JPS61181961A - Data processor for chromatograph - Google Patents

Abstract

PURPOSE:To enable accurate identification of components separated with a chromatograph, by providing an automatic correction means which performs an automatic correction using an identification time range from an identification table to accomplish a highly accurate identification. CONSTITUTION:An analog signal from a chromatograph is converted into a digital signal with an A-D converter 1 to be brought into a central processor 3 via a peripheral connector 2 and deformed into a data in a specified form set by a control command unit 5 by an instruction from a control instruction storing unit 5 to be stored into a signal data storing unit 6. On the other hand, when this signal data reaches a fixed amount, a painting is performed through an output device 7 and by repetition of this operation, a chromatoagraph is obtained. When conducting an identification, a reference sample is analyzed in this manner and the retention time of reference components is measured to prepare a table for identification, which is stored into an identification table storing unit 9. This enables accurate identification of components separated with the chromtograph thereby achieving a higher reliability.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to a data processing device for chromatography, and more particularly, to a data processing device for chromatography that is suitable for highly accurate identification of separated components in chromatography. It is something.

[Background of the invention]

Conventionally, the identification of components separated by chromatography was performed manually for each component. For example, in the case of identification, an identification table was manually set for each peak. Since the identification was performed manually in this way, the retention time deviation of each component was input in advance as a constant. 'Therefore, when analyzing actual samples, the components often did not match the identification table.

[Purpose of the invention]

The present invention has been made in view of the above, and its object is to provide a data analysis device for gas chromatography that can accurately identify the No. 9 component separated by chromatography.

[Summary of the invention]

The present invention is characterized by: an identification table creator that creates an identification table; an identification table storage that stores the identification table; and an automatic table corrector that specifies automatic correction of the identification table. and a correction table storage device that stores the retention time of each component when measuring the standard sample, and the central processing unit stores the retention time of each component when measuring the standard sample. The present invention has an automatic correction means that calculates the identification time width of a component, stores it in a correction table, and automatically corrects the identification time width as the identification time width of the fixed table, and is configured to perform highly accurate identification. It is in.

[Embodiments of the invention]

The present invention will be described in detail below with reference to the embodiments shown in FIGS. 1, 4, and 5, and FIGS. 2 to 3.

FIG. 1 is a block diagram showing an embodiment of a chromatographic data processing apparatus of the present invention. In FIG. 1, an analog signal from a chromatograph (gas chromatograph or liquid chromatograph) is converted from an analog signal to a digital signal by an A-D converter IK, and then passed through a peripheral device connector 2 to a central processor 3. be taken in. and,
The data is set by the control command unit 5 in response to a command from the control command storage unit 4, is transformed into data in a predetermined format, and is stored in the signal data storage unit 6. On the other hand, when this signal data reaches a certain amount, it is printed by the output device 7. This operation is repeated to obtain a chromatogram as shown in FIG. 2(a).

When performing identification, first analyze the standard sample in this way, measure the standard component glue tension time, create an identification table by the identification table creator 8, and store it in the identification table storage 9. .

FIGS. 3(a) and 3(b) are nine views showing an example of an identification table. In FIG. 3(a), A is the separated component number, C is the retention time of the separated component, C is the identification width, 2 is the response factor of the separated component, and E is the component group of the separated component. By the way, in the present invention, FIG. 3(a)
The key to creating the identification table shown in Figure 1 is to have an identification width (tolerable width) for each component. In chromatography,
Even if the analysis conditions are always kept constant, there are factors in which the outflow of each component is not always constant, so a certain range of identification is absolutely necessary, and - this range of identification must be changed during the analysis. There are also similar analyses. Be friends,
Each component has an identification width, and K is set so that all components can be identified. By the way, when analyzing a sample with a large number of components, for example, a petroleum-based sample, 100 to 300 components are separated. Inputting the identification width for each peak requires a great deal of effort. Therefore, identify C in Figure 3(a)! When inputting ALLOW, the first component (e.g.
When ALLOW of 几T=1.00) in Figure 3 is set to 1, the next component (RT=2.0O) (7) ALLOW is also 1.
This method saves you the trouble of inputting information. And the component of RT=3.00 has ALI and OW of 2
When set, ALLO of the next BT=400 component
Copy W as 2 as well. In this case, the fixation table may be created using any other suitable method depending on the analytical component.

For general analysis, use this table to create a calibration curve and obtain analysis results for actual samples.

In other words, the analysis process consists of a) analysis for creating a calibration curve, (2) analysis of actual samples, and qualitative analysis to obtain quantitative results. is important.

When creating a calibration curve, perform multiple analyzes to improve accuracy and take the average value. At this time, automatic correction of the fixing table is designated by the automatic table corrector 10 shown in FIG. As a result, the retention time of each component is stored in the correction table storage 11 shown in FIG. 1 when measuring the standard sample for creating a calibration curve. In the example of Figure 2(b), 3
Assume that the retention time of the component A in the figure is as follows.

