JPS61116658A - Thrombocyte agglutinating capacity measuring apparatus - Google Patents

Abstract

PURPOSE:To achieve a high accuracy and a higher speed, by executing the computation of a agglutinating capacity pattern with a microprocessor (MP) to display the results thereof. CONSTITUTION:Sample solutions (a) and (a') of the same quantity are injected into both transparent containers 16 and 16' while an agglutimation deriving substance (ADP). Then, the temperature control of thermostatic units 1 and 1' is done with a temperature control circuit 8 while the speed control done with a motor control circuit 9. Upon the start of measurement, output voltages of photodiodes 5 and 5' are amplified 7 respectively and digitized with an A/D converter in an interface 10 to be transferred to an MP11. Then, the ADP is added into containers 16 and 16' from a pepete 6 in a different amount. After the injection of the ADP, as a manual start switch 6a is turned ON to start a clock in an MP11, an agglutinating capacity pattern is shown on a liquid crystal display 14. With a printer 15, the type of agglutinating capacity patterns varying with time and the final agglutinating capacity pattern is printed.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Use This invention relates to a platelet 6j concentration measuring device in the field of clinical testing.

BACKGROUND OF THE INVENTION Platelet aggregation tests, which are closely related to the hemostatic function of IIIL fluid, are becoming increasingly important as their relationship with thrombosis and various other diseases has been elucidated.

However, since the mechanism of platelet aggregation is complex, there is a problem that the test includes unstable elements and it is quite difficult to identify the test results, and there is no established method for the K''/11 set. .

In recent years, compared to the conventional judgment method, the pattern ``1:+1 constant method'' based on two kinds of fourths, which has a relatively high stability of 1IllI constant 11, has been developed and has been evaluated as γ positive ( rTHROMBO5I
s REst! ARCHVO1, 11j p, 453
Pergamon Press Ltd, in G
reatBritain) e This pattern 4JI standard method with two concentrations is platelet rich blood 51P RP (PIateleL Rich Pla
ADP (Adenosi)
By preparing two types of samples with different added amounts of ne di Phosphate, measuring and recording the temporal changes in the amount of transmitted light of both samples in parallel, and visually comparing the recorded aggregation ability patterns, several types of This is a judgment method that categorizes

Problems to be Solved by the Invention However, the above conventional pattern determination method using two types of density has the following problems.

■ Due to the visual comparison, there is a possibility that the subjectivity of the observer inevitably enters, and this has the drawback of causing errors in the judgment results.

11
was inviting.

■ Whales were observed visually, and checking against the identification conditions was also a manual process, which resulted in long measurement times and low efficiency.

OBJECTS OF THE INVENTION It is an object of the present invention to solve the above-mentioned problems and to provide a platelet aggregation ability measuring device that can measure platelet aggregation ability with extremely high accuracy and speed.

Means for Solving the Problems The technical means (structure of the invention) taken by this invention to solve the above problems are as follows.

That is, the platelet aggregation ability measuring device of the present invention includes a first transparent storage container for storing a first sample solution containing platelet-rich plasma and adding an aggregation-promoting substance, and a first transparent storage container in the first transparent storage container. a first platelet aggregation ability detector comprising a first light emitter/receiver that irradiates a sample solution with a light beam and receives the transmitted light; and a platelet-rich plasma in the first sample solution.
[a second transparent storage container for a second sample solution containing platelet blood cells and to which an aggregation-promoting substance in an amount different from that of the aggregation-promoting substance in the sample solution of description 1 is added; a second platelet aggregation detector comprising a second light emitter/receiver that irradiates a second sample solution in a transparent storage container with a light beam and receives the +3 rays of light; A microprocessor that inputs the pincer detection signal and calculates the aggregation pattern from a combination of both detection signals, and a display that is connected to this microprocessor and displays data on the cardiac aggregation pattern as the result of the microprocessor's calculations. It is equipped with a device.

action According to the above configuration of the present invention, the following effects are achieved.

+a+ Since the calculation of the cohesiveness pattern is executed by a microprocessor, there is no room for subjectivity, and the judgment of the cohesiveness pattern can be performed at extremely high speed and with high precision. That is, there are no errors caused by individual differences that occur in the case of visual observation, and highly reliable measurement of the concentration pattern can be performed automatically.

(bl) Since the display device displays the agglomeration pattern data as the result of calculation by the microprocessor, the result of calculation by the microprocessor can be quickly and clearly understood.

