JP3231075B2 - Cosmetic for preventing skin aging - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic, and more particularly, to a cosmetic for preventing skin aging having an excellent skin aging preventing action and a rough skin improving action.

[0002]

2. Description of the Related Art Pharmaceutical ingredients conventionally used for improving skin roughness and preventing skin aging include allantoin, aloe extract, ginseng extract, placenta extract, bovine blood deproteinizing substances, and fermentation metabolites. Are known. However, cosmetics containing these medicinal components cannot provide a sufficient effect, and there has been a demand for the development of cosmetics having more excellent effects.

On the other hand, anti-inflammatory and anti-allergic effects ("Oil Chemistry", 37, p9, (1988), "Oil Chemistry", 40,
Oils and fats containing eicosapentaenoic acid (hereinafter abbreviated as "EPA") and docosahexaenoic acid (hereinafter abbreviated as "DHA") have attracted attention as compounds having p203, (1991)) and the like. It has long been known that EPA and DHA are contained in fish oil in a large amount and have pharmacological effects such as antithrombotic activity and antiarteriosclerosis activity. In recent years, it has been found that local administration has a vasomotor and vasodilator effect (Japanese Patent Laid-Open No.
-48528).

[0004]

SUMMARY OF THE INVENTION The present inventors have developed an EPA
Focusing on the above-mentioned effects of DHA and DHA, an attempt was made to blend this into cosmetics, especially cosmetics intended to prevent skin aging. However, when EPA, DHA or fats and oils containing EPA or DHA alone are blended alone, the effect of preventing skin aging and the effect of improving skin roughness are not sufficient, and due to the influence of other bases and the like, these substances originally have these effects. It was a fact that the effect could not be fully exerted.

[0005] Furthermore, in order to obtain higher effects, EPA
And / or DHA or fats and oils containing it at a high concentration has a limited effect, and conversely,
There is also a problem in that the production of specific colors and odors of these compounds themselves causes limitations in the product aspect such as the compounding amount and dosage form.

[0006]

Means for Solving the Problems In view of the above-mentioned circumstances, the present inventors have conducted intensive studies in order to fully exploit the effects of EPA and / or DHA or fats and oils containing the same. As a result, it has been found that the combination of these and specific components exerts an excellent effect of preventing skin aging and improving skin roughness in a stable and synergistic manner, and completed the present invention.

That is, the present invention relates to EPA and / or D
HA or fats and oils containing the same and one or more components selected from deoxyribonucleic acid, flavin adenine dinucleotide, yeast extract, bovine blood deproteinized extract, lactic acid bacteria fermentation metabolite and bovine thymus extract It is intended to provide a cosmetic composition for preventing skin aging contained therein.

[0008] As described above, EPA and / or DHA are unsaturated fatty acids contained in fish oils such as sardine oil, saury oil, herring oil, mackerel oil, and cod oil. For example, sardine oil contains about 16% EPA and DHA about 8.4%, and saury oil contains about 4.9% EPA and about 1% DHA.
0.5%, mackerel oil contains about 8.1% EPA and about 1% DHA
0.6%, cod oil about 13.3% EPA, DHA about 1
3.7% each.

The EPA and / or DHA or fats and oils containing DHA used in the present invention may be those separated and purified from fish oil by a conventional method, but may be obtained by simply extracting fish oil. And / or fats and oils containing DHA may be used. The method for extracting fish oil is not particularly limited, and a known method for extracting fats and oils such as a critical gas extraction method, a molecular distillation method, and a liquid chromatography method can be employed.

The fats and oils containing EPA and / or DHA may be used as they are in the cosmetics of the present invention, or may be further purified by steam distillation, activated carbon treatment, acid clay treatment and the like.

The content of EPA and / or DHA in the cosmetic of the present invention is 0.0001 to 10% by weight (hereinafter simply referred to as “%”), and more preferably 0.01 to 10% by weight.
5%. When using a fat or oil having a low purity of EPA and / or DHA, the content of EPA and / or DHA may be within the above range.

When the content of EPA and / or DHA is 0.5%
If it is less than 0001%, a sufficient effect may not be obtained, and even if it exceeds 10%, no further increase in the effect can be seen. Product problems such as precipitation may occur.

On the other hand, in the present invention, deoxyribonucleic acid (hereinafter, referred to as "DNA"), flavin adenine dinucleotide (hereinafter, referred to as "FAD"), yeast extract, bovine blood deproteinized extract, lactic acid bacteria Both the fermented metabolite and the bovine thymus extract (hereinafter collectively referred to as “tissue extract components”) are components that have been conventionally incorporated into cosmetics.