1st time T=4.10 minutes 2nd time T=3.80 minutes 3rd time BT=4.34 minutes Therefore, the average value is (4,10+&80+4.34
) / 3 = 4.08 minutes. As a result, the retention time BT of the identification table for the components of A is shown in Figure 3 (
The time is automatically changed from 4.00 minutes in a) to 4.08 minutes in FIG. 3(b). The identification width ALLOW is also automatically corrected as follows. That is, 1 average value - minimum value 1
Alternatively, the identification width is corrected to the larger of 1 average value - maximum [1]. In the above example, 14.08-3.801=
0.28, +4.08-4.341=0.26
Of these, 0.28 is the identification width, and ALLOW is shown in Figure 3 (a
) is automatically rewritten from 2 in Figure 3 to 0.28 in ). In short, from the table in Figure 3(a) to the table in Figure 3(a)
The table will be automatically changed to b). As a result, in the analysis of the actual sample, as shown in FIG. 2(C), if the fourth peak exists within 4.08 minutes±0.28 minutes, it is identified as component A.

The above processing in the central processor 3 of FIG. 1 will be explained using the flowcharts shown in FIGS. 4 and 5.

Figure 4 is a flowchart for the process of creating an identification table.
In step 51, it is determined whether the table creation is a new one or a modification. If it is a new table creation, the retention time RT is manually operated in step 52, and the identification width AL is changed in step 53.
A LOW input operation is performed, and in step 54, the above numerical value is copied and stored as ALLOW for the next component. In step 55, the response factor FA of the separated component is
Perform CTOR input operation, and in step 56, input the component salt NA.
ME is input, steps 52 to 56 are repeated for the number of components, and the treatment is completed. In addition, in the case of correction in step 51, input the retention time RT of the component to be corrected in step 57, and input the identification width of the component to be corrected in step 58. Perform input operations, do not copy.

Figure 5 is a flowchart of the process for quantitative calculation, step 7.
In step 1, the results of n-time analysis of the standard sample for creating a calibration curve are stored, and in step 72, the average value of the retention time BT of each component is calculated, and the results are stored in a correction table. In step 73, the deviation width of the retention time RT of each component is calculated, and the maximum value of the deviation width is calculated from the data stored in step 71 as ALL in the identification table.
The value is OW. The results are stored in the correction table as in step 72. In step 74, the average value of retention times RT in the correction table is replaced with the identification table. In step 75, the retention time identification @ALLOW is replaced from the correction table with the identification table.

In step 76, quantitative calculations of each component are performed with reference to the identification table automatically rewritten as described above for the actual sample.

In addition, in FIG. 1, 12 is a control condition display device, and 13 is a parameter storage device.

[Effects of the Invention] As explained above, according to the present invention, the identification width of the next component can be automatically set when creating an identification table.
It is possible to prevent a decrease in the number of inputs and input errors, and it is also possible to calculate the deviation width of the identification width of each component when measuring a standard sample, so it is possible to accurately identify the ninth component separated by chromatography. This has the effect of increasing reliability.

[Brief explanation of drawings]

FIG. 1 is a block diagram showing an embodiment of the chromatographic data processing device of the present invention, FIG. 2 is a diagram showing an analysis example, FIG. 3 is a diagram showing an example of an identification table, and FIG. 4 is a diagram showing an example of an identification table. FIG. 1 is a flowchart showing an embodiment of identification table creation processing in the central processor, and FIG. 5 is a flowchart showing an embodiment of quantitative calculation processing. 1... A-D converter, 2... Peripheral device connector, 3...
...Central processor, 4...Control 4 command storage unit, 5...Control command unit, 6...Signal data storage unit, 7...Output device, 8...Fixing table creator, 9...Fixing table storage device, 10...Table automatic corrector, 11...
- Correction table storage unit, 12... control condition display unit,
13... 1st mouth 2nd part of the rosette (,oo 2.oo 3-oo 4oo minute 4・b
8 (g)

Claims (1)

[Claims] 1. An A-D converter that converts an analog signal from a chromatograph into a digital signal, and an A-D converter that takes in the digital signal and transforms it into data set by a control command device and stores it in a signal data storage device. In a chromatographic data processing device comprising a central processor for storing signal data and an output device for printing when signal data reaches a predetermined amount, there is provided an identification table creator for creating an identification table, and an identification table generator for creating an identification table. an identification table storage device for storing an identification table; an automatic table correction device for specifying automatic correction of the identification table; and a correction table storage device for storing retention times of each component when measuring a standard sample; The central processor is
When identifying the separated components of the chromatograph using the identification table, the identification time width of each component by measurement of the standard sample is calculated and stored in a correction table, and the identification time width is used for the identification table. A data processing device for chromatography, characterized in that it is equipped with an automatic correction means for automatically correcting the identification time width of a table, and is configured to perform highly accurate identification. 2. The automatic correction means corrects the retention time of the identification table prepared in advance according to the analytical component to the average value of the retention time of each component during the measurement of the standard sample, and the identification time width is determined based on the retention time. Claim 1, which is configured to correct the identification time width to the larger of the difference between the average value and the minimum value or the average value and the maximum value.
The chromatographic data processing device described in Section 1.