results).

本発明及び前記先行発明による方法は例えば凝集反応結果の測定を、特に分光光 度計、吸着光度計、蛍光計及びネフエロメータ（ｎｅｆｅｌｏｍｅｔｅｒ　）を 用いて行う。The method according to the present invention and the prior invention can be used, for example, to measure the results of an agglutination reaction, especially using spectrophotometry. meter, adsorption photometer, fluorometer and nephelometer It is done using

本発明の方法は、例えば凝集反応結果の形成、測定及び算出を確実且つ迅速に自 動的に又は一部非自動的に行う。The method of the invention enables, for example, the formation, measurement and calculation of agglutination reaction results reliably and quickly. Dynamically or partially non-automatically.

本方法において、凝集は適度の遠心又は振とうによって促進される。その後反応 混合物は温置される。１直段階の後、反応混合物の振とぅが行われ、その結果、 弱い凝集混合物でさえも反応混合物中に分散されず、反応容器の底部上に残るが しかし、元号に強固なので、非凝集部は反応混合物中で均一に保たれることがで きる。短時間放置した後、反応混合物は測定される。反応混合物のキュベツトの 底部上に生じた凝集物の吸光度は（好ましくは例えば垂直光線測定を用いて）測 定されるので、その結果光源又は反応容器は水平面内で移動し、且つ同時に通過 光の強度は移動路に沿った複数の相で測定される。In this method, aggregation is promoted by moderate centrifugation or shaking. then react The mixture is incubated. After the first stage, shaking of the reaction mixture is carried out, so that Even weakly agglomerated mixtures are not dispersed in the reaction mixture and remain on the bottom of the reaction vessel; However, since the non-agglomerated portions are so strong that they can remain homogeneous in the reaction mixture, Wear. After standing for a short time, the reaction mixture is measured. of the reaction mixture cuvette. The absorbance of the aggregates formed on the bottom is measured (preferably using e.g. vertical beam measurement). As a result, the light source or reaction vessel moves in the horizontal plane and simultaneously passes through the The intensity of the light is measured in multiple phases along the path of travel.

複数の異なる点における一光線測定を用いて測定される吸光度の値は、プログラ ムに基づいて分析され且つ比較される。結果の算出は自動的に行われる。Absorbance values measured using single-ray measurements at several different points can be are analyzed and compared based on the system. Calculation of results is automatic.

以下の説明及び添付図面によシ本発明を更に詳細に説明するものであり、図中、 第１ａ図は反応混合物のキュベツトの底部上にある凝集物を示し、この場合結果 は正の状態である。The present invention will be explained in more detail with reference to the following description and accompanying drawings, in which: Figure 1a shows aggregates on the bottom of the cuvette of the reaction mixture, in this case the result is a positive state.

第１ｂ図は反応混合物のキュベツトの底部上にある凝集物を示し、この場合結果 は負の状態である。Figure 1b shows aggregates on the bottom of the cuvette of the reaction mixture, in this case the result is a negative state.

第２図は反応混合物のキュベツトの集合体の測定状態を図示し、 第３図は血液型の決定系の一実施態様を例示し、９７４４ａ図Ｆ′ｉ複数の異な る点におけるキュベツト底部の測定状態を図示し、そして 第４ｂ図は相当する測定結果を示す。FIG. 2 illustrates the measurement state of a collection of reaction mixture cuvettes, FIG. 3 illustrates one embodiment of a blood type determination system, and FIG. the measurement situation at the bottom of the cuvette at the point FIG. 4b shows the corresponding measurement results.

本文記載の方法、及び該方法による系において、ＡＢＯ−型及びＲｈ−因子の決 定は例えば以下のように行われる二１、ＡＢＯ−型の決定 赤血球細胞は凝果試談によって以下のように調べる二１）約２−５％の＋ｄｌ胞 響濁物を、生理食塩溶液中の検査すべき赤血球細胞から作る。In the method described in the text and in the system according to the method, determination of ABO-type and Rh-factor. For example, determination of ABO-type is carried out as follows. Red blood cells are examined by clotting test as follows: 21) Approximately 2-5% +dl cells. A sound suspension is made from the red blood cells to be examined in saline solution.

２）血液−型決定キュベツト中に、　ａ　−Ａ、　ａ　−Ｂ、　ａ　−ＡＢ　− 血清５０μｊをピペットで分取し、そして各キュベツト中に１）項で調製した赤 血球細胞懸濁液５０μノをピペットで分取する。2) In the blood type determination cuvette, a-A, a-B, a-AB- Pipette 50μj of serum, and add the red prepared in step 1) to each cuvette. Aliquot 50μ of the blood cell suspension using a pipette.

５）　１５秒間攪拌機中で攪拌する。5) Stir in a stirrer for 15 seconds.

４）　１０分〜１５分間室温で温置する。4) Incubate at room temperature for 10 to 15 minutes.

５）第５項に記載の条件下で攪拌する。5) Stir under the conditions described in Section 5.

６）　ＦＰ−血液型一分析機を用いて５４０ｎｍで測定する。6) Measure at 540 nm using a FP-Blood Type Analyzer.

装置は例えば相当する抗−血清１００μｌを用いて零に再設定する。The device is reset to zero using, for example, 100 μl of the corresponding anti-serum.

プラズマ／血清の外、抗体は以下の方法で凝集試験を用いて同様に調べる： １）約２．５壬の細胞懸濁物を、生理食塩溶液中のＡ−及びＢ−試験細胞から作 る。Besides plasma/serum, antibodies are similarly tested using an agglutination test in the following manner: 1) Approximately 2.5 μm cell suspension is made from A- and B-test cells in saline solution. Ru.

２）検査すべきプラズマ／血清５０μ！及び試験細胞懸濁物５０μノをピペット で分取する（第１項参照）。2) 50μ plasma/serum to be tested! and pipette 50μ of the test cell suspension. (See Section 1).

３）　１５秒間攪拌する。3) Stir for 15 seconds.

４）　混合物を１５分ないし２０分間室温で温置する。4) Incubate the mixture for 15 to 20 minutes at room temperature.

５）第３）項に記載の条件下で攪拌する。5) Stir under the conditions described in section 3).

６）５４００ｍで測定する（装置は例えばＢＳＡ−溶液１００μ）を用いて零に 再設定する）。6) Measure at 5400 m (equipment, e.g. BSA-solution 100μ) to zero. (reset).

１）約２．５チの懸濁物を、生理食塩溶液中の検介すべき赤血球細胞から作る。1) A suspension of about 2.5 cm is made of the red blood cells to be probed in saline solution.

２）１）で調製した懸濁物５０μｊ及びａ−Ｄ−血清５０μ！をＦＰ−血液−型 キュペット中にピペットで分取する。2) 50 μj of the suspension prepared in 1) and 50 μj of a-D-serum! FP-blood-type Pipette into a cupette.

３）１５秒秒間上う又は遠心する。3) Centrifuge or centrifuge for 15 seconds.

４）１５分間室温で温置する。4) Incubate at room temperature for 15 minutes.

５）　１５秒間攪拌する。5) Stir for 15 seconds.

６）波長５４０　ｎｍで測定する（装置は相当する抗−血清１００μＪｔ−用い て零に再設定する）。6) Measure at a wavelength of 540 nm (the device uses a corresponding antiserum of 100 μJt). (reset to zero).

Ｒｈ−制御 ＢＳＡ（２２％　ｉ　ｐＨ７，２）　５０μＪ検査すべき赤血球４細胞懸濁液　 ５０μ！前記第１項ないし第６項記載の操作手順を用いる。Rh-control BSA (22% i pH 7,2) 50μJ 4-cell suspension of red blood cells to be tested 50μ! The operating procedures described in Items 1 to 6 above are used.

装置は例えば８８人１００μノを用いて零に再設定されて１）２．５チ又は５チ の細胞懸濁物を洗浄された細胞から作る。The device can be reset to zero using e.g. A cell suspension is made from the washed cells.

２）　ａ−Ｄ−血清１００μ！及び２５チ又は５チ細胞懸濁物はＦＰ−血液−型 キュペット中にピペットで分取される。2) a-D-serum 100μ! and 25 CH or 5 CH cell suspensions are FP-Blood-type Pipette into a cupette.

