JPS61164158A - Composition for inspection of protein - Google Patents

Abstract

PURPOSE:To obtain a compsn. for easy detection of protein by incorporating a specific acidic dye, solvent, water and, if desired, pH adjusting agent into said compsn. CONSTITUTION:The compsn. contg. the acidic dye, solvent, water and if desired, pH adjusting agent is brought into contact (by coating, spraying, dipping, etc.) with the stain desired to be inspected and the discrimination by coloration is made possible if protein exists. The acidic dye is designated by the dye name and dye number in cosmetic tar dye classification; for example, amaranth (red No.2), erythrosine (red No.3), etc. are used. The compounding ration thereof is made 0.05-10wt%, more preferably about 0.3-2wt%. For example, alcohol, glycol, etc. are used for the solvent in order to improve cold resistance and wettability and to permit the easy discrimination if fats co-exist. The compounding ratio thereof is made about <=99%, more preferably about 30-70wt%. The water is preferably demineralized ion and refined water. The pH adjusting agent is compounded with the compsn. when needed in order to improve the sensitivity and for example, acetic acid, lactic acid, etc. are used to adjust the pH to about 2-12, more preferably to about 3-7.

Description

[Detailed description of the invention] (Industrial application field) The present invention relates to a composition for protein testing.

(Prior Art and its Problems) Even if you think that tableware or medical equipment (syringes, etc.) have been thoroughly washed, stains often remain. When cleaning with an automatic washing machine, it appears on the surface that it has been completely cleaned, but in reality, a considerable amount of residue is observed. Such a situation is unfavorable from a hygienic and pathological point of view.

Therefore, if there is a method to easily determine the presence or absence of these contaminants, we would use this method to periodically check for contamination, and if the contamination has not yet been removed, we would either redo the cleaning process or clean it again during the cleaning process. Methods and cleaning agents can be considered. There are already preparations that detect only fat.

(Objective of the Invention) An object of the present invention is to provide a composition for easily detecting proteins.

(Means for Solving the Problems) That is, the present invention relates to a protein testing composition containing an acid dye, a solvent, water, and optionally a pH adjuster.

Examples of acidic dyes used in the present invention include amaranth (red No. 2), erythrosin (red No. 3), newcoccin (red No. 102), eosin YS (red 10
No. 3 (1)), Eosin YsK (Red No. 103 (2)
)), Phloxin B (Red No. 104 (1)), 70 Xin BK (Red No. 104 (2)), Rose Bengal (Red No. 105 (1)), Acid Red (Red No. 10
6), Fast Green FCF (Green No. 3), Brilliant Blue FcF (Blue No. 1), Indigo Carmine (
Blue No. 2), Lysol Rubin B (Red No. 201), Fast Acid Magenta (Red No. 227), Pyranine Conch (Green No. 204), Light Green SF Yellow (Green No. 20)
5), Patent Blue NA (Blue No. 202), Patent Blue CA (Blue No. 203), Alphazurin FG (
Blue No. 205), Resorcin Brown (Brown No. 201)
, Violamine R (Red No. 401), Bon/-3R (Red No. 502), Bonso R (Red No. 503), Bonso S
X (Red No. 504), Fast Red S (Red No. 506), Naphthol Green B (Green No. 401), Guineag! J-:/B (Green No. 402), Arizurol Purple (Purple No. 401), Naphthol Blue Black (Black No. 4)
No. 01) and Amido Black 10B1 acidic fuchsin,
Examples include aniline blue WS and mixtures thereof.

Preferably, amaranth (red No. 2), erythrosine (
Red No. 3), New Coccin (Red No. 102), Phloxin B (Red No. 104 (1)), Phloxin BK (Red No. 104 (2)), Acid Red (Red No. 106), Fast Green FCF ( Green No. 3), Brilliant Blue F'CF (Blue No. 1), Indigo Carmine (
Blue No. 2). In terms of skin irritation and safety, it is most preferable to use cosmetic tar pigments and food additive pigments, such as New Coccin (Red No. 102), Acid Red (Red No. 106), or a mixture thereof. preferable.

It is thought that the acidic raw material becomes negatively (-) charged in an aqueous solution and binds to positively (+) charged biological constituents (proteins containing many amino acids and the like having NHt groups) to cause staining.

