JPS6131958A - Meter for quantitative determination of blood component - Google Patents

Abstract

PURPOSE:To execute quickly and easily the operations from blood collecting up to the flow passage for a carrier liquid and to reduce cost by mounting a capillary in which blood is collected to the injecting port of a valve device and injecting the blood into the flow passage for the carrier liquid. CONSTITUTION:The top end of the capillary tube 46 is touched to the blood bleeding out from the ear by giving a slight injury to the ear to collect the blood. A valve body holder 1 is moved toward the right against a coil spring 12 to position one end of the tube 46 to the injecting port 6a and the other end to a communicating port 6b in the stage of setting such tube 46 to the valve body holders 1, 2. A coil spring 12 is then extended to return the holder 1 to the home position in an arrow Y direction. Both valve bodies 5a, 5b are then pushed and retreated by the tube 46 and are opened by overcoming the force of coil springs 7a, 7b. Connecting ports 10a, 10b are closed by the retreat of the valves 5a, 5b and the carrier liquid flowing in a connecting tube 3 is transferred together with the blood through a tube 54, a slit 11b, a groove 9b, the tube 46 and a slit 11a by a tube 55 to the two electrode sides.

Description

[Detailed Description of the Invention] [Technical Field] The present invention enables the determination of test substances such as glucose, cholesterol, and uric acid in blood by eliminating the influence of interfering substances that cause errors in the determination of these test substances. The present invention relates to a blood component quantitative meter that can be used under various conditions.

[Background technology]

In the past, for example, the patent application filed in 1982-1 related to the applicant's patent application
As disclosed in No. 93294 (issued October 14, 1982), an injector is interposed in the middle of the channel through which the carrier liquid flows, a rubber valve is attached to this injector, and the carrier liquid channel is However, when injecting carrier liquid, test substance, etc., a syringe needle (micro cylinder) is used.
It was common practice to pierce the rubber valve and inject by pushing.

Blood samples from the human body are usually taken in blood collection tubes or capillary tubes. Conventionally, blood was collected from a blood collection tube or capillary using a microcylinder and injected into a blood component quantitative meter.

That is, separate devices are used for blood collection and injection, which requires time and effort to transfer, requires a large number of microcylinders, and requires high costs for storage and disinfection.

Note that since the capillary tube has a small diameter, it sucks in blood through capillary action.

[Purpose of the invention]

The purpose of this invention is to solve the above-mentioned conventional problems and collect (
The operation from blood collection to injection into the carrier liquid flow path is quick.
It is an object of the present invention to provide a blood component quantitative meter that is easy to perform and is advantageous in terms of cost.

[Disclosure of the invention]

The blood component quantitative meter of the present invention includes: a flow path through which a carrier liquid of a test substance flows; an inlet for an interfering substance into the flow path;
an inlet for the test substance into the flow channel; an interfering substance detection electrode inserted into the flow channel downstream from the interfering substance injection port; an inserted electrode for detecting an analyte, a calculation unit that corrects the amount of electricity output from the electrode for detecting the analyte based on the amount of electricity output from the electrode for detecting a disturbing substance, and a display unit for displaying the calculation results of the calculation unit; , comprising a capillary tube for collecting a test substance, etc., which is detachably attached to the injection port, and a valve device, which is attached to the injection port, for injecting the test substance, etc. in the capillary tube into the flow path. It is something.

According to the configuration of this invention, the following effects are achieved.

That is, blood is collected from a human body using a capillary tube. Alternatively, collect the standard solution from the reagent bottle. Rather than inhaling the test substance, etc. from the capillary from which the test substance has been collected using a micro cylinder, the sampled capillary itself is directly attached to the injection port, and the valve device is operated. Inject the test substance etc. directly into the carrier liquid flow path.

Therefore, the conventional work of transferring the capillary tube to the micro cylinder can be omitted. Furthermore, the need for a large number of microcylinders, their storage, and disinfection can be eliminated, leading to significant cost reductions.

Embodiment A first embodiment of the present invention will be described with reference to FIGS. 1 to 6.

FIG. 1 is a block diagram of detection, calculation, and display of a test substance. 20 is a constant voltage generation circuit, 21 is a current detection unit for the test substance detection electrode 37 (hereinafter also referred to as “Sens”), and 22 is an interfering substance detection electrode 38 (hereinafter referred to as “Sens”).
(also referred to as a sensor), 23A, 23
B is a current/voltage conversion circuit, 24A and 24B are amplifier circuits,
25A, 25B are filter circuits, 26A, 26B are A/
A D conversion circuit, 27 is an arithmetic circuit (arithmetic unit), and 28 is a display unit. The calculation circuit 27 performs calculation 5=a-b based on the detected electricity amount (digital amount) a of the test substance and the detected electricity N (digital amount) b of the interfering substance, and the display unit 28 displays the calculated value S. to be displayed digitally.

