JPH05271087A - Method for processing soybean hypocotyl, processed soybean hypocotyl and its utilization - Google Patents

Abstract

PURPOSE:To inexpensively and readily obtain the soybean hypocotyl processed product capable of wholly utilizing the soybean hypocotyl massively by-produced in a skin-removing process for the production of soybean processed beverages or foods and useful for improving and preventing hepatic function disorders and for maintaining health. CONSTITUTION:The soybean hypocotyl is thermally treated preferably at >=115 deg.C. Concretely, for example, the soybean hypocotyl is boiled in boiling water for a long time or closed in a heated pressure container and subsequently subjected to a wet thermal treatment with steam. When the soybean hypocotyl is thermally treated, the coexistence of an edible organic acid such as acetic acid or citric acid is preferable for the improvement in the hepatic function disorder-inhibiting effect.

Description

Detailed Description of the Invention

[0001]

[Field of Industrial Application] The present invention improves the liver function of humans or livestock using soybean hypocotyl that has been by-produced in a large amount in the molting step of processed foods and soybeans that are made from soybeans and has been discarded until now. The present invention relates to a method for processing soybean hypocotyl and a method for using soybean hypocotyl which can be effectively used not only for prevention but also for health.

[0002]

2. Description of the Related Art Conventionally, soybean hypocotyl has been produced as a by-product in a large amount in the molting process in manufacturing various foods and drinks made from soybeans such as soymilk, but it has been discarded as something that has no value until now. Was there. On the other hand, JP-A 64-683
18, JP-A-2-200640, JP-A-3
-31296 discloses that saponins contained in each part of legumes and other plants are known to be effective in improving or preventing liver dysfunction, and the saponin component is extracted with an organic solvent. It is known that the extract is subjected to various purification steps to isolate the active ingredient and use this as an agent for improving or preventing liver dysfunction. Therefore, following this, an attempt has been made to extract saponin from soybean hypocotyl with an organic solvent and give the purified saponin to mice to test the improvement and prevention of liver dysfunction.

[0003]

As described above, the amount of soybean hypocotyls discarded from the soybean processing industry that manufactures soymilk and other foods and drinks from the soybeans as a raw material as described above is nationwide. It is a huge loss from an industrial point of view, which is extremely uneconomical, and also causes environmental pollution problems that pollute rivers due to its disposal. .. According to the above-mentioned conventional technique, saponin is extracted from soybean hypocotyl with an organic solvent, further subjected to many purification steps for the extract, and purified, which is used as a therapeutic or preventive agent for liver dysfunction, A large amount of labor and an increase in manufacturing cost are required for the purification, and therefore, the product is not suitable for practical use by mass production and is an expensive product, while the residue that occupies most of the soybean hypocotyl after the extraction treatment is a so-called dreg. Since it is disposed of in the same manner as in the past, not only the amount thereof reaches an extremely huge amount, but also the problem of environmental pollution is not solved. In summary, soybean hypocotyl contains not only saponin, which is effective in improving and preventing liver dysfunction, but also contains a large amount of proteins, fats, sugars, minerals, vitamins B and E, and further anticholesterol and antilipemic blood. ,
Antioxidant, lecithin effective for intestinal action, etc., fiber, since it contains various useful functional substances such as oligosaccharides, not only its saponin component, but effective use of all of these useful components, It will bring enormous industrial benefits, and it will solve the huge economic loss and environmental pollution problem by conventional disposal. In view of this point, as described above, the saponin is extracted from the soybean hypocotyl with a solvent, and the target saponin is isolated from the extract through a number of purification steps. This is a big problem because various useful substances are melted away, and the discarded residue is not so-called slag but various useful substances are discarded. Therefore, the inventor crushed the by-produced soybean hypocotyl as it is and fed it to livestock, which caused indigestion and diarrhea, which caused a problem on the contrary. It is presumed that the reason for this is that not only the digestibility is poor because the feed is raw, but also digestion of proteins is hindered by harmful substances such as trypsin inhibitor. Moreover, a crushed product obtained by crushing the whole soybean hypocotyl or a concentrated saponin extract obtained by eluting it with a solvent, the dried product and the crushed product of the residue were used as feeds, and each of them was a 6-week-old person with liver dysfunction. When the inhibitory effect was given to male ddy mice of No. 1 and tested by a conventional method, almost no inhibitory effect was observed in any of the feeds.