The amount of acidic dye blended is 0.05 to 10% by weight, preferably about 0.1 to 5% by weight, particularly preferably about 0.2 to 3%,
Most preferably about 0.3-2% by weight. If the amount is less than 0.05% by weight, it is difficult to determine the protein, and although it may be added in an amount exceeding 10% by weight, there will be virtually no difference in the determination ability, resulting in increased cost and loss of safety.

The solvent is added to improve cold resistance and wettability, and to prevent the composition liquid of the present invention from being repelled by the fat and making it difficult to judge when fat and protein coexist as stains. Any solvent may be used as long as it achieves this purpose, but from the viewpoint of safety, solvents such as alcohols, glycols, polyhydric alcohols, and glycol ethers are preferred. Examples of alcohols include methanol, ethanol, n-propanol, isopropanol, butanol and denatured alcohol. Further, examples of glycol include ethylene glycol, propylene glycol, and the like. Examples of glycol ethers include diethylene glycol monoethyl ether, methyl triglycol, isopropyl glycol, propylpropylene glycol, and examples of water-soluble polyhydric alcohols include glycerin and sorbitol. Preferably, ethanol, denatured alcohol, n-propanol, isopropanol, glycerin,
Sorbitol or mixtures thereof may be mentioned. In particular, ethanol, denatured ethanol, or a mixture thereof is preferred from the viewpoint of safety, odor, fat solubility, etc.

The blending amount of the solvent is about 99% or less, preferably about 20 to 80%.
% by weight, particularly preferably about 30-70% by weight.

Any type of water may be used, such as tap water, deionized water, purified water (distilled water), etc., but impurities such as hardness (normally, tap water in Osaka or Tokyo etc. contains 30 to 50 ppm Ca).
The use of deionized or purified water is preferred, considering the presence of CO3 (containing CO3) and chlorine, etc.

A pH adjuster is added to improve sensitivity, and may not be necessary depending on the type of solvent or dye. In general, any substance that can lower the pH and is water-soluble may be used.

For example, organic acids such as acetic acid, lactic acid, butyric acid, malic acid, citric acid, succinic acid, gluconic acid, and glutamic acid; inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, and nitric acid; or mixtures thereof. Considering odor and safety during actual use, it is preferable to use acids for food additives such as lactic acid, butyric acid, malic acid, citric acid, succinic acid, glutamic acid, phosphoric acid, or mixtures thereof.

1) Adjust the pH of the composition to approximately 2 by adding an H adjuster.
~12, preferably about 3-10, particularly preferably about 3-7
Adjust to. Within this range, color development is easy and quick.

Although the composition of the present invention may be used in any form, it is generally convenient and easy to use it in a spray type. The spray may be gas-filled or non-gas-filled. The type of gas in the spray is not particularly limited, and examples thereof include fluorocarbons, DMESLPG, and the like.

(Actions and Effects of the Invention) The protein detection composition of the present invention comes into contact with the stain to be inspected (by coating, spraying, dipping, etc.) and if proteins are present, the stain is colored, which enables determination. In particular, if the substance is heated at the time of determination, it will be easier to determine the effect. However, if both starch and protein are present, both will be colored, so in this case, if you perform an iodine-starch reaction after checking the protein, only the starch will be colored, so the presence of both and The presence or absence of starch can be determined.

The present invention will be explained in more detail with reference to Examples.

Examples and Comparative Examples Comparative Example 1 A composition for protein testing was prepared by mixing the following components; Base Minutes Weight (9) Ninhydrin 0.3 Lysphosphoric Acid (Anhydrous)
Appropriate amount Ethanol (99.5%) 99 Deionized water Remaining amount The pH of the obtained composition was 4.45 (21° C.) The stability of the protein was fair.

Examples 1 to 36 A composition for protein testing was prepared in the same manner as in Comparative Example 1 using the components listed below.

Example 1 Amido Black I OB 0.5 Citric acid (anhydrous) Appropriate amount of methanol
50 Deionized water Remaining amount 1 carp λ Weight (g) Bonso SX (
Red No. 504) ■Citric acid (anhydrous)
Appropriate amount of methanol 50 Deionized water Remaining amount Example 3 Weight (g) Ponceau 3R (
Red No. 502)! Citric acid (anhydrous)
Appropriate amount of methanol 50 Deionized water Remaining amount Example 4 Ash-minute Weight (g) Erythrosin (Red No. 3) 0.2 Deionized water Remaining amount Example 5 Flash-minute Weight (9) Erythrosin (Red No. 3) ■Methanol
50 Deionized water Remaining amount Example 6 Disaster Weight (9) Erythrosin (Red No. 3) 1 Citric acid (anhydrous)
Appropriate amount Ethanol (99.5%) 30 Deionized water Remaining amount Example 7 Area - Outside Weight (g) Erythrosin (Red No. 3) 1 Citric acid (Anhydrous)
Appropriate amount of ethanol (99.5%) 50
Deionized water Remaining amount Example 8 - Minutes Weight (9) New Coccin (Red No. 102) 0.05 Citric acid (Anhydrous)
Appropriate amount of ethanol (99.5%)
Remaining amount deionized water 0.99 Example 9 Ingredients Weight (g) New Coccin (Red No. 102) 0.1 Citric acid (anhydrous) Appropriate amount Ethanol (99.5%)
50 Deionized water Remaining amount Example IO Bottom-Ki Weight (g) New Coccin (Red No. 102) 0.3 Citric acid (anhydrous)
Appropriate amount of ethanol (99.5%) 5
0 Deionized water Remaining amount Example 11 Bottom-minute Weight (I?) New Coccin (Red No. 102) ■ Ethanol (99.5%)
49 Deionized water Remaining amount 12-Example I2 / min Weight (g) New Coccin (Red No. 102) ■ Citric acid (anhydrous)
Appropriate amount Ethanol (99.5%) 49 Deionized water Remaining amount Example 13 Weight (g) New Coccin (Red No. 102) 1 Citric acid (anhydrous)
Appropriate amount Ethanol (99.5%) 10 Deionized water Remaining amount Example 14 Weight (9) New coccin (red No. 102) I citric acid (anhydrous)
Appropriate amount of ethanol (99.5%) 30 Deionized water Residue Example 15 New Coccin (Red No. 102) 1 Citric acid (anhydrous) Appropriate amount of ethanol (99.5%) 50 Deionized water Remaining amount Example 16 Layout Weight (li) Acid Red (Red No. 106) 1 Citric acid (anhydrous)
Appropriate amount of ethanol (99.5%) 49 Deionized water Remaining amount Example 17 Bottom-minute Weight (g) Acid Red (Red No. 106) 10 Citric acid (anhydrous) Appropriate amount of ethanol (9
9.5%) 20 Deionized water Remaining amount Example I8 Bottom - Public Weight (9) Acid Red (Red No. 106) ■ Dilute hydrochloric acid
Appropriate amount Propylene glycol 30 Deionized water Remaining amount Example 19 Broiled weight (9) Acid Red (Red No. 106) ■Diethylene glycol
30 Monoethyl ether Deionized water Remaining amount Example 20 Yield - External Weight (2) Acid Red (Red No. 106) Lactic acid Appropriate amount Glycerin 30 Deionized water Remaining amount Example 21 Yi - External Weight (9) Acid Red (Red No. 106) No.) Citric acid (anhydrous) Appropriate amount of ethanol (9
9.5%) 10 Deionized water Remaining amount Example 22 / min Weight (f Acid Red (Red No. 106) l Citric acid (anhydrous)
Appropriate amount Ethanol (99.5%) 30 Deionized water Remaining amount ° Example 23 Weight (g) Acid Red (Red No. 106) 1 Citric acid (anhydrous)
Appropriate amount Ethanol (99.5%) 50 Deionized water Remaining amount Example 24 Bottom - min Weight (g) Amaranth (
Red No. 2) l Citric acid (anhydrous)
Appropriate amount of ethanol (99.5%) 20 Deionized water Remaining amount Example 25 Ingredients Weight (9) Brilliant Blue FCF 1 (Blue No. 1) Citric acid (anhydrous) Appropriate amount of ethanol (
99.5%) 49 Deionized water Remaining amount Example 26 Weight (9) Indigo carmine (Blue No. 2) 1 Citric acid (anhydrous)
Appropriate amount Ethanol (99.5%) 49 Deionized water Remaining amount Example 27 Weight (9) Eosin YS
1 (Red No. 103 (1)) Citric acid (anhydrous) Appropriate amount of ethanol (9
9.5%) 49 Deionized water Residual amount Example 28 Yield Minutes Weight (9) Eosin YS
K 1 (Red No. 103 (2)) Citric acid (anhydrous) Appropriate amount of ethanol (9
9.5%) 49 Deionized water Remaining amount Example 29 Weight (9) Alphazurin FG 1 (Blue No. 205) Citric acid (anhydrous) Appropriate amount Ethanol (9
9.5%) 49 Deionized water Remaining amount Example 30 Weight (9) Arizurol Purple 2 (Purple No. 401) Citric acid (anhydrous) Appropriate amount Ethanol (9
9.5%) 49 Deionized water Remaining amount Example 31 (Red No. 104 (1)) Citric acid (anhydrous) Appropriate amount of ethanol (
99.5%) 49 Deionized water Residual amount Example 32 Abdomen Weight (fF) Acidic Tsuksin l Ethanol (99.5%) 49 Deionized water Remaining amount Example 33 Weight (fF) Aniline Blue WS 1 ethanol (99,5%)
49 Example 34 (Green No. 3) Ethanol (99.5%) 49 Deionized water Remaining amount Example 35 Abdomen - Minutes Weight (g) Newcoccin (Red No. 102) 0.5 Acid Dred (Red No. 106
No.) 0.5 Citric acid (anhydrous) Appropriate amount Ethanol (99.5%) 49 Deionized water Remaining amount Example 36 Weight (9) Acid Red (Red No. 106) ■Propylene glycol
25 Ethanol (99.5%) 25 Deionized water The detectability of residual protein was good in Examples 1, 9, and 24, except for Comparative Example 1 and Example 8, and good or good in all other Examples. It was either very good.

Example 6, Example 7, Examples 13 to 15, Example 21
From Example 23 and Example 3-5, the compatibility with gas was measured using DME. All of these were good.

Claims (1)

[Scope of Claims] 1. A protein testing composition containing an acid dye, a solvent, water, and optionally a pH adjuster. 2. Acidic dyes include amaranth (Red No. 2), erythrosin (Red No. 3), newcoccin (Red No. 102), phloxine B (red No. 104 (1)), phloxine BK (
Red No. 104 (2)), Acid Red (Red No. 106)
2. The composition according to claim 1, which is Fast Green FCF (Green No. 3), Brilliant Blue FCF (Blue No. 1), Indigo Carmine (Blue No. 2) or a mixture thereof. 3. The composition according to item 2, wherein the acid dye is erythrosin (Red No. 3), New Coccin (Red No. 102), acid red (Red No. 106), or a mixture thereof. 4. The composition according to any one of items 1 to 3, wherein the amount of the acid dye is about 0.05 to 10% by weight. 5. Fourth, the amount of acid dye is about 0.1 to 5% by weight
Compositions as described in Section. 6. No. 5 in which the amount of acid dye is about 0.2 to 3% by weight
Compositions as described in Section. 7. The composition according to item 6, wherein the amount of the acid dye is 0.3 to 2% by weight. 8. The composition according to item 1, wherein the solvent is ethanol, denatured alcohol, n-propanol, isopropanol, glycerin, sorbitol, or a mixture thereof. 9. The composition according to item 8, wherein the solvent is ethanol, denatured ethanol, or a mixture thereof. 10. The composition according to item 1, wherein the amount of the solvent is about 20 to 80% by weight. 11. No. 10 in which the amount of solvent is about 30 to 70% by weight
Compositions as described in Section. 12, pH adjuster is lactic acid, butyric acid, malic acid, citric acid,
2. The composition according to claim 1, which is succinic acid, glutamic acid, phosphoric acid or a mixture thereof. 13. The composition according to item 1, wherein the composition for protein testing has a pH of about 2 to 12. 14. The composition according to item 13, wherein the composition for protein testing has a pH of about 3 to 10. 15. The first protein test composition has a pH of about 3 to 7.
Composition according to item 4. 16. The composition according to item 1, wherein the protein testing composition is a spray protein testing composition.