JPS6130593A - Method of extracting saponin component from soybeam hypocotyl - Google Patents

Abstract

PURPOSE:To obtain a saponin component to provide a food good for health and nutrition, having emulsifying action, antioxidant action, pharmacological action, etc. inexpensively and simply, by using soybean hypocotyl as a raw material, extracting it with hot water. CONSTITUTION:1pts.wt. soybean hypocotyl is immersed in preferably 4-10pts.wt. hot water usually in a boiling state for usually 4-10min, to give a saponin substance.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention does not use soybean seeds which contain only 0.01 to 0.03% of saponin components, but only contain 3 to 5% of saponin components.
The present invention relates to a method for extracting saponin components from soybean hypocotyl, which makes it possible to obtain saponin components with high yield and in a simple manner using soybean hypocotyl containing soybean hypocotyl as a raw material.

Glycine max Merrill
It has been discovered that saponin components contained in soybean seeds have a wide range of effects, including emulsifying, antioxidant, and pharmacological effects, and various attempts have been made to extract and purify saponin components from soybean seeds. For example, soybeans are ground, defatted with n-hexane, heated and extracted with methanol, and methanol is removed from the extract by drying under reduced pressure to obtain a mixture of crude saponin substances. This crude saponin component is dissolved in a mixture of n-butanol:water = 1; the water and n-butanol layers are separated, the saponin component is dissolved in the n-butanol layer, and the n-butanol is removed under reduced pressure. A known method is to further refine the residue obtained by removing the residue in step 2.3 to obtain a soya saponin component.

However, with these methods, 0.01 to 0.0
In order to extract the saponin component, which only contains 3%, a large amount of solvent is required during degreasing and methanol extraction, making it extremely uneconomical. Conversely, there was a problem in that the manufacturing cost of the saponin component was extremely high.

Therefore, as a result of researching the distribution of saponin components in soybean seeds, the present inventors confirmed that 90% or more of saponin components are present in the soybean hypocotyl. On the other hand, the applicant has already completed and proposed a method for mechanically selecting and extracting only the hypocotyls from soybean seeds using an appropriate process (Special Li No. 191842/1984). When the total amount of saponin in the hypocotyl obtained by this method was measured, it was found to reach 3 to 5%. One specific analysis example is as follows.

Shiny saponin r -----------------・----
---1--
・−・−2.9% total luster saponin−−・・−・・
・−・・・−・・−・・・・・−・・・・−・・・
・4.3% isoflavones and isoflavone glycosides・−・−−−・−−−−−−−−・−1−−−−・−・
−・−・・−5.1% Through further research, the applicant has completed a high-yield extraction and purification method for saponin components using soybean hypocotyl as a raw material and a solvent such as hot ethanol. , we have already proposed this (patent application 1983).
-45152). However, since this method mainly uses an organic solvent, the extraction cost increases accordingly, and its handling is also difficult.

The present inventor discovered that the saponin component in soybean hypocotyls can be easily extracted with hot water, and by using water that is convenient to handle and inexpensive, the present inventors have found that it is economical We have completed a method for extracting saponin components from soybean hypocotyl that can obtain saponin components at high yields.

. The gist of the present invention is to provide a saponin component from soybean hypocotyl, which is characterized in that soybean hypocotyl is used as a raw material, and this is treated with hot water to extract saponin-based substances in the hot water. It depends on the extraction method.

The soybean hypocotyl (germ) is obtained by the above-mentioned proposed method, which includes a sorting process to remove foreign substances from the raw whole soybeans, a heating process to make the soybeans soft, An auxiliary dehulling process to create cracks in soybeans, a dehulling process to peel the soybeans, a wind-selection process to remove the skin peeled off in the peeling process, and half-split cotyledons from the wind-selected soybean mixture. The sieving process for separating the soybean and the hypocotyl consists of a step, and according to this method, whole soybeans are almost completely separated into cotyledons, hypocotyls, and skins.

In this way, a raw material consisting almost 100% only of hypocotyls can be obtained. This hypocotyl raw material may be subjected to hot water treatment as it is, but it may also be subjected to treatments such as crushing or compressing to make it more susceptible to hot water extraction.