[0004]

Means for Solving the Problems The present invention solves the above problems, makes the whole soybean hypocotyl by-produced available, and has an effect of suppressing liver dysfunction by an easy and inexpensive processing means. The present invention also provides a method for processing processed soybean hypocotyl effective as a health food for humans and livestock, and is characterized in that soybean hypocotyl is heat-treated.

[0005]

By heat-treating the soybean hypocotyl by-produced in this way, it becomes harmless for humans and livestock, and digestibility is improved, and the inhibitory effect on the prevention of liver dysfunction is exerted. A processed soybean hypocotyl, in which the whole hypocotyl can be used as a health food, is easily and inexpensively obtained.

When the soybean hypocotyl is heat-treated in the presence of an edible organic acid, the effect of suppressing liver dysfunction is further improved.

The temperature of the heat treatment in this case is about 100.degree.
Or higher, preferably 115 ° C. or higher. As a result, a remarkable improvement in prevention and improvement of hepatic dysfunction and a processed product which is harmless and is surely digestible are brought about. According to the present invention, the processed soybean hypocotyl thus obtained is used singly or as an additive for the prevention of liver dysfunction or health maintenance of humans or livestock, and the effective utilization of the whole soybean hypocotyl is achieved. Achieved, the enormous loss due to the conventional disposal and the problem of environmental pollution accompanying it are solved, and on the contrary, it brings a very great benefit to the industrial economy.

[0008]

EXAMPLES Next, examples of the present invention will be described in detail. Conventionally, in the production of processed foods and drinks using soybean as a raw material, the soybean hypocotyl produced as a by-product was discarded, but the present invention improves the liver function of the whole by simple processing means as described below. Not only for prevention, but also for reforming into a food that can be effectively used as a health food, eliminating the enormous economic loss nationwide that was discarded without the above-mentioned conventional utility value, and to convert it into a useful substance. At the same time, the problem of environmental pollution of rivers due to such conventional disposal can be eliminated. That is, a large amount of soybean hypocotyl, which is a byproduct of the soybean hulling process, that is, soybean hypocotyl mass, is obtained by removing a part or all of the cotyledon part fine grains contained in the soybean hypocotyl. Processed soybean hypocotyl which has an inhibitory effect on liver dysfunction, which is suitable for edible foods with improved digestibility by heating at a high temperature of at least about 100 ° C, preferably at 115 ° C or higher for a desired time It can be modified into trout and, in addition to proteins, sugars and other nutritional components contained in the processed soybean hypocotyl, it also effectively acts on anticholesterol, antioxidant, and other physiological functions such as circulatory organs. With various functional substances, it can be used as a so-called health food, which is advantageous.

In this case, as will be made clear by the following comparison test, it should be noted that the by-produced raw soybean hypocotyl is
Despite containing saponins and isoflavonoids, which have been considered to have inhibitory effects on liver dysfunction, most of them are effective in raw soybean hypocotyl or its extract and its residue. However, when the heat treatment of the present invention at a high temperature is performed, the suppressing effect is clearly recognized.
It was found that the effect is remarkably increased at 5 ° C or higher. The reason for this is not clear at present, but it is speculated that the high-temperature heat treatment of the soybean hypocotyl may produce a new substance having an inhibitory effect on liver dysfunction.