DNA is a substance obtained from animals, plants, and microorganisms, and is a high-molecular-weight polynucleotide in which deoxyribonucleotides are used as units and are polymerized by phosphate groups. As a specific example, for example, “DNA-S”
(Manufactured by Organic Synthetic Chemicals) and the like.

FAD is a substance obtained from animals, plants and microorganisms, and is a dinucleotide comprising riboflavin, two phosphate groups and adenosine. Specific examples include, for example, "FAD" (manufactured by Kyowa Hakko Co., Ltd.).

The yeast extract is, for example, an yeast extract such as baker's yeast or brewer's yeast, or an aqueous extract obtained by subjecting dried and powdered yeast to physical or biochemical means. It contains peptides, organic acids, nucleic acids and the like. Specific examples include "Yeast extract A-33" (manufactured by Asahi Beer).

The bovine blood deproteinized extract is obtained by subjecting the blood of an adult cow or a calf to an appropriate treatment and removing the protein. The production method is not particularly limited, but, for example, the blood as a raw material may be collected from a calf that has activated the intraretinal system, or may be fresh blood from a slaughtered cow. The treatment of bovine blood is performed by heat treatment, enzymatic decomposition, electrolysis, etc., in addition to freezing treatment. The protein can be removed by an ultrafiltration method or a precipitation method, and is not particularly limited as long as it is a commonly used method. As a commercially available bovine blood deproteinizing extract,
"Stimusel" (manufactured by Pentafarm), "Sorcoseryl" (manufactured by Tohoku Pharmaceutical Co., Ltd.) and the like can be preferably used. In the present invention, the bovine blood deproteinized extract can be used as it is or as a solid.

[0018] The fermented metabolite of lactic acid bacteria is produced, for example, by inoculating a lactic acid bacterium into a culture medium mainly composed of animal milk such as milk, performing lactic acid fermentation, and fractionating whey from the obtained culture. . Lactic acid bacteria include Lactobacillus acidophilus, Streptococcus thermophilus, and the like. As a specific example, "Spiron-
L "(manufactured by Sansho Pharmaceutical Co., Ltd.).

The bovine thymus extract is extracted from calf thymus,
A water-soluble extract that does not contain deproteinized hormones and contains various amino acids and low molecular weight proteins. As a specific example, "Sima Septide" (manufactured by Pentafarm) and the like can be mentioned.

The content of these tissue-extracting components in the cosmetic of the present invention is generally 0.0001 to 5%, preferably 0.01%.
01 to 3%. The content of these components is 0.0001
When the amount is less than 5%, a sufficient effect may not be obtained, and when the amount is more than 5%, the effect is almost constant, and no increase is observed.

The cosmetic composition for preventing skin aging according to the present invention may comprise one or two of essential components EPA and / or DHA or fats and oils containing the same and other essential components of a tissue extraction component in accordance with a conventional method. The above can be prepared by blending them in various forms of bases known as ordinary cosmetics.

Examples of the form of the cosmetic are not particularly limited, and may be, for example, cosmetics such as milky lotions, creams, lotions, packs, dispersions, and detergents. , Bases according to the form of these cosmetics, for example,
Purified water, lower alcohols, polyhydric alcohols, oils and fats, surfactants, various cosmetic ingredients, ultraviolet absorbers, thickeners, pigments, preservatives, fragrances, and the like can be used.

[0023]

Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.

EXAMPLES 1-3 Creams: Creams having the compositions shown in Table 1 below were prepared, and the effect of improving skin roughness was examined. The results are also shown in Table 1. In addition,
The method for producing and evaluating the cream is as follows.

(Production method) A. Components (8), (10) to (13) are mixed by heating, and
Keep at 0 ° C. B. Components (1) to (7) and (9) are mixed by heating.
Keep at 0 ° C. C. The above B is added to the above A, mixed and emulsified uniformly. D. After cooling the above C, (14) was added and mixed uniformly to obtain a cream.

(Evaluation method) 105 healthy men were used as panels, and 15 groups were used as groups. The creams of Examples 1 to 3 or Comparative Examples 1 to 4 were applied to one half of the face, and the cream of Comparative Example 5 was applied to the other half. Was applied once a day for 2 weeks. Two weeks later, facial skin replicas were collected and evaluated according to the criteria shown below. The value obtained by subtracting the score of the replica of the cream application site of Comparative Example 5 from the obtained score of the replica of the cream application site of Comparative Examples 1 to 4 was defined as the degree of skin roughness improvement. The score before the test was 1
Or I chose 2 men.

Skin Condition Score: [Score] [State] 1 Skin groove of skin is unclear and exfoliation of keratin is recognized. 2 Skin crevices are slightly unclear or strongly unidirectional. 3 Skin sulcus is recognized, but shallow or slightly unidirectional. 4 Skin sulcus is recognized or slightly reticulated. 5 Skin crevices are clearly recognized or have a fine mesh.

