JPS6114798B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a method for producing a corresponding halogenated epoxide from a halogenated olefin using microorganisms. Prior Art Epoxide compounds are widely used as raw materials or intermediates for the production of various organic chemical products including synthetic resins, surfactants, medicines, and agricultural chemicals. Methods for producing epoxide using microorganisms include Nocardia spp., Mycobacterium spp.
Genus Methylococcus, Genus Methylosinus,
Pseudomonas spp., Corynebacterium spp.
It is known that microorganisms belonging to the genus Methylobacterium and Candida produce epoxides from linear olefins, but there have been no reports on the production of halogenated epoxides. Purpose of the Invention The present invention was made based on the knowledge that among the above-mentioned microorganisms, epoxide-producing bacteria belonging to the genus Nocardia can also produce corresponding halogenated epoxides from halogenated olefins. An object of the present invention is to provide a method for producing a corresponding halogenated epoxide from a halogenated olefin by using microorganisms having the ability to produce epoxides. The present invention will be explained in detail below. Structure and Effects of the Invention The structural feature of the present invention is that a microorganism belonging to the genus Nocardia that has an ability to produce epoxide is allowed to act on a halogenated olefin under aerobic conditions, and the produced halogenated epoxide is separated and collected. There is a particular thing. The microorganism used in the present invention belongs to the genus Nocardia, and can be exemplified by Nocardia corallina. This bacterium is stored at the Institute of Microbial Technology, Agency of Industrial Science and Technology with the accession number FERA-P-4094, dated June 15, 1978, and its mycological properties are published in Details are given in Publication No. 40. In the present invention, halogenated olefin (hereinafter referred to as raw material olefin) used as a reaction substrate for producing epoxide using the above-mentioned microorganisms
Examples include halogenated straight-chain or branched olefins (olefins in which the double bond is in the α, β, or γ position from any terminal carbon atom), such as allyl chloride, allyl bromide, allyl iodide, 3-chloro-1-butene, 3-chloro-2methyl-propene, 1-chloro-2-butene, 2-fluoropropene, 3.
Examples include 3,3-trifluoro-propene. In the present invention, these raw material olefins are used alone or as a mixture of two or more, or as a mixture with a hydrocarbon other than olefin, such as a saturated hydrocarbon or an aromatic hydrocarbon, as a reaction substrate. In the present invention, in order to cause the microorganism to act on the raw material olefin, for example, (a) a method in which the raw material olefin is brought into contact with bacterial cells obtained by culturing and propagating the microorganism in advance under aerobic conditions to cause a reaction; b) A method of culturing the above-mentioned microorganisms under aerobic conditions in a nutrient medium consisting of raw material olefin or raw material olefin and other carbon sources, a nitrogen source, inorganic salts, and, if necessary, a growth promoting substance. applicable. In the method of (a) above, in which the raw material olefin is brought into contact with the growing bacterial cells and reacted, first, as a carbon source, carbohydrates such as glucose, sucrose, molasses, starch hydrolyzate, cellulose hydrolyzate, hydrocarbons such as propane, butane, etc. Dodecane, tetradecane, and other substances with high bacterial growth effects such as acetic acid are used, and nitrogen sources such as ammonium chloride, ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, aqueous ammonia, amino acids, and other assimilable organic nitrogen compounds are used. , potassium phosphate,
Sodium phosphate, magnesium sulfate, manganese sulfate, ferrous sulfate, ferric chloride, calcium chloride,
The medium is supplemented with inorganic salts such as manganese chloride and, if necessary, growth-promoting substances such as vitamins, yeast extract, and corn staple liquor.
An inoculum of epoxide-producing bacteria belonging to the genus Nocardia is inoculated and cultured under aerobic conditions to multiply bacterial cells. The raw material olefin and air, oxygen, or oxygen-enriched gas are added directly to the bacterial cell culture obtained in this way, or to a suspension of bacterial cells isolated from the culture, or to a suspension of bacterial cells isolated from the culture. A reaction is caused by supplying an oxygen-containing gas such as The reaction takes place in the pH range of 5 to 9, preferably 6 to 8.