Embodiment An embodiment of the present invention will be described with reference to FIGS. 1 to 4. Fig. 1 is a schematic configuration diagram including a block diagram of the entire Aki small aggregation ability measuring device, Fig. 2 is a schematic flowchart of its operation, and Fig. 3 (A) to (G) are various agglutination ability patterns. The graphs in FIGS. 4(A) to 4(D) are diagrams showing aggregation ability patterns actually displayed as data.

In FIG. 1, A is the first platelet aggregation detector, A'
is the second platelet aggregation detector, and both platelet aggregation detectors Δ and A' have the same configuration. That is, both platelet a-intensive detection 'JHA'.

A' is 5, the main body 1a, la' and the lid body 1b, respectively.

lb', a constant temperature unit 1.1' consisting of a main body la,
Transparent storage container i formed in la'! S16. +6' storage space 17, 17', electric heaters 2, 2' provided at the bottom of the main bodies 1a and 1a', and the main body 1a.

Light emitting diode 4゜4' as a floodlight installed at la'
, a photodiode 5.5' as a light receiver, and a stirrer 18.1 placed in a transparent container 16.16'.
Stirrer motor 3 that rotates 8' by magnetic coupling
．． 3'.

The re-emitting diode 4.4' is connected to a common amplifier circuit 7, both electric heaters 2, 2' are connected to a common temperature control circuit 8, and both stirrer motors 3.3' are connected to a common motor control circuit. Connected to circuit 9.

6 stores the aggregation-promoting substance ADP, and a transparent storage container 16.
This is a pipette for adding to the sample solution (platelet-rich plasma PRP) a, a' in the pipette 6'.
A manual start switch 6a is attached.

Amplification circuit 7. i degree control circuit 8. The motor control circuit 9 and the manual start switch 6a are each connected to an interface 10, the interface IO is connected to a microprocessor (CPU) 11, and the microprocessor 11 is connected to a ROM (read only memory) 12.
and a RAM (random access memory) 13.

Microprocessor 11 is connected to liquid crystal display 14 and printer 15 via interface 10 . The liquid crystal display 14 and the blink 15 are an example of a "display device" in the structure of the invention, but the liquid crystal display 14 alone or the printer 15 alone may be used.

Further, the display is not limited to a liquid crystal display, and may include a VDT (video display terminal) such as a CRT (cathode ray tube).

The liquid crystal display 14 graphically displays the temporal change in the aggregation ability pattern calculated by the microprocessor 11. The printer 15 prints a graph of the temporal change in the cohesive ability pattern and also prints the type of the cohesive ability pattern.

FIG. 2 shows an overview of the operation of the microprocessor 11.

(2) For the sample solution a in which the aggregation promoter ADP has a low concentration, a low concentration signal due to the low aggregation rate is input.

(2) Input a low concentration signal to sample data about sample solution a every 2 seconds and perform A/D conversion.

■ Sequentially supply power and select the maximum agglomeration value.

■ Divide the agglutination value after 10 minutes by the maximum a concentration value.

■ In parallel with ■, divide the maximum agglomeration value by the full scale.

■ Aggregation ability pattern [A] under the conditions in Table 1.

CB) and (C).

Steps 2' to 2' similar to 1 to 2 are performed for the sample solution a' having a high concentration of the aggregation-promoting substance ADP.

■Low concentration sample volume? f(A), distinguished by ■, ■' for Pi, a and high concentration sample solution a'.

CB), (c)l, f (A), r
From the combination of nl and [C] 1, distinguish between the final aggregation ability patterns [1] to [7] in Table 2.

max in Tables 1 and 2 represents the maximum aggregation rate.

Table 1 (blank below) Table 2 Regarding platelet aggregation ability, final pattern (1) is decreased, final pattern [2] to [3] is normal, final pattern [4]
~ [5] is slightly yr, progressive, final pattern (6) is advanced,
They each judge that the final pattern [7] is extremely advanced.

The graphs of the temporal changes of the final patterns (1) to [7] are shown in Figures 3 (A) to (B). The horizontal axis represents time and the vertical axis represents the condensation rate. Among the characteristic curves, the upper curve represents a low concentration sample solution a, and the lower curve represents a high concentration sample solution a'.

Note that the more the aggregation progresses, the more the transmitted light increases.

The measurement procedure using the platelet aggregation ability measuring device having the following configuration will be explained.

(1) Pour the same amount of sample solutions a and a' into both transparent storage containers 16 and 16', and place them in the storage space 17°17.
', and insert the lids 1b and Ib'.

On the other hand, the aggregation promoter ADP is injected into the pipette 6.

(2) The temperature control circuit 8 controls the temperature of the constant temperature unit 1.1' by the electric heater 2. The speed is controlled so that the rotation speeds of the two are the same.