(Result)

[Table 1]

From the above results, it is apparent that the cosmetic of the present invention in which DHA and a tissue extraction component are blended has a remarkable skin roughness improving effect as compared with the case where DHA or a tissue extraction component is blended alone. .

Examples 4 to 5 Creams: Creams were prepared according to the composition shown in Table 2 and by the following method, and their anti-aging effects were examined by the following methods.

[0031] * Kurita Water Industries Ltd. ** DNA-S (Synthetic Organic Chemicals) *** FAD (Kyowa Hakko)

(Production method) A. Components (9) to (11) and (13) are mixed by heating and maintained at 70 ° C. B. Components (1) to (8) are mixed by heating and kept at 70 ° C. C. Add the above B to the above A, mix and emulsify uniformly. D. After cooling the above C, (12) was added and mixed uniformly to obtain a cream.

(Evaluation Method) An appropriate amount of the test cream was applied to the face of a panel of 15 women aged 26 to 52 years after washing the face twice a day, morning and night, for 12 weeks. The skin and skin aging prevention effects of the application were evaluated according to the following criteria.

Skin effect: [Evaluation] [Contents] Effective Skin dullness became inconspicuous. Slightly effective The dullness of the skin became slightly less noticeable. Ineffective No change from before use.

Anti-aging effect of skin: [Evaluation] [Contents] Effective The skin was improved in adhesion and gloss. Slightly effective Skin glue, gloss slightly improved. Ineffective No change from before use.

(Results) The results are shown in Table 3.

[0037]

As is evident from the results in Table 3, the creams of Examples 4 and 5 are effective in preventing and improving the occurrence of "glossy" and "bleeding" of the skin, preventing skin aging. The effect was recognized.

Example 6 Cosmetic Water: <Formulation> (%) (1) Polyoxyethylene hydrogenated castor oil (60E.O.) 1.0 (2) Ethyl alcohol 10.0 (3) Preservative 1 (4) Appropriate amount of fragrance (5) Sardine extracted oil and fat 1.0 (6) Fermented metabolite of lactic acid bacteria ^* 0.5 (7) Sorbitol (70% aqueous solution) 3.0 (8) Sodium pyrrolidone carboxylate 3.0 ( 9) Refined water balance * Spiron-L (manufactured by Sanshou Pharmaceutical Co., Ltd.)

(Production Method) A. Components (1) to (5) are heated and mixed and dissolved. B. Heat and mix and dissolve components (6)-(9). C. The above A and B were mixed to make uniform, and a lotion was obtained.

Example 7 Emulsion: <Formulation> (%) (1) Polyoxyethylene sorbita 1.0 monostearate (10E.O.) (2) Polyoxyethylene sorbite 0.5 tetratetraole Ethate (60E.O.) (3) Glyceryl monostearate 1.0 (4) Stearic acid 0.5 (5) Behenyl alcohol 0.5 (6) Refined avocado oil 4.0 (7) Tri-2-ethylhexanoic acid G 4.0 Lyserin (8) Preservative 0.1 (9) DHA ^* 1.0 (10) Witch hazel extract 0.1 (11) Xanthan gum (2% aqueous solution) 7.0 (12) 1,3-butylene Glycol 5.0 (13) Bovine thymus extract ^** 1.0 (14) Yeast extract ^*** 1.0 (15) Residual water (16) Appropriate perfume * Serdary Research Lab. Inc. * * Cymaspeptide (Pentafirm) *** Yeast extract A-33 (Asahi Beer)

(Preparation method) A. Components (10) to (15) are mixed by heating and maintained at 70 ° C. B. Components (1) to (9) are mixed by heating and kept at 70 ° C. C. Add the above B to the above A, mix and emulsify uniformly. D. After cooling the above C, the component (16) was added and mixed uniformly to obtain an emulsion.

[0043]

According to the present invention, EPA and / or DH
A or a fat or oil containing the same is blended to obtain a cosmetic having a stable and excellent skin aging prevention effect and a skin roughness improving effect.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-2-48528 (JP, A) JP-A-59-134706 (JP, A) JP-A-64-75423 (JP, A) JP-A-58-1983 192811 (JP, A) JP-A-63-188607 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K 7/00 A61K 7/48

Claims (1)

(57) [Claims] 1. Eicosapentaenoic acid and / or docosahexaenoic acid or fats and oils containing the same, deoxyribonucleic acid, flavin adenine dinucleotide, yeast extract, bovine blood deproteinized extract, fermented metabolite of lactic acid bacteria and bovine thymus extract A skin aging prevention cosmetic comprising one or more components selected from the group consisting of: