JPS6139621B2 - - Google Patents
Info
- Publication number
- JPS6139621B2 JPS6139621B2 JP52076435A JP7643577A JPS6139621B2 JP S6139621 B2 JPS6139621 B2 JP S6139621B2 JP 52076435 A JP52076435 A JP 52076435A JP 7643577 A JP7643577 A JP 7643577A JP S6139621 B2 JPS6139621 B2 JP S6139621B2
- Authority
- JP
- Japan
- Prior art keywords
- free
- thyroid hormone
- inhibitor
- difference
- binding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000009739 binding Methods 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 26
- 239000005495 thyroid hormone Substances 0.000 claims description 26
- 229940036555 thyroid hormone Drugs 0.000 claims description 26
- 230000027455 binding Effects 0.000 claims description 25
- 239000003112 inhibitor Substances 0.000 claims description 20
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 claims description 17
- 238000003018 immunoassay Methods 0.000 claims description 15
- 210000002966 serum Anatomy 0.000 claims description 12
- 238000003127 radioimmunoassay Methods 0.000 claims description 8
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical group [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 8
- 102000004506 Blood Proteins Human genes 0.000 claims description 6
- 108010017384 Blood Proteins Proteins 0.000 claims description 6
- FWEOQOXTVHGIFQ-UHFFFAOYSA-N 8-anilinonaphthalene-1-sulfonic acid Chemical compound C=12C(S(=O)(=O)O)=CC=CC2=CC=CC=1NC1=CC=CC=C1 FWEOQOXTVHGIFQ-UHFFFAOYSA-N 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 229940033663 thimerosal Drugs 0.000 claims description 4
- 239000007790 solid phase Substances 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 2
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 230000010807 negative regulation of binding Effects 0.000 claims 1
- 229940035722 triiodothyronine Drugs 0.000 claims 1
- 238000000502 dialysis Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- 230000002159 abnormal effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000002572 Alpha-Globulins Human genes 0.000 description 1
- 108010068307 Alpha-Globulins Proteins 0.000 description 1
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- CXOFVDLJLONNDW-UHFFFAOYSA-N Phenytoin Chemical compound N1C(=O)NC(=O)C1(C=1C=CC=CC=1)C1=CC=CC=C1 CXOFVDLJLONNDW-UHFFFAOYSA-N 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 101710194518 T4 protein Proteins 0.000 description 1
- 102000009488 Thyroxine-Binding Proteins Human genes 0.000 description 1
- 108010048889 Thyroxine-Binding Proteins Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 229940064790 dilantin Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 229940034208 thyroxine Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/78—Thyroid gland hormones, e.g. T3, T4, TBH, TBG or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Endocrinology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/701,192 US4046870A (en) | 1976-06-30 | 1976-06-30 | Assay for free thyroid hormones |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS533516A JPS533516A (en) | 1978-01-13 |
| JPS6139621B2 true JPS6139621B2 (show.php) | 1986-09-04 |
Family
ID=24816404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7643577A Granted JPS533516A (en) | 1976-06-30 | 1977-06-27 | Measurement of concentration of free thyroid hormon within liquid sample |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4046870A (show.php) |
| JP (1) | JPS533516A (show.php) |
| CA (1) | CA1064825A (show.php) |
| DE (1) | DE2725594C2 (show.php) |
| FR (1) | FR2356943A1 (show.php) |
| GB (1) | GB1522566A (show.php) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1206354B (it) * | 1977-03-10 | 1989-04-21 | Lepetit Spa | Corredo da usarsi per la determinazione degli ormoni liberi nei fluidi biologici |
| US4292296A (en) * | 1978-09-12 | 1981-09-29 | Baxter Travenol Laboratories, Inc. | Diagnostic method |
| US4381291A (en) * | 1979-02-23 | 1983-04-26 | Ab Fortia | Measurement of free ligands |
| US4366143A (en) * | 1979-09-24 | 1982-12-28 | Amersham International Public Limited Company | Assay for the free portion of substances in biological fluids |
| US4391795A (en) * | 1980-03-21 | 1983-07-05 | Becton Dickinson And Company | Assay for free thyroid hormone |
| US4410633A (en) * | 1980-09-25 | 1983-10-18 | Corning Glass Works | Method for the measurement of free thyroxine or 3,5,3'-triiodothyronine in a liquid sample |
| US4468469A (en) * | 1981-11-04 | 1984-08-28 | Miles Laboratories, Inc. | Substituted phenylacetic acids and salts as TBP blocking agents in iodothyronine immunoassays |
| SE8200442L (sv) * | 1982-01-27 | 1983-07-28 | Forsvarets Forsknings | Forfarande vid detektion av organiska molekyler, sasom biomolekyler |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3745211A (en) * | 1971-04-08 | 1973-07-10 | Mallinckrodt Chemical Works | Method for determining thyroxine in blood serum and reagent therefor |
| US3928553A (en) * | 1972-03-23 | 1975-12-23 | Univ New York | Radioimmunoassay method for triiodothyronine and thyroxine |
| US3911096A (en) * | 1972-06-23 | 1975-10-07 | Professional Staff Ass Of The | Radioimmunoassay for measurement of thyroxine (T{HD 4{B ) and triiodothyonine (T{HD 3{B ) in blood serum |
| IL42105A (en) * | 1973-04-25 | 1976-08-31 | Yissum Res Dev Co | Process for determination of triiodothyronine |
| US3962039A (en) * | 1974-08-08 | 1976-06-08 | Center For Laboratory Medicine | Analytical procedure for thyroid hormones |
-
1976
- 1976-06-30 US US05/701,192 patent/US4046870A/en not_active Expired - Lifetime
-
1977
- 1977-04-18 CA CA276,339A patent/CA1064825A/en not_active Expired
- 1977-06-07 DE DE2725594A patent/DE2725594C2/de not_active Expired
- 1977-06-27 JP JP7643577A patent/JPS533516A/ja active Granted
- 1977-06-28 GB GB26967/77A patent/GB1522566A/en not_active Expired
- 1977-06-29 FR FR7719926A patent/FR2356943A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS533516A (en) | 1978-01-13 |
| US4046870A (en) | 1977-09-06 |
| GB1522566A (en) | 1978-08-23 |
| FR2356943A1 (fr) | 1978-01-27 |
| DE2725594C2 (de) | 1986-04-10 |
| FR2356943B1 (show.php) | 1983-01-21 |
| DE2725594A1 (de) | 1978-01-05 |
| CA1064825A (en) | 1979-10-23 |
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