JPS6046084B2 - cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to cosmetics whose quality is stabilized by antioxidants.

As is well known, some of the substances that make up cosmetics are gradually oxidized by oxygen in the air, and deterioration due to oxidation not only deteriorates the original efficacy of the cosmetics, but can also cause damage to the skin. It can also cause inflammation.

For example, the oils and fats that are always included in emulsions and creams are gradually oxidized on the skin during storage or after use, producing peroxides (lipid peroxides), which can cause inflammation on the skin. It has been revealed that it is an extremely undesirable substance, as it causes the formation and deposition of pigments, weakens the skin's resistance, makes it more susceptible to various skin disorders, and even accelerates skin aging. Therefore, preventing oxidation and maintaining stable quality is an extremely important issue when manufacturing cosmetics. Antioxidation can be prevented to some extent by selecting storage containers and storage conditions, but what is most effective is the use of appropriate antioxidants. In other words, by appropriately using antioxidants such as BHT) BHA) propyl gallate, the quality stability of cosmetics can be dramatically improved, accidents caused by deterioration can be reduced, and accidents caused by deterioration during the distribution stage or during use can be dramatically improved. If you have no choice but to dispose of it, there will be almost no waste. It is known that the above-mentioned skin damage caused by lipid peroxide is also caused by sebum peroxide secreted by the skin.

Therefore, it is meaningful to include antioxidants in cosmetics to prevent skin damage caused by peroxides in sebum, even if the cosmetics themselves have a composition that does not pose any risk of oxidation. . However, as the carcinogenicity of various chemical substances has been confirmed in recent years, there has been a demand for safer antioxidants in cosmetics.

However, in the course of research on a culture solution obtained by culturing lactic acid bacteria using animal milk as a medium, the present inventors discovered that whey separated from the culture solution was 1,1-diphenyl-2-
It was discovered that it contains a substance that reduces picrylhydrazyl (DPPH) free radicals and suppresses the peroxidation of unsaturated fatty acids such as linoleic acid.

They confirmed that this reducing substance could be used as an antioxidant in cosmetics, and completed the present invention. That is, the present invention provides cosmetics containing an antioxidant obtained by substantially removing high molecular weight substances with a molecular weight of 6,000 or more from whey separated from a lactic acid bacteria culture solution using an animal milk medium,
The present invention also provides cosmetics containing the above antioxidant and tocopherols.

According to what has been confirmed so far, the active ingredient of the above-mentioned oxidizing agent contained in the cosmetics of the present invention has the following properties: (a) Soluble in 90% ethanol; (b) Extremely soluble. Appears in the fraction with a molecular weight of 6,000 or less after filtration; (c) Adsorbed on a strongly acidic cation exchange resin and eluted with aqueous ammonia; (d) Positive for ninhydrin reaction; (e) Maximum absorption at about 275 nm in the ultraviolet region It has a spectrum; (f) From the behavior in gel filtration with BiO-GeIP-2, the molecular weight is thought to be about 1000 or less; (g)
Not a free amino acid; (h) Antioxidant properties are significantly reduced by treatment with the protease enzyme pronase; (1) Acid hydrolysis produces aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, Produces isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine and arginine.
Jiru.

From the above, it appears that the active ingredient of the above-mentioned antioxidant consists of a group of peptide-like substances with a molecular weight of about 1000 or less, but the reason why it exhibits antioxidant properties is not clear.

However, considering the strength of the antioxidant capacity and the fact that the antioxidant capacity is reduced by pronase treatment, at least simple methods that depend on the reducing groups of some of the constituent amino acids It is certain that it is not a thing. Next, the antioxidant ability of the above-mentioned antioxidant will be explained by showing actual measured values.

Table 1 shows the ability of the antioxidant/SE for cosmetics of the present invention, which was produced in the antioxidant production example described later, to suppress the autooxidation of linoleic acid, expressed as DL-α-tocopherol (abbreviated as VE). and butylated hydroxytoluene (B
The test results are shown in comparison with those of HT (abbreviated as HT).

The test was carried out using a linoleic acid ethanolic solution of 1.0 rT11. 10ml of 0.1M phosphate buffer with SpH7.0, 9rn1 of ethanol, 5rr11 of purified water
The amount of peroxide produced was measured by leaving the mixture of antioxidant and antioxidant in a dark place at 36°C.The induction period in the table is:
In the tongue iron method for quantifying peroxide (Mitsuda et al.: R Nutrition and Food Research Vol. 19, No. 3, p. 60), the absorbance increases with the production of peroxide until it reaches 0.3. It is the number of days.

From this test result, the whey-based antioxidant contained in the cosmetics of the present invention shows excellent antioxidant ability even when used alone;
It can be seen that when used in combination with tocopherol, a synergistic effect produces a remarkable effect on the so layer. Table 1 Antioxidants and their concentrations Induction period (days) SE296mg%
+VE6.5μM62 Table 2 also shows the results of examining the oxidation-suppressing effects of the above-mentioned antioxidants on three types of fats and oils that are often used as raw materials for cosmetics.

In this test, an emulsion consisting of 10% oil, 80% SE (aqueous solution with a solid content concentration of 3.7%) (or the same amount of water), and 10% nonionic surfactant was exposed to clean air at 98°C for 2 hours. Blow in at a rate of 2.33n11/sec/20n11 of emulsion, and remove the peroxide in the emulsion before and after using KI-Na2S2.
It was determined by titration with O3 solution. S
It can be seen that E shows excellent antioxidant ability against various fats and oils. As mentioned above, the antioxidant contained in the cosmetics of the present invention is made from whey separated from a lactic acid bacteria culture solution using an animal milk medium. The supernatant may be isolated by filtration or centrifugation from a lactic acid bacteria culture obtained in the same manner as in the production of ordinary lactic acid bacteria drinks, fermented milk, cheese, etc., and no special equipment is required.

However, processing to remove high molecular weight components from whey;
Alternatively, in order to facilitate subsequent purification treatment if necessary, it is desirable to culture lactic acid bacteria using a medium containing skim milk as a main component. In addition, as lactic acid bacteria, Streptococcus thermophilus, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus bulgaricus, etc. are easy to culture and provide whey with high antioxidant capacity.
In addition, it is a particularly preferred bacterial species because it is easy to separate whey from the culture solution. In order to produce the above-mentioned antioxidant from whey, the whey is subjected to a treatment that can substantially remove high molecular weight components having a molecular weight of 6,000 or more.

Although the treatment method is not particularly limited, examples of easy-to-implement methods include an ultrafiltration method and a method of making a 90% alcohol solution and separating the soil solution. Whey as it is before the above treatment still has antioxidant ability, but the molecular weight is 6000, especially 200.
Whey components with a high molecular weight exceeding 0.00 coagulate and precipitate during acid treatment, pH adjustment, alcohol treatment, heat treatment, addition of surfactants, etc. in the cosmetic manufacturing process.
As a result, transparency deteriorates in transparent cosmetics,
In the case of emulsion-based or suspension-based cosmetics, the quality of the cosmetic may be lowered by destabilizing the dispersion system, resulting in deterioration or destabilization of physical properties.

The antioxidant used in the present invention has a molecular weight of 600 from whey.
It does not matter if not only components with a molecular weight of 0 or more, but also peptide substances with a molecular weight of about 2,000 to 6,000, and other whey components unrelated to antioxidant ability (e.g., salts, aroma components, etc.) have been removed; Such a product is superior in terms of antioxidant ability and ease of use.