1-6 at a temperature of 20-50℃, preferably 25-45℃
Do it for days. The reaction is usually carried out under normal pressure, but the productivity of epoxide can also be improved by carrying out the reaction under increased pressure. The activity of the reaction between the bacterial cells and the raw material olefin can be maintained or increased by appropriately adding the carbon source, nitrogen source, and other components used for bacterial growth during the reaction. The reaction can be carried out either batchwise or continuously. In the case of a batch reaction method, the raw material olefin can be supplied in its entirety at the start of the reaction, or can be supplied continuously or intermittently during the reaction. The epoxide produced in the reaction solution by the above reaction is separated and collected by applying known techniques such as phase separation, extraction, and distillation. Next, the culture method (b) is a method in which raw material olefin is added during bacterial growth in the method (a) to produce epoxide in one step. The culture conditions (PH, temperature, pressure, etc.), the culture method, and the separation and collection of the generated epoxide are based on the reaction conditions described in (a) above.
Reaction schemes and separation and collection methods can be similarly used. The epoxide obtained by the present invention can be used in the various conventionally known uses mentioned above. The present invention will be explained in more detail with reference to Examples below. Example 1 Nocardia corallina B-276 (Agency of Industrial Science and Technology, Institute of Microbial Technology, deposit number FERM-P-4094)
Three platinum loops were mixed with NBG medium (manufactured by Oxoid, "Lavrenko" powder, 10 g of code L29, 10 g of bacteriological peptone, code L37, 10 g of glucose, 5 g of sodium chloride, and deionized water was added to make 1000 ml, and 1N caustic soda aqueous solution was added. The mixture was inoculated into a 500 ml Sakaguchi flask containing 100 ml of a liquid medium (liquid medium) which had been heated to 7.5 in an autoclave at 120°C for 15 minutes, and cultured with shaking (150 times/min) at 30°C for 48 hours. The bacterial cells produced by this culture were washed once with a 0.01 molar phosphate buffer (PH7.5), and then once with the reaction medium shown below.
A bacterial suspension was prepared by resuspending the cells in the following reaction medium to a dry bacterial cell concentration of 3.8 mg/ml. Reaction medium K ₂ HPO ₄ 1.74 g MgSO ₄・7H ₂ O 1.50 g FeSO ₄・7H ₂ O 50 mg Deionized water 1 PH 8.0 (PH was adjusted with 2N sulfuric acid aqueous solution) 20 ml of the bacterial suspension, 1 ml of glucose ( A 100 mg/ml aqueous solution) was placed in a 500 ml Sakaguchi flask and the flask was tightly stoppered, and each raw material olefin shown in Table 1 was added through an injection port equipped with a rubber gasket. In addition,
In the case of raw material olefin that is liquid at room temperature, 100 μl of the material was pressurized into the above-mentioned flask, and in the case of gaseous material at room temperature, 40 ml (at room temperature and under normal pressure) of the raw material was injected into the flask. then 30
After 24 hours of culturing with reciprocating shaking at 150 times/min at ℃, 1 ml of the gas phase above the reaction solution in the flask and 1μ
The remaining reaction solution was extracted with 50 ml of diethyl ether, and 1 μm of the ether layer was sampled and analyzed. PorapakQ (Waters) was used for analysis.
A Hitachi model 163 ionizing flame gas chromatograph equipped with a glass column of 3 mm in inner diameter and 2 m in length packed with 80 to 100 mesh (manufactured by Associates, Inc.) was used. The product produced by the reaction was measured with a gas chromatograph, and the retention time and mass spectrum measured with a mass spectrometer connected to the gas chromatograph were compared with the retention time and mass spectrum of a standard sample. It was confirmed that it was a corresponding epoxide. Table 1 shows the types of raw material olefins and the corresponding amounts of epoxide produced. The amount of epoxide produced was determined by gas chromatography and expressed as the concentration in the reaction solution. 【table】

Claims (1)

[Scope of Claims] 1. A method for producing epoxide, which comprises allowing a microorganism belonging to the genus Nocardia to produce epoxide to act on a halogenated olefin under aerobic conditions, and separating and collecting the produced halogenated epoxide. . 2. The manufacturing method according to claim 1, wherein the microorganism having epoxide-producing ability belonging to the genus Nocardia is Nocardia corallina.