When the measurement is started, the output voltages of the photodiodes 5 and 5' are each amplified by the amplifier circuit 7, digitized by the A/D converter in the interface 10, and transmitted to the microprocessor 11.

(3) Trial 11'4 melted by Starte 18.18'tFi, a.

While a' is IIMI↑, DP is injected and added to the aggregation-inducing substance from the pipette 6 into each transparent storage container 16.16'. , 0.5 μM for the first transparent storage container 16 (M is 4 degrees molar and 427/l).

) is added to the second transparent storage container 16', and 2 μM of aggregation promoter @lJ prize ADP is added to the second transparent storage container 16'.

Immediately after completing the injection of the aggregation promoter ADP into both sample solutions fia, a', turn on the manual start switch a. This starts the clock within the microprocessor 11.

(4) On the liquid crystal display 14, the horizontal axis displays the passage of time, and the vertical axis displays the optical output voltage input from the photodiode 5.degree. 5' via the amplifier circuit 7, that is, the aggregation ability pattern. At the same time, the printer 15 prints the temporally varying optical output voltage (cohesive power pattern) and the type of final cohesive power pattern.

FIG. 4 shows some examples of data actually obtained.

Figure (8) is a pattern ([8], r to 11 silk combination, and the high concentration max is 10% or more, so
The final pattern is [2].

Since Figure (B) is a combination of patterns ((A) and (B)l), the final pattern is [3].

Figure (C) is a combination of pattern ((Al, (C)l), and the max of low concentration is less than 15%, so the final pattern is (4).

Since diagram (D) is a combination of patterns ([C], [C]),! The Hiiragi pattern is (7).

In addition, the aggregated young substances added to platelet-rich PRP
The concentration of ADP is 0.5 to 111 M on the low concentration side, and about 2 to 5 μM on the high concentration side.

Effect of the invention According to this invention, there are the following effects.

Since the aggregation pattern is calculated by a microprocessor, there is no room for subjectivity, and the aggregation pattern can be determined at extremely high speed and with high precision. This method eliminates the errors caused by individual differences that occur in the case of conventional methods, and allows automatic determination of highly reliable aggregation ability patterns.

[bl Since the display device displays the agglomeration pattern data as the calculation results of the microprocessor, the results of one calculation by the microprocessor can be quickly and clearly understood.

[Brief explanation of the drawing]

Fig. 1 is a schematic configuration diagram including a block diagram of the entire platelet aggregation ability measuring device according to an embodiment of the present invention, Fig. 2 is a schematic flowchart of its operation, and Fig. 3 (A,) to (G) are graphs of various aggregation ability patterns, Fig. 4 (A) to (D)
is a display diagram of an aggregation ability pattern actually displayed as data. A...first platelet aggregation detector, A'...second platelet aggregation detector, a...first sample solution, a'.
...Second sample solution, 4...Light-emitting diode (first floodlight), 4'...Light-emitting diode (second floodlight)
, 5... Photodiode (first light receiver), 5'.
...Photodiode (second light receiver), 11...Microprocessor, 14...Liquid crystal display (display device), 15...Printer (display device) Fig. 1, Fig. 2. (A) (E) (B)
(F) (() (G) −PfFIWl (D) −F!IfIJll−@藺(A) CB)=1
-＠藺(C) (D)Figure 4

Claims (2)

[Claims] (1) A first transparent storage container for storing a first sample solution containing platelet-rich plasma and adding an aggregation-promoting substance, and a first sample solution in this first transparent storage container are irradiated with a light beam. a first platelet aggregation ability detector comprising a first light emitter/receiver that receives transmitted light; and a platelet-rich plasma containing the same amount of platelet-rich plasma as in the first sample solution; A light beam is irradiated onto a second transparent storage container for a second sample solution to which an amount of aggregation-initiating substance is added that is different from that of the aggregation-initiating substance in the second transparent storage container, and the second sample solution in this second transparent storage container. a second platelet aggregation ability detector consisting of a second light emitter/receiver that receives the transmitted light; and inputting the detection signals of both platelet aggregation ability detectors and calculating an aggregation ability pattern from a combination of both detection signals. A platelet aggregation ability measuring device comprising: a microprocessor that performs the following operations; and a display device that is connected to the microprocessor and displays aggregation pattern data as a calculation result of the microprocessor. (2) Platelet aggregation ability measurement according to claim (1), wherein the display device is a combination of a device that graphically displays temporal changes in the aggregation ability pattern and a device that displays the type of the aggregation ability pattern. Device.