As a method for obtaining such highly "purified" antioxidants, for example, after removing components with a molecular weight of about 6,000 or more from whey, or from the beginning with a molecular weight of about 2,000 to 60,
After removing components with a higher molecular weight than the molecular weight cut-off, an arbitrary molecular weight within the range of 0.00 is adsorbed on a strongly acidic cation exchange resin (H type), and then the components eluted with alkaline water are captured. There is a way to collect them. Other than using the above-mentioned antioxidant obtained from whey as an antioxidant, the cosmetic product of the present invention is not limited in its type, composition, etc.

In addition, no special technology is required for its production, and the aqueous phase component can be added to the aqueous phase at any stage of the manufacturing process, either as a solution separated from whey or once dried as a powder. It can be added as The preferred addition rate of the whey-based antioxidant to cosmetics is about 0.03 to 3% by weight as solid matter when using a whey-based antioxidant having a purity level of SE level. The present invention does not limit the inclusion of antioxidants other than the whey-based antioxidants in cosmetics.

In particular, tocopherols have a remarkable synergistic effect when used in combination, so it is preferable to coexist in cosmetics using unsaturated fatty acid fats and oils. Tocopherols that can be used here include DL-α-tocopherol,
Examples include DL-α-tocopherol acetate, D-α-tocopherol acetate, DL-α-tocopherol nicotinate, and natural vitamin E mixture. The preferred addition rate of tocopherols to be used in combination is 0.001 to 0.001 to cosmetics.
It is 1% by weight. θ The antioxidant used in the cosmetics of the present invention not only has excellent antioxidant ability as described above, but also
Since it is manufactured from lactic acid fermentation of animal milk without any chemical treatment, it is ideal for use as an antioxidant in cosmetics without worrying about safety. It is something like that.

Therefore, the cosmetics of the present invention are extremely safe as there is no risk of drug damage due to antioxidants, there is almost no risk of skin damage caused by lipid peroxide, and there is also no risk of skin damage caused by peroxidation of sebum. It also has excellent ability to prevent skin disorders. Due to these features, the present invention can be applied not only to cosmetics made from fats and oils containing unsaturated fatty acids, such as creams, milky lotions, and hair conditioners, but also to cosmetics that do not contain fats and oils, such as lotions, and to improve their quality. It is extremely effective in improving safety. The present invention will be explained below with reference to Examples.

Antioxidant Production Example After sterilizing skim milk 1000rT11, it was inoculated with Streptococcus thermophilus and cultured at 37°C for 7 hours.

After the culture was completed, the culture solution was filtered to obtain whey 800rn1. This whey was filtered using an ultrafiltration membrane having a molecular weight cut off of 6000 to obtain an antioxidant SE (34 g as a solid). Separately, the ultrafiltration membrane permeate obtained in the same manner as above was directly passed through a column filled with strongly acidic cation exchange resin Amperlite IR-12B (H type), and then Bo's aqueous ammonia was poured into the column. The components eluted by the flow were collected (yield: 0.7 g after vacuum concentration and drying; hereinafter referred to as antioxidant/SE-R).

Example 1 Lotion ethanol 15n11 polyoxyethylene hydrogenated castor oil? 1,3-butylene glycol 3n11 fragrance/preservative
0.2g antioxidant SE-RO. 5
Example 2 Emulsion Stearic acid 3.0g Behenyl alcohol 1.0g Beef tallow
2.0g olive oil
13.0g Zorbitan monostearate 1
．． 5g Monostearate POE Zorbitan 3.0
gFragrance/preservative 0.3gPG
5.0g DL-α monotocopherol acetate 101
Add 11 g SE to make the total amount 100 rT11.

Claims (1)

[Scope of Claims] 1. Contains an antioxidant obtained by substantially removing high molecular weight substances with a molecular weight of 6000 or more from whey separated from a lactic acid bacteria culture solution using an animal milk medium. cosmetics. 2. A cosmetic product containing an antioxidant and tocopherols obtained by substantially removing high molecular weight substances having a molecular weight of 6,000 or more from whey separated from a lactic acid bacteria culture solution using an animal milk medium.