JPS61126031A - コロニ−形成刺激因子の製造法 - Google Patents
コロニ−形成刺激因子の製造法Info
- Publication number
- JPS61126031A JPS61126031A JP59248204A JP24820484A JPS61126031A JP S61126031 A JPS61126031 A JP S61126031A JP 59248204 A JP59248204 A JP 59248204A JP 24820484 A JP24820484 A JP 24820484A JP S61126031 A JPS61126031 A JP S61126031A
- Authority
- JP
- Japan
- Prior art keywords
- inhibitor
- medium
- cell
- culture
- stimulating factor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims description 20
- 102000007644 Colony-Stimulating Factors Human genes 0.000 title abstract 3
- 108010071942 Colony-Stimulating Factors Proteins 0.000 title abstract 3
- 239000003112 inhibitor Substances 0.000 claims abstract description 16
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims abstract description 15
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 241001465754 Metazoa Species 0.000 claims abstract description 7
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 6
- 244000068988 Glycine max Species 0.000 claims abstract description 6
- 229940122618 Trypsin inhibitor Drugs 0.000 claims abstract description 4
- 101710162629 Trypsin inhibitor Proteins 0.000 claims abstract description 4
- 239000002753 trypsin inhibitor Substances 0.000 claims abstract description 4
- 108700018667 Streptomyces subtilisin inhibitor Proteins 0.000 claims abstract description 3
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 claims abstract 2
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 claims abstract 2
- 230000005757 colony formation Effects 0.000 claims description 4
- 230000004936 stimulating effect Effects 0.000 claims description 3
- 108010039627 Aprotinin Proteins 0.000 claims description 2
- 108010043846 ovoinhibitor Proteins 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 2
- 244000005700 microbiome Species 0.000 claims 2
- 102100033312 Alpha-2-macroglobulin Human genes 0.000 claims 1
- 108010015078 Pregnancy-Associated alpha 2-Macroglobulins Proteins 0.000 claims 1
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- 108010069594 plasminostreptin Proteins 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 11
- 206010038389 Renal cancer Diseases 0.000 abstract description 3
- 238000000746 purification Methods 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 241000272473 Aquila chrysaetos Species 0.000 abstract 1
- 208000006265 Renal cell carcinoma Diseases 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 201000010174 renal carcinoma Diseases 0.000 abstract 1
- 235000013311 vegetables Nutrition 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 description 22
- 210000004027 cell Anatomy 0.000 description 19
- 239000002609 medium Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 10
- 210000000349 chromosome Anatomy 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 5
- 239000013587 production medium Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000012980 RPMI-1640 medium Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 239000000654 additive Substances 0.000 description 3
- 210000002798 bone marrow cell Anatomy 0.000 description 3
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 229920000609 methyl cellulose Polymers 0.000 description 3
- 239000001923 methylcellulose Substances 0.000 description 3
- 235000010981 methylcellulose Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000003760 tallow Substances 0.000 description 3
- 208000008839 Kidney Neoplasms Diseases 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000003714 granulocyte Anatomy 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 201000010982 kidney cancer Diseases 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000003643 myeloid progenitor cell Anatomy 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000045232 Canavalia ensiformis Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000010617 Phaseolus lunatus Nutrition 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 108010001122 alpha(2)-microglobulin Proteins 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 201000002364 leukopenia Diseases 0.000 description 1
- 231100001022 leukopenia Toxicity 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59248204A JPS61126031A (ja) | 1984-11-26 | 1984-11-26 | コロニ−形成刺激因子の製造法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59248204A JPS61126031A (ja) | 1984-11-26 | 1984-11-26 | コロニ−形成刺激因子の製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61126031A true JPS61126031A (ja) | 1986-06-13 |
| JPS6238325B2 JPS6238325B2 (OSRAM) | 1987-08-17 |
Family
ID=17174738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59248204A Granted JPS61126031A (ja) | 1984-11-26 | 1984-11-26 | コロニ−形成刺激因子の製造法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61126031A (OSRAM) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6360010U (OSRAM) * | 1986-10-07 | 1988-04-21 | ||
| JP2002515454A (ja) * | 1998-05-20 | 2002-05-28 | エラスムス ユニフェルシテイト ロッテルダム | 炎症または掻痒の予防または治療方法および手段 |
-
1984
- 1984-11-26 JP JP59248204A patent/JPS61126031A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6360010U (OSRAM) * | 1986-10-07 | 1988-04-21 | ||
| JP2002515454A (ja) * | 1998-05-20 | 2002-05-28 | エラスムス ユニフェルシテイト ロッテルダム | 炎症または掻痒の予防または治療方法および手段 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6238325B2 (OSRAM) | 1987-08-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |