JPH04552B2 - - Google Patents

Description

[Detailed description of the invention]

INDUSTRIAL APPLICATION FIELD This invention relates to a platelet aggregation ability measuring device in the field of clinical testing. PRIOR ART Platelet aggregation tests, which are closely related to the hemostatic function of blood, are becoming increasingly important as the relationship with thrombosis and various other diseases is elucidated. However, since the mechanism of platelet aggregation is complex, there are problems in that the test includes unstable elements and it is quite difficult to judge the test results, and there is no established method as a standard method. In recent years, a pattern determination method using two concentrations, which has relatively high measurement stability compared to conventional determination methods, has been developed and is attracting attention ("THROMBOSIS").
RESEARCH Vol, 11” p.453 Pergamon Press
Ltd. in Great Britain). In this pattern determination method using two concentrations, two samples are prepared by adding the aggregation-promoting substance ADP (Adenosine di Phosphate) to platelet rich plasma PRP (Platelet Rich Plasma) in different amounts. This is a judgment method that classifies into several types by measuring and recording temporal changes, that is, temporal changes in aggregation rate in parallel, and visually comparing the recorded aggregation patterns. The aggregation rate is defined as the intensity of light transmitted through platelet-poor plasma, where there is almost no scattering by platelets, as 100%.
The intensity of the transmitted light at each time is shown as a percentage, assuming that the intensity of the transmitted light of the cloudy platelet-rich plasma (in actual measurement, the intensity of the transmitted light of the sample solution immediately after adding the aggregation-inducing substance) is 0%. It is something. Problems to be Solved by the Invention However, the conventional pattern determination method based on blood concentration has the following problems. Due to the visual comparison, there is a possibility that the subjectivity of the observer inevitably enters, and this causes a defect that causes an error in the judgment result. The discrimination conditions (described in the above-mentioned documents) are extremely complex and wide-ranging, leading to situations in which, for example, the judgment results differ even among experts. Since it relies on visual observation and also requires manual labor to check against the discrimination conditions, it takes a long time to measure, resulting in low efficiency. Purpose of the invention The purpose of this invention is to solve the above problems,
It is an object of the present invention to provide a platelet aggregation ability measuring device that can perform platelet aggregation ability pattern determination with extremely high accuracy and speed. Means for Solving the Problems The technical means (structure of the invention) taken by this invention to solve the above problems are as follows. That is, the platelet aggregation ability measuring device of the present invention includes a first transparent storage container for storing a first sample solution containing platelet-rich plasma and adding a high concentration aggregation-inducing substance, and a first transparent storage container in the first transparent storage container. a first platelet aggregation ability detector comprising a first light emitter/receiver that irradiates a light beam onto a sample solution and receives the transmitted light beam to detect a temporal change in aggregation rate; a second transparent storage container for a second sample solution containing the same amount of platelet-rich plasma as the platelet-rich plasma and to which a low concentration of aggregation-inducing substance is added; and a second sample solution in the second transparent storage container. A second light emitting/receiving device that irradiates a light beam onto the target and receives the transmitted light beam to detect temporal changes in the aggregation rate.
inputting the detection signals of the platelet aggregation ability detector and the first and second platelet aggregation ability detectors, and calculating the temporal change in the aggregation rate based on the maximum aggregation rate or the aggregation rate after a predetermined time for both detection signals. a microprocessor that distinguishes two or more types of aggregation patterns, respectively, and determines the type of platelet aggregation ability pattern from a combination of the types of aggregation patterns of both detection signals; and a display device that displays the type of platelet aggregation ability pattern that is the determination result. Effects According to the above configuration of the present invention, there are the following effects. (a) inputting the detection signals of the first and second platelet aggregation detectors into the microprocessor;
