JPS60895A - Process and apparatus for metabolizing organic waste water using growth liquid slurry containing clay, sludge, sulfur bacteria, purple sulfur bacteria, and mixed strain of euglena - Google Patents

Abstract

PURPOSE:To remove BOD of organic waste water effectively and to metabolize it to feed or fertilizer by treating org. waste water using growth liquid slurry contg. clay, sludge, sulfur bacteria, purple sulfur bacteria, and mixed strain of Euglena as a filter medium and culture base material. CONSTITUTION:1t of swine urine contg. 5,500ppm BOD component is charged to a storage tank 1, and 1t growth liquid comprising sulfur bacteria, purple sulfur bacteria, mixed strain of Euglena is inoculated and cultivation is carried out at 15-32 deg.C for 5 days. A slurry mixture consisting of sludge and waste clay in 2:8 proportion is filled in a transparent vertical cultivation tank 2, 3, 4, 5. Filtration membranes 11, 12, 13, 14 are attached to the tanks; 0.5t filthy water comprising swine urine contg. 2,500ppm BOD component is fed every day from a filthy water feeding port 39, and treated water contg. 15ppm BOD component is obtd. Growth liquid contg. sulfur bacteria, purple sulfur bacteria, and mixed strain of Euglena is fed from the transparent vertical cultivation tank 2 to a fermentation tank 37 using a liquid feeding pump 23 and a valve 36. slime bacteria and yeast are inoculated after adding saccharide and fermentation is performed.

Description

[Detailed Description of the Invention] The present invention utilizes organic wastewater as a filter medium and a culture substrate, using a mixed slurry of clay, sludge, sulfur bacteria, purple sulfur bacteria, and euglenoid mixed strain growth liquid, and by passing it through this mixed slurry. By promoting flocculation and separation of sludge, measuring the adsorption of purple sulfur bacteria and euglenoid species to clay and sludge, and providing a suitable "home" for these bacteria, organic wastewater can be efficiently treated. This article relates to a method for obtaining and utilizing mixed algal cells of sulfur bacteria, purple sulfur bacteria, and euglenoid species, and the coating thereof.

Conventionally, there is an activated sludge method as a method for treating organic wastewater such as sludge using microorganisms. This treatment method utilizes the utilization of organic matter by unspecified aerobic microorganisms in sludge to carry out purification treatment through a high aeration operation.

A similar aerobic treatment includes a biofilm method using a one-rotation disk in a shelf water filter. In this aerobic treatment method, the quality of the treated water is unstable, the treatment facility requires a large area, and the operating costs for treatment are enormous. Also, because unspecified microorganisms are used, it cannot be recycled into resources. The present invention improves the problems of conventional aerobic treatment methods such as the activated sludge method and the main substance film method, and promotes stable growth of specific microorganisms, biological fixation of organic matter, sedimentation adsorption to clay, and floc formation. 71 We aim to facilitate this passage, stabilize the quality of treated water, reduce the number of treatment days, treatment area, and costs, and also return and use the growth liquid of sulfur bacteria, purple sulfur bacteria, and Euglena species mixed strains, and Feed, fertilizer (soil conditioner), etc. can be produced as a result of sludge extraction and conditioning. Particularly in the case of fertilizer, saltpeter can be obtained by precipitation due to the combination of oxygen produced by algae and ammonia and potassium from the decomposition of organic matter. When acid clay and waste clay are used, an organic heat-like mixed I-effective nitrogen fertilizer can be produced. In addition, 1000ml of ubiquinone, a physiologically active substance contained in the purple sulfur bacteria, can be used as feed. It is possible to increase the growth rate of livestock such as pigs and poultry by 20% by feeding them with M-water containing 10 to 12 mg of raw (meat), and it is also possible to produce feed additives. In order to meet these demands, the present invention inoculates and cultivates a mixed strain of sulfur bacteria, purple sulfur bacteria, and Euglena species in organic wastewater, and uses the growth liquid cyclically to purify organic wastewater. This is an attempt to provide a method for spraying fertilizer and feed using an integrated system that reduces force and is economical. In general, the surface of a solid such as clay is charged in a solution, and as a result, the surface of a solid such as clay is electrically charged. Ions with this charge are attracted near the surface and form an ion layer with a high ion concentration near the surface.It is known that cations form a cation layer, while anions form an anion layer. Bacteria in the water have cations and are adsorbed to the clay surface along with other nutrient ions.