FIG. 2 is an overall configuration diagram of the blood component quantitative meter.

This blood component quantitative meter is composed of a storage tank 30 for a carrier liquid (buffer solution) 29, a metering pump 31, a valve device 32, an i-stream damper 33, a sensor section 34, a waste liquid tank 35, and a flow path 36 that connects these in order. has been done. Sensor part 3
4 is an electrode 37 for detecting an analyte, and an electrode 3 for detecting an interfering substance.
8 and counter electrodes 39 thereof, each of which is connected to a constant voltage generating circuit (battery) 20.20 via an ammeter 40.40. The specific structure of the valve device 32 will be described later.

It also has an analog data input section 41 for detecting current, and separate sensitivity two-stage switching circuits 42 and 42, which are connected to the A/D.
The central processing unit (CPU) 27 of the 8-pin microcomputer via the conversion circuit 26 (26A, 26B in FIG. 1)
(the arithmetic circuit 27 in Fig. 1), and the LED
It is connected to the digital display section 28 and various pilot display sections 44 via a (light emitting diode) driver 43 . 46 is a capillary.

FIG. 3 shows the front panel 51 of the main body of the quantitative meter.

This front panel 51 is provided with a digital display section 28, various pilot display sections 44, a connection port 47 for a carrier liquid suction tube 52, a connection port 48 for a waste liquid tube 53, a power switch 49, and a pump switch 50. The pilot display unit 44 has LEDs indicating an injection waiting instruction 44a, a measuring 44b, and a first reference liquid injection instruction 44C.
1 Second standard solution injection instruction 44d, sample solution injection instruction 44e
, and a sensor replacement instruction 44f are provided.

Next, an explanation will be given of the operation when determining the blood sugar level, that is, glucose using this quantitative meter.

■ When the power switch 49 is turned on, the metering pump 31 is activated and 1. Carrier liquid 29 having an H of about 7.5 flows into channel 36 . The flow rate is 3 ml and 11 minutes. The LED 44a indicating the wait for injection blinks until the carrier liquid 29 reaches the sensor section 34 (about 2 minutes).

(2) When the carrier liquid 29 reaches the sensor section 34, the LED 44C indicating the injection of the first reference liquid blinks.

According to this, the first
A glucose-only solution, which is a reference solution, is injected at the valve device 32 . This lights up the LED 44b during measurement. Electrode for detecting analyte (i.e., immobilized enzyme electrode)
37 is obtained, and the digital display section 28 shows, for example, 2.
It is displayed as 50 (a = 250 [mg/d1
)), there is no output from the interfering substance detection electrode 38. The sensitivity of the test substance detection electrode 37 to glucose at this time is stored in the memory of the microcomputer.

Note that if the sensitivity of the electrode 37 is low, the LBD 44C will blink, so a solution containing only glucose is injected again. This LED
As 44C blinks, the analog circuit is automatically switched to a circuit with a higher amplification degree in the two-stage sensitivity switching circuit 42, 42. Furthermore, when the sensitivity is very low, the LED 44f indicating a sensor replacement instruction flashes, and the sensor section 34 is replaced accordingly.

■ If the sensitivity of electrode 37 is appropriate, L E D44C
does not blink, and the LED 44d indicating the second reference liquid injection instruction blinks. Accordingly, a solution containing only an interfering substance (for example, ascorbic acid or uric acid), which is a second reference solution, is added to the injection port 6a.
Inject into. This lights up the LED 44b during measurement. Outputs from both the analyte detection electrode 37 and the interfering substance detection electrode 38 are obtained.

If the sensitivities of the two electrodes 37 and 38 are the same, the output is the same, and is displayed on the digital display section 28 as, for example, 70 (b = 70 (mg/a)).

However, normally the two sensitivities are different and the sensitivities are adjusted. The sensitivity of each electrode 37, 38 to interfering substances at this time is stored in the memory of the microcomputer.

Based on the above, 1. Both electrodes using the second reference solution 37.3
The calibration of the sensitivity of No. 8 is completed. Then, the calibrated numerical value is displayed on the digital display section 28, for example, 70 (b
=70C■/d1]).

■ After the above display, the LED 44e indicating sample solution injection
flashes. According to this, blood as a sample solution (test substance) is injected from the injection port 6a.

As a result, outputs are obtained from the two electrodes 37 and 38, and the microcomputer's CPU (arithmetic circuit, arithmetic unit) 27 performs the calculation of 5 = a - b, and the result is S, glucose % (blood sugar in 1 dI). The value ■) is displayed on the digital display section 28.
will be displayed. However, the values of a and b at this time are ■, ■
a(-250) at.

b(-70) does not necessarily match. This is because the composition differs depending on blood.

In addition, when the two electrodes 37 and 38 have different sensitivities to interfering substances, the sensitivity adjustment is performed based on the sensitivity memorized in ①.

Next, the structure and operation of the valve device 32 will be explained in Figures 4 to 6.
This will be explained based on the diagram.

1 is a first valve body holder on the sensor 37, 38 side;

Reference numeral 2 designates a second valve body holder on the side of the pump 31, and both the valve body holders 1 and 2 are communicated with each other by a connecting pipe 3 connected between the respective connecting portions 1a and 2-a. 1b is a sensor 37,
2b is a connection part of the tube 54 on the pump 31 side.

The first . Second valve chambers 4a and 4b are formed, and each valve chamber 4
a, 4b so as to be slidable. The second valve bodies 5a, 5b are inserted.

The first valve body holder 1 has an inlet 6a communicating with the valve chamber 4a.
is formed in the second valve body holder 2, while a valve chamber 4 is formed in the second valve body holder 2.
A communication port 6b is formed which communicates with b. The injection port 6a and the communication port 6b fit one end and the other end of the capillary tube 46, respectively, and are made of an elastic material such as rubber to prevent leakage.

In each valve chamber 4a, 4b, the valve bodies 5a, 5b are normally connected to the inlet 5a by coil springs 7a, 7b.

The communication port 6b is biased to close. Valve body 5a
, 5b are formed with O-rings 8a, 8b to ensure liquid tightness, and with the end of the capillary tube 46 in contact with the tip surface, the valve chamber 4a, 4
A groove 9a is formed on the distal end surface of the capillary tube 46 so that the tip b communicates with the capillary tube 46.
9b is formed.

When the coil springs 7a and 7b are extended and the valve bodies 5a and 5b close the inlet 5a and the communication port 6b (Fig. 4), the valve chambers 4a and 4b are connected to the connecting pipe 3. The ports 10a and 10b are open, and the carrier liquid 29 flows from the tube 54 to the valve chamber 4b→the connecting pipe 3→the valve chamber 4b→the tube 55.

Each valve body 5a, 5b has a slit ll penetrating its peripheral wall in the sliding direction at a position different from that of the connection ports 10a, 10b.
a, llb are formed.

The first valve body holder 1 is held by a coil spring 12 interposed between the first valve body holder 1 and a fixing portion 56 so as to be able to move forward and backward.

The capillary tube 46 is connected to the first. To set the second valve body holder 1.2, first, move the first valve body holder 1 in the direction of arrow X against the coil spring 12, and then set both valve body holders 1.2 to the second valve body holder 1.2.
A gap larger than the length of the capillary tube 46 is provided between the two.

Next, one end of the capillary tube 46 is aligned with the injection port 6a and the other end with the communication port 6b, and the coil spring 12 is expanded.
As shown in FIG. 6, when the first valve body holder 1 is returned to its original position as indicated by arrow Y, both valve bodies 5a and 5b are moved back by the force of the capillary tube 46, and the coil spring 7a is pushed back.

The valve is opened against 7b. Both valve bodies 5a and 5b are opened simultaneously. Furthermore, the connection ports 10a and 10b are closed due to the retraction of the valves 5a and 5b, and the carrier liquid 29 no longer flows into the connection pipe 3. That is, the carrier liquid 20 is transferred from the tube 54 to the slit Ilb to the groove 9b to the capillary tube 46.
→Flows from groove 9a to slit 1la to tube 55. Due to the flow of this carrier liquid, the test substance (blood) in the capillary tube 46 flows toward the sensors 37 and 38.

In the above operation, both ends of the capillary tube 46 are fitted into the injection port 6a and the communication port 6b, and the valve body 5a is closed.

Since the valves 5b and 5b open at the same time, there is an advantage that leakage of the test substance and the carrier liquid does not occur.

In practice, the capillary tubes containing the first reference solution containing only glucose are set, the capillary tubes containing the second reference solution containing only interfering substances are set, and the capillary tubes containing blood are set in this order.

The capillary tube 46 has an outer diameter of 5 ++ m to 2.0 m.
11m, inner diameter 0.8 to 1.4 cranes, length 75 to 13
011 It has the true dimensions, and blood is collected by making a small cut in the ear and applying the tip to the blood that comes out.

In this embodiment, when the capillary tube 46 is placed in the first and second valve body holders 1.2 (see FIG. 6),
Connection port 10a is provided by valve bodies 5a and 5b.

Instead of being completely closed, the connection port 10a.

10b is partially in communication with the carrier liquid 2 in the connecting pipe '3.
9 may be allowed to flow. This is effective in keeping the flow rate of the carrier liquid 29 constant before and after the center of the capillary tube 46 when the inner diameters of the capillary tube 46 and the connecting tube 3 are different.

A second embodiment will be described based on FIGS. 7 and 8.

The valve device 32 of this embodiment has a cylinder 14 having a piston 13, and a normally closed valve that opens toward the cylinder chamber 14a is provided in a communication portion 17a between the cylinder chamber 14a and an injection port 16 made of an elastic material. A pipe body 19 is provided with one check valve 18a and constitutes a part of the cylinder chamber 14a and the carrier liquid flow path 36.
A normally closed second check valve 18b that opens toward the flow path 36a is provided in a communication portion 17b with the flow path 36a inside.

57 is a ventilation hole.

The rest is the same as the first embodiment, so the explanation will be omitted.

After fitting the capillary tube 46 containing the test substance etc. into the injection port 16 as shown in FIG.
8a opens, and the test substance etc. in the capillary tube 46 enters the cylinder chamber 1.
4a is inhaled. At this time, the second check valve 18b is closed.

Next, when the piston 13 is pushed down as shown in FIG. 8(B), the first check valve 18a closes and the pressure in the cylinder chamber 14a increases, so the second check valve 18b opens and the pressure in the cylinder chamber 14a increases. A test substance or the like is injected into the flow path 36a.

Note that some test substances remain in the communication portion 17a, so before the next use, move the piston 13 up and down once to remove all the remaining test substances into the flow path 36.
and then start measuring.

In the above embodiment, the injection port for the interfering substance and the injection port for the test substance are the same, but an embodiment in which they are provided separately is also an embodiment of the present invention.

〔Effect of the invention〕

According to this invention, instead of inhaling the test substance etc. with a micro cylinder from the capillary tube from which the test substance etc. was collected (the collected capillary tube itself is attached directly to the injection port and the valve device is operated, the capillary tube is inhaled). Since the test substance, etc. in the container is injected directly into the carrier liquid flow path, the conventional work of transferring from a capillary tube to a micro cylinder can be omitted, and the process of storing and disinfecting a large number of micro cylinders, which was previously required, can be omitted. This has the effect of making it possible to eliminate the need for other items, resulting in significant cost reductions.

[Brief Description of the Drawings] Fig. 1 is a block diagram of the first embodiment of the present invention, Fig. 2 is a schematic configuration diagram thereof, Fig. 3 is a front view of the panel front plate, and Fig. 4 is a diagram of the valve device. 5 is a front view of the valve body, FIG. 6 is a sectional view explaining the operation of the valve device, FIG. 7 is a sectional view of the valve device of the second embodiment, FIG. 8 (A), (B) is a sectional view explaining the operation. DESCRIPTION OF SYMBOLS 1... First valve body holder, 2... Second valve body holder, 3... Connection pipe, 5a... First valve body, 5b...
Second valve body, 6a, 1.6... Inlet, 6b...
・Communication port, 10a.

Claims (4)

[Claims] (1) A flow path through which a carrier liquid of a test substance flows, an injection port for an interfering substance into this flow path, an injection port for the test substance into the flow path, and a flow path downstream of the interfering substance injection port. an interfering substance detection electrode inserted into the analyte, an analyte detection electrode inserted into the flow path on the downstream side of the analyte injection port, and an interfering substance detection electrode inserted into the flow path, and the output electricity of the analyte detection electrode from the interference. a calculation unit that performs correction based on the amount of electricity output from the substance detection electrode; a display unit for displaying the calculation results of the calculation unit; a capillary tube for collecting a test substance etc. that is detachably attached to the injection port; A blood component quantitative meter comprising: a valve device attached to an inlet for injecting a test substance, etc. in the capillary tube into the flow path. (2) the valve device includes a first valve body holder having the injection port into which one end of the capillary is fitted, and a second valve body holder having a communication port into which the other end of the capillary is fitted; a connecting tube that communicates and connects the first and second valve body holders separately from the capillary tube; and a connecting tube that is pressed by the capillary tube to open the valve when the capillary tube is fitted into the injection port and the communication port. Normally closed first and second valve bodies are provided in each of the first and second valve body holders so as to communicate and connect the first and second valve body holders through a capillary tube. A blood component quantitative meter according to claim (1). (3) In the valve open state where the first and second valve bodies are pressed by the capillary tube fitted in the injection port and the communication port, respectively, the connection port for the connection pipe is completely or partially closed. A blood component quantitative meter according to claim (2). (4) The valve device includes a cylinder, a first check valve that opens toward the cylinder chamber, which is provided in a communication portion between the cylinder chamber and the injection port, and a flow path through which the carrier liquid flows through the cylinder chamber. Claim No. 1 is provided with a second check valve that opens on the carrier liquid flow path side and is provided in a communication portion with the carrier liquid flow path.
Blood component quantitative meter described in ).