In the hot water treatment conditions, the ratio of soybean hypocotyl to water is not particularly limited as long as the soybean hypocotyl is immersed in water, but it is preferably 1 part by weight of soybean hypocotyl. Against 4~
10 parts by weight, or about 1:5 (weight ratio), is sufficient.

If the amount of water is too large, the extract will be diluted with saponin components, which will require post-processing such as concentration.If the amount of water is too small, the extract will be rich in saponin components, but A problem arises in that the extraction efficiency decreases as the concentration of the concentrated extract reaches an equilibrium state at a high concentration.

The treatment time of the hot water treatment is also not particularly limited, but it may be about 4 to 10 minutes, and a treatment time of about 5 minutes is sufficient. If the processing time is too short, the extraction will not be sufficient, and even if the extraction is carried out for a long time, the extraction amount will not increase much, but it will simply take more time and the efficiency will deteriorate.

There are no particular limitations on the temperature of the hot water, as long as it can extract the saponin component in the soybean hypocotyl. It is sufficient if the temperature is 50 degrees Celsius or higher, and it is possible to perform the treatment at a temperature of 50 degrees Celsius or lower if time is taken, but from the viewpoint of treatment efficiency, treatment at too low a temperature is not preferable. Usually, it is convenient to use boiling water because temperature control is easy.

The obtained extract of soybean saponin components has an astringent and astringent taste, but it can be drunk as it is, or it is very easy to drink by adding it to a drink or juice, and it has health effects and nutritional benefits. It makes an excellent beverage.

Moreover, if the extract is used as it is instead of water when preparing solid foods, it becomes possible to easily obtain foods that are excellent in terms of health and nutrition.

Examples of such foods include kutsky, cheese, kamaboko, and ozone.

On the other hand, the obtained extract may be purified by filtration or the like, and its concentration can of course be changed as appropriate by means of concentration, dilution, etc. Furthermore, it goes without saying that the extract can be subjected to freeze-drying treatment to form a solid powder, if desired.

As described above, according to the present invention, soybean saponin components can be extracted with high yield, inexpensively, and easily using soybean hypocotyl as a raw material, and the resulting extract can be used to improve health, Beverages and foods with excellent nutrition can be easily prepared.

real lid tama The present invention will be specifically described below with reference to Examples.

Example 1 5 kg of water was added to 1 kg of soybean hypocotyl, and the mixture was boiled in a boiling pot for about 5 minutes. Then, it was separated into the broth and the steamed soybean hypocotyl. The resulting broth was found to have a very acrid and astringent taste just by putting it in the mouth, indicating that most of the saponin components were extracted into the broth.

In addition, raw soybean hypocotyl has an acrid and astringent taste that makes it difficult to chew in the mouth, but soybean hypocotyl that has been steamed has less acridness and astringency. However, if the soybean hypocotyl was steamed only once, the remaining soybean saponin components remained in the soybean hypocotyl, so when 5 kg of water was added to the soybean hypocotyl that had only been steamed once and the same hot water treatment was performed, it separated. While the broth had an acrid and astringent taste, the two-component steamed soybean hypocotyl no longer had an astringent and astringent taste. Considering this fact, in actual hydrothermal treatment, in order to increase the extraction efficiency of soybean hypocotyl, two-component steaming is used to completely extract soybean saponin components.
It is economical to then dry the steamed soybean hypocotyl and use it as food or feed.

Foods containing saponin components could be easily obtained by adding and mixing this broth, ie, extract, with other foods. When we knead flour with this broth, add sugar and other seasonings, and bake it as kutski, we get all the acrid flavor.
I was able to make a kutsky that didn't taste like it.Also, when I filtered this broth and mixed it with orange juice, I was able to make a delicious drink that didn't taste harsh and didn't spoil the taste of orange juice.

Patent applicant: Pelican Co., Ltd. Procedural amendment July 8, 1985

Claims (3)

[Claims] (1) A method for extracting saponin components from soybean hypocotyl, which comprises using soybean hypocotyl as a raw material, treating it with hot water, and extracting saponin-based substances into the hot water. (2) A beverage characterized by adding saponin component-containing water obtained by extracting saponin components from soybean hypocotyls through hot water treatment. (3) A food product prepared by cooking using saponin-containing water obtained by extracting saponin components from soybean hypocotyls through hot water treatment.