JPS6032819B2 - Absorbance analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an absorbance analyzer equipped with a two-wavelength spectrometer and particularly effective for quantitative analysis of complex sample components, such as biological samples.

More specifically, the present invention is an apparatus for measuring the absorbance of a large number of sample containers by sequentially transporting these containers to measurement positions, in which light from a light source is split into two wavelengths of light at the rear of the sample container transport path. a two-wavelength spectrometer, a differential amplifier that calculates the difference in the absorbance of each wavelength light from the two-wavelength spectrometer, a position detection means that detects whether or not a sample container is transferred to a measurement position; A circuit for storing the absorbance signal from the differential amplifier before the sample container is transferred to the measurement position based on the signal from the detection means and a circuit for storing the absorbance signal from the differential amplifier before the sample container is transferred to the measurement position based on the signal from the position detection means. The present invention relates to an absorbance analyzer comprising: a calculation unit that calculates the difference between the absorbance signal from the differential amplifier and the absorbance signal of the storage circuit when A method of measuring absorbance is known as one of the methods for quantifying substances. This absorbance is affected by changes in color temperature or the like of the light source, which may impede accurate measurement. For example, a light source generally requires time for its color temperature to change. Therefore, especially when you want to take measurements quickly, that is, when you want to take measurements soon after lighting, there is a large error caused by changes in the color temperature of the light source. The present invention has been made in view of these circumstances.

Simply speaking, the features of the absorbance analyzer according to the present invention are as follows:
In the two-wavelength side light system, a means for correcting changes in the color temperature of the light source is attached to the calculation section directly in the analyzer.

In other words, its feature lies in the provision of means for automatically subtracting the absorbance (or absorbance difference) due only to the light source from the absorbance (or absorbance difference) of the sample. This enables more accurate quantitative analysis based on absorbance, for example, immediately after lighting or when the color temperature of the light changes due to some influence. The present invention will be described in detail below based on embodiments shown in the figures.

Note that the present invention is not limited by this.
In FIG. 1, reference numeral 1 denotes a dual-wavelength absorbance automatic analyzer, which includes a large number of sample containers S, . A rotating logical conveyor 2 that supports S2... at intervals, a light source 4 and a lens 5 facing each other at the sample measurement position 3, and a two-wavelength spectrometer 6 connected in order to the outside of this lens. , detectors 7 and 8, logarithmic conversion circuits 9 and 10, a differential amplifier 11, a main processing circuit 13 comprising a correction circuit 12 for color temperature change of the light source 4 and a calculation circuit 24, and an indicator (display) meter. It is mainly composed of 14.

The rotating ring conveyor 2 includes sample containers S, S2...
The through-hole days,, ratios, etc. for detecting that the sample containers S, S2, etc. are sent to the set measurement positions 3, respectively, are provided in advance, and the sample containers S, S2, etc. Through hole B for detecting the position where the light east directly faces the lens 5
, , B2... are provided in advance.

This rotary static conveyor 2 can be continuously rotated by suitable power means. The two-wavelength spectrometer 6 is composed of a mirror 15, a semi-transparent mirror 16, and diffraction gratings 17 and 18, and these two diffraction gratings separate the light from the light source 4 into two wavelengths: 1 and 2.

The color temperature change correction circuit 12 includes the differential amplifier 11
A peak detection gate 19 and a memory circuit 20 extend from the output side of the
a gate circuit 21 connected in series, a gate circuit 23 branched from the output side and connected to the light source monitor detection gate 22, and the other ends of both circuits 21 and 23 connected to the input side, respectively, and the output side connected to the Differential amplifier 2 connected to calculation circuit 24
It is mainly composed of 5.

The peak detection gate 19 is connected to the peak detection photocoupler 26, which serves as a position detection section, arranged as appropriate. On the other hand, the light source monitor detection gate 22 receives the presence/absence detection signal 27 of day 2..., that is, the gate signal, and the presence/absence (10) of the through holes B, B2..., ( one)
It is set so that a detection signal 29, that is, a gate signal, is input. Note that the arithmetic section for detecting the difference between the absorbance signal associated with the sample and the signal caused by fluctuations in the light source in the dual-wavelength absorbance automatic analyzer 1 includes not only the main arithmetic circuit 13 but also the logarithmic conversion circuits 9 and 10 and the differential amplifier. It includes a middle part 11.

Next, the operation of the automatic analyzer 1 having the above configuration will be explained. First, the light source 4 is turned on and the rotary ring conveyor 2 starts rotating.

For example, if we start by explaining the movement of the sample container S to the sample measurement position 3, this movement can be indirectly explained as follows:
is detected by the peak detection photocoupler 26, and a presence (10) signal is transmitted to the peak detection gate 19. Of course, at this time, the (10) signal is not transmitted to the light source monitor detection gate 22. On the other hand, Koto, which has passed through the sample container S, passes through the mirror 15, the semi-transparent mirror 16, the diffraction grating 17,
18, the light is separated into two wavelengths: I, and I2, and the two wavelengths are further calculated into the absorbance difference between the two wavelengths by detectors 7 and 8, logarithmic conversion circuits 9 and 10, and an intensifier 11. This is transmitted to both circuits 21 and 23 of the color temperature change correction circuit 12.

However, as described above, since the present (10) signal is transmitted only to one peak detection gate 19, only that circuit 21 becomes conductive, and the absorbance difference between the two wavelengths is stored in the storage circuit 20'. Subsequently, the rotary conveyor 2 continues to rotate and the light source 4
When the light east reaches the point directly toward the lens 5, the light source monitor detection photocoupler 28 detects the through-hole ear and detects its presence (
10) Signal to light source monitor detection gate 22 [and memory circuit 2
0]. Thus, the absorbance difference between the two wavelengths appearing on the output side of the differential intensifier 11 and the absorbance difference in the storage circuit 20 at that time are simultaneously transmitted to the input side of the pen intensifier 25 and are intensified. Then, it passes through the calculation wax 24 and is calculated to a desired concentration value, which is displayed on the display meter 14 as appropriate. In other words, by increasing the difference scale using the difference intensifier 25 described above, it becomes possible to perform correction by subtracting the absorbance difference of the light source itself from the absorbance difference due to the sample. Therefore, Honso Nao 1 is effective when the color temperature change of the light source is large and affects the outside of the measurement data, and is especially effective when the measurement is performed immediately after the light source V is turned on because the color temperature change is large. . In the above embodiment,
Memory circuit 201 or circuit 2 equipped with peak detection gate 19
1, a storage circuit with the same content may be connected to a circuit provided with a light source monitor detection gate.

For example, if this change is made to the circuit 12 in FIG. 1, correction of color temperature change of the light source will be performed for the light source before sample measurement (peak). Furthermore, it is more preferable to correct the color temperature change of the light source using the average value of the values before and after the sample measurement. This example is shown in the second
As shown in the figure, 31a is an average value calculation circuit, and 32a is a signal distributor. In the above embodiment, the two-wavelength absorbance signal obtained by the detector and the logarithmic conversion circuit is converted into a two-wavelength absorbance difference (signal) via the reed intensifiers 11 and 11a, and then sent to the light source color temperature change correction circuit 12. , 12a, but as shown in FIG. 3, the respective two-wavelength absorbances (
signal) directly to the color temperature change correction circuit 12' of each light source.
b, 12″b, and then determine the two-wavelength absorbance using the differential intensifier 11b.The automatic analyzer 1, la, and lb shown in FIGS. 1 to 3 measure the difference in absorbance at two wavelengths. However, when measuring single wavelength absorbance, the color temperature change correction circuit 12, 12a, 12' of the light source is also required.
A correction circuit having the same configuration as that of b and 12rb can be attached. In the embodiment, the gate signals, that is, the peak detection signal and the light source monitor signal, are obtained directly by the photocoupler of the position detection section, but instructions for transmitting both signals are stored in advance in an appropriate computer in accordance with the movement of the conveyor. It may also be obtained indirectly.

[Brief explanation of drawings]

FIG. 1 is a functional explanatory diagram showing one embodiment of an automatic analyzer according to the present invention, and FIGS. 2 and 3 are diagrams corresponding to FIG. 1 of other embodiments, with some parts omitted. 1... Automatic analyzer, 3... Setting measurement position, 4... Light source, 6... Dual wavelength spectrometer, 7, 8... ...Detector, 9,10...
Logarithmic conversion circuit, 12... Light source color temperature correction circuit, 13... Arithmetic circuit, 14... Indicator, 20... Memory circuit, 21, 23...
Gate circuit, 25... Differential multiplier, 27...
...Peak detection signal, 29...Light source monitor signal. Figure 2 Figure 1 Figure 3

Claims (1)

[Claims] 1. In a device that measures the absorbance of a large number of sample containers by sequentially transporting these containers to a measurement position, a dual-wavelength spectrometer is used that separates light from a light source into two wavelengths of light at the rear of the sample container transport path. , a differential amplifier that calculates the difference in absorbance of each wavelength light beam from the two-wavelength spectrometer, a position detection means that detects whether or not the sample container is transferred to the measurement position, and a position detection means based on the signal of this position detection means. a circuit for storing the absorbance signal from the differential amplifier before the sample container is transferred to the measurement position; and a circuit for storing the absorbance signal from the differential amplifier when the sample container is transferred to the measurement position based on the signal from the position detection means. 1. An absorbance analyzer comprising: a calculation unit that calculates a difference between an absorbance signal from the storage circuit and an absorbance signal from the storage circuit; and a display unit for displaying the calculation output.