JPS6093339A - Electrophoretic device - Google Patents

Abstract

PURPOSE:To enlarge and display an electrophoretic image to perform manual graduation accurately by converting the coordinate position of a displayed electrophoretic image pattern in accordance with designated magnifications. CONSTITUTION:A prescribed magnification is designated by an enlargement switch 22a on a keyboard 22, and a coordinate reference position to be enlarged on a CRT18 is designated by a light pen 23. Then, an operating device 17 obtains a constant by which the designated coordinate position is a reference position, and coordinates are converting successively by multiplication of the designated magnification. The electrophoretic image read out from an RAM16 through a microcomputer 21 is displayed in converted coordinate positions on the CRT18, and it is enlarged and displayed. Consequently, detailed data can be displayed, and the resolution of a low-density part to be graduated is enhanced, and manual graduation is performed accurately.

Description

DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention relates to an electrophoresis device used for analyzing blood H1 protein, for example, in which fractionation points are manually determined from an electrophoretic image pattern displayed on a display device using a manual. There is C in b related to an electrophoresis device as described above.

(Prior art) In addition to displaying the actual analog pattern shown in Figure 11 using a conventional electrophoresis apparatus such as a seeder with high resolution, CR
As shown in Fig. 2, display the entire electrophoretic image pattern of IDj on T, and from the electrophoretic image pattern displayed on this CRl, divide points by manual means such as a board, etc. Manually correct the fraction %, etc.
There is something that I tried to do.

Here, the normalization of the analog pattern [,41,
The photometric data used to create the image is usually 8 to 16 bits 1 to △7.
/1) C is performed by A/D conversion with a converter,
In addition, CRTs are raster scan type, and the standard CRT has approximately 560 dots horizontally and 216 dots vertically, and the high-resolution CRT has approximately 640 dots horizontally and approximately 512 dots vertically. .

When graphically displaying this J: sea urchin migration image pattern on a CRT, as shown in Figure 3, the CRT
1's 1 dot 1~ corresponds to 1 pit 1~ J-ru bidet JRAM
2, the A/[] converted photometric data is stored in the video RAM 2, and the stored data is read out and displayed on the CRT-1. As shown in FIG. The A/D converted photometric data is supplied from the monitor 3 of the digital converter 3 to the video RAM 2, and the photometric data is stored in the corresponding address of the video RAM 2. The data thus stored in the video ARΔM2 is synchronized with the readout signal by converting the clock signal from the clock generator 5 into a continuous CRT scan address in the address selection mechanism 4 and supplying it to the video RAM 2. The data is sequentially read out from shift I to register 6, and shifted one bit at a time by the dot clock signal i44 from shift I to register 6, and then transferred to CRT 1.
The data is supplied to the computer for display purposes.

However, such a conventional electrophoresis station [Beni J31+X
So, for example, the analog pattern is 12 hits j・σ)△/
'Using a D converter, normalize: +(ioX 216
When displayed on a CRT of 1ζ・)1-, in this case σ)0
In 1, -1-G, Lt, and L only 8-bit information is displayed. '

In addition, a normal CRT has a length J as shown in Fig. 2.
Since information other than the image pattern is also displayed at the same time, the electrophoretic image pattern is CRrσ) 1iiq 1r
(ii) If you try to display J in half, a lot of information from Pi1~ will be thrown away and it will be difficult to display it.(4C)
20) When performing manual fractionation,
) + fG + t< When the entire turn is displayed on a CRT, CRTσ) It' is fractionated due to resolution.
(The LCi 1M degree has a poor 9M filtration, which makes the desired fractionation position unclear, and it has the disadvantage that manual fractionation cannot be performed accurately with iJT.

OBJECTS OF THE INVENTION It is an object of the present invention to eliminate the above-mentioned drawbacks and to provide an electrophoresis apparatus suitably configured to perform manual fractionation accurately.

(Summary of the Invention) The present invention is based on an electrophoresis apparatus in which fractionation points can be input from an electrophoresis image pattern displayed on a display device in one step. ) a means for specifying an arbitrary coordinate position of the electrophoresis IK+ pattern displayed on the display device, and 18 of the electrophoresis image pattern to be displayed on the display device;
One step to manually adjust the rate and the specified coordinate position i%
J. Coordinates specified based on the manually applied magnification (1/coordinates 1 of the displayed electrophoretic image pattern with the stomach as the reference)
The coordinates are transformed in accordance with the input A8 rate, and the coordinate-converted electrophoretic image pattern is displayed on the display at 1°, and the displayed desired 1g rate is displayed. Manipulating the fractionation points from the electrophoresis image pattern? This device is characterized in that it is configured so that a file C can be input.

(Example) Figure 5 shows electrophoresis of the present invention! 1 is a block diagram showing a circuit configuration of an example of an l+IJ device. FIG. The electrophoretic image formed on the support 11 is conveyed between the lamp 13 and the light-receiving element 14 by the input control unit 12 and combined, and the output of the light-receiving element 14 is output as necessary (logarithmically amplified and Δ /D converter 15
Enter. The analog pattern data converted into a digital signal by this Δ/1 converter 15 is transferred to the video 7
After 1RΔM is stored in the clock 16, it is processed by the arithmetic unit 17 as required, and then displayed on the CRT.
18. The data is output to the recorder 11) and the printer 20 for display. J5, the control of these parts is micro.
J, -) "C?1" is sent to the printer 21.

In this example, various switches such as an enlargement switch 22a, a reduction switch 22b, a segmentation point addition switch 22C, and a segmentation point deletion switch 22d are provided in the hair neatlet 22 into which external information is input. The microcomputer 21 detects the coordinate position on the CRT 18 by touching the light pen 23.
so that it can be taken into account.

The operation of this embodiment will be explained below. When displaying the entire electrophoretic image pattern on the CRT 18, the arithmetic unit 17
5, for example, the magnification N in sequential 12-bit data
The value obtained by multiplying by
Transform the coordinates of the stem, and sequentially set '1' to the position that indicates the height of the value.
``S'Z'' (by doing so, the entire display is displayed.

In addition, when enlarging and displaying the electrophoretic image pattern on the CRT 18, scan the enlargement switch 22a on the keyboard 22 to specify the predetermined ratio N, (reduction 2N1 × N2). CR-11 by Rai 1~Ben 23
8J's expanding standard L (specify the target position, lie in the performance test 17) - Find a constant such that the target position 1σ specified by the pen 23 becomes i\, and then Coordinate transformation is performed using the constant and the value obtained by multiplying the data of sequential logic by the specified magnification N2. By raising it up, an enlarged image is displayed.At this time, by enlarging it, the display area is exceeded and the IC data is fixed at the upper limit value or not displayed.

Furthermore, when displaying the electrophoretic image pattern in a reduced size on the CRT 18, operate the reduction switch 22b of the keyboard 22 and the light pen 23, perform the same operation as the enlargement mode described above, and reduce the size 19 on the CRT+8. Display J.

Figure 6 shows the display mode of the electrophoretic image pattern with CI'< T 111.1 in enlarged mode. This is an enlarged image based on the l-coordinate position. In this embodiment, the electrophoretic image pattern enlarged and displayed on the CRT 18 is used to select the fractional point addition switch 22c (or fractional point deletion switch 22d) on the keyboard 22.
While operating , specify the fractionation point using Rai 1 to Ben 23, and this will set the rIJLq fraction % customer to 31 sections and display the CRT.
Display on 18.

FIG. 7 is a flowchart showing the operation of this embodiment described above.

Note that the present invention is not limited to the above-mentioned example, and can be modified or changed in many ways.

For example, estimating the coordinate position on the CRT 1g can be done not only with the light pen 23, but also by displaying a cursor on the CRT 18. In addition, in the enlargement mode, it is possible to enlarge and display not only the vertical direction but also the horizontal direction, and it is also possible to enlarge and display only the desired part of the entire display. Furthermore, for various calculations such as coordinate transformation, it is possible to use the operation function of the micronviewer 21 without using the arithmetic unit 17.The display device is not limited to CF<1; - It is possible to use a Ftrix liquid crystal display.

(Effects of the Invention) As described above, according to the present invention, the electrophoretic image pattern can be displayed in an enlarged size, and detailed data that was conventionally discarded can be displayed. The resolution of fractionation/jiiL (11×1 degree 9B) can be increased, and therefore manual fractionation can be performed accurately.

[Brief explanation of drawings]

Figure 1 is a diagram showing an analog pattern of a migration image, Figure 2 is a diagram showing the entire migration image pattern displayed on a CRT, and Figure 3 is a diagram to explain the relationship between a CRT and 4RAM. Figure 5 is a block diagram showing the circuit configuration of an example of the electrophoresis device of the present invention; Fig. 5 shows a table of the electrophoresis fII image pattern on a CRT in J3 magnification mode in the electrophoresis apparatus 1, the mode of display is not shown. Fig. 7 is a flowchart showing the operation of the electrophoresis apparatus shown in Fig. 5. Char 1~. 11...Support 12...Enter/J system 911 part 13.
...Lamp 14...Light receiving element r15...A/D converter 16...Memory 17...Arithmetic unit 18...
CF< 119...L/ml-da 2o...Printer 21...Microphone 1 Combi coater 22...Keyboard 22a...Enlargement switch 22
1)... Reduction switch 22c... Fractional point addition switch 22d... Fractional point deletion switch 23... Lie 1-pen. Q'r i'l Applicant Olympus Optical Industry Co., Ltd. Figure 3 Figure 4 Figure 5 Figure f'/Figure 6 Figure 7 Procedural Amendment November 1981 1. Indication of Case 1988 Special Al Application No. 201278 2, Name of invention Electrophoresis device 3, Person making amendment Related to the case Patent applicant (03'7) Olympus Optical Industry Co., Ltd. Telephone (
58]) Column 7 of 2241 Parties (Representative) Explanation, contents of amendment (as attached) 1. Lines 8 to 18 of page 1 of the specification are corrected as follows. ``Claim 2 of Claim L: In an electrophoresis apparatus in which fractionation points can be manually input from the electrophoretic image pattern displayed on the display device, means for inputting the magnification of the electrophoretic image pattern to be displayed on the display device. and means for converting the coordinate position of the displayed electrophoretic image pattern based on the magnification Δ. An electrophoresis apparatus characterized in that it is configured such that fraction points can be manually input or deleted from an electrophoretic image pattern at a magnification of In the 14th line of the same page, "by entering" should be corrected to "by entering or deleting." 3. Change "for, about" to "for, about" on page 4, line 10 of the same page.
Correct it to "approximately the lower order of 2 bits", and amend lines 19-20 of the same page to "ma because the position to be segmented becomes unclear due to resolution." 4. Correct page 6, lines 10 to 20 as follows. In an electrophoresis apparatus, the apparatus further comprises means for inputting a magnification of the electrophoretic image pattern to be displayed on the display, and means for converting the coordinate position of the displayed electrophoretic image pattern based on the input magnification. The system is characterized in that the coordinate-converted electrophoretic image pattern is displayed on the display device, and the fractionation point can be manually input or deleted from the turn of the displayed electrophoretic image at a desired magnification. 5. Correct the 7th line of the 7th page to ``Convert the coordinates to the address of and the height of the converted value'', and change ``scan'' to ``operate'' in the 12th line of the same page. Correct. 6 Change "Can do. Also" in the Φ line of the 9th page of the same page to "Can do it." A key to move to the next dividing point in the direction, a key to move to the next dividing point in the left direction, etc. will be provided.Also," was corrected, and "Full display... enlarged" in lines 6-7 of the same page. ” was corrected to ``Fixedly enlarge only the desired part of the entire display without specifying the reference coordinate position,'' and the 12th line of the same page was changed to ``Using a display device.'' It is also possible to make fractional corrections by enlarging and printing on recording paper.''

Claims (1)

[Claims] 1. Install the electrophoresis device into which the fractionation points can be manually input from the electrophoresis image pattern displayed on the display device.
means for specifying an arbitrary coordinate position of the electrophoretic image pattern displayed on the display device, means for panning the electrophoretic image pattern (7'< Based on the coordinate position IM'J3 and the input magnification, the coordinate position of the displayed electrophoretic image pattern is converted into the 1'C coordinate according to the input ratio. This P31 standard-converted electrophoretic image pattern is displayed on the display device 1, and fractionation points can be manually determined from the displayed electrophoretic image pattern at a desired magnification. An electrophoresis device characterized by the following structure.