The high-temperature heat treatment in the present invention means that the soybean hypocotyl mass (hereinafter simply referred to as soybean hypocotyl) is cooked for a long time with boiling water, or sealed in a heating and pressurizing container, and steamed for about 11 minutes.
10 to 3 at a temperature of 5 ° C or higher, preferably 115 ° C or higher
It is a wet heat treatment in about 0 minutes, or a dry heat treatment at the above-mentioned high temperature in a roasting machine or an autoclave. In order to inactivate harmful substances such as triplysin inhibitor, heat treatment at 115 to 150 ° C is preferable. Thus, the heat treatment of soybean hypocotyl improves the digestibility due to the thermal alteration of its protein, etc.
It can inactivate enzymes that interfere with digestion, such as trypsin inhibitors, and transforms into harmless edible soybean hypocotyls. Thus, although the reason is not clear, the dry matter of the extract extracted with water or an organic solvent or the residue thereof is added to the granular soybean or as shown in the specific examples below. In any of the above conditions, it exerts an inhibitory effect on liver dysfunction. Therefore, as in the past, paying attention to the appearance of saponins contained in legumes or plants, it is troublesome to isolate saponins from the extract, and it is necessary to carry out purification work which increases manufacturing costs and loss of other active ingredients. It is possible to eliminate the inconvenience that occurs and to mass-produce inexpensive products for improving and preventing liver dysfunction with a significantly simpler manufacturing process than before.

In the heat treatment of the present invention, an appropriate amount of at least one edible organic acid such as acetic acid or citric acid is added to the soybean hypocotyl to be processed, and the soybean hypocotyl is processed as described above in the coexistence thereof. When heat-treated, the processed soybean hypocotyl having a further improved effect of suppressing liver damage can be obtained.

Since the processed soybean hypocotyl thus obtained by the heat treatment contains various active ingredients and nutritional ingredients as described above, it can be used alone in humans and livestock (including pets). , Or when added to foods and drinks and samples, not only prevent liver dysfunction improvement, but at the same time provide nutritional supplements of functional substances such as proteins, qualities, vitamins and minerals to maintain health. Helps maintain good health by preventing adult diseases.

The processed soybean hypocotyl of the present invention is not limited to itself, but an extract obtained by extracting the processed soybean hypocotyl with water or ethanol, hydrous ethanol, or any other desired organic solvent, and the residual solid matter (so-called residue). It was found that both of them both had an inhibitory effect on liver dysfunction. From this point as well, the active ingredient that suppresses liver dysfunction is considered to be due to another substance that is not necessarily related to saponin, and it is speculated that such a substance was produced by the high temperature heat treatment of the present invention. ..

Therefore, according to the present invention, the processed soybean hypocotyl is used in the form of its particles, or in the form of a crushed powder, or its extract or its concentrate, Alternatively, it is used in the state of its dried product, or its residue or its pulverized product. The powder may be pressure-molded into a specific size and used as a molded product such as granules or tablets, or in the form of capsules,
The extract or concentrate may also be in the form of capsules.

The processed soybean hypocotyl of the present invention may be supplied directly to humans or livestock alone in the above-mentioned arbitrary use state, or may be added at any stage in the production of food or drink, or It is added to the compounded feed to prepare as a food or drink for health use or a sample for health use, and is supplied indirectly.

As described above, the processed soybean hypocotyl of the present invention is easy to manufacture and can be mass-produced at low cost, and is useful not only for improving and preventing liver dysfunction, but also for being utilized as various functional foods at the same time.

Next, more specific examples of the present invention will be described in detail together with comparative examples. Example 1 150 g of soybean hypocotyl to be processed was placed in an autoclave, 10 times amount of water was added thereto, and the mixture was immersed overnight to swell the soybean hypocotyl. Next, the swollen soybean hypocotyl was heated for 30 minutes in boiling water in the autoclave. Next,
The processed soybean hypocotyl thus obtained was cooled and then placed in a vacuum dryer and dried. Next, the dried product was pulverized with a pulverizer to obtain 98 g of powder. This was designated as Sample 1. Example 2 200 g of soybean hypocotyl to be processed was placed in an autoclave, 600 ml of water was added thereto, and the mixture was immersed overnight to swell the soybean hypocotyl. Next, the swollen soybean hypocotyl was heat-treated at 110 ° C. for 10 minutes in the autoclave. Next, the processed soybean hypocotyl thus obtained was cooled and then dried in a vacuum dryer. Next, the dried product is crushed with a crusher to obtain powder 1
74 g was obtained. This was designated as Sample 2. Example 3 200 g of soybean hypocotyl to be processed was placed in an autoclave, 10 times the amount of water was added thereto, and the mixture was immersed overnight to swell the soybean hypocotyl. Next, the swollen soybean hypocotyl was treated in the autoclave at 115 ° C. for 10 minutes. Next, the processed soybean hypocotyl thus obtained was drained and then dried in a vacuum dryer. Next, the dried product is pulverized with a pulverizer to obtain powder 10
4 g was obtained. This was designated as Sample 3. Example 4 200 g of soybean hypocotyl to be processed was placed in an autoclave, 10 times amount of water was added thereto, and the mixture was immersed overnight to swell the soybean hypocotyl. Next, 2.7 ml of acetic acid was added, and the mixture was heat-treated in the autoclave at 115 ° C. for 10 minutes. Next, the processed soybean hypocotyl thus obtained was drained and then dried in a vacuum dryer. Next, the dried product was crushed with a crusher to obtain 124 g of powder. This was designated as Sample 4. Example 5 300 g of soybean hypocotyl to be processed was placed in an autoclave, 10 times amount of water was added thereto, and the mixture was immersed overnight to swell the soybean hypocotyl. Then, in the autoclave 12
Heat treatment was performed at 1 ° C. for 15 minutes. Next, the processed soybean hypocotyl thus obtained was drained and then dried in a vacuum dryer. The dried product was pulverized with a pulverizer to obtain 182 g of powder. This was designated as Sample 5. Example 6 400 g of soybean hypocotyl to be processed was placed in an autoclave, 10 times the amount of water was added thereto, and the mixture was immersed overnight to swell the soybean hypocotyl. Next, after adding 5.4 ml of acetic acid, heat treatment was performed at 121 ° C. for 15 minutes in the autoclave. Next, after draining the processed soybean hypocotyl thus obtained,
It was dried in a vacuum dryer. Next, the dried product was pulverized with a pulverizer to obtain 248 g of powder. This was designated as Sample 6. Example 7 300 g of soybean hypocotyls were placed in a dry heater and heat-treated at 180 ° C. for 30 minutes. That is, it was roasted and heated. Next, after cooling the processed soybean hypocotyl thus obtained, crushed with a crusher,
268 g of powder was obtained. This was designated as Sample 7. Example 8 To 600 g of the processed soybean hypocotyl obtained by the heat treatment of Example 7, 4-fold amount of 70% ethanol was added, and the mixture was heated at 80 ° C. for 3 hours for extraction treatment. Then, this was filtered to obtain an extract.
Furthermore, the same extraction process was performed twice. The extract thus obtained for 3 times was concentrated under reduced pressure to obtain 179 g of a dried product. This was designated as Sample 8. On the other hand, the residue of the processed soybean hypocotyl separated from the extract by the above filtration is dried under reduced pressure,
419 g of dried product was obtained. This was designated as Sample 9. Control Example 600 g of by-produced soybean hypocotyl was extracted three times in the same manner as in Example 8, and the extract thus obtained for three times was concentrated under reduced pressure to obtain 159 g of a dried product. This was designated as Sample 10. On the other hand, the residue separated from the extract by filtration was dried under reduced pressure to obtain 395 g of a dried product. This was used as feed 11.

Next, in order to examine the inhibitory effect on liver dysfunction, these samples 1 to 11 were subjected to animal experiments as follows. Experimental Example 6-week-old male ddy mice were preliminarily fed with a normal diet (CE-2 manufactured by CLEA Japan, Inc.) for 1 week, and then 10 each group was used.
A group of animals in which 10% of each of the above feeds was added to a normal diet and a group of which no feed was added were provided as animals, and each group was bred for 1 week. After 1 week of breeding, 2% (W / W) carbon tetrachloride suspended in olive oil was intraperitoneally administered at 3.3 ml / Kg, and after 24 hours, a thoracotomy was performed and blood was collected from the heart and centrifuged. Separation of serum in serum, and GOT and GPT activity in serum are POP ・ TO
It was measured by the OS method (transaminase measurement kit manufactured by Wako Pure Chemical Industries, Ltd.), and the inhibition rate was calculated from the following mathematical formula.

[0019]

[Equation 1]

In the above formula, the normal group refers to a group that is fed only with a normal diet and does not receive carbon tetrachloride, and the control group refers to a group that is fed with only a normal diet and then administered with carbon tetrachloride, and a sample group. Each group means a group to which carbon tetrachloride was administered after breeding with a normal diet to which each sample was added. The results are as shown in Table 1 below.

[0021]

[Table 1]

Sample 1, Sample 2, ..., Sample 11 in the column of group species in Table 1 were raised with a sample group in which Sample 1 was added to a normal diet and a sample in which Sample 2 was added to a normal diet, respectively. Sample group, ... Means sample groups fed with samples obtained by adding sample 11 to normal food, respectively.

As is clear from Table 1 above, when the samples 10 and 11 from the untreated soybean hypocotyl of the control example were given, there was no inhibitory effect, but the heat treatment temperature was about 100 ° C.
It can be seen that, when each of Samples 1 to 9 of the examples, which are the processed soybean hypocotyls of the present invention treated with the above high temperature, is given, an inhibitory effect on liver dysfunction is exhibited. When the heat treatment temperature is 115 ° C. or higher, the suppression rate is improved,
When the heating temperature is higher, the inhibition rate increases, and particularly when heat treatment is performed in the presence of an acid, or when dry heating is performed,
It was confirmed that the suppression rate was remarkably improved. This means that the heat treatment of soybean hypocotyl not only suppresses saponin, but also some substance that exerts its suppressive effect on liver dysfunction, and a large amount is generated as the heat treatment temperature rises. Seemed to do, and discovered a new, interesting and important fact. In general, heating at 115 ° C may be used, but heating at 120 ° C or higher is particularly preferable, and 15
It was confirmed that heating at 0 ° C. or higher inactivates harmful substances in soybean hypocotyl, improves digestibility, and further improves and prevents liver dysfunction.

[0024]

As described above, according to the present invention, by heating the by-product raw soybean hypocotyl, it is easily and inexpensively converted into a useful substance having a liver function-suppressing effect and has good digestibility. Supply to humans and livestock to prevent liver dysfunction,
Not only does it bring about an improving effect, but since it also supplies functional substances along with the various nutritional components it contains, it can be used as a so-called health food and can be added to foods and drinks as a health food or health feed. While it has the effect of increasing its product value, it eliminates the enormous loss of soybean hypocotyls that had previously been disposed of, and on the contrary, can be used economically effectively, which is a great benefit to the industrial economy. In addition, it brings about great effects such as solving the problem of environmental pollution that has been conventionally associated with disposal.

Claims (5)

[Claims] 1. A method for processing soybean hypocotyl, which comprises heat-treating soybean hypocotyl. 2. A method for processing soybean hypocotyl, which comprises heat-treating soybean hypocotyl in the presence of an edible organic acid. 3. The method for processing soybean hypocotyl according to claim 1, wherein the temperature of the heat treatment is about 100 ° C. or higher, preferably about 115 ° C. or higher. 4. A processed soybean hypocotyl which has been heat-treated. 5. A method of use, wherein the whole processed soybean hypocotyl is used alone or added to foods and drinks or feed to be used for preventing liver function improvement in humans or livestock and for other health purposes.