Based on the maximum aggregation rate or the aggregation rate after a predetermined time for both detection signals, the aggregation pattern, which is a temporal change in the aggregation rate, is divided into two or more types, and platelet-rich plasma is determined from the combination of the types of aggregation patterns of both detection signals. Since the type of aggregation ability pattern is determined, there is no room for subjectivity and the determination of the platelet aggregation ability pattern can be performed at extremely high speed and with high accuracy. That is, there are no errors caused by individual differences that occur in the case of visual observation, and highly reliable aggregation ability patterns can be automatically measured. (b) Since the display device displays the type of platelet aggregation ability pattern as the determination result of the microprocessor, the determination result of the microprocessor can be known quickly and clearly. Embodiment An embodiment of the present invention will be described based on FIGS. 1 to 4. Fig. 1 is a schematic configuration diagram including a block diagram of the entire platelet aggregation ability measuring device, Fig. 2 is a schematic flowchart of its operation, and Fig. 3 shows A to A.
G is a graph of various aggregation ability patterns, Figure 4A~
D is a display diagram of an aggregation ability pattern actually displayed as data. In FIG. 1, A is a first platelet aggregation detector, A' is a second platelet aggregation detector, and both platelet aggregation detectors A and A' have the same configuration. That is, both the platelet aggregation detectors A and A' have main bodies 1a and 1a' and lid bodies 1b and 1b, respectively.
1b', a constant temperature unit 1, 1', and a main body 1.
Transparent storage containers 16, 16' formed in a, 1a'
storage spaces 17, 17', electric heaters 2, 2' provided at the bottom of the main bodies 1a, 1a',
A light emitting diode 4 as a floodlight provided at a′,
4', and a photodiode 5 as a photodetector.
5', and stirrer motors 3, 3' for rotating stirrers 18, 18' placed in transparent storage containers 16, 16' by magnetic coupling. A common amplifier circuit 7 for both light emitting diodes 4 and 4'
and both electric heaters 2,
2' is connected to a common temperature control circuit 8, and both stirrer motors 3, 3' are connected to a common motor control circuit 9. 6 stores the agglutinating substance ADP, and the sample solution (platelet-rich plasma) in the transparent storage container 16, 16'
PRP) is a pipette for adding to a, a′,
This pipette 6 is equipped with a manual start switch 6a. The amplifier circuit 7, temperature control circuit 8, motor control circuit 9, and manual start switch 6a are each connected to an interface 10, and the interface 10 is a microprocessor (CPU).
11, and the microprocessor 11
It is connected to a ROM (read-on memory) 12 and a RAM (random access memory) 13. The microprocessor 11 is connected to a liquid crystal display 14 and a printer 15 via an interface 10. The liquid crystal display 14 and the printer 15 are an example of a "display device" in the structure of the invention, but the liquid crystal display 14 alone or the printer 15 alone may be used. In addition, the display does not have to be limited to a liquid crystal display; it can also be a CRT (cathode ray tube), etc.
Includes all VDTs (Video Display Terminals). The liquid crystal display 14 graphically displays the temporal change in the aggregation rate calculated by the microprocessor 11. The printer 15 prints a graph of the temporal change in the aggregation rate and also prints the type of platelet aggregation ability pattern. FIG. 2 shows an outline of the operation of the microprocessor 11. For the sample solution a in which the aggregation promoter ADP has a low concentration, a low concentration signal due to the low aggregation rate is input. A low concentration signal is input, and data regarding sample solution a is sampled every 2 seconds and A/D converted. Select the maximum agglomeration value by sequential subtraction. Divide the agglutination value after 10 minutes by the maximum agglutination value. In parallel, the maximum agglomeration value is divided by the full scale. Under the conditions in Table 1, the agglomeration patterns are [A], [B],
[C] For the sample solution a' having a high concentration of the aggregation-promoting substance ADP, the same steps '-' are carried out. Low concentration sample solution a and high concentration sample solution
Regarding a′, {[A], [B], distinguished by ′
[C]}, {[A], [B], [C]} Platelet aggregation patterns in Table 2 [1] to [7]
Distinguish between max in Tables 1 and 2 represents the maximum aggregation rate.