The concentration of hydrogen ions becomes high near the interface. Therefore, the activity of microorganisms is suppressed in the ion layer near the interface, and sedimentation adsorption and floc formation progress, and separation of the solution and the ion layer progresses, making the upper surface transparent and BOD removed and purified. The sulfur bacteria specified in the present invention, the purple sulfur bacteria, and the mixed strain of Euglena species, are able to settle, adsorb, and infiltrate into the mixed slurry of clay and sludge in which ions, cations, and anions are present, multiply well, and assimilate organic matter. It has become clear that clay, sludge, and mixed slurry provide good "homes" for these specific microorganisms, and that they often suppress the growth of unspecified heterotrophic bacteria in their culture substrates and create p-codominant species (Oo-dom).
inant 5pecies) and forms low temperatures (winter)
It was found that it is a functional microorganism that can easily assimilate organic matter in response to high temperature (summer) conditions. Therefore, based on the principles described above, the fallen ion layer and the solution are separated, and the upper surface of the final solution becomes transparent and purified.

The clay, sludge and microorganisms used in the present invention are as follows.

(1) Clay The best clay is what is generally called clay, acid clay, or waste clay. Table 1 shows the classification of soil particles according to the International Soil Science Society method.

Table 1 (2) Sludge The sludge used is sedimented sludge from sewage ditch 1 yh urine tank, etc. at the following mixing ratio.

Mixing ratio: Clay 8: Soil F2.

(3) Microorganisms In the present invention, sulfur, yellow sulfur bacteria, and purple sulfur 1 bacteria are simply referred to as purple sulfur bacteria.

(A) Purple sulfur bacterium chromatium vlnosumOhromat
ium minius Thiospirillum jenenseThio
cystis gslatinosaBegg:Lat
oa alba Beggiatoa thiotrix (B) Euglena gracilis Euglena oxyuris Kuglena oblongs The above Eudalena species are widely distributed in the natural world, and are particularly often found in reservoirs with high organic matter concentrations, co-dominant with purple sulfur bacteria. form the dominant 5 pecies , can grow both autotrophically and heterotrophically, and under light conditions, BOD removal is achieved through photoautotrophic and photoautotrophic growth. Oxygen generated by photosynthesis of euglenids is directly utilized by vine microorganisms through heterotrophic metabolism.

(0) Amimidomouth Hydrod-1otyon retioulatum
(D) Myxobacteria (a) Ohondrom7oes Genus Ohondro
myoes arooatus Ohondromyoe
s apiulatus Ohona rornyces
aurantolaousohonaromyces
bruneus (0) Mixooo, aus Genus Mixocoua fulVus Mixocous rubesoensMixoco
cus albus Mixocous brepipes) Oytop
Oytophaga hutehinaeniiOyt
ophaga anitrataOytophaga
albohgilhaOytophaga anu:L
aris Sporocytophaga genus Sp
orocytophaga myxooocoides
Sporoytophaga oauliformi
sSporooytophaga congreget
eSporocytophaga ellipsosp
ora(E) Bacillus JIS Bacillus megathoriumAerob
acter levamicumLeuconosto
off1esentroidos The above-mentioned mucilage is a bacterium that forms mucilage by decomposing sucrose and other carbohydrates and proteins, and one or more of the above species can be inoculated in a mixture.

(E) Yeast sacharomyoeg ellipes-olde
usOancLida utilis Torula utilis Wild yeast is used, but one of the above species or a mixture of loquat is mainly inoculated.

In the present invention, by allowing three types of microorganisms such as sulfur bacteria, purple sulfur bacteria, and euglenoid bacteria to dominate, a high concentration of bacterial cells can be maintained and BOD removal can be performed efficiently, and it can also withstand changes in seasonal environmental conditions. The symbiosis of the three species can maintain a stable microbial flora and biological ability.

Embodiments of the present invention and their principles will be described below with reference to the drawings.

Sewage storage tank l Transparent three-dimensional culture tank 2, 3, 4, 5 Fermentation tank 37
The various types are connected via the flow vibro, 7, 8, and 9 liquid sending pumps 43, and the discharge pipe 10 is connected.

Transparent three-dimensional culture ear p2, 3, 4.5 with filtration membrane 11, 12
．． 13. Valves 19, 20, 21, 22 are provided at the bottom of each i4 tank 26, 27, 28, 29 and connected to the sludge drying and dewatering tb equipment 2b via a mono pump 24 for sludge i# section. Transparent standing 1/i! Culture Hz l 31
4 The top surface of t5 is closed and gas vents 26, 27, 2
8,29. Waterproofing first come first served 30.31. ．． 3j2,33
, an ultraviolet germicidal light 38 is provided. Transparent three-dimensional culture tank 2, 3,
Various types of stirring machines 5, 16.
17 Attach and return Vibrator 3 from transparent 3D mumg
4. Return pump 23. The sewage storage tank 11 is connected to the fermentation tank 37 via valves 35, 36. Fermenter 37. Each sewage storage tank l has a sewage inlet 39. Blower 41. Air jet pipe 40. Liquid feed pump 42. Equipped with. An Amidoro tank 18 is installed on the bulk powder. The principle of the device is as above. Pig urine It with a BOD j of 500 ppm was introduced into a sewage storage tank L, and 1 t of a mixed strain growth solution of sulfur bacteria, purple sulfur bacteria, and Euglena was inoculated and cultured at 15 to 32°C for 5 days.

Transparent three-dimensional culture tanks 2, 3, 4.5 are filled with slurry mixing tubes of sludge 2 = waste white clay 8, and filter membranes 11, 12 are filled.

13.14 (100 mesh) was added to the sewage inlet 39, and pig urine sewage with a BOD of 2500 ppm was collected.
st t - was introduced every day to obtain treated water containing 15 ppm of bulk powder. Sulfur bacteria from transparent three-dimensional culture tank 2 Purple sulfur bacteria
Return pump for euglenoid mixed stock growth 23. The pulp is sent to fermentation [37] through pulp 36, 2% carbohydrates are added, mucus bacteria and yeast are inoculated, and the mixture is fermented for 4 hours at 25-32°0 to produce 0.25 t of liquid fermented feed every time. Obtained. Valve 1
9.20, 21, 22, 45.・20 kg of sludge fertilizer is dried in the sludge drying device 25 via the mono-pump 24
/f4 was obtained in the transparent three-dimensional culture tank, and 2, 3, and 4.5 were each filled with 2 kg of waste white clay in July.

Below are the effects and advantages of the present invention! It's like +4 (
1) Facility costs can be reduced to one-fifth of the conventional costs.

(2,) B OD 15p without using flocculant
Treated water with a pm or less concentration can be obtained.

(3) Can be used as feed and fertilizer containing physiologically active properties (4)
) Odor pollution can be prevented.

[Brief explanation of drawings]

The drawings are cross-sectional RFE diagrams illustrating embodiments of the present invention and their implementation and aspects.

Claims (2)

[Claims] (1) Organic wastewater is assimilated by passing slurry of clay, sludge, sulfur bacteria, purple sulfur bacteria, and Euglena mixed strain growth liquid through a 9-diagnosis filtration material and culture substrate. Features include clay, sludge, sulfur bacteria, purple sulfur bacteria, and a method for assimilating organic wastewater using slurry of mixed growth liquid of Euglena species. (2) Configure storage i1 + transparent three-dimensional culture tanks 2, 3, 4.5, and sewage storage tank 1. Sludge regulating valves 19, 20, 21, 22, 45 are attached to the lower part of each transparent three-dimensional culture tank, and connected to a sludge dewatering device 25 via a sludge regulating mono-pump 24. Sewage storage tank l Transparent three-dimensional culture tank 2, 3, 4.5
are connected via the pump 26 advection vibro, 7, 8.9, and the discharge pipe 10 is attached. Transparent three-dimensional culture tank 2,
3, 4゜5 are filter membranes 11, 12, 13, 14, stirrer 15゜16 t 17 + light sources 30, 31, 32, respectively
, 33. Gas venting 26, 2, 2829 is carried out from the transparent three-dimensional culture tank in the warehouse where the growth liquid of red sulfur bacteria and red sulfur bacteria can be collected.Return pump 23Return pipe 34Pulp 35
, 36, respectively, to the sewage storage tank, 1 acid tank, 3 tanks, and the 9 seeds can be used as a fermentation substrate.
An assimilation treatment device using clay sludge of organic wastewater, sulfur bacteria, purple sulfur bacteria, and mixed slurry of growth liquid of mixed strains of euglena species, which promotes sedimentation and attachment of purple sulfur bacteria, and is characterized by attaching a 7-mimidoro tank 18 to driftwood.