３）　１５秒間攪拌する。3) Stir for 15 seconds.

４）混合物を＋５７℃で１５分間温置市る。4) Incubate the mixture at +57°C for 15 minutes.

５）この方法によって感作された赤血球細胞を生理食塩水を用いて３回洗浄する 。5) Wash the red blood cells sensitized by this method three times with physiological saline. .

６）　ａ−人一血清１００μＪを洗浄細胞に加える。6) a- Add 100 μJ of human serum to the washed cells.

７）　１５秒間攪拌する。7) Stir for 15 seconds.

８）１５秒秒間上う又は遠心する。8) Centrifuge or centrifuge for 15 seconds.

９）　１５秒間攪拌する。9) Stir for 15 seconds.

１０）５４０ｎｍで測定する（装置は例えばａ−人一血清１００μｍを用いて零 に再設定する）。10) Measure at 540 nm (for example, the device uses 100 μm of human serum ).

第１ａ図は反応−混合物キュベツト１の底部上の凝集物２を示し、結果はこの場 合上である。第１ｂ図は負の結果を示し、凝集に関しては、キュベツト３中では 、赤血球細胞は反応混合物４甲に一様に分散されている。Figure 1a shows aggregate 2 on the bottom of reaction-mixture cuvette 1; It's a coincidence. Figure 1b shows negative results, with respect to aggregation, in cuvette 3. , the red blood cells are uniformly dispersed in the reaction mixture 4A.

第２図は反応−混合物キュベツトの集合体５がどのようにして測定光線６を通過 し、その結果キュベツトの集合体５中の各反応−混合キエペット７が異なる点に おいて垂直測定光線６によって読み取られるかを図式的に示している。垂直測定 光線６は各反応−混合物キュペット７に面した適当な大きさの開口部８から発し 、反応−混合物キュベツト７の長尺軸の方向に通過して対応する検出器９に達す る。このように、各反応−混合物キュペット７の底部上の凝集物１０はＸ−軸方 向及び／又は必要ならばＹ−軸方向にも又キーペットの集合体５を適当に配置す ることによって、複数の点で測定され得るものである。もちろん、測定光線６Ｆ ｉ先端部から下って反応−混合物キュペット７の下部に配置された検出器に達す る反対方向にも通過し得るものでアシ、又は、キュベツトが静置され、光線がキ ュベツトの底部を横切って転位されてもよい。Figure 2 shows how the collection of reaction-mixture cuvettes 5 passes through the measuring beam 6. As a result, each reaction-mixture pet 7 in the cuvette assembly 5 has a different point. FIG. vertical measurement The light beam 6 emanates from an appropriately sized aperture 8 facing each reaction-mixture cupette 7. , passes in the direction of the longitudinal axis of the reaction-mixture cuvette 7 and reaches the corresponding detector 9. Ru. Thus, the aggregates 10 on the bottom of each reaction-mixture cupette 7 are aligned along the X-axis. The keypet assembly 5 is appropriately arranged in the direction and/or also in the Y-axis direction if necessary. can be measured at multiple points. Of course, the measurement beam 6F i descending from the tip to the detector located at the bottom of the reaction-mixture cupette 7. The reed or cuvette is placed stationary so that the light beam can pass in the opposite direction. It may also be transposed across the bottom of the tube.

光線は複数の点でキュベツト底部を横切って通過し得る（第４ａ図参照）。参考 数字１１．１２及び１３は異なる移動通路を現わす。第４ｂ図は相当する測定結 果を示す。第４ｂ図において、曲縁１５は例えば光度計で測定された場合の点１ ２における測定に相当する。The light beam may pass across the bottom of the cuvette at multiple points (see Figure 4a). reference Numbers 11, 12 and 13 represent different travel paths. Figure 4b shows the corresponding measurement results. Show results. In FIG. 4b, the curved edge 15 is, for example, at point 1 when measured with a photometer. This corresponds to the measurement in 2.

