JPS6049262A - アデニン自動分析装置 - Google Patents
アデニン自動分析装置Info
- Publication number
- JPS6049262A JPS6049262A JP15725483A JP15725483A JPS6049262A JP S6049262 A JPS6049262 A JP S6049262A JP 15725483 A JP15725483 A JP 15725483A JP 15725483 A JP15725483 A JP 15725483A JP S6049262 A JPS6049262 A JP S6049262A
- Authority
- JP
- Japan
- Prior art keywords
- column
- adenine
- reaction
- eluate
- bromoacetaldehyde
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 229930024421 Adenine Natural products 0.000 title claims abstract description 23
- 229960000643 adenine Drugs 0.000 title claims abstract description 23
- 238000004458 analytical method Methods 0.000 title claims abstract description 20
- NMPVEAUIHMEAQP-UHFFFAOYSA-N 2-Bromoacetaldehyde Chemical compound BrCC=O NMPVEAUIHMEAQP-UHFFFAOYSA-N 0.000 claims abstract description 49
- 238000006243 chemical reaction Methods 0.000 claims abstract description 33
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 5
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 4
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 4
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 4
- 239000003480 eluent Substances 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 5
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 5
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 238000001816 cooling Methods 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 14
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 12
- 238000000926 separation method Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 5
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 4
- 239000010839 body fluid Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 210000004556 brain Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000002372 labelling Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- -1 animals and plants Natural products 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000012916 structural analysis Methods 0.000 description 2
- QSKPIOLLBIHNAC-UHFFFAOYSA-N 2-chloro-acetaldehyde Chemical compound ClCC=O QSKPIOLLBIHNAC-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 241000254158 Lampyridae Species 0.000 description 1
- 241000123069 Ocyurus chrysurus Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 229960001456 adenosine triphosphate Drugs 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- XONPDZSGENTBNJ-UHFFFAOYSA-N molecular hydrogen;sodium Chemical compound [Na].[H][H] XONPDZSGENTBNJ-UHFFFAOYSA-N 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15725483A JPS6049262A (ja) | 1983-08-30 | 1983-08-30 | アデニン自動分析装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15725483A JPS6049262A (ja) | 1983-08-30 | 1983-08-30 | アデニン自動分析装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6049262A true JPS6049262A (ja) | 1985-03-18 |
| JPH0544623B2 JPH0544623B2 (enrdf_load_stackoverflow) | 1993-07-06 |
Family
ID=15645626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15725483A Granted JPS6049262A (ja) | 1983-08-30 | 1983-08-30 | アデニン自動分析装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6049262A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61254852A (ja) * | 1985-05-08 | 1986-11-12 | Shimadzu Corp | ヒスタミンの分析方法及び装置 |
| JPS6438636A (en) * | 1987-08-05 | 1989-02-08 | Ajinomoto Kk | Fluorescent analyzer |
| JPH03175356A (ja) * | 1989-12-04 | 1991-07-30 | Res Dev Corp Of Japan | 核酸構成成分過酸化物による核酸損傷程度測定方法および測定装置 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5529791A (en) * | 1978-06-14 | 1980-03-03 | Bifok Ab | Method of continuous measuring |
-
1983
- 1983-08-30 JP JP15725483A patent/JPS6049262A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5529791A (en) * | 1978-06-14 | 1980-03-03 | Bifok Ab | Method of continuous measuring |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61254852A (ja) * | 1985-05-08 | 1986-11-12 | Shimadzu Corp | ヒスタミンの分析方法及び装置 |
| JPS6438636A (en) * | 1987-08-05 | 1989-02-08 | Ajinomoto Kk | Fluorescent analyzer |
| JPH03175356A (ja) * | 1989-12-04 | 1991-07-30 | Res Dev Corp Of Japan | 核酸構成成分過酸化物による核酸損傷程度測定方法および測定装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0544623B2 (enrdf_load_stackoverflow) | 1993-07-06 |
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