JPS6093357A - Automatic analyzing device - Google Patents

Abstract

PURPOSE:To eliminate variance in measuring data between samples when the sampling time is different by taking the sample of always the same item number into one sampling probe. CONSTITUTION:A sample 8 for the analyzing item requested is taken into a probe 1 by one sampling and is dispensed dividedly to reaction vessels 11 and 12 for respective items. Analysis of plural items is accomplished in this stage by sucking, discharging and distributing the sample 8 with one probe 1. The sample 8 is sucked, discharged and distributed into and from the inside of the probe 1 for the item not requested to be analyzed among the plural items so that the sample is stabilized irrespectively of the number of the items for analysis.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Application of the Invention] The present invention relates to an automatic analyzer, and more particularly to an automatic analyzer that aspirates samples for Ha items from the same sample into a sample probe in one sampling.

[R scene of invention]

In general, when analyzing multiple items during sample analysis, the state of the sample may change, and it is inefficient to collect the sample in several batches, so it is difficult to collect samples for multiple items at once into the sample probe by one sampling. It is being inhaled. In order to collect samples for a plurality of items in one batch, the conventional method is to inhale the required amount of sample depending on the number of requested items to be measured. but,
According to this conventional method, even if the measurement item is the same, the number of measurement items changes depending on whether the measurement target item is included or not, and the amount of sample inhaled changes each time. A cross-sectional view of the tip of the sample probe inside the temple glove is shown when the sample is inhaled. Figure (B) shows the state in which a sample is inhaled for a certain measurement item B. This figure 1 (A) shows that after inhaling air, which is a damping agent to prevent sample amount -19, and forming an air layer 14, a sample 16 for item B, which is necessary for the determination of item B, is inhaled, and item A is measured. The figure shows the state in which sample 17 for item A, which is necessary for the test, has been inhaled. X in Figure 1 (A) is the height of the sample for item A from the tip of the sample glove, and y in Figure 1 (5) is the height of the sample glove when inhaling the total sample for measuring both items A and B. Shows the height from the tip.

? Figure 81 ()J) is a diagram when a sample different from that in Figure 1 (A) is inhaled, for example, only item B, which is a sample that requires measurement. Zi-1,B in this Figure 1(B)
It shows the height from the tip of the sample glove when the sample measured for the item is inhaled. In this way, in Figure 1 (A) and Figure 1 (B), it is clear that when the sample for measurement of item B, which is inhaled to measure the same item B, is inhaled into the sample probe, Figure a11 ( For the sample shown in A) and the sample shown in Figure 1 (B), the moving distance from the tip of the sample glove until the sample for item B is discharged is the first.
There is a difference between y shown in FIG. 1(A) and 2 shown in FIG. 1(B). On the other hand, the sample probe is used for one cycle by cleaning the inside of the sample glove with a cleaning liquid (usually distilled water) for each sampling. For this reason, if the amount of sample differs each time the sample is sampled, the degree of dilution of the sample due to the small amount of residual liquid from the cleaning tPi that adheres to the inner wall of the sample glove will vary each time the sample is sampled. This difference causes variations in measurement data.

In this way, the measurement data is affected, for example in Figure 1 (A
) and the sample in Figure 1 (B), the measurement data of the sample in Figure 1 (Aj) is similar to the measurement data of the trial in Figure 1 (B) even for the same B item. Especially if the amount of sample for measuring item A is large and the amount of sample for measuring item B is small, the difference between y and 2 will also become large.
This effect on the measured data will also be greater. As described above, conventional automatic analyzers have the drawback that even if the same sample is used, measurement data varies due to different sampling times.

[Purpose of the invention]

An object of the present invention is to provide an automatic analyzer 4 in which no deviation occurs in the it+lJ constant data between samples even if sampling times are different.

[Summary of the invention]

In the present invention, when inhaling samples for accidental items from the same sample into a sample probe during one sampling, the same number of items can be collected in one sample probe public regardless of whether or not there are requested items for division. The idea is to reduce the variation in measurement data between samples by always keeping the same dilution of the sample due to the residual cleaning solution adhering to the inner wall of the sample probe after inhaling the sample.

[Embodiments of the invention]

Hereinafter, implementation of the present invention will be explained.

FIG. 2 shows a schematic diagram of the vicinity of the sample probe to which the present invention is applied, and FIG. 3 shows a sectional view of the tip of a sample probe showing an embodiment of the present invention. There is.

In FIG. 1, a sample glove 1 for aspirating a sample for measurement is provided on a sample syringe 2. This sample syringe 2 is equipped with a pipe line 9 for supplying distilled water 13, and a solenoid valve lO for controlling supply and stop of distilled water 13 is provided in this line. Also, inside this sample syringe 2 is a sample glove l.
A plunger 3 used for aspirating a sample is fitted so that it can move up and down. This plunger j is fixed to the slider part 4, and this plunger part 4 is installed in the pulse motor 6 and is attached to the slider part 4.
It is configured to move up and down by two hooks 5 that mesh with each other. In FIG. 1, 8 and 9 are samples, 11. '12 is a container,
19 is a cleaning tank, and 20° 2J is a new container.

Next, the operation of fcmlr will be explained. When the pulse motor 6 rotates, the pinio/7 rotates, and the rotation of the pinio/7 moves the rack 5 and moves the slider part 4 up and down. This vertical movement of the slider part 4 causes the Pushinger 3, which is directly connected to the slider part 4, to move up and down. - Open the electromagnetic valve 10 and fill the interior of the Scenegle globe 1 with distilled water 13. First, air is sucked into the sample glove l by lowering the plunger 3 in the sample link 2. By inhaling this air, air 7
1kv14 is formed. This air layer 14 is formed to prevent the concentration of the sample from being diluted by the distilled water 13 when the sample comes into contact with the distilled water 13 filling the sample globe 1. . Next, after lowering the sample glove 1 into the sample 8, IIT for the items that need to be analyzed for the sample 8 is sucked into the sample glove 1 by further lowering the plunger 3. The sample sucked into the sample glove 1 is dispensed into containers 11 and 12 in an amount necessary for each analysis item. After the partial pressure, the sample glove 1 is moved to the cleaning tank 19,
Thereafter, the electromagnetic valve 10 is opened and distilled water 13 is supplied to discharge the ψ′A layer 14 taken into the sample glove 1 and wash the inner wall of the sample glove 1.At this time, the plunger 3 In addition, by repeating the same procedure for sample 8 as for sample 9, dispensing for multiple samples can be performed. Containers 11 and 12 are also transferred sequentially. and a new sample container 20.21 is set.

Next, the state of the tip of the sample glove 1 according to the present implementation ft1J will be explained using FIG. 3.

FIG. 3(A) shows the state of sample suction at the tip of the sample probe l when two measurements, A and B, are requested for a certain sample. That is, first, the air layer 14
After inhaling, sample 16 for measurement of item B and sample 17 for measurement of item A are inhaled in sequence. The height from the tip of the sample probe 1 to the sample position for measuring item B at this time is defined as y.

The sample sucked into the sample probe 1 is discharged in the reverse order of suction. Also, Figure 3 (B
) shows the sample inhalation state of the sample glove 1 when there is a request for only 4 BJJ tests for a certain item. This figure 3 (B) shows that after first inhaling the air layer 14, the sample 16 for measurement of item B is inhaled, and then, although there is no request for measurement of item A, the sample 16 for measurement of item A is not requested. Inhale the sample four times as necessary to measure item A. This is sample 18 for item AD. In this way, sample 18 for item AD
By inhaling, the force at the tip of the sample glove l
The height to the sample position for the "B item 1tll" run is the same as in the case shown in FIG. 3(A). Therefore, the degree of dilution due to residual distilled water adhering to the inner wall of the sample glove will be the same whether two items are requested rarely or only one item is requested, and the variation in 6111 constant data between samples will be the same. It disappears. Further, the sample is discharged in the reverse order of suction as in the case of FIG. 3(A). In this case, the item AD sample 18 is also discharged into the container like the item AJ+1 sample 17, but no measurement data is outputted. This measurement uses '1'P (total/Pakun) and kLB (Al゛min) as measurement boundaries.

This 611j constant is to see how much the 6411j constant of TP changes depending on the presence or absence of a measurement request for AlI3. In addition, in this measurement, in order to clarify the influence of the presence or absence of an item request on the direct measurement, the sample for TP measurement was 3 μt, and the sample for kLB measurement was 10 μt.

However, the concentration unit of TP is g/dL. From Table 1, it is clear that in the case of this example, the data variation is improved by 5% or more compared to the conventional example.

Therefore, according to this implementation sequence, when samples for multiple analysis items are aspirated into the sample probe in one sampling, even if the number of analysis items for each sample varies, the sample Variations in <3111 constant data based on differences in the amount of sample aspirated into the probe can be eliminated.

In addition, even though there is no measurement request, the sample ADtljt for item A measurement cycle will be inhaled and expelled even though it is not used, but recently the required sample for 1 item is p3μt per item.
The amount is as small as ~10 μl, and the use of extra sample is only required when the number of measurement requests for the sample probe is reduced, and does not cause any practical problems.

In addition, in this example, 2 analysis items for each sample are raised by 9, but it is not limited to 2 items, but -C is also applied to multiple items, regardless of whether there is a request for measurement. By inhaling the sample first, it is possible to eliminate variations in data due to dilution of the sample.

Furthermore, although the illustrated embodiment in FIG. 2 shows the case where one sample probe is used, the sample glove is not limited to one true ridge, and can be used on a lifting platform using multiple fla glue and one single glove. You can also rte in the same way. If several of these sample gloves "k61" are used, the number of analysis items to be inhaled into one sample glove will be reduced, so if there is no request for a measurement item, there will be less extra sample to be inhaled, which is more effective.

In addition, in the one:fin multi-item analysis, a discharge container for unrequested items can be provided in order to improve economy and processing efficiency without creating a reaction container for unrequested items.

〔Effect of the invention〕

As explained above, according to the present invention, it is possible to prevent variations in measurement data between samples even if -y knobling times are different.

[Brief explanation of drawings]

Figure 1 (Al (to) is a diagram showing the sample suction state of a conventional sample glove, Figure 2 is a partial schematic diagram of an automatic analyzer to which the present invention is applied, Figure 3 (A) 1.131 is It is a diagram showing the sample suction state of the sample glove according to the present invention. 1...Sample glove, 2...Sandal sleeve/jet, 3...Plunger, 4...Slider Part, 5
...Cook, 6...Pulse motor, 7...Hinion, 819...Sample, 10...Solenoid valve, 11.12
... Container, 13... Distilled water, 14... Constricted air layer, 1
9...Cleaning tank. Agent Patent Attorney Tatsuyuki Unuma Figure 1 (A) (F3) 11 arrows, 11 enemies Figure 2 Figure 3 (A) 11 disasters, length 11 caps

Claims (1)

[Claims] 1. In an automatic analyzer that aspirates the sample for the requested analysis item into the sample probe in one sampling and divides and dispenses it into reaction containers for each item, one sample probe is used to aspirate the sample to be analyzed. The sample is aspirated and dispensed into the sample probe to analyze multiple items, and even for items that are not requested to be analyzed among the multiple items, the sample is aspirated and dispensed into the sample probe, and the sample is analyzed regardless of the number of analysis items. An automatic analyzer characterized by being stable.