更に、攪拌機を尤度計測定ヘッドと接続して配置するように装置を構成すること ができる。この場合は、前記における出力は例えば振とう強度の関数として算出 することができるので、これによって異なる凝集の強さの間の相違が測定される 。Furthermore, the apparatus is configured such that the stirrer is connected and arranged with the likelihood meter measurement head. Can be done. In this case, the output in the above is calculated as a function of the shaking intensity, for example. This measures the difference between different agglomeration strengths, since .

得られた結果の計算のた′めに、装置には適当なコンビエータ装置を設置する（ 第３図Ｐ）。For the calculation of the results obtained, the device is equipped with a suitable combinator device ( Figure 3 P).

第３図は血液型決定のだめの本発明の方法の実施態様としての系を示す： ａ、全一血液試料 す、血液試料を遠心する。FIG. 3 shows a system as an embodiment of the method of the invention for blood group determination: a. Whole blood sample Centrifuge the blood sample.

Ｃ，プラズマ又は血清及び赤血球細胞を管内で分離する。C. Separate plasma or serum and red blood cells in the tube.

ｄ、管を矢印で示す場所に置く。d. Place the tube in the location indicated by the arrow.

ｅ、赤血球細胞２５μｍを管から取シ田し、所定を一希釈剤中に取シ込む。e. Remove 25 μm of red blood cells from the tube and take the specified amount into diluent.

ｆ、所定量−希釈剤を空の管中に移す。f. Transfer the predetermined amount of diluent into an empty tube.

ｇ−所定ｉｋ−希釈剤を用いて、試薬１０００μｍを加える。g - Add 1000 μm of reagent using the specified ik diluent.

ｈ、細胞懸濁ｗ５０μｊを所定型−希釈剤によって４個の反応−混合物キュベツ ト中に分取する。h, cell suspension w 50μj was added to 4 reaction-mixture cuvettes by the prescribed diluent. aliquot it during the test.

ｉ、プラズマ２００μｊを第２の所定量−希釈剤中に取シ込む。i. Inject 200 μj of plasma into a second predetermined amount of diluent.

ｊ、所定量−希釈剤（２）から、プラズマ５０μ！を並んだ４個の反応−混合物 キュベツトの各々中に分取する。j, predetermined amount - from diluent (2), plasma 50μ! 4 reactions in a row - mixture Aliquot into each cuvette.

ｋ、試薬５０μｍを反応−混合物キュベツト中に加える。k. Add 50 μm of reagent into the reaction-mixture cuvette.

１、反応−混合物キュベツトを温置する。1. Incubate the reaction-mixture cuvette.

ｍ、必要ならば、遠心又は振とりする。m. Centrifuge or shake if necessary.

ｎ、Ｊ％集集合合物反応混合物中に分散しな＾ように振とうする。振とうの間、 非凝集赤血球細胞を反応混合物中に分散する。Shake to avoid dispersion in the reaction mixture. During shaking, Disperse non-agglutinated red blood cells into the reaction mixture.

０、一定時間経過後、振とうに続いて各反応−混合物キニベットの底部を通過す る５ないし１０の異なる点において垂直測定光線を用いて自動的に測定する。0. After a certain period of time, each reaction-mixture is passed through the bottom of the kinivet following shaking. automatically measure using a vertical measuring beam at 5 to 10 different points.

ｐ、記憶、計算、分析及び測定結果の算出。p, memory, calculation, analysis and calculation of measurement results.

本発明の方法の応用として、例えば自動化の程度の異なる、又は機械的様式の異 なる系を構成することができる。このような系は一装置又は複数の別々の装置に よって構成され得る。結果の計算及び分析にあたり、測定結果は例えば標準値と 、又は互いに、及び／又は正若しくは負の結果と、又はその両方と比較すること ができる。Applications of the method of the invention may include, for example, different degrees of automation or different mechanical modes. A system can be constructed. Such systems can be installed in one device or in several separate devices. Therefore, it can be configured. When calculating and analyzing the results, the measurement results may be compared with standard values, for example. or with each other and/or with positive or negative results. Can be done.

ｐｏｓ、　ｎｅｇ 補正書の翻訳文提出書 （特許法第１８４条の７第１項） 昭和６０年７月２０日pos, neg Submission of translation of written amendment (Article 184-7, Paragraph 1 of the Patent Act) July 20, 1985

Claims (4)

【特許請求の範囲】[Claims] （１）光源又は反応容器を水平面上で転位し、同時に通過する光の強度を、移動 略に沿った複数の異なる点において測定することを特徴とする、光源から出て該 反応容器の底部を通過する光の強度を測定することよりなる凝集反応結果の決定 方法。(1) Transpose the light source or reaction vessel on a horizontal plane and simultaneously shift the intensity of the passing light. emitted from a light source and measured at a plurality of different points along approximately Determination of the agglutination reaction result by measuring the intensity of light passing through the bottom of the reaction vessel Method. （２）光の強度を移動の間中絶えず測定することを特徴とする請求の範囲第１項 記載の方法。(2) Claim 1, characterized in that the intensity of the light is constantly measured throughout the movement. Method described. （３）反応容器が転位し、光源が固定されていることを特徴とする請求の範囲第 １項又は第２項記載の方法。(3) Claim No. 1 characterized in that the reaction vessel is rearranged and the light source is fixed. The method described in Section 1 or Section 2. （４）反応容器が、測定の間複数の平行な直線に沿って光源に対して転位するこ とを特徴とする請求の範囲第１項ないし第３項のうちいずれか１項記載の方法。(4) The reaction vessel is dislocated with respect to the light source along multiple parallel straight lines during the measurement. The method according to any one of claims 1 to 3, characterized in that:
JP50008783A 1983-11-21 1983-11-21 How to determine agglutination reaction results Pending JPS61501162A (en)

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DE3422616A1 (en) * 1984-06-19 1985-12-19 Behringwerke Ag, 3550 Marburg METHOD FOR DETERMINING A PARTNER OF A REACTION AND DEVICE THEREFOR
US4730921A (en) * 1985-10-31 1988-03-15 Genetic Systems, Inc. Photodensitometer for minimizing the refractive effects of a fluid sample
EP0229355A3 (en) * 1986-01-06 1988-01-07 Orion Corporation Ltd Apparatus and method for carrying out photometric assays
DE3919260A1 (en) * 1989-06-13 1990-12-20 Hoechst Ag METHOD FOR QUANTITATIVELY EVALUATING AGGLUTINATION REACTIONS
CA2123644C (en) * 1993-05-17 2002-03-26 Tomoo Saito Method and apparatus for indirect agglutination immunoassay

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DE1598944A1 (en) * 1966-07-21 1971-06-24 Pfizer & Co C Method and device for the automatic detection of agglutinations in a reaction zone
FR95147E (en) * 1967-05-12 1970-07-24 Centre Nat Rech Scient Apparatus intended more particularly for the automatic determination of blood groups.
FI56905C (en) * 1978-02-28 1980-04-10 Osmo A Suovaniemi FOERFARANDE OCH ANORDNING FOER AUTOMATISK MAETNING AV AGGLUTINATIONSPROV T EX I SPEKTROPOTOMETER ADSOPTIONSFOTOMETER FLUOROMETER ELLER NEFELOMETER
JPS6145479Y2 (en) * 1979-09-10 1986-12-20
WO1982000355A1 (en) * 1980-07-24 1982-02-04 Oy Labsystems Method and apparatus for the measurement of the properties of an agglutination
EP0056416A1 (en) * 1980-07-24 1982-07-28 Labsystems Oy Method and apparatus for the measurement of the properties of an agglutination
WO1982000354A1 (en) * 1980-07-24 1982-02-04 Oy Labsystems Method and apparatus for the measurement of the properties of an agglutination
EP0056058B1 (en) * 1980-07-24 1985-06-05 Labsystems Oy Method and equipment for the measurement of properties of a liquid
FR2488691A1 (en) * 1980-08-14 1982-02-19 Commissariat Energie Atomique METHOD AND DEVICE FOR DETECTION AND QUANTIFICATION OF REAL-TIME AGGLUTINATES
FR2509860A1 (en) * 1981-07-17 1983-01-21 Louis Serge Measuring agglutination of red blood cells for blood grouping etc. - to give numerical factor calculated from multipoint opacity determination

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