【table】

【table】

[Table] Regarding platelet aggregation ability, platelet aggregation pattern [1] decreases, platelet aggregation pattern [2]~
[3] is normal, platelet aggregation pattern [4]~
[5] Slightly increased, platelet aggregation ability pattern [6]
is judged to be enhanced, and platelet aggregation pattern [7] is judged to be extremely enhanced. Graphs of each temporal change in aggregation rate corresponding to platelet aggregation patterns [1] to [7] are shown in Figure 3 A to G.
Shown below. The horizontal axis represents time and the vertical axis represents aggregation rate. Of the two characteristic curves, the upper curve represents the low concentration sample solution a, and the lower curve represents the high concentration sample solution a'. Note that as the aggregation progresses, the amount of transmitted light increases. A measurement procedure using the platelet aggregation ability measuring device having the above configuration will be explained. (1) Pour the same amount of sample solutions a and a' into both transparent storage containers 16 and 16', and place them in storage space 1.
7 and 17', and set the lids 1b and 1b'. Meanwhile, the aggregation promoter ADP is injected into the pipette 6. (2) The electric heater 2,
2' controls the temperature of the constant temperature units 1, 1' so that they are the same, and the motor control circuit 9 controls the speed of the stirrer motors 3, 3' so that their rotational speeds are the same. When the measurement starts, the photodiode 5,
The output voltages 5' are each amplified by an amplifier circuit 7, digitized by an A/D converter in an interface 10, and transmitted to a microprocessor 11. (3) Sample solution a,
While stirring a', the aggregation promoter ADP is injected from the pipette 6 into each of the transparent storage containers 16 and 16', but the amounts added to the two transparent storage containers 16 and 16' are made different. For example, the first
For the transparent storage container 16 of
ADP to the second transparent storage container 16'
Add 2 μM aggregation promoter ADP. Immediately after the injection of the aggregation promoter ADP into both sample solutions a and a' is completed, the manual start switch 6a is turned on. This starts the clock within microprocessor 11. (4) On the liquid crystal display 14, the horizontal axis shows the passage of time, and the vertical axis shows the photodiode 5,
The aggregation pattern, which is a temporal change in the optical output voltage inputted from 5' through the amplifier circuit 7, or the aggregation rate, is displayed. At the same time, the printer 15 prints the type of light output (aggregation pattern) and platelet aggregation ability pattern that change over time. Figure 4 shows the sequence of data actually obtained. Figure A is a combination of aggregation patterns {[A], [A]}, and the high concentration max is 10% or more, so the platelet aggregation ability pattern is [2]. Since Figure B is a combination of aggregation patterns {[A], [B]}, the platelet aggregation ability pattern is [3]. Figure C is a combination of aggregation patterns {[A], [C]}, and the low concentration max is less than 15%, so the platelet aggregation ability pattern is [4]. Since Figure D is a combination of aggregation patterns {[C], [C]}, the platelet aggregation ability pattern is [7]. The concentration of the aggregation promoter ADP added to platelet-rich plasma PRP is about 0.5 to 1 μM on the low concentration side, and about 2 to 5 μM on the high concentration side. Effects of the Invention According to this invention, there are the following effects. (a) inputting the detection signals of the first and second platelet aggregation detectors into the microprocessor;
Based on the maximum aggregation rate or the aggregation rate after a predetermined time for both detection signals, the aggregation pattern, which is a temporal change in the aggregation rate, is divided into two or more types, and platelet-rich plasma is determined from the combination of the types of aggregation patterns of both detection signals. Since the type of aggregation ability pattern is determined, there is no room for subjectivity, and the platelet aggregation ability pattern can be determined at extremely high speed and with high precision.
That is, there are no errors caused by individual differences that occur in visual observation, and it is possible to automatically measure the aggregation ability pattern with high reliability. (b) Since the display device displays the type of platelet aggregation ability pattern as the determination result of the microprocessor, the determination result of the microprocessor can be known quickly and clearly.

[Brief explanation of drawings]

FIG. 1 is a schematic configuration diagram including a block diagram of the entire platelet aggregation ability measuring device according to an embodiment of the present invention, and FIG.
The figure is a schematic flowchart of the operation, and A to G in Figure 3 are graphs of temporal changes in various aggregation rates.
FIGS. 4A to 4D are diagrams showing temporal changes in the aggregation rate actually displayed as data. A...First platelet aggregation detector, A'...Second platelet aggregation detector, a...First sample solution, a'...Second sample solution, 4...Light emitting diode ( 4'... Light emitting diode (second light emitter), 5... Photo diode (first light receiver), 5'... Photo diode (second light receiver), 11... Microprocessor, 14...Liquid crystal display (display device), 15...Printer (display device).

Claims (1)

[Scope of Claims] 1. A first transparent storage container for a first sample solution containing platelet-rich plasma and to which a high concentration aggregation-inducing substance is added, and a first sample solution in the first transparent storage container. a first platelet aggregation ability detector comprising a first light emitter/receiver that irradiates a light beam and receives the transmitted light beam to detect a temporal change in aggregation rate; and platelet-rich plasma in the first sample solution. A second transparent storage container for a second sample solution containing the same amount of platelet-rich plasma and to which a low concentration of aggregation-inducing substance is added, and a second sample solution in this second transparent storage container are irradiated with light. A second light emitter/receiver that receives the transmitted light beam and detects temporal changes in the aggregation rate.
inputting the detection signals of the platelet aggregation ability detector and the first and second platelet aggregation ability detectors, and calculating the temporal change in the aggregation rate based on the maximum aggregation rate or the aggregation rate after a predetermined time for both detection signals. a microprocessor that distinguishes two or more types of aggregation patterns, respectively, and determines the type of platelet aggregation ability pattern from a combination of the types of aggregation patterns of both detection signals; A display device for displaying a type of platelet aggregation pattern, which is a determination result obtained by the method. 2. The platelet aggregation ability measuring device according to claim 1, wherein the display device displays graphs of the temporal aggregation rates detected by the first and second platelet aggregation ability detectors, respectively. .